##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527461_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179718
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12347678028912	33.0	31.0	34.0	31.0	34.0
2	32.52535082740738	34.0	31.0	34.0	31.0	34.0
3	32.94371737945003	34.0	31.0	34.0	31.0	34.0
4	36.337551052204006	37.0	37.0	37.0	35.0	37.0
5	36.31193870396955	37.0	37.0	37.0	35.0	37.0
6	36.38317252584605	37.0	37.0	37.0	35.0	37.0
7	36.66707842286248	37.0	37.0	37.0	35.0	37.0
8	36.740537953905566	37.0	37.0	37.0	35.0	37.0
9	38.67957577983285	39.0	39.0	39.0	39.0	39.0
10	36.98273406114023	39.0	37.0	39.0	34.0	39.0
11	36.97089328837401	39.0	35.0	39.0	34.0	39.0
12	37.225497724212374	39.0	37.0	39.0	34.0	39.0
13	37.37646757698172	39.0	37.0	39.0	35.0	39.0
14	38.129686508863884	40.0	38.0	40.0	35.0	40.0
15	38.26400249279427	40.0	38.0	40.0	35.0	40.0
16	38.456120143780815	40.0	38.0	40.0	35.0	40.0
17	38.454295062264215	40.0	38.0	40.0	35.0	40.0
18	38.331118752712584	40.0	38.0	40.0	35.0	40.0
19	38.28926429183499	40.0	38.0	40.0	35.0	40.0
20	38.324552910671166	40.0	38.0	40.0	35.0	40.0
21	38.26999521472529	40.0	38.0	40.0	35.0	40.0
22	38.245156300426224	40.0	38.0	40.0	35.0	40.0
23	38.19459931670728	40.0	38.0	40.0	35.0	40.0
24	38.06292079813931	40.0	37.0	40.0	35.0	40.0
25	38.01707118930769	40.0	37.0	40.0	35.0	40.0
26	37.91260752957411	40.0	37.0	40.0	35.0	40.0
27	37.86720306257581	40.0	36.0	40.0	35.0	40.0
28	37.70521038515897	40.0	35.0	40.0	34.0	40.0
29	37.53180538399047	39.0	35.0	40.0	34.0	40.0
30	37.34524087737455	39.0	35.0	40.0	34.0	40.0
31	37.20731924459431	39.0	35.0	40.0	34.0	40.0
32	37.08325821564896	39.0	35.0	40.0	34.0	40.0
33	36.94280483869173	39.0	35.0	40.0	34.0	40.0
34	36.70970631767547	38.0	35.0	40.0	33.0	40.0
35	36.615258349191514	38.0	35.0	40.0	33.0	40.0
36	36.43811415662315	38.0	35.0	40.0	33.0	40.0
37	36.30527270501563	38.0	35.0	40.0	33.0	40.0
38	36.412078923647044	38.0	35.0	40.0	33.0	40.0
39	36.2676637843733	38.0	35.0	40.0	33.0	40.0
40	36.163483902558454	38.0	35.0	40.0	33.0	40.0
41	36.07602466085756	38.0	35.0	40.0	33.0	40.0
42	35.97147753703024	38.0	35.0	40.0	33.0	40.0
43	35.91564005831358	38.0	35.0	40.0	33.0	40.0
44	35.864459876028	37.0	35.0	40.0	33.0	40.0
45	35.74433278803459	37.0	35.0	40.0	32.0	40.0
46	35.58020899409074	37.0	35.0	40.0	31.0	40.0
47	35.487753035310874	37.0	35.0	40.0	31.0	40.0
48	35.30656918060517	36.0	35.0	40.0	31.0	40.0
49	35.064295173549674	35.0	35.0	40.0	30.0	40.0
50	35.230249613283036	36.0	35.0	40.0	31.0	40.0
51	35.19983529752167	36.0	35.0	40.0	31.0	40.0
52	34.85441079914088	35.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	8.0
16	8.0
17	11.0
18	22.0
19	43.0
20	84.0
21	123.0
22	217.0
23	317.0
24	453.0
25	739.0
26	1068.0
27	1398.0
28	1648.0
