FastQCFastQC Report
Fri 17 Jun 2016
SRR1527463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176017
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA16160.9180931387309181No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG10410.5914201469176272No Hit
GAACGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC7410.42098206423243206No Hit
GAACGCGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA7340.4170051756364442No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT6480.3681462586000216No Hit
GAACGCGGGGGGGAAACTTGTAAATCTGAGCCAGTAAAAGAAGAAGGTTCGG5980.3397399114858224No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC5900.33519489594755053No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5620.31928734156359895No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG5380.3056522949487834No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG5370.3050841680064994No Hit
GAACGCGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC5350.3039479141219314No Hit
GAACGCGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGTC5190.2948578830453877No Hit
GAACGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG5130.2914491213916838No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG5080.28860848668026384No Hit
GAACGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG4980.282927217257424No Hit
GAACGCGCGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT4850.2755415670077322No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC4680.26588340898890445No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA4510.25622525097007676No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC4280.24315833129754513No Hit
GAACGCGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCT4190.23804518881698927No Hit
GAACGCGGGAACATGAACTGTGTGAAATTGCATACTGCTGTGACGCTAACCT4070.23122766550958146No Hit
GAACGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT4040.22952328468272953No Hit
GAACGCGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGAGG3860.2192969997216178No Hit
GAACGCGGGGGTAGAGAGGCTCCCCGCCTTTTGGCCTAAGTCCCTGAGCCCA3830.21759261889476586No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG3730.211911349471926No Hit
GAACGCGGGCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGATCGGCG3700.21020696864507407No Hit
GAACGCGGGGGAGTTTGGGAGCTTTGCGCGTGTGCGCTCATAGTTGCTTTCG3600.2045256992222342No Hit
GAACGCGGGAACGTGTCTGACCTTGGGCTCTTCCTGAAGGAGCTCCTTAGGC3580.20338944533766626No Hit
GAACGCGGGGAGTGGCCTAGCTCCCTGCGGCTGTCCTTGGTCGCCTTGTCAC3440.19543566814569047No Hit
GAACGCGGGGGTCTCAGTTTGCACAGTGGGTAGGGCTGGGGAGGGGGTTTGT3340.18975439872285063No Hit
GAACGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA3320.18861814483828268No Hit
GAACGCGGGGTCCAAATCCCAGGATGATGAAATTGGAGATGGGACCACAGGA3320.18861814483828268No Hit
GAACGCGGGGAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC3310.18805001789599868No Hit
GAACGCGGGGACAGTGTGTTGCAAATAGGGCAGAGTTTTTCCTACCTGAGTA3230.18350500235772682No Hit
GAACGCGGGGAGGCGTGCTGTGCGGCTGGCTGTACGGTGAACTGTCTGGCCT3200.18180062153087484No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG3160.1795281137617389No Hit
GAACGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC3120.17725560599260298No Hit
GAACGCGGGGGCGGGGATCTGCGCGGCGGCGGAGGCGGGGACCTCTGGCCTC3100.17611935210803503No Hit
GAACGCGGGGCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAG3050.1732787173966151No Hit
GAACGCGGGATTTGGAGACAAGGGTATCACATTTCAATTTGTAAAACTCTTT3050.1732787173966151No Hit
GAACGCGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC3020.17157433656976315No Hit
GAACGCGGGGAGAGTGAGGTTCTGCCTGCCGGAGGCCGCAGTGGTGCGGAGA2980.16930182880062722No Hit
