##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230241
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.629648933074474	31.0	31.0	33.0	30.0	34.0
2	31.965258142554976	33.0	31.0	34.0	30.0	34.0
3	32.65244678402196	34.0	31.0	34.0	31.0	34.0
4	35.98601465421015	37.0	35.0	37.0	35.0	37.0
5	35.93413423325993	37.0	35.0	37.0	35.0	37.0
6	35.99979152279568	37.0	35.0	37.0	35.0	37.0
7	36.55101393756976	37.0	37.0	37.0	35.0	37.0
8	36.68659361277965	37.0	37.0	37.0	35.0	37.0
9	38.61135505839533	39.0	39.0	39.0	39.0	39.0
10	36.426218614408384	38.0	35.0	39.0	31.0	39.0
11	36.42852055020609	37.0	35.0	39.0	33.0	39.0
12	36.66393474663505	39.0	37.0	39.0	33.0	39.0
13	36.87391472413688	39.0	37.0	39.0	33.0	39.0
14	37.711945309480065	40.0	37.0	40.0	33.0	40.0
15	37.765124369682205	40.0	37.0	40.0	34.0	40.0
16	37.9461086426831	40.0	37.0	40.0	34.0	40.0
17	37.88907275420103	40.0	37.0	40.0	34.0	40.0
18	37.72543117863456	39.0	37.0	40.0	34.0	40.0
19	37.690810932892056	39.0	37.0	40.0	34.0	40.0
20	37.60115704848398	39.0	36.0	40.0	34.0	40.0
21	37.47993189744659	39.0	36.0	40.0	33.0	40.0
22	37.26567813725618	39.0	36.0	40.0	33.0	40.0
23	37.08744750066235	38.0	36.0	40.0	33.0	40.0
24	36.862283433445825	38.0	35.0	40.0	33.0	40.0
25	36.67607854378673	38.0	35.0	40.0	33.0	40.0
26	36.05489465386269	38.0	35.0	40.0	31.0	40.0
27	35.959981063320605	37.0	35.0	39.0	31.0	40.0
28	35.74474137968477	37.0	34.0	39.0	31.0	40.0
29	35.293922455166545	36.0	34.0	38.0	30.0	40.0
30	34.967642600579396	36.0	34.0	38.0	30.0	40.0
31	34.81802111700349	35.0	34.0	38.0	30.0	40.0
32	34.672499685112555	35.0	33.0	38.0	30.0	40.0
33	34.25858556903419	35.0	33.0	38.0	29.0	39.0
34	33.9180988616276	35.0	33.0	38.0	28.0	39.0
35	33.85483471666645	35.0	33.0	38.0	28.0	39.0
36	33.44100746608988	35.0	32.0	37.0	27.0	39.0
37	33.22743125681351	35.0	32.0	37.0	27.0	38.0
38	32.861775270260296	35.0	31.0	37.0	25.0	38.0
39	32.02764494594794	34.0	31.0	36.0	23.0	38.0
40	31.904361082517884	34.0	31.0	36.0	23.0	38.0
41	31.446002232443398	34.0	30.0	35.0	22.0	38.0
42	31.23882366737462	33.0	29.0	35.0	22.0	38.0
43	31.001337728727723	33.0	29.0	35.0	22.0	38.0
44	30.629053904387142	33.0	28.0	35.0	21.0	37.0
45	30.2206079716471	33.0	27.0	35.0	20.0	37.0
46	30.301088859065068	33.0	28.0	35.0	20.0	37.0
47	29.702963416593917	32.0	27.0	35.0	20.0	37.0
48	29.13904126545663	31.0	26.0	35.0	19.0	36.0
49	28.313749505952458	31.0	24.0	34.0	18.0	35.0
50	30.681507637649247	33.0	28.0	35.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	6.0
15	19.0
16	19.0
17	49.0
18	63.0
19	134.0
20	192.0
21	277.0
22	467.0
23	714.0
24	1030.0
25	1379.0
26	2022.0
27	2759.0
28	3543.0
29	4784.0
30	6262.0
31	8771.0
32	13876.0
33	18800.0
34	26914.0
35	52162.0
36	69937.0
37	15618.0
38	441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.27528546175529	0.5303138884907553	1.0558501743824948	0.1385504753714586
8	98.99757210922469	0.6766822590242398	0.1489743355874931	0.1767712961635851
9	96.27086400771366	2.659821665124804	0.45430657441550376	0.6150077527460357
10	37.64577117020861	49.100724892612526	5.260574789025412	7.992929148153456
11	28.023245208281754	28.040183981132817	31.02488262299069	12.911688187594738
