FastQCFastQC Report
Fri 17 Jun 2016
SRR1527468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524258
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT44780.854159593177405No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC40010.763173857146672No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC28910.5514460437418217No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC26450.5045225823926388No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24380.46503820637930177No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGA15670.2988986338787391No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG13700.2613217156438242No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC12510.23862296808060154No Hit
CGTATTGGGTTGTACATATTGTATTTTTACTATTGAACTGTACTCTAGTG11530.2199298818520652No Hit
CGTATTGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGA11310.21573347473953663No Hit
CGTATTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT10810.20619618584742627No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC10680.20371649073547757No Hit
CGTATTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC10490.2000923209564756No Hit
CGTATTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG10180.1941792018433672No Hit
CGTATTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA10090.19246248984278733No Hit
CGTATTGGGACAATGCATCGCGGTGGGAGGCAAGCTGGGCTCCCCTGCTC9800.18693086228536332No Hit
CGTATTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC9770.1863586249518367No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT8900.16976374227956464No Hit
CGTATTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT8550.16308764005508739No Hit
CGTATTGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG8170.1558393004970835No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT7970.15202438494023934No Hit
CGTATTGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTC7820.14916319827260624No Hit
CGTATTGGGGCTTCACACTACGCCGGAAGGGGATGAACCGCTTCCCGGTG7790.1485909609390796No Hit
CGTATTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC7770.14820946938339521No Hit
CGTATTGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCT7720.14725574049418416No Hit
CGTATTGGGGAAAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCT7700.14687424893849974No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG7320.13962590938049585No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG7220.1377184516020738No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG7020.13390353604522964No Hit
CGTATTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT6890.13142384093328097No Hit
CGTATTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA6520.12436624715311928No Hit
CGTATTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG6380.12169580626332835No Hit
CGTATTGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG6270.11959760270706407No Hit
CGTATTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG6260.11940685692922187No Hit
CGTATTGAGGGAGGTTGAGGCAGGAGAATGGCATGAACCCGGGAAGCGGA6010.11463821248316668No Hit
CGTATTGGGACTCTTCGTCAGTGCTATCTTCTTGTATGTCATTGTGAGCA5980.11406597514964008No Hit
CGTATTGGGGAAGAACAGTATGTGCTGTTGACCTCTGATCCTTCTCTTCA5950.11349373781611345No Hit
CGTATTGGGACTTCCGGCGGTGGCGATTTGGAGGACTGAAGGAAGATGGG5940.11330299203827124No Hit
CGTATTGGGCAGAAGCCCATCCCTAGTCTCCAAAGCCCTGCCTCTGGGCA5770.11006031381495371No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA5630.1073898729251628No Hit
CGTATTGGGGCTCGTTTTAACAATTATCTTTAGGCCTTTGAAGAGCAGCA5620.10719912714732059No Hit
CGTATTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT5620.10719912714732059No Hit
CGTATTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA5450.10395644892400306No Hit
CGTATTGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACACTGACTGC5410.10319346581263425No Hit
CGTATTGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT5290.10090451647852774No Hit
CGTATTGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAG5290.10090451647852774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTT650.044.0000041
TAACGCG650.044.00000418
TGTTATA650.044.00000413
TATAACG650.044.00000416
GTTATAA650.044.00000414
TAATCAC207.852559E-444.016
TCACGAC207.852559E-444.029
TTCTATC207.852559E-444.026
GTGTTAT207.852559E-444.09
TTAGGTC207.852559E-444.012
GAGGATA302.525072E-644.09
CAGGTAT302.525072E-644.036
GAGTGAT408.29823E-944.09
ACTATCA207.852559E-444.022
AATCCGT207.852559E-444.035
GTACCGC254.439517E-544.038
TACCCCC600.044.024
TGCCCAT302.525072E-644.015
CGAACAT254.439517E-544.037
CGTATGT207.852559E-444.012
Produced by FastQC (version 0.11.5)