GAACGCGGGGAATGGTGTCTTTAGACATGACCAGCAGCTCTTCGCCGACTTT2950.16759744797377527No Hit
GAACGCGGGAGGCGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCGCC2870.16305243243550338No Hit
GAACGCGGGATTGGAGATAAAGAAAAGCTGGGAGGTAAACTTTCTTCTGAAG2840.16134805160865143No Hit
GAACGCGGGGAAACAGGCGAAGGCTGGGCGGGGTGGGGACCCCCAGCTCTGA2830.16077992466636745No Hit
GAACGCGGGGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTA2760.15680303607037957No Hit
GAACGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG2760.15680303607037957No Hit
GAACGCGGGATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACG2720.1545305283012436No Hit
GAACGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAG2680.15225802053210769No Hit
GAACGCGGGAACCAACCTTTCCTCTTTGTTCTGAGGGACACGGACACGGGGG2650.15055363970525576No Hit
GAACGCGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG2560.1454404972246999No Hit
GAACGCGGGATATCTAGATATATAATTATATGTGTGAATAAATGTGACAGAA2540.14430424334013192No Hit
GAACGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG2530.14373611639784792No Hit
GAACGCGGGACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCAT2510.14259986251327997No Hit
GAACGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT2510.14259986251327997No Hit
GAACGCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC2480.14089548168642801No Hit
GAACGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG2470.14032735474414404No Hit
GAACGCGGGAGGGAATGATGTTTCATGGTTTCTACCTCAAAAAGTGAAGACA2470.14032735474414404No Hit
GAACGCGGGAGGTTGGGGATAAAGTGTCCTTCTCTAGATAGTTGGCAAGCTC2450.13919110085957606No Hit
GAACGCGGGGAGTTTTTTTAGATGCTTTGGATTCTTTAACGTGTGCTAAAAG2440.13862297391729209No Hit
GAACGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA2440.13862297391729209No Hit
GAACGCGGGGAGATCTCACAGAGCTGTTTCAAAGATGAACGCCAGAGGTCTT2410.13691859309044013No Hit
GAACGCGGGGGACTGTGTTTTAAAACCTAGTGAACCATGACTCGGAGCCAGC2400.13635046614815613No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT2370.1346460853213042No Hit
GAACGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT2360.1340779583790202No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTGAGAGCACCCGACTGCTCT2340.13294170449445222No Hit
GAACGCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT2340.13294170449445222No Hit
GAACGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG2320.13180545060988427No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2290.13010106978303232No Hit
GAACGCGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCC2270.12896481589846437No Hit
GAACGCGGGCGAAGGCACCACAAGAATGGAACTATTTTAAGCTTCCATTTTT2230.1266923081293284No Hit
GAACGCGGGAAGGTCGTTATCACCAGGCTAAAGCTGGACAAGGACCGCAAGA2220.12612418118704444No Hit
GAACGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT2190.12441980036019247No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC2150.12214729259105654No Hit
GAACGCGGGGTACCTCTCTAATGCTGGTGGCAATTCTACCCAACCCTGTGTA2130.12101103870648858No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC2120.1204429117642046No Hit
GAACGCGGGAACATGTGGAACGTGTGCTGGGGGCCGTCGCTAACTCTTAACT2120.1204429117642046No Hit
GAACGCGGGGCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAG2120.1204429117642046No Hit
GAACGCGGGGTTTTGAGGCTCTTCCTGTACTCTTAGGATATATGAGCCGCCC2120.1204429117642046No Hit
GAACGCGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCTG2100.11930665787963662No Hit
GAACGCGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC2100.11930665787963662No Hit
GAACGCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC2100.11930665787963662No Hit
GAACGCGGGGAATTAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGT2090.11873853093735263No Hit
GAACGCGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG2090.11873853093735263No Hit