12	25.736945200898187	24.2902002684144	28.273852180975585	21.699002349711822
13	23.54967186556695	25.89417175915671	31.865306352908473	18.690850022367865
14	25.544972441919555	30.207478251049984	25.866809126089617	18.38074018094084
15	21.647751703649654	34.81091551895623	26.484857171398662	17.05647560599546
16	23.118384649128522	38.02971668816588	22.4469143202123	16.4049843424933
17	22.57113198778671	37.36128665181266	21.37282239045174	18.69475896994888
18	19.490881293948515	39.01564013359914	22.97592522617605	18.517553346276294
19	18.828531842721322	40.34424798363454	24.65850999604762	16.16871017759652
20	21.893581073744468	37.58974292154742	24.927358724119507	15.589317280588599
21	21.527008656147252	40.11622604140879	24.02091721283351	14.335848089610451
22	18.09973028261691	39.68146420489835	25.074161422161996	17.14464409032275
23	17.090787479206572	42.36821417558124	25.23833722056454	15.302661124647651
24	16.86319986448982	45.20133251679761	22.0859881602321	15.849479458480461
25	19.58295872585682	45.395042585812256	20.492006202196826	14.529992486134095
26	17.97811858009651	46.05131145191343	20.109797994275564	15.860771973714499
27	17.760520498086787	44.25493287468348	21.204303316959187	16.780243310270542
28	17.817851729274977	44.766136352778176	23.001984876716136	14.414027041230709
29	19.700661480796207	41.46437862934925	21.784564868985107	17.050395020869434
30	19.26850560933978	41.40487576061605	22.297071329606805	17.02954730043737
31	18.304298539356587	43.16129620701786	22.334857822889926	16.19954743073562
32	19.487841001385505	43.27639299690325	22.955511833252984	14.280254168458267
33	18.62222627594564	44.46167276896817	20.50416737244887	16.41193358263732
34	17.4321689012817	45.043237303521096	20.808630956258877	16.71596283893833
35	17.424785333628677	46.08475467010654	21.242089810242312	15.248370186022472
36	17.90906050616528	45.864984950551815	20.590598546740154	15.635355996542751
37	17.43824948640772	45.6799614317172	24.003544112473453	12.878244969401628
38	18.811593069870266	44.35613118428082	20.649232760455348	16.183042985393566
39	17.143341107795745	44.3496162716458	21.64948901368566	16.8575536068728
40	19.606412411342898	43.931793207986416	21.250342032913338	15.211452347757351
41	16.098349121138288	45.591792947389905	21.62820696574459	16.681650965727215
42	18.20440321228626	46.16640824179881	19.440933630413348	16.18825491550158
43	17.50383294026694	48.086570159094165	20.66834317085141	13.741253729787484
44	18.152283911206084	47.30304333285557	19.28805034724484	15.256622408693499
45	17.94858430948441	48.058773198518075	18.703011192619908	15.289631299377609
46	17.38917047789056	48.146941682845366	20.174078465607774	14.289809373656299
47	17.868233720319143	50.145282551760985	17.54118510604106	14.445298621878813
48	16.947025073727097	50.592639886032465	17.614152127553304	14.846182912687143
49	17.974643960024498	47.15189735972307	18.00895583323561	16.864502847016823
50	15.164110649276195	48.92221628641293	20.53761059064198	15.376062473668895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	4.0
6	6.0
7	64.5
8	123.0
9	1219.0
10	2315.0
