FastQCFastQC Report
Fri 17 Jun 2016
SRR1527464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156897
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA15801.007030089804139No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG7400.4716470040854829No Hit
GAACGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC6590.4200207779626124No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC5910.37668024245205456No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG5900.3760428816357228No Hit
GAACGCGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA5880.37476816000305935No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT5740.36584510857441505No Hit
GAACGCGGGGGGGAAACTTGTAAATCTGAGCCAGTAAAAGAAGAAGGTTCGG5430.3460869232681313No Hit
GAACGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG5140.3276034595945111No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG5140.3276034595945111No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA4890.3116694391862177No Hit
GAACGCGCGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT4690.29892222285958303No Hit
GAACGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG4490.28617500653294836No Hit
GAACGCGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGTC4240.270240986124655No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA4110.2619552955123425No Hit
GAACGCGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCT3900.24857071836937608No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3890.24793335755304435No Hit
GAACGCGGGGGTAGAGAGGCTCCCCGCCTTTTGGCCTAAGTCCCTGAGCCCA3650.23263669796108277No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC3580.2281751722467606No Hit
GAACGCGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGAGG3480.22180156408344326No Hit
GAACGCGGGAACATGAACTGTGTGAAATTGCATACTGCTGTGACGCTAACCT3460.2205268424507798No Hit
GAACGCGGGGGAGTTTGGGAGCTTTGCGCGTGTGCGCTCATAGTTGCTTTCG3440.21925212081811635No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC3410.21734003836912114No Hit
GAACGCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC3410.21734003836912114No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG3330.21224115183846726No Hit
GAACGCGGGGGTCTCAGTTTGCACAGTGGGTAGGGCTGGGGAGGGGGTTTGT3200.20395546122615474No Hit
GAACGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT3100.1975818530628374No Hit
GAACGCGGGGGCGGGGATCTGCGCGGCGGCGGAGGCGGGGACCTCTGGCCTC3080.19630713143017395No Hit
GAACGCGGGGAGTGGCCTAGCTCCCTGCGGCTGTCCTTGGTCGCCTTGTCAC2990.19057088408318834No Hit
GAACGCGGGGACAGTGTGTTGCAAATAGGGCAGAGTTTTTCCTACCTGAGTA2990.19057088408318834No Hit
GAACGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA2980.1899335232668566No Hit
GAACGCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC2920.1861093583688662No Hit
GAACGCGGGCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGATCGGCG2890.184197275919871No Hit
GAACGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAG2860.1822851934708758No Hit
GAACGCGGGAACGTGTCTGACCTTGGGCTCTTCCTGAAGGAGCTCCTTAGGC2850.18164783265454407No Hit
GAACGCGGGGTCCAAATCCCAGGATGATGAAATTGGAGATGGGACCACAGGA2840.1810104718382123No Hit
GAACGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC2820.17973575020554886No Hit
GAACGCGGGGAGGCGTGCTGTGCGGCTGGCTGTACGGTGAACTGTCTGGCCT2780.17718630694022192No Hit
GAACGCGGGATTGGAGATAAAGAAAAGCTGGGAGGTAAACTTTCTTCTGAAG2770.1765489461238902No Hit
GAACGCGGGCGAAGGCACCACAAGAATGGAACTATTTTAAGCTTCCATTTTT2750.17527422449122673No Hit
GAACGCGGGGAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC2740.17463686367489498No Hit
GAACGCGGGAGGCGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCGCC2730.17399950285856325No Hit
GAACGCGGGATTTGGAGACAAGGGTATCACATTTCAATTTGTAAAACTCTTT2730.17399950285856325No Hit
GAACGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG2500.1593402040829334No Hit
GAACGCGGGGCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAG2500.1593402040829334No Hit
GAACGCGGGGAGAGTGAGGTTCTGCCTGCCGGAGGCCGCAGTGGTGCGGAGA2450.15615340000127473No Hit
GAACGCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC2440.155516039184943No Hit
GAACGCGGGGAAACAGGCGAAGGCTGGGCGGGGTGGGGACCCCCAGCTCTGA2420.15424131755227952No Hit
GAACGCGGGGAATGGTGTCTTTAGACATGACCAGCAGCTCTTCGCCGACTTT2400.15296659591961606No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG2400.15296659591961606No Hit
GAACGCGGGATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACG2370.15105451347062085No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC2360.15041715265428912No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT2350.1497797918379574No Hit
GAACGCGGGAACCAACCTTTCCTCTTTGTTCTGAGGGACACGGACACGGGGG2340.14914243102162564No Hit
GAACGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA2330.1485050702052939No Hit
GAACGCGGGACTCGGGAGGCAGGGGCAGGCGAATTTCTGAGTTCAAGGCCAG2260.1440435444909718No Hit
GAACGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT2240.1427688228583083No Hit
GAACGCGGGGAGATCTCACAGAGCTGTTTCAAAGATGAACGCCAGAGGTCTT2230.14213146204197658No Hit
GAACGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG2210.14085674040931312No Hit
GAACGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT2210.14085674040931312No Hit
GAACGCGGGATATCTAGATATATAATTATATGTGTGAATAAATGTGACAGAA2150.1370325755113227No Hit
GAACGCGGGGAGTTTTTTTAGATGCTTTGGATTCTTTAACGTGTGCTAAAAG2130.13575785387865924No Hit
GAACGCGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG2100.13384577142966403No Hit
GAACGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG2070.13193368898066885No Hit
GAACGCGGGGGACTGTGTTTTAAAACCTAGTGAACCATGACTCGGAGCCAGC2070.13193368898066885No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTGAGAGCACCCGACTGCTCT2070.13193368898066885No Hit
GAACGCGGGAGGTTGGGGATAAAGTGTCCTTCTCTAGATAGTTGGCAAGCTC2070.13193368898066885No Hit
GAACGCGGGGTTTTGAGGCTCTTCCTGTACTCTTAGGATATATGAGCCGCCC2050.1306589673480054No Hit
GAACGCGGGAGGCCCCCTGCTGGGAAACCCATACCCAGCATCCCTCCCCTGC2040.13002160653167366No Hit
GAACGCGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT2030.1293842457153419No Hit
GAACGCGGGGATCCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAA2030.1293842457153419No Hit
GAACGCGGGGCCATCCATCCTCAGATGAGCCACCTGTACCCTTCTGACAGTG2030.1293842457153419No Hit
GAACGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG2020.12874688489901018No Hit
GAACGCGGGAACATGTGGAACGTGTGCTGGGGGCCGTCGCTAACTCTTAACT2020.12874688489901018No Hit
GAACGCGGGGGGTTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTTT2020.12874688489901018No Hit
GAACGCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC2010.12810952408267845No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC2010.12810952408267845No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG2000.12747216326634672No Hit
GAACGCGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC1990.126834802450015No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT1990.126834802450015No Hit
GAACGCGGGGAAAGTGCCATTGACCACCGCTGCATCCGGGCTGTACAAGACG1980.12619744163368327No Hit
GAACGCGGGAAGGTCGTTATCACCAGGCTAAAGCTGGACAAGGACCGCAAGA1970.1255600808173515No Hit
GAACGCGGGGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTA1970.1255600808173515No Hit
GAACGCGGGGGAGCGATCGCAGCGGAGAACGAGATGCCGGTGGCCGTGGGTC1960.12492272000101977No Hit
GAACGCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC1950.12428535918468804No Hit
GAACGCGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCTG1940.12364799836835631No Hit