29	1909.0
30	2069.0
31	2568.0
32	3481.0
33	5634.0
34	12791.0
35	12220.0
36	28085.0
37	26759.0
38	60676.0
39	17383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.2032962752757	0.5458551731045304	1.108403164958435	0.14244538666132497
8	98.92887746358183	0.7010983874737089	0.1736053149934898	0.19641883395096765
9	96.27360642784807	2.6547146084421147	0.4418032695667657	0.6298756941430463
10	37.91885064378638	48.7875449315038	5.160863130014802	8.132741294695023
11	28.271514261231484	27.96380996895136	31.004685117795656	12.7599906520215
12	25.88221547090442	24.127800220345208	28.428983184767247	21.56100112398313
13	24.047674690348213	25.661870263412677	31.70411422339443	18.58634082284468
14	25.635718180705325	30.129424988036813	25.992944501941935	18.24191232931593
15	21.849230461055654	34.600874703702466	26.489277646089988	17.060617189151895
16	23.461756752245183	37.561624322549775	22.465195472907556	16.51142345229749
17	23.142367486840495	36.827696724868964	21.290021032951625	18.739914755338923
18	19.677494741762093	38.66724535104998	23.140698204965556	18.51456170222237
19	19.03537764720284	39.9759623410009	25.026430296353176	15.962229715443083
20	22.39675491603512	37.26727428526914	24.867848518234123	15.468122280461612
21	21.66115803647937	39.745601442259535	24.064367509097586	14.528873012163501
22	18.254710157023784	39.33384524644165	25.20337417509654	17.20807042143803
23	17.271503132685652	41.77544820218342	25.450984319878923	15.502064345252005
24	17.105687799775204	44.82411333311076	22.198110372917572	15.872088494196463
25	19.92454845925283	44.62825092645144	20.73526302318076	14.711937591114967
26	18.25749229348201	45.70271202661948	20.153796503410902	15.885999176487609
27	17.814576169331954	43.61221469190621	21.638900944813543	16.934308193948297
28	17.952013710368465	44.45575846604124	23.204130916213177	14.388096907377113
29	19.7030903971778	40.9369122736732	22.138016225419825	17.221981103729174
30	19.711993233844133	40.683181428682715	22.476880446032116	17.127944891441036
31	18.50009459263958	42.78369445464561	22.467977609365782	16.248233343349025
32	19.802690882382397	42.916124150057314	23.006042800387274	14.275142167173016
33	19.009225564495488	43.98168241355902	20.83987135401017	16.16922066793532
34	17.91862807286972	44.242646813340905	20.98676815900466	16.85195695478472
35	17.614262344339465	45.664874970787565	21.516486940651465	15.204375744221501
36	18.123393316195372	45.364960660590484	20.807598571094715	15.704047452119433
37	17.896370981203884	44.99438008435438	24.113333110762415	12.995915823679319
38	19.42877174239642	43.94050679397723	20.70132095839037	15.92940050523598
39	17.06506860748506	44.31331307937992	21.717357192935598	16.904261120199422
