FastQCFastQC Report
Fri 17 Jun 2016
SRR1527472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421657
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAA25960.6156662880018593No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG25570.6064170641066079No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG23390.5547162741280235No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA17300.4102860856098677No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT15290.3626170086112646No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC15230.3611940510889183No Hit
TCACTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT14800.350996188845436No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG13830.32799170890083645No Hit
TCACTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG10910.2587411094799802No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC10700.2537607581517679No Hit
TCACTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA10140.2404798212765352No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT10090.23929402334124655No Hit
TCACTTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA10080.23905686375418883No Hit
TCACTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10060.23858254458007339No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC9710.23028195903305296No Hit
TCACTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC9180.2177125009189934No Hit
TCACTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG8680.20585452156610703No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG8660.2053802023919916No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC8150.19328506345204752No Hit
TCACTTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA8020.19020198882029707No Hit
TCACTTGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGT7840.18593311625325798No Hit
TCACTTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG7430.17620957318389116No Hit
TCACTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7290.172889338965083No Hit
TCACTTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG7240.17170354102979435No Hit
TCACTTGGGAGTGCCATCCAGGAGGAACCCCCCTGGCGGCAAGTCCAGCCTG7160.16980626433333254No Hit
TCACTTGGGCCGGGCATTCGGCAGTTCCTGCTCACTAACCTGGTAGGTGTGG7030.1667231897015821No Hit
TCACTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG6830.16197999796042753No Hit
TCACTTGGGGGCTGAAGATGAGGAGGGCTACCGCAAACTCATTGACCAGAAG6760.16031988085102344No Hit
TCACTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG6350.15059633778165665No Hit
TCACTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC6100.14466734810521348No Hit
TCACTTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT6030.1430072309958094No Hit
TCACTTGGGGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAAC5530.13114925164292304No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT5430.12877765577234576No Hit
TCACTTGGGGGTGGCCACGTGGCGCACGCGCAGTGCGGAATTGTAGGTCTAC5330.1264060599017685No Hit
TCACTTGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT5260.12474594279236441No Hit
TCACTTGGGGTGCTTCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG5230.12403446403119123No Hit
TCACTTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGG5130.12166286816061396No Hit
TCACTTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA5120.12142570857355624No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC5110.1211885489864985No Hit
TCACTTGGGATTAACACCTTGCTACTCTGGGTCTCATCTCTCTGGCACATTT4980.11810547435474805No Hit
TCACTTGGGAGGCGGTGGTGGCGCACTCCTTTGATCCCAGCACTTGGGAGGC4970.11786831476769033No Hit
TCACTTGGCGGATGTGAGGTGTGGTTGTCCAGCTTTTGCCAAAGCGGAAACG4950.11739399559357487No Hit
TCACTTGGGGCCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACG4920.11668251683240169No Hit
TCACTTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT4910.11644535724534397No Hit
TCACTTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA4910.11644535724534397No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC4890.11597103807122851No Hit
TCACTTGGGATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGAAATT4880.11573387848417079No Hit
TCACTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG4880.11573387848417079No Hit
TCACTTGGGGAGTGCGGTGCTGTGAGAAGTATCGGTGGGAGCTACGCTGACT4880.11573387848417079No Hit
TCACTTGGGGGAGACGGCTCAGGGTAGTCGGAGTTTGGGAGCTTTGCGCGTG4750.11265080385242034No Hit
TCACTTGGGGTGCAGTCCTTCACTTGGGATTCTAAAAAAGGGAGATAAAACC4660.11051636756890079No Hit
TCACTTGGGGCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA4640.11004204839478533No Hit
TCACTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC4570.10838193128538125No Hit
TCACTTGGGGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTTCAACCGG4550.10790761211126579No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC4540.10767045252420807No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGGAG4480.1062474950018617No Hit
TCACTTGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCG4470.10601033541480398No Hit
TCACTTGGGGAGGATGAATCTGAAGATTCCTGAAGGCCAGAATCCTTAGCTG4470.10601033541480398No Hit
TCACTTGGGAAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG4450.10553601624068852No Hit
TCACTTGGGATTTGGGGTTTGTTTGATCTGTATTTATTGCATCCTGGCCATA4410.10458737789245762No Hit
TCACTTGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT4320.10245294160893807No Hit
TCACTTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA4320.10245294160893807No Hit
TCACTTGGGGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCATCACC4310.10221578202188034No Hit
TCACTTGGGAGGGCGGACGCTGGTCCGGTCTCTCCTAGGCGTCCTACAGAGC4280.10150430326070715No Hit
TCACTTGGGGAGAGACCCTGTATCAAAAAAAGAGAGAGAGAGAGAGAGGGGA4280.10150430326070715No Hit
TCACTTGGGACTTTGCCTGGCGCTGTGCGCAAGGTCTGAAACCTTATTGCTA4270.10126714367364943No Hit
TCACTTGGGGGATTTGGAGGACTGAAGGAAGATGGGTCAAAGCCAGAGTGGT4250.10079282449953397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTA850.046.00000442
AGATAAA551.8189894E-1246.00000443
AATCTGA850.046.00000417
CATAATT551.8189894E-1246.00000412
TTACACA551.8189894E-1246.00000414
AGGTATG501.6370905E-1146.030
AACCGTT453.092282E-1046.022
TGATATT206.306888E-446.024
CAGAACC405.5970304E-946.019
AATCCTC206.306888E-446.014
CGGAAAT253.41345E-546.033
AACCGAA253.41345E-546.020
AGCCGAT206.306888E-446.025
ACAAATG206.306888E-446.045
GATACAG501.6370905E-1146.016
CAAACTC800.046.034
CAAACGG206.306888E-446.019
TACCCCC253.41345E-546.018
AATCATC206.306888E-446.028
CATATGT206.306888E-446.017
Produced by FastQC (version 0.11.5)