FastQCFastQC Report
Fri 17 Jun 2016
SRR1527466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545916
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG48640.8909795646216634No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC43610.7988408473098426No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32450.5944137925981287No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC30260.5542977307864213No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC25720.47113475333201443No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAG15840.29015452926823904No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13900.25461792656745724No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT13720.2513207160075909No Hit
CGTATTGGGTTGTACATATTGTATTTTTACTATTGAACTGTACTCTAGTGGC12090.22146264260435672No Hit
CGTATTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA11920.2183486104089274No Hit
CGTATTGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT11410.20900651382263938No Hit
CGTATTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG11350.20790744363601726No Hit
CGTATTGGGACAATGCATCGCGGTGGGAGGCAAGCTGGGCTCCCCTGCTCCG11130.20387751961840284No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10760.1970999201342331No Hit
CGTATTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA10710.196184028312048No Hit
CGTATTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT10140.18574286153913788No Hit
CGTATTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG9960.18244565097927154No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT9700.17768301350390903No Hit
CGTATTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA9100.16669231163768786No Hit
CGTATTGGGGAAAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCT9080.1663259549088138No Hit
CGTATTGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG8720.15973153378908111No Hit
CGTATTGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT8710.15954835542464407No Hit
CGTATTGGGGCTTCACACTACGCCGGAAGGGGATGAACCGCTTCCCGGTGCC8370.15332029103378542No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8340.15277075594047435No Hit
CGTATTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC8160.149473545380608No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC8040.14727540500736377No Hit
CGTATTGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTTC7980.14617633482074166No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC7630.13976509206544596No Hit
CGTATTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT7570.13866602187882385No Hit
CGTATTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA7270.13317067094571325No Hit
CGTATTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7220.13225477912352815No Hit
CGTATTGAGGGAGGTTGAGGCAGGAGAATGGCATGAACCCGGGAAGCGGAGG7040.1289575685636618No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT6600.12089772052843295No Hit
CGTATTGGGACTCTTCGTCAGTGCTATCTTCTTGTATGTCATTGTGAGCAAG6580.12053136379955891No Hit
CGTATTGGGGTTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGAT6440.11796686669744064No Hit
CGTATTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA6400.11723415323969255No Hit
CGTATTGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT6400.11723415323969255No Hit
CGTATTGGGACTTCCGGCGGTGGCGATTTGGAGGACTGAAGGAAGATGGGTC6370.1166846181463815No Hit
CGTATTGGGGCTCGTTTTAACAATTATCTTTAGGCCTTTGAAGAGCAGCAGG6340.11613508305307044No Hit
CGTATTGGGCAGAAGCCCATCCCTAGTCTCCAAAGCCCTGCCTCTGGGCAAC6270.1148528345020113No Hit
CGTATTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG6090.11155562394214494No Hit
CGTATTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC5950.10899112684002667No Hit
CGTATTGGGGAAGAACAGTATGTGCTGTTGACCTCTGATCCTTCTCTTCATT5800.10624345137347138No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC5760.1055107379157233No Hit
CGTATTGGGAGCGTCTTGTTAAAAAAAAAAAATCAAGAAAGAATTGAGTTTT5750.10532755955128628No Hit
CGTATTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA5720.10477802445797521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCA351.0182521E-746.00000427
CGCGGAA351.0182521E-746.00000434
CCCTAGT700.046.00000421
GGACTAA551.8189894E-1246.0000048
ATCCGTA351.0182521E-746.00000442
TAGCATA351.0182521E-746.00000430
TTATAAT351.0182521E-746.00000435
CCGTAAT351.0182521E-746.00000444
TCGTGGC351.0182521E-746.00000421
CACTAGG351.0182521E-746.00000422
ATCCCTA700.046.00000419
GTACTCT1400.046.00000440
TACTCTA1400.046.00000441
TTAACCT351.0182521E-746.00000439
AATAACG351.0182521E-746.00000411
TGTCATT900.046.037
ACTATTC206.30853E-446.022
CTATGGG253.4147815E-546.09
TCTGATA206.30853E-446.024
GTACCTC253.4147815E-546.020
Produced by FastQC (version 0.11.5)