##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545916
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.725705053524717	31.0	31.0	33.0	30.0	33.0
2	31.095569648077728	31.0	31.0	33.0	30.0	34.0
3	27.09904270986745	28.0	25.0	30.0	16.0	31.0
4	33.40660834267543	35.0	33.0	35.0	30.0	35.0
5	34.478350149107186	35.0	35.0	35.0	33.0	37.0
6	35.57388499329567	35.0	35.0	37.0	35.0	37.0
7	36.042283061862996	37.0	35.0	37.0	35.0	37.0
8	36.43674484719261	37.0	36.0	37.0	35.0	37.0
9	38.60827306765143	39.0	39.0	39.0	37.0	39.0
10	37.211239458085124	39.0	37.0	39.0	34.0	39.0
11	37.070334996592884	39.0	37.0	39.0	34.0	39.0
12	36.932802848789926	39.0	37.0	39.0	33.0	39.0
13	36.90864711787162	39.0	37.0	39.0	33.0	39.0
14	37.74863715296859	40.0	37.0	40.0	33.0	40.0
15	37.80946519244719	40.0	37.0	40.0	33.0	40.0
16	37.863993727972804	40.0	37.0	40.0	33.0	40.0
17	37.84313154404707	40.0	37.0	40.0	33.0	40.0
18	37.7561016713194	40.0	37.0	40.0	33.0	40.0
19	37.63903787395863	40.0	37.0	40.0	33.0	40.0
20	37.54485854966698	39.0	37.0	40.0	33.0	40.0
21	37.59217718476835	39.0	37.0	40.0	33.0	40.0
22	37.55976743674851	39.0	37.0	40.0	33.0	40.0
23	37.475587819371476	39.0	37.0	40.0	33.0	40.0
24	37.43468225880905	39.0	37.0	40.0	33.0	40.0
25	37.34906652305483	39.0	36.0	40.0	33.0	40.0
26	37.215276709237315	39.0	36.0	40.0	32.0	40.0
27	37.14911634756996	39.0	36.0	40.0	32.0	40.0
28	36.965602766726015	39.0	36.0	40.0	32.0	40.0
29	36.944766227771304	39.0	36.0	40.0	32.0	40.0
30	36.850392734413354	39.0	35.0	40.0	32.0	40.0
31	36.68871035104302	39.0	35.0	40.0	31.0	40.0
32	36.52696568702877	38.0	35.0	40.0	31.0	40.0
33	36.321441393914085	38.0	35.0	40.0	31.0	40.0
34	36.07602268480865	38.0	35.0	40.0	30.0	40.0
35	35.883896057268885	38.0	35.0	40.0	30.0	40.0
36	35.78401072692502	38.0	34.0	40.0	30.0	40.0
37	35.541554011972536	38.0	34.0	40.0	29.0	40.0
38	35.42229756958946	38.0	34.0	40.0	28.0	40.0
39	35.186587313799194	38.0	34.0	40.0	28.0	40.0
40	35.50423325200214	38.0	34.0	40.0	29.0	40.0
41	35.59412620256596	38.0	34.0	40.0	29.0	40.0
42	35.620044109350154	38.0	35.0	40.0	30.0	40.0
43	35.520001245612875	38.0	34.0	40.0	29.0	40.0
44	35.402805559829716	37.0	34.0	40.0	29.0	40.0
45	35.34139318136856	37.0	34.0	40.0	29.0	40.0
46	35.00619509228526	37.0	34.0	40.0	28.0	40.0
47	34.91003744165769	36.0	34.0	40.0	28.0	40.0
48	34.753872390624196	36.0	34.0	40.0	28.0	40.0
49	34.51135889037874	36.0	33.0	39.0	27.0	40.0
50	34.30066347203599	35.0	33.0	39.0	27.0	40.0
51	34.144049634009626	35.0	33.0	39.0	27.0	40.0
52	33.62082994453359	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	10.0
16	30.0
17	55.0
18	124.0
19	242.0
20	359.0
21	608.0
22	957.0
23	1322.0
24	1978.0
25	2872.0
26	4111.0
27	5572.0
28	7230.0
29	8804.0
30	10773.0
31	14118.0
32	19219.0
33	27745.0
34	42293.0
35	50060.0
36	82745.0
37	120350.0
38	139693.0
39	4642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57026355703074	0.29931344749009003	0.08609383128539921	0.04432916419375875
8	99.14968603228336	0.7587247854981353	0.06118157372196455	0.030407608496545255
9	98.15301255138154	0.8631364532272364	0.34162764967504156	0.6422233457161908
10	59.296485173543175	27.794202771122293	4.752013130225163	8.157298925109357
11	32.24177345965313	26.295254214934165	19.97230343129712	21.49066889411558
12	25.90673290396325	20.259160750005496	29.203577107100724	24.63052923893053
13	23.13029843419134	18.99101693300801	29.965232746429855	27.9134518863708
14	24.610745975571334	18.731270012236315	30.16837755259051	26.489606459601845
15	24.8708592530719	18.473171696744554	28.789594003473063	27.866375046710484
