FastQCFastQC Report
Fri 17 Jun 2016
SRR1527467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425253
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG36490.8580774268494286No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC33860.7962318901924266No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT24450.5749518521915189No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC21740.5112250824803117No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC19820.4660754891793826No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA11170.26266716519342603No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT10400.24456029704669927No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAG10370.24385483465137223No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT10340.24314937225604522No Hit
CGTATTGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT9540.22433704171399144No Hit
CGTATTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA9090.21375510578408619No Hit
CGTATTGGGTTGTACATATTGTATTTTTACTATTGAACTGTACTCTAGTGGC8840.20787625248969438No Hit
CGTATTGGGACAATGCATCGCGGTGGGAGGCAAGCTGGGCTCCCCTGCTCCG8710.20481924877661065No Hit
CGTATTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA8640.2031731698541809No Hit
CGTATTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT8570.20152709093175122No Hit
CGTATTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG8560.20129193679997553No Hit
CGTATTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG8500.1998810120093215No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT7850.1845959934439028No Hit
CGTATTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA7040.16554850877007335No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG6690.15731811415792482No Hit
CGTATTGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT6650.15637749763082212No Hit
CGTATTGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTTC6630.1559071893672708No Hit
CGTATTGGGGCTTCACACTACGCCGGAAGGGGATGAACCGCTTCCCGGTGCC6480.1523798773906357No Hit
CGTATTGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG6210.14603071583269256No Hit
CGTATTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC6200.14579556170091687No Hit
CGTATTGGGGAAAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCT5920.13921124601119805No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC5880.13827062948409535No Hit
CGTATTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA5580.13121600553082519No Hit
CGTATTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG5450.12815900181774145No Hit
CGTATTGGGACTTCCGGCGGTGGCGATTTGGAGGACTGAAGGAAGATGGGTC5440.12792384768596576No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT5410.12721838529063875No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5350.12580746049998473No Hit
CGTATTGAGGGAGGTTGAGGCAGGAGAATGGCATGAACCCGGGAAGCGGAGG5250.123455919182228No Hit
CGTATTGGGACTCTTCGTCAGTGCTATCTTCTTGTATGTCATTGTGAGCAAG5240.12322076505045232No Hit
CGTATTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT5170.12157468612802261No Hit
CGTATTGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC5040.11851768241493887No Hit
CGTATTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA5000.11757706588783619No Hit
CGTATTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG4990.11734191175606051No Hit
CGTATTGGGCAGAAGCCCATCCCTAGTCTCCAAAGCCCTGCCTCTGGGCAAC4980.11710675762428484No Hit
CGTATTGGGGAAGAACAGTATGTGCTGTTGACCTCTGATCCTTCTCTTCATT4900.11522552457007947No Hit
CGTATTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC4780.1124036749887714No Hit
CGTATTGGGGTTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGAT4720.11099275019811736No Hit
CGTATTGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT4670.109816979539239No Hit
CGTATTGGGAGCGTCTTGTTAAAAAAAAAAAATCAAGAAAGAATTGAGTTTT4340.1020568931906418No Hit
CGTATTGGGCAAGGACTGGGATCGGTCTGCCCCTGCTGGGTCCATCCCACCC4310.10135143079531479No Hit
CGTATTGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT4290.10088112253176344No Hit
CGTATTGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTC4280.10064596839998777No Hit
CGTATTGGGGTTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCGGT4270.10041081426821209No Hit
CGTATTGGGGCTCGTTTTAACAATTATCTTTAGGCCTTTGAAGAGCAGCAGG4260.10017566013643643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA301.8586179E-646.028
ACAACGA405.5970304E-946.039
TGTCATT600.046.037
TTTAGCG301.8586179E-646.014
TAGAGTA206.3069473E-446.013
AAACTCG253.4135E-546.043
CGGCGAA253.4135E-546.038
GATACGA206.3069473E-446.09
CCTTAAT301.8586179E-646.031
ACACGCG206.3069473E-446.011
CATATGC206.3069473E-446.033
CATATCT301.8586179E-646.045
GTCGATG206.3069473E-446.042
GTATTCT253.4135E-546.02
CACGGTT405.5970304E-946.028
TACCAAA551.8189894E-1246.039
TAAAGCG206.3069473E-446.030
CCCTAGT551.8189894E-1246.021
CCCTACT206.3069473E-446.017
AACCCCG206.3069473E-446.017
Produced by FastQC (version 0.11.5)