GAACGCGGGAGGGAATGATGTTTCATGGTTTCTACCTCAAAAAGTGAAGACA1940.12364799836835631No Hit
GAACGCGGGGACTAGGGGGTTTCCGGTTCCGCTGTCCTCTTTCTCTTTCCCT1920.12237327673569284No Hit
GAACGCGGGAGCGTTGCCGGGCCGGTTCTCCGCCGCCTCCCGGATCTCTGAG1900.12109855510302939No Hit
GAACGCGGGACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCAT1900.12109855510302939No Hit
GAACGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA1900.12109855510302939No Hit
GAACGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT1890.12046119428669765No Hit
GAACGCGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAG1890.12046119428669765No Hit
GAACGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT1860.11854911183770245No Hit
GAACGCGGGGGCTGGTGAGATGGCTTGGCGGTTAAGAGCACTGACTGCTCTT1850.11791175102137072No Hit
GAACGCGGGACTCGCCGCTGCAGCCCCTGTCGTGCGTGCGCTCTCATCCAGC1850.11791175102137072No Hit
GAACGCGGGGTAGTTCCATGATGGCGTCGGCGGTGCTGTCAGCGTCTCGGGT1850.11791175102137072No Hit
GAACGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG1840.11727439020503896No Hit
GAACGCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT1840.11727439020503896No Hit
GAACGCGGGGGAGTGCCTGGCTTGTCCAACCTCGCCCAGGCAGGCTTAGCTT1830.11663702938870724No Hit
GAACGCGGGAGTACGTTTCACAAGGAGTGGTGGCAATGCCACGTTACAGGTG1820.11599966857237551No Hit
GAACGCGGGGAGCCTTTTATTGACCAGTTAACTTGGGGAGCAAGGTTTGCAC1800.11472494693971205No Hit
GAACGCGGGAGTGCAGTGCTGAACGCGGGGCACCTGAATCTTAGCCACCGTA1790.1140875861233803No Hit
GAACGCGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAGC1790.1140875861233803No Hit
GAACGCGGGGCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAG1790.1140875861233803No Hit
GAACGCGGGAGGTTTTAGGATGGTCACAATGAAAACCTGTGGGTTTGAGGTG1780.11345022530704857No Hit
GAACGCGGGGGAGGACCCCAACATGAAGCCAACCCCCAAGGCCACAACTGAC1770.11281286449071684No Hit
GAACGCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT1750.11153814285805339No Hit
GAACGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA1720.10962606040905817No Hit
GAACGCGGGGGTTCTTCCGGTCGCGAGCACCCCAGGGTGTAGAGGGCGGTCG1720.10962606040905817No Hit
GAACGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG1690.10771397796006296No Hit
GAACGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG1690.10771397796006296No Hit
GAACGCGGGAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGC1670.1064392563273995No Hit
GAACGCGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAAG1650.10516453469473604No Hit
GAACGCGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG1650.10516453469473604No Hit
GAACGCGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCC1640.1045271738784043No Hit
GAACGCGGGACATGTTGGTGAAGGAAAAAGCTGGGTGGGAGATGATGAAATA1630.10388981306207257No Hit
GAACGCGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT1620.10325245224574084No Hit
GAACGCGGGGTACCTCTCTAATGCTGGTGGCAATTCTACCCAACCCTGTGTA1610.1026150914294091No Hit
GAACGCGGGGAATTAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGT1610.1026150914294091No Hit
GAACGCGGGAGATCCTTGATGCCTGTGCCTATGTGTTTGGTAACAACAAACT1600.10197773061307737No Hit
GAACGCGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTC1580.10070300898041391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT206.294717E-446.032
TGATCCT206.294717E-446.018
GTTGATC206.294717E-446.016
GTGTTTA206.294717E-446.09
AAACTTG551.8189894E-1246.014
ATCCTCG253.4035846E-546.042
ACTATTT405.5588316E-946.031
AACGTCC206.294717E-446.021
GACAGTC253.4035846E-546.09
TGATATC253.4035846E-546.039
CGGAAGG206.294717E-446.028
TGATAGG206.294717E-446.028
TCGTTGA253.4035846E-546.045
TCGTTAT206.294717E-446.014
AAATCAA206.294717E-446.039
ACACGAG206.294717E-446.038
ATTAGTC253.4035846E-546.012
CTGTATT206.294717E-446.042
AATTTGT253.4035846E-546.036
GATGTCC206.294717E-446.024
Produced by FastQC (version 0.11.5)