FastQCFastQC Report
Fri 17 Jun 2016
SRR1527469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4668
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC2455.248500428449015No Hit
ACGAGCGGGATAAATACTGGGCTTACACTGCTCTTACCAACATCTGTGACTC2194.691516709511568No Hit
ACGAGCGGGACCCACCATCACACCCACAACAACTGTTGTCCACCCATCACAG1974.220222793487575No Hit
ACGAGCGGGAGAGACAATGGCAGGGCTGTGGAAGACCTTTGTATTGGTGGTG1924.113110539845758No Hit
ACGAGCGGGGTATCTGTGTGAATATCCTGCCCTCTTCCAAGCCCTGCCTGCA1663.556126820908312No Hit
ACGAGCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA1583.384747215081405No Hit
ACGAGCGGGGTCAGAGATCTTTGCAACCTCTGTTGGCTTCATCTCCAGCATA1332.849185946872322No Hit
ACGAGCGGGATTGGCCAAAAAAAGACCCTACTGCTTCTAATCTGCTTTGAAT1062.2707797772065126No Hit
ACGAGCGGGAGTGGCTCATGCCTGTAACCCTAGCATTTGAAAGGCCAATGCA1062.2707797772065126No Hit
ACGAGCGGGGGTCGGCAGACAAGGAGAAGAACAAAGGTTCTCTCAAGAGGAA1002.1422450728363325No Hit
ACGAGCGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTTAAGC931.992287917737789No Hit
ACGAGCGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCA871.8637532133676094No Hit
ACGAGCGGGAGCGCTGATGAGCTTCCTCACAGGTCAGCTCTTCCAGACAGCC861.8423307626392458No Hit
ACGAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG851.8209083119108824No Hit
ACGAGCGGGTGTCCAGCTCTCTGCAACCCCACGGGCTGTAGCCTGCCAGGCT811.7352185089974295No Hit
ACGAGCGGGAGGGCCAGGTTCTGGGAGAGGGGAGGGATAGCCTTCTTGTTTT791.6923736075407025No Hit
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA771.649528706083976No Hit
ACGAGCGGGAAATGGTGGAAGCACAGTTGGCAACCTTTATGAAGAAAGAGAA741.5852613538988862No Hit
ACGAGCGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCG731.5638389031705229No Hit
ACGAGCGGGTGTACACACACACACACAAAAGTAAGTACAGGAGGTAATGCAT721.5424164524421593No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT721.5424164524421593No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA691.4781491002570695No Hit
ACGAGCGGGACATTTAGCTTGGGAAGATGAGAGCAGCAGGGAATTGTTTTTT651.392459297343616No Hit
ACGAGCATCATCTTGATTAAGCTCATTAGGGTTAGCCTCGGTACGGTCAGGC641.371036846615253No Hit
ACGAGCGGGGGTTTTCGTTGGCCACGCTCTGATGGCGGTCACTGCTGGAGCT621.3281919451585262No Hit
ACGAGCGGGACTCTCTGCTCTTCCTCCTCCAGCACACATCAGACCTAGTAGC591.2639245929734362No Hit
ACGAGCGGGGGGTTGGTATTTCCTTTGTAAAGGAAGAAATGTCTTTATTGTA521.113967437874893No Hit
ACGAGCGGGCGTCCAGATTATCACTCAGATAACCCTTCCTCAGACAGTTTCC501.0711225364181662No Hit
ACGAGCGGGGGCGCGATTGTTCCAGAGTCAGTACCATGAGCCACAATTCTCA481.0282776349614395No Hit
ACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCA440.9425878320479864No Hit
ACGAGCGGGGTGAAATCAGACGCTATCAGAAGTCCACTGAACTTCTGATCCG430.9211653813196229No Hit
ACGAGCGGGATCACTGGTTCCCAGAAAAGCCGTGCAAGGGATCAGGTTACCG400.8568980291345331No Hit
ACGAGCGGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTC390.8354755784061696No Hit
ACGAGCGGGGCTTTGCTAAGGGTCACATTGAAGTAACGCTACTCCGGGACAA380.8140531276778064No Hit
ACGAGCGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGACCT370.7926306769494431No Hit
ACGAGCGGGGCAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGATC360.7712082262210797No Hit
ACGAGCGGGGTGGGAAAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.7283633247643531No Hit
ACGAGCGGGAAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC330.7069408740359897No Hit
ACGAGCGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA320.6855184233076265No Hit
ACGAGCGGGAACAGCACGGAGCCCAAAGTCCCCAACAGCGCCAGGGCTGCCA320.6855184233076265No Hit
ACGAGCGGGGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTCCT320.6855184233076265No Hit
ACGAGCGGGAGCCAGCAGCTGACCCAGCCTCCAGTCACCATGAAGACTCTCC310.6640959725792631No Hit
ACGAGCGGGACTGCCCGGCCACCACATGCATCCTTGATGGCAATGGATCCTC300.6426735218508998No Hit
ACGAGCGGGGCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGGGT300.6426735218508998No Hit
ACGAGCGGGTATTTTTTCACAACTAGAGTCTTCAGAATTAGCTTTTGCTTTG270.5784061696658097No Hit
ACGAGCGGGAACTCCTGGTTGGCAGTTTTGCACATTCATACAAAACAAAATT260.5569837189374465No Hit
ACGAGCGGGAAGAAAAAGCTGAGTGACCAGGCTTACTGCCAAGTAGAAGACT260.5569837189374465No Hit
ACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAA240.5141388174807198No Hit
ACGAGCGGGGTGAATTCACCGAGCACTGACCTAGACGTTTGTCAGCCTATGA240.5141388174807198No Hit
ACGAGCGGGAGTAATTCTGGAACTATAGGAAAAGTGTTCCCAACTGCCGTGA240.5141388174807198No Hit
ACGAGCGGGACTAATGGATCCCTGGACTCTGCCAATATTGGATAATCTTTGC240.5141388174807198No Hit
ACGAGCGGGGGAATAAACTGCTGATAGAAGAAAAAAAAAAAAAAAAAAAAAA210.4498714652956298No Hit
ACGAGCGGGAGTCTTGGGAACCATGCAGTTCCAGAGGGACGTCCCCTCCCTG200.42844901456726653No Hit
ACGAGCGGGACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.3856041131105398No Hit
ACGAGCGGGAGCAGTGATATCAATGCAGAGTACAGTGCTACGAGCGGGGGAT180.3856041131105398No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.36418166238217653No Hit
ACGAGCGGGGCTGTGCTGTACTATGTGTGAGGTTGACCAGAAAGTCTAAGAA120.2570694087403599No Hit
ACGAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT110.2356469580119966No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2356469580119966No Hit
ACGAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG100.21422450728363326No Hit
ACGAGCGGGGTTTTTGTTTTTTTTGTTTTGTTTTTTCAAGACAGGGTTTCTC100.21422450728363326No Hit
ACGAGCGGGTGTACACACACACACAAAAGTAAGTACAGGAGGTAATGCATAT90.1928020565552699No Hit
ACGAGCGGGGGTGGTTAGGATTTGAACTCAGGACCTTCCGAAGAGCAGTCAG90.1928020565552699No Hit
ACGAGCGGGTTGAAAGGCAGCAGAATCCAACCGAGAACTAAGCCCAAAAAAA80.17137960582690662No Hit
ACGAGCGGGGTGTGTGTGTGTGTGTGTGTGTGGTTGTGTGCACATGAGTGTG80.17137960582690662No Hit
ACGAGCGGGGAAAGCCTAACCAAAACAACCTGAACTATTTAAAGCAAGTAAC80.17137960582690662No Hit
ACGAGCGGGGGTTTTCGTTGGCCACGCTCTAGTGGCGAGACTCCCGGCCCAT60.12853470437017994No Hit
ACGAGCGGGGTCGCTAGATCTTCTAATCCATACAAAAAAAAAAAAAAAAAAA50.10711225364181663No Hit
ACGAGCGGGGCTTTGTCTCGGGCCCGAGTTCGTAGGCGCAGCCGGCCTAGCG50.10711225364181663No Hit
ACGAGCGGGTGTACACACACACACACAAACGTAAGTACAGGAGGTAATGCAT50.10711225364181663No Hit
ACGAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA50.10711225364181663No Hit
ACGAGCTCTTCCGATCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAT50.10711225364181663Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAAGG357.577182E-846.00000431
GGAGAAG357.577182E-846.00000423
CAAGAGG357.577182E-846.00000444
GAGAAGA357.577182E-846.00000424
GGGTCGG357.577182E-846.0000049
AACAAAG357.577182E-846.00000430
TCAAGAG357.577182E-846.00000443
AGAAGAA357.577182E-846.00000425
GTTCTCT357.577182E-846.00000437
TCGGCAG357.577182E-846.00000412
AGAGGAA357.577182E-846.00000446
AAGAGGA357.577182E-846.00000445
CTCTCAA357.577182E-846.00000440
GGTTCTC357.577182E-846.00000436
GAAGAAC357.577182E-846.00000426
AGAACAA357.577182E-846.00000428
GGGGGTC357.577182E-846.0000047
TTCTCTC357.577182E-846.00000438
GACAAGG357.577182E-846.00000418
AAAGGTT357.577182E-846.00000433
Produced by FastQC (version 0.11.5)