11	5904.5
12	9494.0
13	11629.0
14	13764.0
15	11036.5
16	8309.0
17	6937.0
18	5565.0
19	5183.0
20	4801.0
21	5447.0
22	6093.0
23	5876.5
24	5660.0
25	5677.5
26	5695.0
27	5962.0
28	6229.0
29	6798.5
30	7368.0
31	7242.0
32	7116.0
33	7745.0
34	8374.0
35	8581.5
36	8789.0
37	9101.0
38	9413.0
39	10114.0
40	10815.0
41	9396.0
42	7977.0
43	8805.0
44	9633.0
45	8964.5
46	8296.0
47	8876.5
48	9457.0
49	9468.5
50	9480.0
51	9325.0
52	9170.0
53	9714.0
54	10258.0
55	9799.5
56	9341.0
57	10302.5
58	11264.0
59	8935.5
60	6607.0
61	7163.5
62	7720.0
63	6133.5
64	4547.0
65	3544.0
66	2541.0
67	1661.0
68	781.0
69	769.0
70	757.0
71	928.0
72	1099.0
73	1037.0
74	975.0
75	505.0
76	35.0
77	31.5
78	28.0
79	185.5
80	343.0
81	172.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	230241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.195560304202987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6893893036348	11.028444108564504
2	7.431770897075022	2.109963038728984
3	2.410965610084445	1.0267502312793986
4	1.070860359809081	0.6080585126020127
5	0.6333374128013708	0.44952897181648793
6	0.4711785583159956	0.4013186183173284
7	0.4099865377554767	0.4073992034433485
8	0.2600660873822054	0.29534270612097757
9	0.2906620976624648	0.37135002019622915
>10	6.526128992779341	22.709682463158167
>50	1.4441316852282462	14.387098735672621
>100	1.3125688410231307	39.58070022281001
>500	0.03977481336433729	3.973228052345151
>1k	0.009178803084077836	2.651135114944775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2595	1.1270798858587305	No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2313	1.0045995283203253	No Hit
TCAAAGGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAAC	1196	0.5194557007657195	No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	928	0.40305592835333415	No Hit
TCAAAGGGGACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC	848	0.3683097276332191	No Hit
TCAAAGTGGCTAATTCTCTTTCCTGTACTTTAATAGACTGACAGCCTCAT	817	0.35484557485417456	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	747	0.3244426492240739	No Hit
TCAAAGGGGTCATCTCCTCATTACAATTAAATTAGCTTTGCCAGATAAGA	729	0.31662475406204804	No Hit
TCAAAGGGGACAAGCCCTTTGGGGGGATGCTGCAGAAATCTGGCACTCCC	728	0.3161904265530466	No Hit
TCAAAGGGGAGCTTTGTTGCAGGTGTTTCTACCAGTGCTGCGGATTTGGG	718	0.3118471514630322	No Hit
TCAAAGGGGGTAAGGAAGTCACTGATGTGTGTATTTTTGTACTGAGAGTA	681	0.29577703362997904	No Hit
TCAAAGGGGAACTCAGGAATTTGTAGAGGCAGAAGCAATAGAAGATAGAG	642	0.27883826077892293	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	614	0.2666770905268827	No Hit
TCAAAGGGGGGATGGAGCCACCCTTCCCCCTGCCCTGAACAGCCAGGGTG	596	0.25885919536485685	No Hit
TCAAAGGGGAGTGCAGTGCTTCAAAGGGGACCTGGAGGTCTTTGCCCCTA	575	0.2497383176758266	No Hit
TCAAAGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT	525	0.22802194222575475	No Hit
TCAAAGGGGGAGGGGGGAGGGGCAGGGCCGCAGGGTGTCATGGGAGAGGG	494	0.2145577894467102	No Hit
TCAAAGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAA	489	0.21238615190170299	No Hit
TCAAAGGGGAGCTGCCAAAGCTTAGGCACTCTCTACACTCTTGTGTAAAA	459	0.1993563266316599	No Hit
TCAAAGGGGTGCCTACTGTATTGCTTTGTGCTCTCACCTCTGATTTCACT	456	0.19805334410465555	No Hit
