FastQCFastQC Report
Fri 17 Jun 2016
SRR1527473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297881
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAA19690.6610022122928283No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG17780.5968826477687398No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG13530.45420822408948536No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA13070.4387658158794955No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT12000.4028454315649538No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC11970.4018383179860414No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10890.36558222914519556No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG9690.3252976859887002No Hit
TCACTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG8350.280313279463947No Hit
TCACTTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA7540.2531212128333126No Hit
TCACTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA7430.2494284630439672No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC7380.24774994041244658No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC7190.24137155441266817No Hit
TCACTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA6960.2336503503076732No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT6800.22827907788680715No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG6730.22592914620267823No Hit
TCACTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC6720.22559344167637413No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC6180.20746539725595123No Hit
TCACTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG5920.19873707957204387No Hit
TCACTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG5870.1970585569405232No Hit
TCACTTGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGT5670.19034446641444067No Hit
TCACTTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA5350.17960192157270854No Hit
TCACTTGGGAGTGCCATCCAGGAGGAACCCCCCTGGCGGCAAGTCCAGCCTG5320.17859480799379618No Hit
TCACTTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG5000.16785226315206406No Hit
TCACTTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG4920.16516662694163106No Hit
TCACTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG4820.16180958167858978No Hit
TCACTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG4540.15240985494207418No Hit
TCACTTGGGCCGGGCATTCGGCAGTTCCTGCTCACTAACCTGGTAGGTGTGG4470.1500599232579453No Hit
TCACTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT4250.14267442367925448No Hit
TCACTTGGGGGTGGCCACGTGGCGCACGCGCAGTGCGGAATTGTAGGTCTAC4210.14133160557403796No Hit
TCACTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC4160.1396530829425173No Hit
TCACTTGGGGTGCTTCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG4090.13730315125838843No Hit
TCACTTGGGGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAAC4070.13663174220578017No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT3950.13260328789013062No Hit
TCACTTGGGGGCTGAAGATGAGGAGGGCTACCGCAAACTCATTGACCAGAAG3900.13092476525861No Hit
TCACTTGGCGGATGTGAGGTGTGGTTGTCCAGCTTTTGCCAAAGCGGAAACG3770.1265606064166563No Hit
TCACTTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA3640.12219644757470265No Hit
TCACTTGGGAGGCGGTGGTGGCGCACTCCTTTGATCCCAGCACTTGGGAGGC3580.12018222041687789No Hit
TCACTTGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT3580.12018222041687789No Hit
TCACTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG3520.11816799325905313No Hit
TCACTTGGGGGAGACGGCTCAGGGTAGTCGGAGTTTGGGAGCTTTGCGCGTG3510.11783228873274898No Hit
TCACTTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGG3460.11615376610122834No Hit
TCACTTGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACG3440.11548235704862009No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC3430.11514665252231596No Hit
TCACTTGGGAGAGAAGGGACGGATTTGTAAACCTCAGAGTGAGGTTCTGCCT3410.11447524346970771No Hit
TCACTTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA3410.11447524346970771No Hit
TCACTTGGGATTAACACCTTGCTACTCTGGGTCTCATCTCTCTGGCACATTT3370.1131324253644912No Hit
TCACTTGGGGAGTGCGGTGCTGTGAGAAGTATCGGTGGGAGCTACGCTGACT3370.1131324253644912No Hit
TCACTTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT3360.11279672083818706No Hit
TCACTTGGGGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTTCAACCGG3340.1121253117855788No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC3340.1121253117855788No Hit
TCACTTGGGGAGAGACCCTGTATCAAAAAAAGAGAGAGAGAGAGAGAGGGGA3340.1121253117855788No Hit
TCACTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC3270.1097753801014499No Hit
TCACTTGGGCCTGAGGTCAGGATTTCGAGACCAGCCCGGTCTTTACTAAAAA3220.10809685746992925No Hit
TCACTTGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCG3200.10742544841732102No Hit
TCACTTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA3200.10742544841732102No Hit
TCACTTGGGATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGAAATT3160.10608263031210449No Hit
TCACTTGGGAAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG3140.10541122125949623No Hit
TCACTTGGGACTTTGCCTGGCGCTGTGCGCAAGGTCTGAAACCTTATTGCTA3130.10507551673319211No Hit
TCACTTGGGGGAGAGGAGGGCGGGGTGTCGCTCCCTTTCGCTGATGCAAGAG3130.10507551673319211No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC3090.10373269862797561No Hit
TCACTTGGGGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCATCACC3070.10306128957536735No Hit
TCACTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG3060.10272558504906323No Hit
TCACTTGGGATTTGGGGTTTGTTTGATCTGTATTTATTGCATCCTGGCCATA3050.10238988052275909No Hit
TCACTTGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAA3050.10238988052275909No Hit
TCACTTGGGGCGCCTGCTCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGCA3040.10205417599645497No Hit
TCACTTGGGGTGCAGTCCTTCACTTGGGATTCTAAAAAAGGGAGATAAAACC3040.10205417599645497No Hit
TCACTTGGGAGTGCCATCCAGGAGAAACCCCCCTGGCGGCAAGTCCAGCCTG3030.10171847147015083No Hit
TCACTTGGGAGGGCGGACGCTGGTCCGGTCTCTCCTAGGCGTCCTACAGAGC3020.10138276694384671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA453.092282E-1046.00000419
GTTTGAA351.01616024E-746.00000413
AGGTATG453.092282E-1046.00000430
TATCAAA700.046.00000421
CAGGTAG351.01616024E-746.00000419
AACAAAT351.01616024E-746.00000411
TTTACTA351.01616024E-746.00000442
CATGATT453.092282E-1046.00000432
CGCATGA351.01616024E-746.00000425
GATTTCG351.01616024E-746.00000421
GATTTAT453.092282E-1046.00000435
CAATTAT453.092282E-1046.00000444
GTAGGGT351.01616024E-746.00000420
CATTCGG351.01616024E-746.00000415
TTATCAC453.092282E-1046.00000438
CGTTATT453.092282E-1046.00000433
TAACCTG351.01616024E-746.00000436
ACTAGAT453.092282E-1046.00000418
TGATTTA453.092282E-1046.00000434
GTATGGG453.092282E-1046.00000432
Produced by FastQC (version 0.11.5)