40	19.903960649461936	43.504267797326925	21.321737388575436	15.270034164635707
41	16.540914098754715	44.89756173560801	21.66115803647937	16.900366129157902
42	18.751599728463482	45.60589367787311	19.53226721864254	16.110239375020864
43	18.053283477448	47.31468189051737	20.988993868171246	13.643040763863388
44	18.70541626325688	46.473920252840564	19.43656172447946	15.384101759423096
45	18.588010104719615	47.24234634260341	18.72656050033942	15.44308305233755
46	18.1395297076531	47.519447133843016	20.14990151236938	14.1911216461345
47	18.486740337640082	49.37568857877341	17.736119921209895	14.401451162376613
48	17.166338374564596	49.652789370013025	18.117272615987268	15.063599639435116
49	18.412179080559543	46.19681946160095	18.44723399993323	16.943767457906276
50	15.67121824191233	48.19161130215115	20.66793532089162	15.469235135044904
51	19.35587976719082	47.291311944268244	19.25683570927787	14.095972579263066
52	17.753369167250916	47.215637832604415	20.51547424298067	14.515518757164001
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.5
6	2.0
7	59.5
8	117.0
9	961.5
10	1806.0
11	4571.5
12	7337.0
13	8871.0
14	8151.5
15	5898.0
16	5000.0
17	4102.0
18	3868.5
19	3635.0
20	3932.5
21	4230.0
22	4032.5
23	3835.0
24	3690.5
25	3546.0
26	4179.0
27	4812.0
28	4753.0
29	4694.0
30	5140.5
31	5587.0
32	6233.0
33	6879.0
34	6023.5
35	5168.0
36	5953.5
37	6739.0
38	7116.5
39	7963.5
40	8433.0
41	7011.5
42	5590.0
43	5900.0
44	6210.0
45	7337.0
46	8464.0
47	7169.0
48	5874.0
49	6416.5
50	6959.0
51	7214.0
52	7469.0
53	7986.5
54	8504.0
55	7376.0
56	6248.0
57	6160.5
58	6073.0
59	6578.0
60	7083.0
61	6598.0
62	6113.0
63	4916.5
64	3391.0
65	3062.0
66	2129.5
67	1197.0
68	731.0
69	265.0
70	676.0
71	1087.0
72	809.0
73	531.0
74	413.0
75	295.0
76	157.5
77	20.0
78	57.5
79	95.0
80	114.0
81	133.0
82	68.5
83	4.0
84	3.0
85	2.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	179718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.905218175141053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6906394170655	9.638989973180205
2	6.976242831888932	1.800598715765811
3	2.1170180657957145	0.8196174005942642
4	1.1684559996550683	0.6031671841440479
5	0.7286681326262234	0.47018106144070154
6	0.5475790109084637	0.4239975962341001
7	0.5518906566636484	0.49855885331463734
8	0.4872159703358772	0.5030102716478038
9	0.508774199111801	0.5909257837278403
>10	9.076014314663908	26.85373752211799
>50	1.5608157633768809	13.895102326978934
>100	1.5349458888457723	38.11193091398747
>500	0.038804811796662786	3.0942921688422973
>1k	0.012934937265554261	2.695890228023904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1974	1.0983874737088106	No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1809	1.0065769705872534	No Hit
TCAAAGGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG	1062	0.5909257837278403	No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	719	0.4000712226933306	No Hit
TCAAAGGGGACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT	668	0.3716934308193948	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	656	0.36501630331964524	No Hit
TCAAAGTGGCTAATTCTCTTTCCTGTACTTTAATAGACTGACAGCCTCATGG	641	0.3566698939449582	No Hit
TCAAAGGGGACAAGCCCTTTGGGGGGATGCTGCAGAAATCTGGCACTCCCCC	619	0.3444284935287506	No Hit
TCAAAGGGGGTAAGGAAGTCACTGATGTGTGTATTTTTGTACTGAGAGTAAT	580	0.3227278291545644	No Hit
TCAAAGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA	569	0.3166071289464606	No Hit
TCAAAGGGGAGCTTTGTTGCAGGTGTTTCTACCAGTGCTGCGGATTTGGGTG	566	0.31493784707152317	No Hit
TCAAAGGGGAACTCAGGAATTTGTAGAGGCAGAAGCAATAGAAGATAGAGAA	543	0.30214001936366974	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	492	0.2737622274897339	No Hit
TCAAAGGGGTCATCTCCTCATTACAATTAAATTAGCTTTGCCAGATAAGAAG	487	0.2709800910315049	No Hit
TCAAAGGGGGGATGGAGCCACCCTTCCCCCTGCCCTGAACAGCCAGGGTGGA	466	0.2592951179069431	No Hit
TCAAAGGGGAGTGCAGTGCTTCAAAGGGGACCTGGAGGTCTTTGCCCCTAAT	453	0.25206156311554767	No Hit
TCAAAGGGGGAGGGGGGAGGGGCAGGGCCGCAGGGTGTCATGGGAGAGGGGA	413	0.22980447144971566	No Hit
TCAAAGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGG	379	0.21088594353375845	No Hit
TCAAAGGGGGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA	375	0.20866023436717523	No Hit
TCAAAGGGGTGCCTACTGTATTGCTTTGTGCTCTCACCTCTGATTTCACTGG	371	0.20643452520059205	No Hit
TCAAAGGGGACTAATCGGACTGCCAAATTTCACCGGTTTGCCCTGGGATATT	355	0.19753168853425923	No Hit
TCAAAGGGGAGCTGCCAAAGCTTAGGCACTCTCTACACTCTTGTGTAAAAGT	353	0.19641883395096765	No Hit
TCAAAGGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	340	0.18918527915957223	No Hit
TCAAAGGGGGGTGGGGCCTGGACCTACAACACCACCTCTGCAGTCACAGTGA	339	0.1886288518679264	No Hit
TCAAAGGGGCTGATGCCCTGTGTGTGTGTGTGTGTGTGTATTTGGGGCTGCA	335	0.18640314270134323	No Hit
TCAAAGGGGCCTGTCCAAATTCTTCCTACTGGTCAATGCCCATCCCAAGTTC	332	0.1847338608264058	No Hit
TCAAAGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA	331	0.18417743353476002	No Hit
TCAAAGGGGCGTGGGCCCCGTCTCCACCCCTGGCCCTGCCCAGCCTGTGTAG	330	0.1836210062431142	No Hit
TCAAAGGGGATTGGTAAAAGACTGTATAGCATCTATTTATTTAGATGATTTA	329	0.1830645789514684	No Hit
TCAAAGGGGAAGGCAACTGTGGCCAATATTTATATCATGACATACAAATGGA	326	0.18139529707653101	No Hit
TCAAAGGGGCTCCACCATCCGAAGAGATGCCCCTGCTGGGCGCAAAGTGGGT	322	0.1791695879099478	No Hit
TCAAAGGGGATTGGGAACCCATTTTCCACCTGGTCTTTCTTGACAGGGTTTT	322	0.1791695879099478	No Hit
TCAAAGGGATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGG	319	0.1775003060350104	No Hit
TCAAAGGGGCGTACACCTGTAATCTCAGCCACTCAGGAGGCTGAGGCAGGAG	317	0.1763874514517188	No Hit
