FastQCFastQC Report
Fri 17 Jun 2016
SRR1527474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377556
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAA26760.7087690302895464No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT21530.5702465329646463No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG15720.4163620760893748No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC15660.4147729078600261No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGA15590.4129188782591192No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC13900.3681573064657958No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC13400.35491423788788945No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG12260.324720041530263No Hit
TCACTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC9350.24764538240684827No Hit
TCACTTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT9300.24632107554905763No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC8850.23440231382894192No Hit
TCACTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA8630.22857536365466316No Hit
TCACTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG8550.22645647268219812No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC8450.2238078589666169No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC8160.2161268791914312No Hit
TCACTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA7760.20553242432910618No Hit
TCACTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC7590.201029781012618No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA7530.19944061278326924No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC7280.1928190784943161No Hit
TCACTTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA6800.180105732659526No Hit
TCACTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG6750.17878142580173537No Hit
TCACTTGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTC6720.177986841687061No Hit
TCACTTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC6670.17666253482927036No Hit
TCACTTGGGAGTGCCATCCAGGAGGAACCCCCCTGGCGGCAAGTCCAGCC6250.16553835722382904No Hit
TCACTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT5960.15785737744864337No Hit
TCACTTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT5900.1562682092192946No Hit
TCACTTGGGGGCTGAAGATGAGGAGGGCTACCGCAAACTCATTGACCAGA5760.15256015001748086No Hit
TCACTTGGGCCGGGCATTCGGCAGTTCCTGCTCACTAACCTGGTAGGTGT5600.1483223680725508No Hit
TCACTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA5420.14355486338450454No Hit
TCACTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT5300.14037652692580702No Hit
TCACTTGGGGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCA5280.13984680418269077No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5250.1390522200680164No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACT4900.12978207206348197No Hit
TCACTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA4870.1289874879488076No Hit
TCACTTGGCGGATGTGAGGTGTGGTTGTCCAGCTTTTGCCAAAGCGGAAA4730.12527942874699383No Hit
TCACTTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTC4700.12448484463231943No Hit
TCACTTGGGGGTGGCCACGTGGCGCACGCGCAGTGCGGAATTGTAGGTCT4670.12369026051764506No Hit
TCACTTGGGGGAGACGGCTCAGGGTAGTCGGAGTTTGGGAGCTTTGCGCG4440.11759844897180816No Hit
TCACTTGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCA4410.11680386485713379No Hit
TCACTTGGGGTGCTTCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTA4390.11627414211401751No Hit
TCACTTGGGGAGTGCGGTGCTGTGAGAAGTATCGGTGGGAGCTACGCTGA4240.11230122154064562No Hit
TCACTTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT4220.11177149879752937No Hit
TCACTTGGGGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCATCA4190.110976914682855No Hit
TCACTTGGGATTAACACCTTGCTACTCTGGGTCTCATCTCTCTGGCACAT4180.11071205331129688No Hit
TCACTTGGGAGGCGGTGGTGGCGCACTCCTTTGATCCCAGCACTTGGGAG4140.10965260782506436No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC4090.10832830096727372No Hit
TCACTTGGGATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGAAA4080.1080634395957156No Hit
TCACTTGGGGGATTTGGAGGACTGAAGGAAGATGGGTCAAAGCCAGAGTG4070.10779857822415748No Hit
TCACTTGGGGAGAGACCCTGTATCAAAAAAAGAGAGAGAGAGAGAGAGGG4030.10673913273792499No Hit
TCACTTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4030.10673913273792499No Hit
TCACTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG3980.10541482588013434No Hit
TCACTTGGGGAGGATGAATCTGAAGATTCCTGAAGGCCAGAATCCTTAGC3980.10541482588013434No Hit
TCACTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT3970.10514996450857622No Hit
TCACTTGGGGTGCAGTCCTTCACTTGGGATTCTAAAAAAGGGAGATAAAA3920.10382565765078558No Hit
TCACTTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC3920.10382565765078558No Hit
TCACTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC3910.10356079627922746No Hit
TCACTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG3880.10276621216455309No Hit
TCACTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC3830.10144190530676245No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG3810.1009121825636462No Hit
TCACTTGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGT3800.10064732119208807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT207.849757E-444.00000411
AGGTATG408.287316E-944.00000430
TGTCAAA207.849757E-444.00000428
TTAGGAC207.849757E-444.00000439
CAAACCG207.849757E-444.00000430
CTCAATT207.849757E-444.00000417
AGATAAA207.849757E-444.00000443
ACCATCG207.849757E-444.00000444
CTATCCC207.849757E-444.00000442
TTAGCCG207.849757E-444.00000414
TTATGTT207.849757E-444.00000432
CGCATGA408.287316E-944.00000425
ATAGCCA207.849757E-444.00000443
CGACCTA207.849757E-444.00000421
CCAATAG408.287316E-944.00000444
CCAATAC207.849757E-444.00000436
ATTAACG207.849757E-444.00000413
GTGATAC408.287316E-944.00000418
TTCGTCC207.849757E-444.00000420
ATTCGCC207.849757E-444.00000411
Produced by FastQC (version 0.11.5)