TCAAAGGGGGGTGGGGCCTGGACCTACAACACCACCTCTGCAGTCACAGT	450	0.19544737905064694	No Hit
TCAAAGGGGACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCT	443	0.19240708648763685	No Hit
TCAAAGGGGCTCCACCATCCGAAGAGATGCCCCTGCTGGGCGCAAAGTGG	428	0.1858921738526153	No Hit
TCAAAGGGGACTAATCGGACTGCCAAATTTCACCGGTTTGCCCTGGGATA	415	0.1802459162355966	No Hit
TCAAAGGGGCGTACACCTGTAATCTCAGCCACTCAGGAGGCTGAGGCAGG	414	0.17981158872659517	No Hit
TCAAAGGGGGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGG	413	0.17937726121759373	No Hit
TCAAAGGGGACCTGGGGAAGCTTTGCATACCCAGCCTAGACCGATCAGAT	410	0.17807427869058942	No Hit
TCAAAGGGGATTGGTAAAAGACTGTATAGCATCTATTTATTTAGATGATT	404	0.1754683136365808	No Hit
TCAAAGGGGAAGGCAACTGTGGCCAATATTTATATCATGACATACAAATG	395	0.17155936605556785	No Hit
TCAAAGGGGCTGATGCCCTGTGTGTGTGTGTGTGTGTGTATTTGGGGCTG	395	0.17155936605556785	No Hit
TCAAAGGGGATTGGGAACCCATTTTCCACCTGGTCTTTCTTGACAGGGTT	391	0.1698220560195621	No Hit
TCAAAGGGGGGACAGTAGACTGTTCTGTAATTAAAATGTAGTATACTGCT	389	0.16895340100155926	No Hit
TCAAAGGGATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGAC	388	0.1685190734925578	No Hit
TCAAAGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG	387	0.16808474598355636	No Hit
TCAAAGGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	384	0.16678176345655205	No Hit
TCAAAGGGGCCTGTCCAAATTCTTCCTACTGGTCAATGCCCATCCCAAGT	382	0.16591310843854917	No Hit
TCAAAGGGGACGTACCCCGTACCCATAGCTTAGTTTTCTGGGAGCAGAAT	382	0.16591310843854917	No Hit
TCAAAGGGGGCGAGGCGGAGTCCCGCTCCCGTGGGGAGGGGTGTGTACAT	380	0.1650444534205463	No Hit
TCAAAGGGGAGCGACTGGATGCTCGCCATCAAAGTCCAGTGGAAATTCTT	367	0.1593981958035276	No Hit
TCAAAGGGGAAGGACAAAGTGGTAAAAAAGGAAATAAAGACAAAACTCGA	367	0.1593981958035276	No Hit
TCAAAGGGGACTGTGGGGATAACATACACTGTAATGGGGGAAAATTACCT	366	0.15896386829452616	No Hit
TCAAAGGGAGGCTTTTTTTAAAAAAAAAGTGTTTCTGTGCCATTGTTCGT	366	0.15896386829452616	No Hit
TCAAAGGGGAGCTGGAGCTGCGCCTGCTGGTGGTGCTGCTCCATCCACTG	366	0.15896386829452616	No Hit
TCAAAGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	360	0.15635790324051754	No Hit
TCAAAGGGGATAGTCATGATGGTCCGGATGCGGGTACCGTGCTTCACGGA	358	0.15548924822251467	No Hit
TCAAAGGGGTACAGATTCTGGGGCCTCGGGGCCTATGTTCTGAACCGTTA	354	0.15375193818650892	No Hit
TCAAAGGGGAGACAGAGTACTTGGAGGAGAGGAGGGTCAAGGAAGTGGTG	351	0.15244895565950461	No Hit
TCAAAGGGGATTCTAACCACCTACAGCTGGCCCCGAGCCAAGCCTTCCAG	346	0.15027731811449743	No Hit
TCAAAGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	343	0.1489743355874931	No Hit
TCAAAGGGGCACTGGGGCGCAGCAGCGCGCTGCCCCTCTGCCCCAGGGAC	340	0.14767135306048879	No Hit
TCAAAGGGGACGGTCTCTTTGGTTTCTGTCTGTAAGTTACGGCCCTGGAT	339	0.14723702555148735	No Hit
TCAAAGGGCTTCGCTCGCTCAACCTCTTTTGTTCAGTACGTGTAACTTGA	339	0.14723702555148735	No Hit
TCAAAGGGGACTCAAGAATGACCCCATGGTCTGTGGGGTGGGCCAGAGTG	338	0.1468026980424859	No Hit
TCAAAGGGGATCTTCCCTCCCCTTTGTCTCTGAGAGAGTGCTTCGCGCAC	337	0.14636837053348448	No Hit
TCAAAGGGGATTTGAACATCATTTTAATTATTACAGGACAATGATAAGTG	335	0.1454997155154816	No Hit
TCAAAGGGGAACCTTGCGTGCCATTCGGTCCCATTCCCATCTGCTCACTA	334	0.14506538800648017	No Hit
TCAAAGGGGGGAATTTCGATTCTGTGGATAGGGAAGTGGGATTGGTATCT	334	0.14506538800648017	No Hit