TCAAAGGGGACGTACCCCGTACCCATAGCTTAGTTTTCTGGGAGCAGAATGG	316	0.175831024160073	No Hit
TCAAAGGGGAGCGACTGGATGCTCGCCATCAAAGTCCAGTGGAAATTCTTCA	308	0.17137960582690662	No Hit
TCAAAGGGGGGAATTTCGATTCTGTGGATAGGGAAGTGGGATTGGTATCTGT	306	0.17026675124361498	No Hit
TCAAAGGGGCAAGGGAATCAAGAGCCCTGGGCTGAGCTGTGCTCTGCCAGTG	303	0.1685974693686776	No Hit
TCAAAGGGGAGCTGGAGCTGCGCCTGCTGGTGGTGCTGCTCCATCCACTGCC	303	0.1685974693686776	No Hit
TCAAAGGGGACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCTGT	300	0.1669281874937402	No Hit
TCAAAGGGGAGTTGTGCCAAATTTGGGTCAGAGCGCAAATCAGAAGGTTATT	297	0.1652589056188028	No Hit
TCAAAGGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGG	293	0.1630331964522196	No Hit
TCAAAGGGGAAGGACAAAGTGGTAAAAAAGGAAATAAAGACAAAACTCGAGG	293	0.1630331964522196	No Hit
TCAAAGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	291	0.16192034186892798	No Hit
TCAAAGGGGACTGTGGGGATAACATACACTGTAATGGGGGAAAATTACCTAA	291	0.16192034186892798	No Hit
TCAAAGGGGACCTGGGGAAGCTTTGCATACCCAGCCTAGACCGATCAGATTT	291	0.16192034186892798	No Hit
TCAAAGGGAGGCTTTTTTTAAAAAAAAAGTGTTTCTGTGCCATTGTTCGTAT	289	0.1608074872856364	No Hit
TCAAAGGGGTACAGATTCTGGGGCCTCGGGGCCTATGTTCTGAACCGTTAGG	287	0.1596946327023448	No Hit
TCAAAGGGGATTCTAACCACCTACAGCTGGCCCCGAGCCAAGCCTTCCAGGC	285	0.15858177811905316	No Hit
TCAAAGGGGGGACAGTAGACTGTTCTGTAATTAAAATGTAGTATACTGCTTT	281	0.15635606895246998	No Hit
TCAAAGGGGGATCATTGTTTGTGATTGCATGTTTCCTTCAACTGTGTTCTCC	280	0.1557996416608242	No Hit
TCAAAGGGGACGGTCTCTTTGGTTTCTGTCTGTAAGTTACGGCCCTGGATGT	279	0.15524321436917837	No Hit
TCAAAGGGGATGAATACTTACGTTTACCTCACTGGTGCATTTAAAATGGACT	279	0.15524321436917837	No Hit
TCAAAGGGGCAGAGTGCTGTGCTTCAAAGGGGATCCCACAGTTGTAGTTTGT	277	0.1541303597858868	No Hit
TCAAAGGGGAACCTTGCGTGCCATTCGGTCCCATTCCCATCTGCTCACTACA	277	0.1541303597858868	No Hit
TCAAAGGGGAGTTGTGGGTAGGAAGAAAGTTAATGTAGCCCTGTGGTTTTTC	276	0.15357393249424098	No Hit
TCAAAGGGGATAGTCATGATGGTCCGGATGCGGGTACCGTGCTTCACGGACT	274	0.15246107791094937	No Hit
TCAAAGGGGGCGAGGCGGAGTCCCGCTCCCGTGGGGAGGGGTGTGTACATAA	274	0.15246107791094937	No Hit
TCAAAGGGGCAGAGTGCAGTGCTTCAAAGGGGGACGATAACTGATAGTGCAT	272	0.15134822332765777	No Hit
TCAAAGGGGAGACAGAGTACTTGGAGGAGAGGAGGGTCAAGGAAGTGGTGAA	272	0.15134822332765777	No Hit
TCAAAGGGGGCCGAACCCACGTCGGTAGAATACGAGCGACCGGCAGGGCTCA	272	0.15134822332765777	No Hit
TCAAAGGGGCTCTGGGAACCCAGGGTCCCCAGATTTTCTGAGACGGAAGTTC	269	0.14967894145272037	No Hit
TCAAAGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG	268	0.14912251416107458	No Hit
TCAAAGGGGAGCGGGCAAGGTTTATACGTGCCTTTTTGTTTTTGTAAAAAAG	266	0.14800965957778295	No Hit
TCAAAGGGGGACGCACGCCTTTAATCAGAGCACTCGGGAGGCAGAGGCAGCG	265	0.14745323228613716	No Hit
