##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527475_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680533
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.028570253022263	31.0	31.0	33.0	30.0	34.0
2	31.27176786430636	31.0	31.0	34.0	30.0	34.0
3	31.66914462634435	33.0	31.0	34.0	30.0	34.0
4	35.75910793451603	37.0	35.0	37.0	33.0	37.0
5	34.118069219273714	35.0	35.0	37.0	30.0	37.0
6	35.583270759830896	36.0	35.0	37.0	35.0	37.0
7	36.03285219085628	37.0	35.0	37.0	35.0	37.0
8	36.40110325289148	37.0	37.0	37.0	35.0	37.0
9	38.545930910036695	39.0	39.0	39.0	37.0	39.0
10	37.17218268621801	39.0	37.0	39.0	34.0	39.0
11	36.879855936449815	39.0	37.0	39.0	32.0	39.0
12	36.671774917601354	39.0	35.0	39.0	32.0	39.0
13	36.678582816703965	39.0	35.0	39.0	33.0	39.0
14	37.51526377119111	39.0	37.0	40.0	33.0	40.0
15	37.59307924817753	40.0	37.0	40.0	33.0	40.0
16	37.65349365864697	40.0	37.0	40.0	33.0	40.0
17	37.574073557050134	39.0	37.0	40.0	33.0	40.0
18	37.46351609694166	39.0	36.0	40.0	33.0	40.0
19	37.30720185501658	39.0	36.0	40.0	32.0	40.0
20	37.14316278564008	39.0	36.0	40.0	32.0	40.0
21	37.20872757088929	39.0	36.0	40.0	32.0	40.0
22	37.158763792497936	39.0	36.0	40.0	32.0	40.0
23	37.079195277819004	39.0	36.0	40.0	32.0	40.0
24	37.01764205409583	39.0	35.0	40.0	32.0	40.0
25	37.007752746744096	39.0	35.0	40.0	32.0	40.0
26	36.8674098096639	39.0	35.0	40.0	32.0	40.0
27	36.78033247469263	38.0	35.0	40.0	31.0	40.0
28	36.562786815628336	38.0	35.0	40.0	31.0	40.0
29	36.51066002677313	38.0	35.0	40.0	31.0	40.0
30	36.354813065641196	38.0	35.0	40.0	31.0	40.0
31	36.29463376500478	38.0	35.0	40.0	31.0	40.0
32	36.1426528911897	38.0	35.0	40.0	30.0	40.0
33	35.88893852318697	38.0	34.0	40.0	30.0	40.0
34	35.51645254528436	38.0	34.0	40.0	29.0	40.0
35	35.502650128649165	38.0	34.0	40.0	29.0	40.0
36	35.2900976146638	37.0	34.0	40.0	29.0	40.0
37	35.23439862578303	37.0	34.0	39.0	29.0	40.0
38	35.093337134275636	37.0	33.0	39.0	28.0	40.0
39	34.91622595818278	36.0	33.0	39.0	28.0	40.0
40	35.1873340455202	37.0	34.0	39.0	29.0	40.0
41	35.32518334893385	37.0	34.0	40.0	30.0	40.0
42	35.36702261315763	36.0	34.0	40.0	30.0	40.0
43	35.322363500373974	36.0	34.0	40.0	30.0	40.0
44	35.21071424897838	36.0	34.0	39.0	30.0	40.0
45	35.08393127151806	35.0	34.0	39.0	30.0	40.0
46	34.75649527649651	35.0	34.0	39.0	29.0	40.0
47	34.7130352238613	35.0	34.0	39.0	29.0	40.0
48	34.53400349431989	35.0	33.0	39.0	29.0	40.0
49	34.367076982306514	35.0	33.0	38.0	29.0	40.0
50	34.204172317874374	35.0	33.0	38.0	28.0	40.0
51	34.06691960566203	35.0	33.0	38.0	28.0	40.0
52	33.57669650112485	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	3.0
14	16.0
15	27.0
16	45.0
17	73.0
18	146.0
19	239.0
20	463.0
21	727.0
22	1164.0
23	1685.0
24	2304.0
25	3330.0
26	4442.0
27	5969.0
28	7947.0
29	10764.0
30	14060.0
31	18324.0
32	25607.0
33	37712.0
34	61660.0
35	73648.0
36	111691.0
37	145460.0
38	141915.0
39	11108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.24441577410647	0.6037914399448668	0.0881661873854758	0.06362659856318503
8	99.49363219711609	0.3675060577517916	0.08875396196804564	0.05010778316407874
9	98.2609219538215	1.1025181732553748	0.16854436155190122	0.468015511371234
10	64.65358770258018	26.036944571387426	4.014941229888926	5.2945264961434635
11	34.172185625090925	25.216852085056864	20.581073952328545	20.029888337523676
12	29.142451578395168	16.409931627121683	27.75001359228722	26.697603202195925
13	23.083818124910916	17.865261493564603	29.500700186471484	29.550220195052994
