FastQCFastQC Report
Fri 17 Jun 2016
SRR1527471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4693
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGG2415.1353079053910085No Hit
ACGAGCGGGATAAATACTGGGCTTACACTGCTCTTACCAACATCTGTGAC2054.3682079693160025No Hit
ACGAGCGGGACCCACCATCACACCCACAACAACTGTTGTCCACCCATCAC1944.133816322181973No Hit
ACGAGCGGGGTATCTGTGTGAATATCCTGCCCTCTTCCAAGCCCTGCCTG1643.494566375452802No Hit
ACGAGCGGGAGAGACAATGGCAGGGCTGTGGAAGACCTTTGTATTGGTGG1352.87662476028127No Hit
ACGAGCATCATCTTGATTAAGCTCATTAGGGTTAGCCTCGGTACGGTCAG1352.87662476028127No Hit
ACGAGCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG1352.87662476028127No Hit
ACGAGCGGGGTCAGAGATCTTTGCAACCTCTGTTGGCTTCATCTCCAGCA1202.5569997869166845No Hit
ACGAGCGGGATTGGCCAAAAAAAGACCCTACTGCTTCTAATCTGCTTTGA1112.365224802897933No Hit
ACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAA952.0242914979757085No Hit
ACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGAC931.9816748348604303No Hit
ACGAGCGGGTGTACACACACACACACAAAAGTAAGTACAGGAGGTAATGC851.811208182399318No Hit
ACGAGCGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGC791.683358193053484No Hit
ACGAGCGGGTGTCCAGCTCTCTGCAACCCCACGGGCTGTAGCCTGCCAGG781.662049861495845No Hit
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA781.662049861495845No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC751.5981248668229278No Hit
ACGAGCGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTTAA751.5981248668229278No Hit
ACGAGCGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAG751.5981248668229278No Hit
ACGAGCGGGAAATGGTGGAAGCACAGTTGGCAACCTTTATGAAGAAAGAG701.4915832090347325No Hit
ACGAGCGGGAGCGCTGATGAGCTTCCTCACAGGTCAGCTCTTCCAGACAG691.4702748774770935No Hit
ACGAGCGGGAGGGCCAGGTTCTGGGAGAGGGGAGGGATAGCCTTCTTGTT681.4489665459194545No Hit
ACGAGCGGGGGTCGGCAGACAAGGAGAAGAACAAAGGTTCTCTCAAGAGG671.4276582143618155No Hit
ACGAGCGGGACATTTAGCTTGGGAAGATGAGAGCAGCAGGGAATTGTTTT631.3424248881312595No Hit
ACGAGCGGGGGTTTTCGTTGGCCACGCTCTGATGGCGGTCACTGCTGGAG631.3424248881312595No Hit
ACGAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC631.3424248881312595No Hit
ACGAGCGGGAGTGGCTCATGCCTGTAACCCTAGCATTTGAAAGGCCAATG601.2784998934583423No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA571.214574898785425No Hit
ACGAGCGGGGTGAAATCAGACGCTATCAGAAGTCCACTGAACTTCTGATC511.0867249094395908No Hit
ACGAGCGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCT481.0227999147666738No Hit
ACGAGCGGGGGCGCGATTGTTCCAGAGTCAGTACCATGAGCCACAATTCT460.9801832516513956No Hit
ACGAGCGGGACTCTCTGCTCTTCCTCCTCCAGCACACATCAGACCTAGTA460.9801832516513956No Hit
ACGAGCGGGGGGTTGGTATTTCCTTTGTAAAGGAAGAAATGTCTTTATTG420.8949499254208395No Hit
ACGAGCGGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTC410.8736415938632005No Hit
ACGAGCGGGCGTCCAGATTATCACTCAGATAACCCTTCCTCAGACAGTTT390.8310249307479225No Hit
ACGAGCGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGAC380.8097165991902834No Hit
ACGAGCGGGGTGGGAAAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.7884082676326444No Hit
ACGAGCGGGATCACTGGTTCCCAGAAAAGCCGTGCAAGGGATCAGGTTAC360.7670999360750054No Hit
ACGAGCGGGGCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGG360.7670999360750054No Hit