TCAAAGGGCTTCGCTCGCTCAACCTCTTTTGTTCAGTACGTGTAACTTGAAG	264	0.14689680499449137	No Hit
TCAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	264	0.14689680499449137	No Hit
TCAAAGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	263	0.14634037770284558	No Hit
TCAAAGGGGATTCTGGGACAGTGCTGGGAGCATGGTAGGAGTGTGCAGCAGG	263	0.14634037770284558	No Hit
TCAAAGGGGCCCCACAGCATGAGGGATCTACCTAGACCAGGGATCGAACCCC	263	0.14634037770284558	No Hit
TCAAAGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	262	0.14578395041119976	No Hit
TCAAAGGGGAAGCGAGTGAGTGAGTTGAGTTACCTGAACTCTGTAGCACTGG	262	0.14578395041119976	No Hit
TCAAAGGGGCTTAGGAAATGTGCAAATGAGATCCAACTCCAATTCAACAATC	262	0.14578395041119976	No Hit
TCAAAGGGGAGTTGGAGAGTGCAGGCCCCTGAGCAAGACTGTGCGATTCAAT	261	0.14522752311955398	No Hit
TCAAAGGGGATCTTCCCTCCCCTTTGTCTCTGAGAGAGTGCTTCGCGCACGT	261	0.14522752311955398	No Hit
TCAAAGGGGGACCCGTCAATCATGGGCGGGATGATTGTCCGAATTGGGGAAA	260	0.14467109582790816	No Hit
TCAAAGGGGGCTTTCACATCCTTTGCACCCTACCCCCATCATGATGTTGTCA	260	0.14467109582790816	No Hit
TCAAAGGGGTACAGGGTTGTTGATTTGTTCACAAAGGAAACTGCCCAGAAAA	257	0.14300181395297076	No Hit
TCAAAGGGGAGGGATTCATAGAGTTCCGGGCCTGGGATTCTCCCAGCTGCGC	257	0.14300181395297076	No Hit
TCAAAGGGGGAGAAACATTCATAGCTGCAAAAACCATCATAAGTAGAGGACT	257	0.14300181395297076	No Hit
TCAAAGGGGACCCACCAAGGAGACAGACATAGCCAGACTCAACTCTGGAAGG	256	0.14244538666132497	No Hit
TCAAAGGGATGAAGGCTCTTGGCATTCCTTTGTAGATAGGAAGTTCCTTGTC	255	0.14188895936967916	No Hit
TCAAAGGGGAGCGTTTGGATGGATGAGGTGTAGGAAAGCTGTGGAGCAGCTG	252	0.14021967749474176	No Hit
TCAAAGGGGGTGCTGACTTTCCATGTGACCATTAGACTTTAAGGGATATAAG	251	0.13966325020309597	No Hit
TCAAAGGGGAGGGTTTTGGTTTTTGTTTTGTTTTGGTTTTGTACTAAGAATA	251	0.13966325020309597	No Hit
TCAAAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	248	0.13799396832815855	No Hit
TCAAAGGGGCACTGGGGCGCAGCAGCGCGCTGCCCCTCTGCCCCAGGGACAC	247	0.13743754103651276	No Hit
TCAAAGGGGGTGGGTGGTTGGATAACTCTAGATGACTCGGGGTGCATACCAC	247	0.13743754103651276	No Hit
TCAAAGGGGGACGGCTCCAGTGGAGGGGTGATCCGCTTGGCAGCCATTCAGG	245	0.13632468645322116	No Hit
TCAAAGGGGATTTGAACATCATTTTAATTATTACAGGACAATGATAAGTGCA	245	0.13632468645322116	No Hit
TCAAAGGGGAGTTGGATCTTGGGAGCGTCCCCGCCCCTTGCCTCTCGGCGCC	245	0.13632468645322116	No Hit
TCAAAGGGGCACCGTCCGGGCAGACCATGGTGAGCCCATTGGCCGGGGTACC	244	0.13576825916157537	No Hit
TCAAAGGGGCTCTGACAGTCTGGTCACCGGTCCTGGCCCACCCTCCCCTTCT	243	0.13521183186992955	No Hit
TCAAAGGGGGCATAGGTGTGACCCATAGCTTTCTTGTCCTTGTAAGAGGTGG	243	0.13521183186992955	No Hit
TCAAAGGGGAGTTTGTGTCCCTGGGCCTGCCCCCATCGGCCCTGCTGATGGG	243	0.13521183186992955	No Hit
TCAAAGGGGCAGCCTCCTGAGAGTCTCAAGCTCGAAGCTGGAGACAAGAAGC	241	0.13409897728663794	No Hit
TCAAAGGGGACTCAAGAATGACCCCATGGTCTGTGGGGTGGGCCAGAGTGTG	239	0.13298612270334637	No Hit