14	24.91091541482926	17.874812830531365	30.930461858572617	26.283809896066764
15	25.239922237422725	18.958081386207574	26.909055108275425	28.892941268094276
16	31.157048960153293	18.021609532528178	24.586904676187636	26.234436831130893
17	31.216120305701562	20.469396781640274	22.29605324062169	26.018429672036476
18	29.682175588839925	20.542427773524576	22.403909876523254	27.371486761112244
19	31.10429619136765	19.084746808751376	21.87021055554984	27.940746444331133
20	30.68447819576714	20.02518614086312	22.812119324118008	26.47821633925173
21	31.913661791566316	18.352379678869358	24.523718908561378	25.21023962100295
22	32.30232773429062	17.82088451258058	23.786355694727515	26.09043205840128
23	31.815356492631512	18.932954022802715	22.79610246674298	26.455587017822797
24	30.23394897822736	19.53130854785881	23.106153559048572	27.128588914865258
25	27.590579736765154	20.269553423566528	23.54698449597595	28.592882343692374
26	28.19628144410337	21.955731757313753	22.146611553003307	27.70137524557957
27	29.178011940640648	18.938390937691484	21.820984434259614	30.06261268740825
28	29.56197568670439	20.443240812715917	21.135051496400614	28.85973200417908
29	29.909056577711883	19.808003432603563	21.404693086154527	28.878246903530027
30	28.36644218575734	20.143328831959657	22.45519320885247	29.035035773430533
31	33.64451099358885	20.05295848988954	20.24339745464217	26.059133061879436
32	34.14514799429271	19.203624218076126	21.30271419607866	25.348513591552503
33	32.17992367747045	19.400675646882664	22.243447415481686	26.175953260165198
34	28.67458301066958	23.314519648569576	24.180164665049304	23.830732675711538
35	31.213181432788712	23.409886074591533	22.811384605889796	22.565547886729963
36	30.391913397293003	24.12844050178316	20.55903240548217	24.920613695441663
37	31.09900622012452	23.087932546988903	20.438244728764072	25.374816504122506
38	32.03650667932341	23.60443946142215	21.312412476691065	23.046641382563372
39	32.33656560372531	20.869230441433405	21.408660564586874	25.385543390254405
40	29.54066885808623	20.920366830116983	22.124276118865655	27.41468819293113
41	28.276365730978515	22.307808732273084	21.80966977354515	27.606155763203255
42	27.592343060512864	19.179084629253833	23.427960143005556	29.800612167227747
43	27.144605772240286	18.503143859298522	25.849885310484577	28.502365057976615
44	26.876286675297155	19.210824456712604	25.077402565342165	28.83548630264807
45	28.203922513676783	18.813048007958468	25.169830118451276	27.813199359913483
46	28.74937732630159	19.513969197673	23.612962192869414	28.123691283156
47	27.728412876377778	20.374030355618316	24.567067284025903	27.330489483978
48	28.352335595775664	20.67585260376793	24.946916608011662	26.024895192444745
49	28.199661147953147	19.699999853056354	24.98188919567457	27.11844980331593
50	25.427863160199433	22.243153528190405	24.532535527299927	27.796447784310242
51	26.947113512496824	21.4627358261833	24.03395573763506	27.556194923684817
52	28.89867207027433	20.0791144588139	23.42413960821885	27.598073862692917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.5
6	5.0
7	3.5
8	2.0
9	35.5
10	69.0
11	71.5
12	74.0
13	79.0
14	126.0
15	168.0
16	207.5
17	247.0
18	312.5
19	378.0
20	345.5
21	313.0
22	499.5
23	686.0
24	744.5
25	803.0
26	1312.5
27	1822.0
28	2002.0
29	2182.0
30	2564.0
31	2946.0
32	3267.0
33	3588.0
34	4517.0
35	5446.0
36	5514.5
37	5583.0
38	6957.5
39	9207.0
40	10082.0
41	10697.5
42	11313.0
43	12432.5