ACGAGCGGGGCTTTGCTAAGGGTCACATTGAAGTAACGCTACTCCGGGAC350.7457916045173663No Hit
ACGAGCGGGGCAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGA290.6179416151715321No Hit
ACGAGCGGGGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTC280.596633283613893No Hit
ACGAGCGGGAACTCCTGGTTGGCAGTTTTGCACATTCATACAAAACAAAA280.596633283613893No Hit
ACGAGCGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGA270.575324952056254No Hit
ACGAGCGGGAGTAATTCTGGAACTATAGGAAAAGTGTTCCCAACTGCCGT270.575324952056254No Hit
ACGAGCGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCC270.575324952056254No Hit
ACGAGCGGGACTAATGGATCCCTGGACTCTGCCAATATTGGATAATCTTT270.575324952056254No Hit
ACGAGCGGGACTGCCCGGCCACCACATGCATCCTTGATGGCAATGGATCC260.554016620498615No Hit
ACGAGCGGGAACAGCACGGAGCCCAAAGTCCCCAACAGCGCCAGGGCTGC260.554016620498615No Hit
ACGAGCGGGAAGAAAAAGCTGAGTGACCAGGCTTACTGCCAAGTAGAAGA250.5327082889409759No Hit
ACGAGCGGGGTGAATTCACCGAGCACTGACCTAGACGTTTGTCAGCCTAT250.5327082889409759No Hit
ACGAGCGGGAAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCA250.5327082889409759No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.44747496271041975No Hit
ACGAGCGGGTATTTTTTCACAACTAGAGTCTTCAGAATTAGCTTTTGCTT200.42616663115278075No Hit
ACGAGCGGGGGAATAAACTGCTGATAGAAGAAAAAAAAAAAAAAAAAAAA200.42616663115278075No Hit
ACGAGCGGGACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.3835499680375027No Hit
ACGAGCGGGGTTTTTGTTTTTTTTGTTTTGTTTTTTCAAGACAGGGTTTC150.31962497336458556No Hit
ACGAGCGGGAGAGACAATGGCAGGGCTGTGGGAGACCTTTGTATTGGTGG150.31962497336458556No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.31962497336458556No Hit
ACGAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC150.31962497336458556No Hit
ACGAGCGGGGGTGGTTAGGATTTGAACTCAGGACCTTCCGAAGAGCAGTC110.2343916471340294No Hit
ACGAGCGGGAGCAGTGATATCAATGCAGAGTACAGTGCTACGAGCGGGGG110.2343916471340294No Hit
ACGAGCGGGTTGAAAGGCAGCAGAATCCAACCGAGAACTAAGCCCAAAAA110.2343916471340294No Hit
ACGAGCTCTTCCGATCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGG100.21308331557639038Illumina Single End Adapter 2 (97% over 34bp)
ACGAGCGGGGCTGTGCTGTACTATGTGTGAGGTTGACCAGAAAGTCTAAG100.21308331557639038No Hit
ACGAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC100.21308331557639038No Hit
ACGAGCTCTTCCGATCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA60.12784998934583422Illumina Single End Adapter 2 (97% over 34bp)
ACGAGCGGGACCCACCATCACACCCACAACAACTGTTGTCCACACATCAC60.12784998934583422No Hit
ACGAGCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGGAAGG50.10654165778819519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTC253.7945952E-544.027
GCGGGGT405.671609E-944.05
GCGGGGG600.044.05
GCGGGGC351.0752592E-744.05
GCGGGGA750.044.05
GCGGGAT452.9649527E-1044.05
GCGGGAG750.044.05
GCGGGAC351.0752592E-744.05
TAGCGCC207.074439E-444.044
GGAGAGA207.074439E-444.08
CAATGGC207.074439E-444.015
TGGCAGG207.074439E-444.018
CCAACAT207.074439E-444.037
TAAATAC253.7945952E-544.011
GAGTGGC207.074439E-444.09
CGGGGTG207.074439E-444.06
TTACCAA207.074439E-444.034
CGGGGGT207.074439E-444.06
TGCAACC253.7945952E-544.022
CGGGGGG253.7945952E-544.06
Produced by FastQC (version 0.11.5)