16	29.927864360084698	17.810066017482544	24.921782838385393	27.340286784047365
17	29.71702606261769	20.301291773826012	21.162230086680005	28.819452076876296
18	30.490588295635224	19.461968507975584	22.98540434792166	27.06203884846753
19	29.935008316297747	18.42664439217755	22.437884216619405	29.200463074905297
20	28.600553931374055	21.901354787183376	22.91323207233347	26.584859209109098
21	31.843726873731487	18.941375596245578	23.308714161153	25.906183368869932
22	32.22968368760029	18.97251591819987	20.95780303196829	27.83999736223155
23	31.124751793316186	21.47015291729863	21.716527817466424	25.688567471918756
24	30.491504187457412	20.103642318598467	22.33933425655229	27.065519237391833
25	29.216949127704627	20.88819525348222	21.447988335201753	28.4468672836114
26	29.60235640648012	20.5907135896365	20.9068794466548	28.900050557228585
27	28.697638464525678	19.04432183705918	22.429458011855306	29.828581686559836
28	31.108265740516856	20.798804211636956	21.10214758314466	26.990782464701528
29	31.02510276306245	20.594926692018554	21.10892518262883	27.27104536229017
30	30.67266758988562	20.556825592215652	21.3591468284498	27.411359989448925
31	32.91422123550144	21.282395093750686	19.808541973490428	25.994841697257453
32	35.84415917467156	21.885967804570665	18.605060119139207	23.664812901618564
33	31.85288579195334	21.848233061496643	20.480440214245416	25.818440932304604
34	32.063907267784785	21.55020186255761	22.311307966793425	24.07458290286418
35	31.80196220663985	24.366752394141223	20.81382483752079	23.017460561698137
36	32.698437122194626	22.695432997017857	19.32018845390133	25.28594142688619
37	31.178423054096232	24.30355585841045	20.425669883278747	24.09235120421457
38	33.71397797463346	23.39792202463383	19.991170802834134	22.896929197898576
39	29.63111540969673	22.110361301006016	20.999934055788803	27.258589233508452
40	29.73442800723921	23.718117805669735	21.951179302310244	24.596274884780808
41	27.995882150367457	21.986715905011028	21.727701697697082	28.289700246924436
42	28.74599755273705	20.650246558078532	23.440419405183217	27.1633364840012
43	27.294492192938108	20.25641307453894	25.892078634808286	26.557016097714666
44	27.69363784904637	19.846459894928888	24.56238688735996	27.897515368664777
45	28.58919687277896	18.77230196587021	24.82964412107357	27.808857040277257
46	29.704569933835973	19.835835549791543	24.749778354179032	25.709816162193448
47	29.97713934011826	21.030524842649783	21.77807574791726	27.214260069314694
48	30.15335692670667	20.92647953164956	22.159819459404012	26.760344082239758
49	29.004095868228813	19.981462349518974	25.069424600121632	25.945017182130588
50	27.116442822705324	21.06148198623964	23.95386836070018	27.868206830354854
51	26.664358619274758	22.469390895302574	22.92568820111519	27.940562284307475
52	27.740714688706685	20.404787549732927	25.39877929937939	26.455718462180993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	8.0
6	14.0
7	10.0
8	6.0
9	214.5
10	423.0
11	704.0
12	985.0
13	887.0
14	828.0
15	867.0
16	793.5
17	720.0
18	640.5
19	561.0
20	792.0
21	1023.0
22	1388.5
23	1754.0
24	1925.5
25	2097.0
26	2433.0
27	2769.0
28	3450.0
29	4131.0
30	4033.0
31	3935.0
32	4362.5
33	4790.0
34	5359.0
35	5928.0
36	6304.5
37	6681.0
38	6988.5
39	7901.5
40	8507.0
41	10479.0
42	12451.0
43	11894.0
44	11337.0
45	12765.5
46	14194.0
47	15589.5
48	16985.0
49	18466.5
50	19948.0
51	24348.0
52	28748.0
53	33573.0
54	38398.0
55	40062.0
56	41726.0
57	49672.5
58	57619.0
59	51592.5
60	45566.0
61	46235.5
62	46905.0
63	45572.5