TCAAAGGGGATGGTCCCCTATTCAGACACTAGGCCAGGTGTGGCTGGGGCTC	238	0.13242969541170055	No Hit
TCAAAGGGGATGAGTGACAGGACGGTGCTGGTTTTCTACCTGCTTACTCTTG	238	0.13242969541170055	No Hit
TCAAAGGGGGTGCAGTTCTTCAAAGGGGTGTTATTAATACTGAACTCCATTC	237	0.13187326812005476	No Hit
TCAAAGGGGAATTCTTTACTGTGGAATAGCTTGGGACATCAGCTCTGTTTTA	236	0.13131684082840894	No Hit
TCAAAGGGGATTTGCACTTTGAAGAATGTAGAGAGTAAAAAAACTAAAATTA	235	0.13076041353676315	No Hit
TCAAAGGGGGTGAGGGAGGTGGGGAGGGCATGGTTTATGTTGAATGTTTAAA	234	0.13020398624511734	No Hit
TCAAAGGGGGAAGAAGCATTCTCAGTTCATTGGCTATCCCATTACTCTCTTT	233	0.12964755895347155	No Hit
TCAAAGGGGATTCGGTCTGCATTCCCAGTGGGCAGTGATGGCATTACTCTGC	232	0.12909113166182576	No Hit
TCAAAGGGGGAGGCAAAGTTGGGCCAGCTGTTTCTGGGTGTGCAGTTAGAGT	231	0.12853470437017994	No Hit
TCAAAGGGGGTCTTACAACTGAACATGTATGTGTGTTGCTTAGATAAATGTA	231	0.12853470437017994	No Hit
TCAAAGGGGAGGGCCACTCTAGAAAGGCCAACAGCCTTCCGGTGGCTTCTCC	230	0.12797827707853415	No Hit
TCAAAGGGGGTCGAGCCCAGTGTTACCACCAAGAAGGTCAAACAGGAAGACA	230	0.12797827707853415	No Hit
TCAAAGGGGGAAGACTTATACCATTGTGTTTAAGCTGTATTGAATTATCTGT	229	0.12742184978688834	No Hit
TCAAAGGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	228	0.12686542249524255	No Hit
TCAAAGGGGATGTAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATT	228	0.12686542249524255	No Hit
TCAAAGGGGGAAAAGCCTGGAGGAGAAAACTTGGAATTTTTGTAATTCTTTA	227	0.12630899520359673	No Hit
TCAAAGGGGGTTTTGTATCAGTTCTTTTCTTTGTAAATGGCATTAACATTGT	226	0.12575256791195094	No Hit
TCAAAGGGGAGTCTAAGTACCAATGCTATTGTAAACAACGTGTATAGTGCCT	223	0.12408328603701355	No Hit
TCAAAGGGGACAGCTATGGGACAGCAGCCATGGGCTGTGTCTACATGGGCAC	222	0.12352685874536774	No Hit
TCAAAGGGGATTCTGTTTGGTGGCTGATAAAGAGACTTTGAGATTACAAAAT	222	0.12352685874536774	No Hit
TCAAAGGGGGTCTCCCAGCCTTGGGGCCCCCGGAGCCTCCCCTCTTCTCCAA	222	0.12352685874536774	No Hit
TCAAAGGGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG	222	0.12352685874536774	No Hit
TCAAAGGGGGTGCTTCAAAGGGGAGCCATACTCTGCTATGTAATGTTATAGT	221	0.12297043145372195	No Hit
TCAAAGGGGGTTCCTGCCCTTGTGCCTTAGTTCAGTTCCTACCAGAGAAGTG	220	0.12241400416207614	No Hit
TCAAAGGGGGATAGCTTTACCTGGCCCAGCATCAAAGCTGTTGACAAGCAAG	220	0.12241400416207614	No Hit
TCAAAGGGGAGCTGGATGGTGTGGATGATGGGACAGAGAGTGACACCAGTGT	218	0.12130114957878455	No Hit
TCAAAGGGGTGTAGGCCCCACCACATCCACCACATGGTCCCCAGCCAGTATG	217	0.12074472228713873	No Hit
TCAAAGGGGACTTGTCTGAGGGTAAAGGGACCGCTTTGTCACAAATGCTATA	217	0.12074472228713873	No Hit
TCAAAGGGGCTACTGGGTTGGTGAAGATTCCACATATAAATTTTTTGAGGTT	216	0.12018829499549294	No Hit
TCAAAGGGATGTAGAATCACCTTTGATTTCATGGCAATTGACAGTCCTAATG	216	0.12018829499549294	No Hit
TCAAAGGGGAGGGAACAAAGCATCATAAATAAATCAGGAGTGAAAAAGAGGT	214	0.11907544041220135	No Hit
TCAAAGGGGAAATGGGACTGATGTCATCTTGAGCTTTTATTTTGACCGTGAT	213	0.11851901312055553	No Hit
