FastQCFastQC Report
Fri 17 Jun 2016
SRR1527470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3811
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC2296.008921542902126No Hit
ACGAGCGGGATAAATACTGGGCTTACACTGCTCTTACCAACATCTGTGACTC2065.405405405405405No Hit
ACGAGCGGGACCCACCATCACACCCACAACAACTGTTGTCCACCCATCACAG1413.6998163211755446No Hit
ACGAGCGGGAGAGACAATGGCAGGGCTGTGGAAGACCTTTGTATTGGTGGTG1393.6473366570453947No Hit
ACGAGCGGGGTATCTGTGTGAATATCCTGCCCTCTTCCAAGCCCTGCCTGCA1203.148779847808974No Hit
ACGAGCGGGGTCAGAGATCTTTGCAACCTCTGTTGGCTTCATCTCCAGCATA1012.650223038572553No Hit
ACGAGCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA982.5715035423773287No Hit
ACGAGCATCATCTTGATTAAGCTCATTAGGGTTAGCCTCGGTACGGTCAGGC932.4403043820519548No Hit
ACGAGCGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTTAAGC842.204145893466282No Hit
ACGAGCGGGATTGGCCAAAAAAAGACCCTACTGCTTCTAATCTGCTTTGAAT782.0467069010758334No Hit
ACGAGCGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCA721.8892679086853843No Hit
ACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAA701.8367882445552346No Hit
ACGAGCGGGTGTCCAGCTCTCTGCAACCCCACGGGCTGTAGCCTGCCAGGCT671.7580687483600106No Hit
ACGAGCGGGGGTTTTCGTTGGCCACGCTCTGATGGCGGTCACTGCTGGAGCT671.7580687483600106No Hit
ACGAGCGGGAGTGGCTCATGCCTGTAACCCTAGCATTTGAAAGGCCAATGCA641.6793492521647861No Hit
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA621.6268695880346364No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT611.600629755969562No Hit
ACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCA611.600629755969562No Hit
ACGAGCGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCG601.574389923904487No Hit
ACGAGCGGGAGCGCTGATGAGCTTCCTCACAGGTCAGCTCTTCCAGACAGCC591.5481500918394122No Hit
ACGAGCGGGGGTCGGCAGACAAGGAGAAGAACAAAGGTTCTCTCAAGAGGAA591.5481500918394122No Hit
ACGAGCGGGACATTTAGCTTGGGAAGATGAGAGCAGCAGGGAATTGTTTTTT591.5481500918394122No Hit
ACGAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG561.4694305956441878No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA561.4694305956441878No Hit
ACGAGCGGGTGTACACACACACACACAAAAGTAAGTACAGGAGGTAATGCAT551.443190763579113No Hit
ACGAGCGGGAAATGGTGGAAGCACAGTTGGCAACCTTTATGAAGAAAGAGAA541.4169509315140383No Hit
ACGAGCGGGAGGGCCAGGTTCTGGGAGAGGGGAGGGATAGCCTTCTTGTTTT541.4169509315140383No Hit
ACGAGCGGGGAAAGCCTAACCAAAACAACCTGAACTATTTAAAGCAAGTAAC531.3907110994489635No Hit
ACGAGCGGGGTGAAATCAGACGCTATCAGAAGTCCACTGAACTTCTGATCCG370.9708737864077669No Hit
ACGAGCGGGCGTCCAGATTATCACTCAGATAACCCTTCCTCAGACAGTTTCC340.8921542902125426No Hit
ACGAGCGGGGCTTTGCTAAGGGTCACATTGAAGTAACGCTACTCCGGGACAA330.8659144581474678No Hit
ACGAGCGGGGGGTTGGTATTTCCTTTGTAAAGGAAGAAATGTCTTTATTGTA320.8396746260823931No Hit
ACGAGCGGGAACAGCACGGAGCCCAAAGTCCCCAACAGCGCCAGGGCTGCCA300.7871949619522435No Hit
ACGAGCGGGATCACTGGTTCCCAGAAAAGCCGTGCAAGGGATCAGGTTACCG300.7871949619522435No Hit
ACGAGCGGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTC280.7347152978220939No Hit
ACGAGCGGGACTCTCTGCTCTTCCTCCTCCAGCACACATCAGACCTAGTAGC280.7347152978220939No Hit
ACGAGCGGGACTGCCCGGCCACCACATGCATCCTTGATGGCAATGGATCCTC270.7084754657570191No Hit
ACGAGCGGGGTGGGAAAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.6822356336919444No Hit
ACGAGCGGGGCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGGGT260.6822356336919444No Hit
ACGAGCGGGGCAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGATC260.6822356336919444No Hit
ACGAGCGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTCC250.6559958016268695No Hit
ACGAGCGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA230.6035161374967201No Hit
