FastQCFastQC Report
Fri 17 Jun 2016
SRR1527465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178251
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC15740.8830244991612951No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9810.5503475436322938No Hit
GAACGCGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTC7470.41907198276587504No Hit
GAACGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC7200.40392480266590364No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6420.36016628237709747No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC6240.3500681623104499No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG5550.31135870205496746No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA5280.29621152195499606No Hit
GAACGCGGGGGGGAAACTTGTAAATCTGAGCCAGTAAAAGAAGAAGGTTC5190.2911624619216722No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA5180.29060145525130293No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG5000.2805033351846553No Hit
GAACGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA4980.27938132184391673No Hit
GAACGCGCGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT4920.2760152818217009No Hit
GAACGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA4860.272649241799485No Hit
GAACGCGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAG4830.2709662217883771No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG4370.24515991495138878No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG4300.2412328682588036No Hit
GAACGCGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGC4300.2412328682588036No Hit
GAACGCGGGAACATGAACTGTGTGAAATTGCATACTGCTGTGACGCTAAC4150.23281776820326394No Hit
GAACGCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA4110.2305737415217867No Hit
GAACGCGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGA3970.22271964813661635No Hit
GAACGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC3890.21823159477366186No Hit
GAACGCGGGGGTAGAGAGGCTCCCCGCCTTTTGGCCTAAGTCCCTGAGCC3850.2159875680921846No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC3840.21542656142181532No Hit
GAACGCGGGGGAGTTTGGGAGCTTTGCGCGTGTGCGCTCATAGTTGCTTT3650.2047674346847984No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA3570.20027938132184392No Hit
GAACGCGGGGACAGTGTGTTGCAAATAGGGCAGAGTTTTTCCTACCTGAG3460.1941083079477815No Hit
GAACGCGGGAACGTGTCTGACCTTGGGCTCTTCCTGAAGGAGCTCCTTAG3420.19186428126630425No Hit
GAACGCGGGCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGATCGG3410.19130327459593494No Hit
GAACGCGGGGAGGCGTGCTGTGCGGCTGGCTGTACGGTGAACTGTCTGGC3380.189620254584827No Hit
GAACGCGGGGAGTGGCCTAGCTCCCTGCGGCTGTCCTTGGTCGCCTTGTC3250.18232716787002598No Hit
GAACGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG3250.18232716787002598No Hit
GAACGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT3220.18064414785891805No Hit
GAACGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT3140.17615609449596356No Hit
GAACGCGGGGAGAGTGAGGTTCTGCCTGCCGGAGGCCGCAGTGGTGCGGA3090.173351061144117No Hit
GAACGCGGGGCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGG3090.173351061144117No Hit
GAACGCGGGATTTGGAGACAAGGGTATCACATTTCAATTTGTAAAACTCT2990.1677409944404239No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT2970.1666189810996853No Hit
GAACGCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA2850.15988690105525355No Hit
GAACGCGGGGTCCAAATCCCAGGATGATGAAATTGGAGATGGGACCACAG2790.15652086103303767No Hit
GAACGCGGGAGGCGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCG2760.15483784102192977No Hit
GAACGCGGGAACCAACCTTTCCTCTTTGTTCTGAGGGACACGGACACGGG2750.15427683435156042No Hit
GAACGCGGGATTGGAGATAAAGAAAAGCTGGGAGGTAAACTTTCTTCTGA2750.15427683435156042No Hit
GAACGCGGGGGTCTCAGTTTGCACAGTGGGTAGGGCTGGGGAGGGGGTTT2740.15371582768119113No Hit
GAACGCGGGGAAACAGGCGAAGGCTGGGCGGGGTGGGGACCCCCAGCTCT2720.15259381434045252No Hit
GAACGCGGGGAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTAT2710.1520328076700832No Hit
GAACGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGA2700.15147180099971388No Hit
GAACGCGGGATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA2680.15034978765897528No Hit
GAACGCGGGGAATGGTGTCTTTAGACATGACCAGCAGCTCTTCGCCGACT2670.14978878098860596No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT2650.14866676764786732No Hit
GAACGCGGGATATCTAGATATATAATTATATGTGTGAATAAATGTGACAG2590.14530072762565147No Hit
GAACGCGGGGAGTTTTTTTAGATGCTTTGGATTCTTTAACGTGTGCTAAA2500.14025166759232766No Hit
GAACGCGGGGAGATCTCACAGAGCTGTTTCAAAGATGAACGCCAGAGGTC2480.13912965425158905No Hit
GAACGCGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG2480.13912965425158905No Hit
GAACGCGGGGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGTCG2480.13912965425158905No Hit
GAACGCGGGAGGTTGGGGATAAAGTGTCCTTCTCTAGATAGTTGGCAAGC2470.13856864758121976No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTGAGAGCACCCGACTGCT2460.13800764091085044No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG2430.1363246208997425No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA2430.1363246208997425No Hit
GAACGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG2400.13464160088863455No Hit
GAACGCGGGACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCC2400.13464160088863455No Hit
GAACGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC2350.13183656753678802No Hit
GAACGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC2320.1301535475256801No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2310.12959254085531077No Hit
GAACGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC2310.12959254085531077No Hit
GAACGCGGGAGGCCCCCTGCTGGGAAACCCATACCCAGCATCCCTCCCCT2280.12790952084420285No Hit
GAACGCGGGCGAAGGCACCACAAGAATGGAACTATTTTAAGCTTCCATTT2280.12790952084420285No Hit
GAACGCGGGGGACTGTGTTTTAAAACCTAGTGAACCATGACTCGGAGCCA2270.12734851417383353No Hit
GAACGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT2250.1262265008330949No Hit
GAACGCGGGAAGGTCGTTATCACCAGGCTAAAGCTGGACAAGGACCGCAA2240.1256654941627256No Hit
GAACGCGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGT2240.1256654941627256No Hit
GAACGCGGGGAATTAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTG2230.12510448749235628No Hit
GAACGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA2230.12510448749235628No Hit
GAACGCGGGGGCGGGGATCTGCGCGGCGGCGGAGGCGGGGACCTCTGGCC2230.12510448749235628No Hit
GAACGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC2220.12454348082198696No Hit
GAACGCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC2210.12398247415161766No Hit
GAACGCGGGGCCATCCATCCTCAGATGAGCCACCTGTACCCTTCTGACAG2200.12342146748124834No Hit
GAACGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG2170.12173844747014043No Hit
GAACGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT2170.12173844747014043No Hit
GAACGCGGGAGGGAATGATGTTTCATGGTTTCTACCTCAAAAAGTGAAGA2160.12117744079977111No Hit
GAACGCGGGAGGTTTTAGGATGGTCACAATGAAAACCTGTGGGTTTGAGG2160.12117744079977111No Hit
GAACGCGGGAACATGTGGAACGTGTGCTGGGGGCCGTCGCTAACTCTTAA2140.12005542745903248No Hit
GAACGCGGGGAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAA2120.11893341411829386No Hit
GAACGCGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC2100.11781140077755524No Hit
GAACGCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG2090.11725039410718593No Hit
GAACGCGTAGCGTAGAGCTTTATGCAGAGAAAGTGGCCACAAGAGGTCTG2070.11612838076644733No Hit
GAACGCGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTG2060.11556737409607801No Hit
GAACGCGGGGGAGTGCCTGGCTTGTCCAACCTCGCCCAGGCAGGCTTAGC2050.1150063674257087No Hit
GAACGCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGAT2050.1150063674257087No Hit
GAACGCGGGGTTTTGAGGCTCTTCCTGTACTCTTAGGATATATGAGCCGC2040.11444536075533938No Hit
GAACGCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT2000.11220133407386214No Hit
GAACGCGGGAGTACGTTTCACAAGGAGTGGTGGCAATGCCACGTTACAGG1990.11164032740349282No Hit
GAACGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT1960.10995730739238489No Hit
GAACGCGGGGGGTTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT1930.10827428738127695No Hit
GAACGCGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGG1930.10827428738127695No Hit
GAACGCGGGAGCGTTGCCGGGCCGGTTCTCCGCCGCCTCCCGGATCTCTG1920.10771328071090766No Hit
GAACGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC1900.10659126737016904No Hit
GAACGCGGGGTACCTCTCTAATGCTGGTGGCAATTCTACCCAACCCTGTG1900.10659126737016904No Hit
GAACGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC1900.10659126737016904No Hit
GAACGCGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAG1880.10546925402943041No Hit
GAACGCGGGAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT1870.1049082473590611No Hit
GAACGCGGGGAAAGTGCCATTGACCACCGCTGCATCCGGGCTGTACAAGA1870.1049082473590611No Hit
GAACGCGGGGAAGACACTGACACTAGTAGCAAAGGAGGCTGTGCCCAGCA1850.10378623401832247No Hit
GAACGCGGGGCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAA1850.10378623401832247No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG1840.10322522734795318No Hit
GAACGCGGGGACTAGGGGGTTTCCGGTTCCGCTGTCCTCTTTCTCTTTCC1840.10322522734795318No Hit
GAACGCGGGGGCTGGTGAGATGGCTTGGCGGTTAAGAGCACTGACTGCTC1840.10322522734795318No Hit
GAACGCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA1800.10098120066647591No Hit
GAACGCGGGGATCCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAG1800.10098120066647591No Hit
GAACGCGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCA1790.10042019399610662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT4050.044.0000046
CGGACAT207.8385725E-444.038
TCGCCGA408.245479E-944.042
GTTGAGA351.4368925E-744.040
CATGGTT408.245479E-944.024
CTGCTAT254.4276727E-544.024
ATCCTCG254.4276727E-544.042
CTATGTC207.8385725E-444.025
TTTAGAA207.8385725E-444.032
CAGGTAA207.8385725E-444.019
TGATATC351.4368925E-744.039
CGGGTAT351.4368925E-744.06
TAGAGCT302.5156514E-644.013
AATCCCA254.4276727E-544.015
AAATCTG551.8189894E-1244.022
TCGTTAT302.5156514E-644.014
TCGTTAG207.8385725E-444.041
TCCATCA207.8385725E-444.014
CAAACGG254.4276727E-544.031
AAATCCC254.4276727E-544.014
Produced by FastQC (version 0.11.5)