64	41087.0
65	37934.0
66	29654.5
67	21375.0
68	19144.0
69	16913.0
70	14783.0
71	12653.0
72	13850.5
73	15048.0
74	11272.0
75	7496.0
76	4714.5
77	1933.0
78	1338.5
79	744.0
80	502.0
81	260.0
82	178.0
83	96.0
84	64.0
85	32.0
86	33.0
87	34.0
88	18.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	545916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.420621487554863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06205591287471	8.344140856835118
2	9.203329751231013	2.1021549102792374
3	3.286445217893403	1.1259974061943594
4	1.531749723322694	0.6997413521494149
5	0.8356456605770927	0.4771796393584361
6	0.5950566987986591	0.40775503923680567
7	0.42985227837746803	0.3436426116838488
8	0.3063499446645388	0.27989654085976595
9	0.2694596371918457	0.27696568702877367
>10	6.179928464882031	19.876867503425437
>50	2.7090317096251626	21.4917679643022
>100	1.496463342261857	33.56908388836378
>500	0.06896883570981764	5.259417199715707
>1k	0.025662822589699583	5.74538940056712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4864	0.8909795646216634	No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4361	0.7988408473098426	No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3245	0.5944137925981287	No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3026	0.5542977307864213	No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2572	0.47113475333201443	No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAG	1584	0.29015452926823904	No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1390	0.25461792656745724	No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1372	0.2513207160075909	No Hit
CGTATTGGGTTGTACATATTGTATTTTTACTATTGAACTGTACTCTAGTGGC	1209	0.22146264260435672	No Hit
CGTATTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	1192	0.2183486104089274	No Hit
CGTATTGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT	1141	0.20900651382263938	No Hit
CGTATTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG	1135	0.20790744363601726	No Hit
CGTATTGGGACAATGCATCGCGGTGGGAGGCAAGCTGGGCTCCCCTGCTCCG	1113	0.20387751961840284	No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1076	0.1970999201342331	No Hit
CGTATTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1071	0.196184028312048	No Hit
CGTATTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1014	0.18574286153913788	No Hit
CGTATTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG	996	0.18244565097927154	No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	970	0.17768301350390903	No Hit
CGTATTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	910	0.16669231163768786	No Hit
CGTATTGGGGAAAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCT	908	0.1663259549088138	No Hit
CGTATTGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG	872	0.15973153378908111	No Hit
CGTATTGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT	871	0.15954835542464407	No Hit
CGTATTGGGGCTTCACACTACGCCGGAAGGGGATGAACCGCTTCCCGGTGCC	837	0.15332029103378542	No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	834	0.15277075594047435	No Hit
CGTATTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	816	0.149473545380608	No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	804	0.14727540500736377	No Hit
CGTATTGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTTC	798	0.14617633482074166	No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	763	0.13976509206544596	No Hit
CGTATTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	757	0.13866602187882385	No Hit
CGTATTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	727	0.13317067094571325	No Hit
CGTATTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	722	0.13225477912352815	No Hit
CGTATTGAGGGAGGTTGAGGCAGGAGAATGGCATGAACCCGGGAAGCGGAGG	704	0.1289575685636618	No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	660	0.12089772052843295	No Hit
CGTATTGGGACTCTTCGTCAGTGCTATCTTCTTGTATGTCATTGTGAGCAAG	658	0.12053136379955891	No Hit
CGTATTGGGGTTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGAT	644	0.11796686669744064	No Hit
CGTATTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	640	0.11723415323969255	No Hit
CGTATTGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT	640	0.11723415323969255	No Hit
CGTATTGGGACTTCCGGCGGTGGCGATTTGGAGGACTGAAGGAAGATGGGTC	637	0.1166846181463815	No Hit
CGTATTGGGGCTCGTTTTAACAATTATCTTTAGGCCTTTGAAGAGCAGCAGG	634	0.11613508305307044	No Hit
CGTATTGGGCAGAAGCCCATCCCTAGTCTCCAAAGCCCTGCCTCTGGGCAAC	627	0.1148528345020113	No Hit
CGTATTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	609	0.11155562394214494	No Hit
CGTATTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	595	0.10899112684002667	No Hit
CGTATTGGGGAAGAACAGTATGTGCTGTTGACCTCTGATCCTTCTCTTCATT	580	0.10624345137347138	No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	576	0.1055107379157233	No Hit
CGTATTGGGAGCGTCTTGTTAAAAAAAAAAAATCAAGAAAGAATTGAGTTTT	575	0.10532755955128628	No Hit
CGTATTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	572	0.10477802445797521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.663567288740392E-4	0.0	0.0	0.0	0.0
8	0.002381318737681255	0.0	0.0	0.0	0.0
9	0.002747675466555294	0.0	0.0	0.0	0.0
10	0.021615047003568314	0.0	0.0	0.0	0.0
11	0.05312172568673569	0.0	0.0	0.0	0.0
12	0.07876669670791843	0.0	0.0	0.0	0.0
13	0.09323778749844298	0.0	0.0	0.0	0.0
14	0.10862477011115264	0.0	0.0	0.0	0.0
15	0.12364539599498824	0.0	0.0	0.0	0.0
16	0.13866602187882385	0.0	0.0	0.0	0.0
17	0.14965672374504502	0.0	0.0	0.0	0.0
18	0.1624792092556364	0.0	0.0	0.0	0.0
19	0.17401944621516863	0.0	0.0	0.0	0.0
20	0.19013914228562637	0.0	0.0	0.0	0.0
21	0.20296162779621774	0.0	0.0	0.0	0.0
22	0.2194476805955495	0.0	0.0	0.0	0.0
23	0.23684962521706637	0.0	0.0	0.0	0.0
24	0.2533356780163981	0.0	0.0	0.0	0.0
25	0.26780676880692267	0.0	0.0	0.0	0.0
26	0.280446075953077	0.0	0.0	0.0	0.0
27	0.2940012749214165	0.0	0.0	0.0	0.0
28	0.3256911319690209	0.0	0.0	0.0	0.0
29	0.34071175785285646	0.0	0.0	0.0	0.0
30	0.3555492053722551	0.0	0.0	0.0	0.0
31	0.36965393943390557	0.0	0.0	0.0	0.0
32	0.3837586734955561	0.0	0.0	0.0	0.0
33	0.39456619699734025	0.0	0.0	0.0	0.0
34	0.40574007722799843	0.0	0.0	0.0	0.0
35	0.4288205511470629	0.0	0.0	0.0	0.0
36	0.4425589284798394	0.0	0.0	0.0	0.0
37	0.45794591109254906	0.0	0.0	0.0	0.0
38	0.46655529422108893	0.0	0.0	0.0	0.0
39	0.4786450662739323	0.0	0.0	0.0	0.0
40	0.4905516599623385	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGCA	35	1.0182521E-7	46.000004	27
CGCGGAA	35	1.0182521E-7	46.000004	34
CCCTAGT	70	0.0	46.000004	21
GGACTAA	55	1.8189894E-12	46.000004	8
ATCCGTA	35	1.0182521E-7	46.000004	42
TAGCATA	35	1.0182521E-7	46.000004	30
TTATAAT	35	1.0182521E-7	46.000004	35
CCGTAAT	35	1.0182521E-7	46.000004	44
TCGTGGC	35	1.0182521E-7	46.000004	21
CACTAGG	35	1.0182521E-7	46.000004	22
ATCCCTA	70	0.0	46.000004	19
GTACTCT	140	0.0	46.000004	40
TACTCTA	140	0.0	46.000004	41
TTAACCT	35	1.0182521E-7	46.000004	39
AATAACG	35	1.0182521E-7	46.000004	11
TGTCATT	90	0.0	46.0	37
ACTATTC	20	6.30853E-4	46.0	22
CTATGGG	25	3.4147815E-5	46.0	9
TCTGATA	20	6.30853E-4	46.0	24
GTACCTC	25	3.4147815E-5	46.0	20
>>END_MODULE