44	13552.0
45	16080.5
46	18609.0
47	19036.5
48	19464.0
49	23665.0
50	27866.0
51	29370.0
52	30874.0
53	35067.0
54	39260.0
55	41596.0
56	43932.0
57	51599.5
58	59267.0
59	63696.5
60	68126.0
61	65664.5
62	63203.0
63	64006.5
64	59200.5
65	53591.0
66	45821.0
67	38051.0
68	33276.5
69	28502.0
70	22893.0
71	17284.0
72	16305.0
73	15326.0
74	14821.0
75	14316.0
76	10785.0
77	7254.0
78	4597.0
79	1940.0
80	1333.5
81	727.0
82	491.0
83	255.0
84	176.5
85	98.0
86	85.0
87	72.0
88	49.5
89	15.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	680533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.32798262538334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.37586641461878	6.378088939110961
2	10.464713295526149	1.952293276005719
3	3.848456206679269	1.0769499789135868
4	1.7674858223062382	0.6594830816433589
5	0.9499054820415879	0.4430350916120158
6	0.5923125393824826	0.331504864569389
7	0.4206049149338374	0.27463767370575715
8	0.3056080655324512	0.22805653803709738
9	0.24732199117832387	0.20763137129279552
>10	7.038437303087587	19.04668840452998
>50	4.0879017013232515	26.711562848531962
>100	1.7785129174543164	30.088474769041323
>500	0.08506616257088848	5.073376309451563
>1k	0.03780718336483932	7.528216853554494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4296	0.6312699016800067	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3936	0.5783701892487212	No Hit
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3424	0.5031350426797819	No Hit
TTGGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2909	0.42745906517391513	No Hit
TTGGACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2719	0.3995397725018478	No Hit
TTGGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2697	0.3963070122977137	No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2621	0.3851392952288868	No Hit
TTGGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2566	0.3770573947185515	No Hit
TTGGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2098	0.30828776855788037	No Hit
TTGGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2094	0.3076999939753105	No Hit
TTGGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1992	0.2927117421197797	No Hit
TTGGACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1982	0.29124230566335507	No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1894	0.2783112648468186	No Hit
TTGGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1893	0.2781643212011761	No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1805	0.26523328038463967	No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1634	0.2401059169797791	No Hit
TTGGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1626	0.23893036781463942	No Hit
TTGGACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	1506	0.22129713033754425	No Hit
TTGGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1499	0.22026852481804704	No Hit
TTGGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1403	0.2061619348363709	No Hit
TTGGACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1383	0.20322306192352171	No Hit
TTGGACGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG	1122	0.16487077041083972	No Hit
TTGGACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1077	0.15825830635692906	No Hit
TTGGACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	1056	0.1551724897984374	No Hit
TTGGACGGGGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCA	905	0.132983999306426	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	893	0.13122067555871647	No Hit
TTGGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	854	0.12548987337866055	No Hit
TTGGACGGGACTATATATAATTATTTCTAAAGCACAGATTAAGTAAGTTCTG	845	0.1241673805678784	No Hit
TTGGACGGGATGTATATGTGTGCATTTGTCAGTACTGTGTGTGTGTCTTTGT	832	0.12225711317452645	No Hit
TTGGACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	802	0.11784880380525264	No Hit
TTGGACGGGACTCTGGGGTGGTGCTTAGCCGGCGCCAGACCGACCCTCGGCT	797	0.11711408557704035	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	793	0.11652631099447051	No Hit
TTGGACGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCT	790	0.11608548005754313	No Hit
TTGGACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	773	0.11358743808162132	No Hit
TTGGACGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	768	0.11285271985340903	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	727	0.10682803038206817	No Hit
TTGGACGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG	711	0.10447693205178882	No Hit
TTGGACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	701	0.10300749559536422	No Hit
TTGGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	700	0.10286055194972175	No Hit
TTGGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	694	0.10197889007586701	No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	692	0.10168500278458209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005583858534413467	0.0	0.0	0.0	0.0
8	0.005583858534413467	0.0	0.0	0.0	0.0
9	0.005583858534413467	0.0	0.0	0.0	0.0
10	0.011314660714469394	0.0	0.0	0.0	0.0
11	0.01733935018581024	0.0	0.0	0.0	0.0
12	0.025274307050503063	0.0	0.0	0.0	0.0
13	0.03320926391519588	0.0	0.0	0.0	0.0
14	0.040409502551676406	0.0	0.0	0.0	0.0
15	0.04775668483379939	0.0	0.0	0.0	0.0
16	0.05583858534413467	0.0	0.0	0.0	0.0
17	0.06098161294162076	0.0	0.0	0.0	0.0
18	0.0684757388693862	0.0	0.0	0.0	0.0
19	0.0761168084427941	0.0	0.0	0.0	0.0
20	0.08155372333156512	0.0	0.0	0.0	0.0
21	0.08625591999212381	0.0	0.0	0.0	0.0
22	0.09007645477882777	0.0	0.0	0.0	0.0
23	0.09551336966759878	0.0	0.0	0.0	0.0
24	0.10050945361944241	0.0	0.0	0.0	0.0
25	0.10550553757128604	0.0	0.0	0.0	0.0
26	0.11226494527083918	0.0	0.0	0.0	0.0
27	0.11667325464011297	0.0	0.0	0.0	0.0
28	0.12416738056787842	0.0	0.0	0.0	0.0
29	0.12945735181100695	0.0	0.0	0.0	0.0
30	0.1376861959669847	0.0	0.0	0.0	0.0
31	0.14385782908396802	0.0	0.0	0.0	0.0
32	0.15429082792458265	0.0	0.0	0.0	0.0
33	0.15972774281335364	0.0	0.0	0.0	0.0
34	0.1664871505129068	0.0	0.0	0.0	0.0
35	0.17530376925145438	0.0	0.0	0.0	0.0
36	0.18279789517921982	0.0	0.0	0.0	0.0
37	0.1908797956895551	0.0	0.0	0.0	0.0
38	0.198520865262963	0.0	0.0	0.0	0.0
39	0.20601499119072844	0.0	0.0	0.0	0.0
40	0.21189273701642683	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTAGT	35	1.0187432E-7	46.000004	40
GACCGTT	35	1.0187432E-7	46.000004	9
GGCGTAA	35	1.0187432E-7	46.000004	34
GTCTATG	35	1.0187432E-7	46.000004	10
TATTGGA	35	1.0187432E-7	46.000004	44
AACGCAC	35	1.0187432E-7	46.000004	36
CTCATAA	75	0.0	46.000004	37
TCGTACC	35	1.0187432E-7	46.000004	38
ACGTCTA	35	1.0187432E-7	46.000004	23
CATCGAA	35	1.0187432E-7	46.000004	25
GCAATCT	35	1.0187432E-7	46.000004	24
GCGTAAC	35	1.0187432E-7	46.000004	35
TTCTATC	20	6.30963E-4	46.0	44
GTTGACG	20	6.30963E-4	46.0	28
GCCTTAT	20	6.30963E-4	46.0	20
TCGCAAT	20	6.30963E-4	46.0	43
TACCCAC	30	1.860275E-6	46.0	29
CGAACAC	20	6.30963E-4	46.0	32
CGAACAA	20	6.30963E-4	46.0	25
CGTATAT	55	1.8189894E-12	46.0	40
>>END_MODULE