ACGAGCGGGAGTAATTCTGGAACTATAGGAAAAGTGTTCCCAACTGCCGTGA230.6035161374967201No Hit
ACGAGCGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGACCT220.5772763054316452No Hit
ACGAGCGGGGTGAATTCACCGAGCACTGACCTAGACGTTTGTCAGCCTATGA210.5510364733665705No Hit
ACGAGCGGGAAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC190.49855680923642093No Hit
ACGAGCGGGAAGAAAAAGCTGAGTGACCAGGCTTACTGCCAAGTAGAAGACT190.49855680923642093No Hit
ACGAGCGGGACTAATGGATCCCTGGACTCTGCCAATATTGGATAATCTTTGC190.49855680923642093No Hit
ACGAGCGGGACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.4723169771713461No Hit
ACGAGCGGGGGTGGTTAGGATTTGAACTCAGGACCTTCCGAAGAGCAGTCAG180.4723169771713461No Hit
ACGAGCGGGAACTCCTGGTTGGCAGTTTTGCACATTCATACAAAACAAAATT180.4723169771713461No Hit
ACGAGCGGGTATTTTTTCACAACTAGAGTCTTCAGAATTAGCTTTTGCTTTG180.4723169771713461No Hit
ACGAGCGGGGGCGCGATTGTTCCAGAGTCAGTACCATGAGCCACAATTCTCA170.4460771451062713No Hit
ACGAGCGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCCTG160.41983731304119654No Hit
ACGAGCGGGAGCAGTGATATCAATGCAGAGTACAGTGCTACGAGCGGGGGAT150.39359748097612174No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3411178168459722No Hit
ACGAGCGGGGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTCCT120.3148779847808974No Hit
ACGAGCGGGGGAATAAACTGCTGATAGAAGAAAAAAAAAAAAAAAAAAAAAA120.3148779847808974No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.26239832065074786No Hit
ACGAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT80.20991865652059827No Hit
ACGAGCGGGGCTGTGCTGTACTATGTGTGAGGTTGACCAGAAAGTCTAAGAA80.20991865652059827No Hit
ACGAGCGGGGTTTTTGTTTTTTTTGTTTTGTTTTTTCAAGACAGGGTTTCTC80.20991865652059827No Hit
ACGAGCGGGTTGAAAGGCAGCAGAATCCAACCGAGAACTAAGCCCAAAAAAA70.18367882445552347No Hit
ACGAGCGGGGTGCAGTGCTACGAGCGGGGAGAAGCTGCATGAGAACAACCCA50.13119916032537393No Hit
ACGAGCGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAAC50.13119916032537393No Hit
ACGAGCGGGGTGTGTGTGTGTGTGTGTGTGTGGTTGTGTGCACATGAGTGTG50.13119916032537393No Hit
ACGAGCTCTTCCGATCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAT50.13119916032537393Illumina Single End Adapter 2 (97% over 34bp)
ACGAGCGGGGTCAGAGATCTTTGCAACCTCTGTTGGAGTCATGTGGGTTTGG50.13119916032537393No Hit
ACGAGCGGGGTCGCTAGATCTTCTAATCCATACAAAAAAAAAAAAAAAAAAA40.10495932826029913No Hit
ACGAGCGGGGAACATCTGTGACTCCTTAGCCTTGAAGAGCAAGAAGATGGCC40.10495932826029913No Hit
ACGAGCGGGGGGAATCCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG40.10495932826029913No Hit
ACGAGCGGGTGTACACACACACACAAAAGTAAGTACAGGAGGTAATGCATAT40.10495932826029913No Hit
ACGAGCTCTTCCGATCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGATC40.10495932826029913Illumina Single End Adapter 2 (97% over 34bp)
ACGAGCGGGATTGGCCAAAAAAAGACCCTACTGCTTCTAATCTGCCTTGAAT40.10495932826029913No Hit
ACGAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA40.10495932826029913No Hit
ACGAGCGGGGGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTTAAGCAC40.10495932826029913No Hit
ACGAGCGGGGTTTTTGTTTTTTTGTTTTGTTTTTTCAAGACAGGGTTTCTCT40.10495932826029913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT205.54699E-446.0000045
GCGGGAT403.4979166E-946.0000045
GCGGGAG403.4979166E-946.0000045
GCGGGAC403.4979166E-946.0000045
TGCAACC205.54699E-446.00000422
CGGGGGG205.54699E-446.0000046
CGGGGGA205.54699E-446.0000046
CGGGGAT403.4979166E-946.0000046
GTTTTTT205.54699E-446.00000446
AGCGGGG1450.046.0000044
CGGGACC205.54699E-446.0000046
GGGGATC205.54699E-446.0000047
GCGGGGG550.046.05
GCGGGGA600.046.05
GAGCGGG3250.046.03
ACGAGCG3450.046.01
ACGAGCA301.4163434E-646.01
AGCGGGA1550.046.04
CGGGAAA301.4163434E-646.06
GCGGGAA357.071503E-845.9999965
Produced by FastQC (version 0.11.5)