TCAAAGGGGCGTGGGCCCCGTCTCCACCCCTGGCCCTGCCCAGCCTGTGT	332	0.1441967329884773	No Hit
TCAAAGGGGCCCCACAGCATGAGGGATCTACCTAGACCAGGGATCGAACC	331	0.14376240547947586	No Hit
TCAAAGGGGGATCATTGTTTGTGATTGCATGTTTCCTTCAACTGTGTTCT	328	0.14245942295247155	No Hit
TCAAAGGGGGACGCACGCCTTTAATCAGAGCACTCGGGAGGCAGAGGCAG	328	0.14245942295247155	No Hit
TCAAAGGGGAAGCGAGTGAGTGAGTTGAGTTACCTGAACTCTGTAGCACT	328	0.14245942295247155	No Hit
TCAAAGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	327	0.1420250954434701	No Hit
TCAAAGGGGGAGAAACATTCATAGCTGCAAAAACCATCATAAGTAGAGGA	327	0.1420250954434701	No Hit
TCAAAGGGGAGCGGGCAAGGTTTATACGTGCCTTTTTGTTTTTGTAAAAA	326	0.14159076793446868	No Hit
TCAAAGGGGCTTAGGAAATGTGCAAATGAGATCCAACTCCAATTCAACAA	325	0.1411564404254672	No Hit
TCAAAGGGGACCCACCAAGGAGACAGACATAGCCAGACTCAACTCTGGAA	324	0.14072211291646577	No Hit
TCAAAGGGGGCCGAACCCACGTCGGTAGAATACGAGCGACCGGCAGGGCT	323	0.14028778540746437	No Hit
TCAAAGGGGATTCGGTCTGCATTCCCAGTGGGCAGTGATGGCATTACTCT	321	0.13941913038946147	No Hit
TCAAAGGGGAGCGGTATGGTTCGTGCCAAATTCCGAAGCAACCTTCCTGC	321	0.13941913038946147	No Hit
TCAAAGGGGTACAGGGTTGTTGATTTGTTCACAAAGGAAACTGCCCAGAA	321	0.13941913038946147	No Hit
TCAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	318	0.13811614786245718	No Hit
TCAAAGGGGGTCGAGCCCAGTGTTACCACCAAGAAGGTCAAACAGGAAGA	317	0.13768182035345572	No Hit
TCAAAGGGGCTCTGGGAACCCAGGGTCCCCAGATTTTCTGAGACGGAAGT	316	0.13724749284445428	No Hit
TCAAAGGGGCAGAGTGCAGTGCTTCAAAGGGGGACGATAACTGATAGTGC	316	0.13724749284445428	No Hit
TCAAAGGGGAGCGTTTGGATGGATGAGGTGTAGGAAAGCTGTGGAGCAGC	314	0.1363788378264514	No Hit
TCAAAGGGGGCTTTCACATCCTTTGCACCCTACCCCCATCATGATGTTGT	312	0.13551018280844854	No Hit
TCAAAGGGGAGGGCCACTCTAGAAAGGCCAACAGCCTTCCGGTGGCTTCT	311	0.1350758552994471	No Hit
TCAAAGGGGCAGAGTGCTGTGCTTCAAAGGGGATCCCACAGTTGTAGTTT	311	0.1350758552994471	No Hit
TCAAAGGGGATGAATACTTACGTTTACCTCACTGGTGCATTTAAAATGGA	309	0.13420720028144423	No Hit
TCAAAGGGGATATCCCGGGGAGAAGATGGGCTGTCCCCACCCACGGGGCC	309	0.13420720028144423	No Hit
TCAAAGGGATGAAGGCTCTTGGCATTCCTTTGTAGATAGGAAGTTCCTTG	307	0.13333854526344135	No Hit
TCAAAGGGGGTCTCCCAGCCTTGGGGCCCCCGGAGCCTCCCCTCTTCTCC	303	0.1316012352274356	No Hit
TCAAAGGGGAGTTGTGGGTAGGAAGAAAGTTAATGTAGCCCTGTGGTTTT	301	0.1307325802094327	No Hit
TCAAAGGGGATGAGTGACAGGACGGTGCTGGTTTTCTACCTGCTTACTCT	300	0.13029825270043127	No Hit
TCAAAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	298	0.12942959768242843	No Hit
TCAAAGGGGCTACTGGGTTGGTGAAGATTCCACATATAAATTTTTTGAGG	298	0.12942959768242843	No Hit
TCAAAGGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	297	0.12899527017342696	No Hit
TCAAAGGGGGCATAGGTGTGACCCATAGCTTTCTTGTCCTTGTAAGAGGT	296	0.12856094266442553	No Hit
TCAAAGGGGCTCTGACAGTCTGGTCACCGGTCCTGGCCCACCCTCCCCTT	295	0.12812661515542412	No Hit
TCAAAGGGGTGTAGGCCCCACCACATCCACCACATGGTCCCCAGCCAGTA	295	0.12812661515542412	No Hit
TCAAAGGGGGTCTTACAACTGAACATGTATGTGTGTTGCTTAGATAAATG	292	0.12682363262841978	No Hit
TCAAAGGGGAGGGAACAAAGCATCATAAATAAATCAGGAGTGAAAAAGAG	292	0.12682363262841978	No Hit
TCAAAGGGGAGTTGGATCTTGGGAGCGTCCCCGCCCCTTGCCTCTCGGCG	292	0.12682363262841978	No Hit
TCAAAGGGGAATTCTTTACTGTGGAATAGCTTGGGACATCAGCTCTGTTT	292	0.12682363262841978	No Hit