GAACGCGGGAGGCCCCCTGCTGGGAAACCCATACCCAGCATCCCTCCCCTGC2080.11817040399506866No Hit
GAACGCGGGGGAGTGCCTGGCTTGTCCAACCTCGCCCAGGCAGGCTTAGCTT2060.1170341501105007No Hit
GAACGCGGGGGGTTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTTT2060.1170341501105007No Hit
GAACGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG2040.11589789622593273No Hit
GAACGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG2020.11476164234136477No Hit
GAACGCGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAG2020.11476164234136477No Hit
GAACGCGGGGAAAGTGCCATTGACCACCGCTGCATCCGGGCTGTACAAGACG2010.11419351539908076No Hit
GAACGCGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT2000.1136253884567968No Hit
GAACGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT1950.11078475374537687No Hit
GAACGCGGGGGACGCCGCCGAGGTCGCACGCGTGAGCATGCGCTACGTCGCC1940.11021662680309287No Hit
GAACGCGGGAGCGTTGCCGGGCCGGTTCTCCGCCGCCTCCCGGATCTCTGAG1940.11021662680309287No Hit
GAACGCGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTC1930.1096484998608089No Hit
GAACGCGGGGGCTGGTGAGATGGCTTGGCGGTTAAGAGCACTGACTGCTCTT1900.10794411903395694No Hit
GAACGCGGGGATCCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAA1900.10794411903395694No Hit
GAACGCGGGGTAGTTCCATGATGGCGTCGGCGGTGCTGTCAGCGTCTCGGGT1900.10794411903395694No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG1890.10737599209167296No Hit
GAACGCGGGAGTGCAGTGCTGAACGCGGGGCACCTGAATCTTAGCCACCGTA1860.10567161126482101No Hit
GAACGCGGGAGGTTTTAGGATGGTCACAATGAAAACCTGTGGGTTTGAGGTG1860.10567161126482101No Hit
GAACGCGGGGCCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGA1860.10567161126482101No Hit
GAACGCGGGGAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGT1840.10453535738025305No Hit
GAACGCGGGAGATCCTTGATGCCTGTGCCTATGTGTTTGGTAACAACAAACT1840.10453535738025305No Hit
GAACGCGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT1830.10396723043796907No Hit
GAACGCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC1820.10339910349568507No Hit
GAACGCGGGATCCCAGGCATTGGGAACTTTGGTGATCGCTACTTTGGGACAG1820.10339910349568507No Hit
GAACGCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT1820.10339910349568507No Hit
GAACGCGGGACTCGGGAGGCAGGGGCAGGCGAATTTCTGAGTTCAAGGCCAG1810.1028309765534011No Hit
GAACGCGGGGGAGCGATCGCAGCGGAGAACGAGATGCCGGTGGCCGTGGGTC1810.1028309765534011No Hit
GAACGCGGGAGCTGCCCTTCATTCCCACCTGCTGCCAAAGTCCCTGTGCTAA1800.1022628496111171No Hit
GAACGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA1800.1022628496111171No Hit
GAACGCGGGTGCACCACCAAGCAGCAGAGCACCTTTTACTGATGGAAAAAAA1790.10169472266883313No Hit
GAACGCGGGACTCGCCGCTGCAGCCCCTGTCGTGCGTGCGCTCTCATCCAGC1790.10169472266883313No Hit
GAACGCGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCCCT1770.10055846878426516No Hit
GAACGCGGGAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGC1770.10055846878426516No Hit
GAACGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG1770.10055846878426516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTT551.8189894E-1246.00000427
ATTAGAG551.8189894E-1246.00000421
GATCGGC551.8189894E-1246.00000445
CGACCCG551.8189894E-1246.00000432
TCGATAC551.8189894E-1246.00000425
ATACTTC551.8189894E-1246.00000428
GAAACTT551.8189894E-1246.00000413
TCAGACG551.8189894E-1246.00000422
CGGGATA1550.046.0000046
GACATGT551.8189894E-1246.0000049
CGATACT551.8189894E-1246.00000426
GTAAATC551.8189894E-1246.00000420
TCTATGG551.8189894E-1246.00000420
ACTGGTC551.8189894E-1246.00000432
TAATCAT206.296824E-446.033
TCACGGT253.405293E-546.032
CGGACAT206.296824E-446.038
TCAAAAC253.405293E-546.038
AGCACAC301.8523679E-646.026
CCTTCGG301.8523679E-646.019
Produced by FastQC (version 0.11.5)