TCAAAGGGGGTGGGTGGTTGGATAACTCTAGATGACTCGGGGTGCATACC	291	0.12638930511941834	No Hit
TCAAAGGGGATGTTTTTTGTGTGTCAATTGTGCCATTTTTATTTTATTCC	290	0.1259549776104169	No Hit
TCAAAGGGGACTTGTCTGAGGGTAAAGGGACCGCTTTGTCACAAATGCTA	290	0.1259549776104169	No Hit
TCAAAGGGGGAAAAGCCTGGAGGAGAAAACTTGGAATTTTTGTAATTCTT	289	0.12552065010141547	No Hit
TCAAAGGGGGTGCAGTTCTTCAAAGGGGTGTTATTAATACTGAACTCCAT	289	0.12552065010141547	No Hit
TCAAAGGGGAGTTGTGCCAAATTTGGGTCAGAGCGCAAATCAGAAGGTTA	289	0.12552065010141547	No Hit
TCAAAGGGGGACAGTTGTGTGACTTTGTGTGGTCATGTTATGGATCTTAC	287	0.1246519950834126	No Hit
TCAAAGGGGAGTTTGTGTCCCTGGGCCTGCCCCCATCGGCCCTGCTGATG	286	0.12421766757441118	No Hit
TCAAAGGGGAGCTGGATGGTGTGGATGATGGGACAGAGAGTGACACCAGT	286	0.12421766757441118	No Hit
TCAAAGGGGGACCCGTCAATCATGGGCGGGATGATTGTCCGAATTGGGGA	286	0.12421766757441118	No Hit
TCAAAGGGGCAGCCTCCTGAGAGTCTCAAGCTCGAAGCTGGAGACAAGAA	286	0.12421766757441118	No Hit
TCAAAGGGGAGTCTAAGTACCAATGCTATTGTAAACAACGTGTATAGTGC	286	0.12421766757441118	No Hit
TCAAAGGGGAGGGATTCATAGAGTTCCGGGCCTGGGATTCTCCCAGCTGC	285	0.12378334006540971	No Hit
TCAAAGGGGGTGCTGACTTTCCATGTGACCATTAGACTTTAAGGGATATA	285	0.12378334006540971	No Hit
TCAAAGGGGGTTCCTGCCCTTGTGCCTTAGTTCAGTTCCTACCAGAGAAG	285	0.12378334006540971	No Hit
TCAAAGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	285	0.12378334006540971	No Hit
TCAAAGGGGATTCTGGGACAGTGCTGGGAGCATGGTAGGAGTGTGCAGCA	283	0.12291468504740685	No Hit
TCAAAGGGGAGTTGGAGAGTGCAGGCCCCTGAGCAAGACTGTGCGATTCA	282	0.12248035753840542	No Hit
TCAAAGGGGATTTGCACTTTGAAGAATGTAGAGAGTAAAAAAACTAAAAT	282	0.12248035753840542	No Hit
TCAAAGGGGGGAAGTACCAGGCAGTGACAGCCACTCTGGAGGAGAAACGG	281	0.12204603002940397	No Hit
TCAAAGGGGGATCCACTAAGGAAATGTTTTATCAGCTAGAGACCACTGCT	279	0.1211773750114011	No Hit
TCAAAGGGGCAGGAGCTCCTTAGTAAAGGACTTATCAAGCTGGTTTCAAA	276	0.11987439248439678	No Hit
TCAAAGGGGGAAGACTTATACCATTGTGTTTAAGCTGTATTGAATTATCT	276	0.11987439248439678	No Hit
TCAAAGGGGGTTTTTAAAATATGAATGCATACTTGTAATATATTTTTTAA	276	0.11987439248439678	No Hit
TCAAAGGGGATGGTCCCCTATTCAGACACTAGGCCAGGTGTGGCTGGGGC	275	0.11944006497539535	No Hit
TCAAAGGGGCAAGGGAATCAAGAGCCCTGGGCTGAGCTGTGCTCTGCCAG	274	0.1190057374663939	No Hit
TCAAAGGGGATGGCCACCCCCACTCCATGTGAGTCCACTTGGCTCTGTCC	274	0.1190057374663939	No Hit
TCAAAGGGGGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGT	272	0.11813708244839104	No Hit
TCAAAGGGGGCATGAAGGCCGATGGTAACCTGGTGTACATTAAAATAAGT	272	0.11813708244839104	No Hit
TCAAAGGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACG	272	0.11813708244839104	No Hit
TCAAAGGGGGTACATAAGTAACACGAGATGCTCTGTATGTGTGAGTGCGT	272	0.11813708244839104	No Hit
TCAAAGGGGGTCCTCAGCCCCCTGAAGCTCTGAGAGGCCCTGTCTCGGCA	272	0.11813708244839104	No Hit
TCAAAGGGGAGTGCAGTGCTTCAAAGGGGAGCCATGCTCGATGCGCTTCA	271	0.1177027549393896	No Hit
TCAAAGGGGATTGTAGCTACCACATTGGTTTTTCTATAATCACGCGGGGT	271	0.1177027549393896	No Hit
TCAAAGGGGGTGAGGGAGGTGGGGAGGGCATGGTTTATGTTGAATGTTTA	270	0.11726842743038815	No Hit
TCAAAGGGGAGGGTTTTGGTTTTTGTTTTGTTTTGGTTTTGTACTAAGAA	268	0.11639977241238528	No Hit
TCAAAGGGGTATATCTACAGCTAAGTATCACGTTACTACATAGCACCACA	268	0.11639977241238528	No Hit
TCAAAGGGGGTTCCTAACTCCTTTTTGTTATACACCCCATCCATGATCAT	267	0.11596544490338385	No Hit
TCAAAGGGGAGCTAATCAGATCTGCCTCAGGAAAAATACTAACTTGTTCT	265	0.11509678988538097	No Hit
TCAAAGGGAGTGCAGTGCTTCAAGGGGTACCCTGCGGTTCCTGCCTCAAC	261	0.11335947984937522	No Hit
TCAAAGGGGAAATGGGACTGATGTCATCTTGAGCTTTTATTTTGACCGTG	259	0.11249082483137235	No Hit
TCAAAGGGGCTTTCCTGAGCCAGAGTTCCAGGAACACTGTGGACATCTTT	259	0.11249082483137235	No Hit
TCAAAGGGGGACGGCTCCAGTGGAGGGGTGATCCGCTTGGCAGCCATTCA	258	0.1120564973223709	No Hit
TCAAAGGGGGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCT	258	0.1120564973223709	No Hit
TCAAAGGGGGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTTGTCCCCC	257	0.11162216981336946	No Hit
TCAAAGGGGACTGTTTTCTCTGTCTGCTGTCTAACCCTGTGCCTTGCCTG	256	0.11118784230436804	No Hit
TCAAAGGGGAGATGGTTGGGTACGTGCTTGTGGCTGAGGAAGGGCGGGTG	256	0.11118784230436804	No Hit
TCAAAGAGGGAAGCTGAAGATGACAACCTTGATGACCTAGAGAGGCACCT	256	0.11118784230436804	No Hit
TCAAAGGGGGAGGCAAAGTTGGGCCAGCTGTTTCTGGGTGTGCAGTTAGA	255	0.1107535147953666	No Hit
TCAAAGGGGGTCTTGTATTTGTTTTGTTCTGTTTTCTCATTGGGGGTAAA	254	0.11031918728636515	No Hit
TCAAAGGGGATTATTTAGACTTGTATTTAGAACGTGTCACTTCCTCGAGC	254	0.11031918728636515	No Hit
TCAAAGGGGGGGGTAAGGGATATGGCTAAACTAGGAATCTGGAATTAAAA	254	0.11031918728636515	No Hit
TCAAAGGGGAGCTAAAACATTGTTAAAAAAAAATAAAATTGACTTTGAGT	253	0.10988485977736372	No Hit
TCAAAGGGGGAAGAAGCATTCTCAGTTCATTGGCTATCCCATTACTCTCT	252	0.10945053226836228	No Hit
TCAAAGGGGTGCAGTGCTTCAAAGGGGGGGCTGGTGAGATGGCTCAGTGG	252	0.10945053226836228	No Hit
TCAAAGGGGATTCTGTTTGGTGGCTGATAAAGAGACTTTGAGATTACAAA	251	0.10901620475936086	No Hit
TCAAAGGGGAGCCACAAGACAGCCTTTACTGAAAAAGCTATTGGACTCTT	251	0.10901620475936086	No Hit
TCAAAGGGGAATGAAGCTCTGAAATAAAATGGGACCGTTGAGATGACTTC	251	0.10901620475936086	No Hit
TCAAAGGGGTAATACAGAGATAAAGCAAGAAACAAAAGGGAAAAAATGCA	251	0.10901620475936086	No Hit
TCAAAGGGGATCCAATGTATGTCAGCCATTGTCTCTGCTGGCTTGTGACT	250	0.1085818772503594	No Hit
TCAAAGGGATGTAGAATCACCTTTGATTTCATGGCAATTGACAGTCCTAA	250	0.1085818772503594	No Hit
TCAAAGGGGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	250	0.1085818772503594	No Hit
TCAAAGGGGATATCCTCACCGCCCCAGCCTGGTCCCAGGCATGGCTGACT	249	0.10814754974135797	No Hit
TCAAAGGGAATTAAAAAAAAGCATATTTGTCACGGAAATTTAGTTACCGT	249	0.10814754974135797	No Hit
TCAAAGGGGGATAGCTTTACCTGGCCCAGCATCAAAGCTGTTGACAAGCA	249	0.10814754974135797	No Hit
TCAAAGGGGGTGCTTCAAAGGGGAGCCATACTCTGCTATGTAATGTTATA	248	0.10771322223235653	No Hit
TCAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	245	0.10641023970535221	No Hit
TCAAAGGGGCATGCCTGCACCTGGGTCGGGGTGCTCTGGGACTGGGAGAT	245	0.10641023970535221	No Hit
TCAAAGGGGAGCGCGGCGGCGGAAACCGCAGCCCGGCGGCCCTGGAACCT	244	0.10597591219635079	No Hit
TCAAAGGGGGATGTTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACG	243	0.10554158468734935	No Hit
TCAAAGGGGACAGCTATGGGACAGCAGCCATGGGCTGTGTCTACATGGGC	243	0.10554158468734935	No Hit
TCAAAGGGGGCATATTTACTCTTCGTCAGTGCTATCTTCTTGTATGTCAT	243	0.10554158468734935	No Hit
TCAAAGGGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	243	0.10554158468734935	No Hit
TCAAAGGGGAGAATATACATACCTGTAGGATGCTGTGTAATGGGAAACCA	241	0.10467292966934647	No Hit
TCAAAGGGGAACGAGGGCCGCGAAAGCGCGGTTATGAATATTAATGAGGC	241	0.10467292966934647	No Hit
TCAAAGGGGGGTGAGGGAAGAAAACACACTCAACACCAAAGGGTCATCTT	239	0.1038042746513436	No Hit
TCAAAGGGGAATCATAACAGACAACACAGAAATACAAAGGATCATAAAAC	239	0.1038042746513436	No Hit
TCAAAGGGGCAGAGTGCAGTGCTTCAAAGGGGACGCTCCAAGGATGTATA	238	0.10336994714234214	No Hit
TCAAAGGGGATGCCACAGAGCTGTCAGAATGCAGCACAGCTCCAGCAGCC	238	0.10336994714234214	No Hit
TCAAAGGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	238	0.10336994714234214	No Hit
TCAAAGGGGGGGTACCCAAGCACCCCAGCCCTCCTGCCTACCTCAGTCAT	237	0.10293561963334072	No Hit
TCAAAGGGGATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	236	0.10250129212433928	No Hit
TCAAAGGGTGACCCATTACAAGCACGGAAATTGAAACTGTAATCAAAAAT	236	0.10250129212433928	No Hit
TCAAAGGGGGTTTTGTATCAGTTCTTTTCTTTGTAAATGGCATTAACATT	235	0.10206696461533785	No Hit
TCAAAGGGGAGGCCCTTCCAAGGGAAATCTCTGCCCTCTGCATCCTCAGC	234	0.1016326371063364	No Hit
TCAAAGGGGGAGTATTAGTTGCAGGCACTGATGCTTCTGTGCTGTTTCCA	233	0.10119830959733496	No Hit
TCAAAGGGGGGAAGCTCAAGATGACAGCATTCTCAAGTGAGGCGTCAGCG	233	0.10119830959733496	No Hit
TCAAAGGGGAGATTCACACTGCTGACGTGTCTTGCAGCGTAGGTGGGTTG	233	0.10119830959733496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.07383567653024439	0.0	0.0	0.0	0.0
6	0.07383567653024439	0.0	0.0	0.0	0.0
7	0.0751386590572487	0.0	0.0	0.0	0.0
8	0.07687596909325446	0.0	0.0	0.0	0.0
9	0.07948193414726308	0.0	0.0	0.0	0.0
10	0.1281266151554241	0.0	0.0	0.0	0.0
11	0.16287281587553912	0.0	0.0	0.0	0.0
12	0.18111457125359948	0.0	0.0	0.0	0.0
13	0.19805334410465555	0.0	0.0	0.0	0.0
14	0.22454732215374326	0.0	0.0	0.0	0.0
15	0.25321293774783815	0.0	0.0	0.0	0.0
16	0.2792725882879244	0.0	0.0	0.0	0.0
17	0.30185761875599915	0.0	0.0	0.0	0.0
18	0.3179277365890523	0.0	0.0	0.0	0.0
19	0.34311873211113575	0.0	0.0	0.0	0.0
20	0.3626634700162004	0.0	0.0	0.0	0.0
21	0.38307686293926796	0.0	0.0	0.0	0.0
22	0.40392458337133696	0.0	0.0	0.0	0.0
23	0.42998423391142326	0.0	0.0	0.0	0.0
24	0.4582155219965167	0.0	0.0	0.0	0.0
25	0.5007796178786575	0.0	0.0	0.0	0.0
26	0.5376974561437797	0.0	0.0	0.0	0.0
27	0.5733123118818977	0.0	0.0	0.0	0.0
28	0.6219569928900587	0.0	0.0	0.0	0.0
29	0.6614807962091895	0.0	0.0	0.0	0.0
30	0.7057822021273361	0.0	0.0	0.0	0.0
31	0.7487806255184785	0.0	0.0	0.0	0.0
32	0.7856984637836006	0.0	0.0	0.0	0.0
33	0.8234849570667258	0.0	0.0	0.0	0.0
34	0.8621401053678537	0.0	0.0	0.0	0.0
35	0.902098236195986	0.0	0.0	0.0	0.0
36	0.9394504019701095	0.0	0.0	0.0	0.0
37	0.9685503450732059	0.0	0.0	0.0	0.0
38	0.9946099956132921	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCT	65	0.0	44.000004	31
TCATTAC	65	0.0	44.000004	18
ATGGAGC	65	0.0	44.000004	12
GGACTAA	65	0.0	44.000004	8
GGACGTA	65	0.0	44.000004	8
AATTAGC	65	0.0	44.000004	30
AGGGGTC	130	0.0	44.000004	5
TGATGCC	65	0.0	44.000004	11
CCCTGAT	25	4.431719E-5	44.0	41
CCTTCGG	20	7.8433525E-4	44.0	24
CCTTCCG	25	4.431719E-5	44.0	35
GTGTTCG	30	2.5188692E-6	44.0	21
GCCCCCG	25	4.431719E-5	44.0	26
CTTCGGT	20	7.8433525E-4	44.0	25
CTTCGGA	30	2.5188692E-6	44.0	21
ACAACCT	20	7.8433525E-4	44.0	20
ATCCTCC	25	4.431719E-5	44.0	44
AGCGTTT	50	2.7284841E-11	44.0	10
ATCCTAG	20	7.8433525E-4	44.0	13
CTATGTT	25	4.431719E-5	44.0	33
>>END_MODULE