TCAAAGGGGAATGAAGCTCTGAAATAAAATGGGACCGTTGAGATGACTTCCG	212	0.11796258582890974	No Hit
TCAAAGGGGAGCTAAAACATTGTTAAAAAAAAATAAAATTGACTTTGAGTTC	212	0.11796258582890974	No Hit
TCAAAGGGGATATCCCGGGGAGAAGATGGGCTGTCCCCACCCACGGGGCCGT	211	0.11740615853726394	No Hit
TCAAAGGGGGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCC	210	0.11684973124561812	No Hit
TCAAAGGGGAATCATAACAGACAACACAGAAATACAAAGGATCATAAAACAT	209	0.11629330395397233	No Hit
TCAAAGGGGAGCCACAAGACAGCCTTTACTGAAAAAGCTATTGGACTCTTGC	209	0.11629330395397233	No Hit
TCAAAGGGGATATCCTCACCGCCCCAGCCTGGTCCCAGGCATGGCTGACTCT	209	0.11629330395397233	No Hit
TCAAAGGGGATGTTTTTTGTGTGTCAATTGTGCCATTTTTATTTTATTCCTT	208	0.11573687666232653	No Hit
TCAAAGGGGTATATCTACAGCTAAGTATCACGTTACTACATAGCACCACAAC	207	0.11518044937068074	No Hit
TCAAAGGGGGGAAGTACCAGGCAGTGACAGCCACTCTGGAGGAGAAACGGAA	207	0.11518044937068074	No Hit
TCAAAGGGGGGTGAGGGAAGAAAACACACTCAACACCAAAGGGTCATCTTTC	206	0.11462402207903494	No Hit
TCAAAGGGGACTGTTTTCTCTGTCTGCTGTCTAACCCTGTGCCTTGCCTGGG	205	0.11406759478738915	No Hit
TCAAAGGGGGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTTGTCCCCCCA	204	0.11351116749574333	No Hit
TCAAAGGGGGTCTTGTATTTGTTTTGTTCTGTTTTCTCATTGGGGGTAAATG	204	0.11351116749574333	No Hit
TCAAAGGGAATTAAAAAAAAGCATATTTGTCACGGAAATTTAGTTACCGTCT	204	0.11351116749574333	No Hit
TCAAAGGGGATTCATTCTTTAATTTATGTAAGGTTTTTTTGTACTCAATTCT	203	0.11295474020409753	No Hit
TCAAAGGGGGACAGTTGTGTGACTTTGTGTGGTCATGTTATGGATCTTACCT	203	0.11295474020409753	No Hit
TCAAAGGGGAATGAAGGGGGGTGACAAGGTCTCCCAGGGGAGTTGGGAGGAA	203	0.11295474020409753	No Hit
TCAAAGGGGCAGGAGCTCCTTAGTAAAGGACTTATCAAGCTGGTTTCAAAGC	202	0.11239831291245174	No Hit
TCAAAGGGGAGTGCAGTGCTTCAAAGGGGAGCCATGCTCGATGCGCTTCAAA	202	0.11239831291245174	No Hit
TCAAAGGGGGATCCACTAAGGAAATGTTTTATCAGCTAGAGACCACTGCTTG	200	0.11128545832916013	No Hit
TCAAAGGGGGTTTTTAAAATATGAATGCATACTTGTAATATATTTTTTAAAA	200	0.11128545832916013	No Hit
TCAAAGGGGAGGAACCAAGGACTGCCAGAAACTCTCTGCCTCTACGTTATGC	198	0.11017260374586854	No Hit
TCAAAGGGGAAATTTTGGAGTACATTTTTTCTGTTTTCTTAATTAGACCACA	198	0.11017260374586854	No Hit
TCAAAGGGGAGCGGTATGGTTCGTGCCAAATTCCGAAGCAACCTTCCTGCAA	198	0.11017260374586854	No Hit
TCAAAGGGGGTTCCTAACTCCTTTTTGTTATACACCCCATCCATGATCATGT	197	0.10961617645422272	No Hit
TCAAAGGGGGTACATAAGTAACACGAGATGCTCTGTATGTGTGAGTGCGTGT	197	0.10961617645422272	No Hit
TCAAAGGGGTAATACAGAGATAAAGCAAGAAACAAAAGGGAAAAAATGCAAA	197	0.10961617645422272	No Hit
TCAAAGGGGATCCAATGTATGTCAGCCATTGTCTCTGCTGGCTTGTGACTTT	196	0.10905974916257692	No Hit
TCAAAGGGGAGAATATACATACCTGTAGGATGCTGTGTAATGGGAAACCATA	196	0.10905974916257692	No Hit
TCAAAGGGGGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG	195	0.10850332187093113	No Hit
TCAAAGGGTGACCCATTACAAGCACGGAAATTGAAACTGTAATCAAAAATCT	195	0.10850332187093113	No Hit
TCAAAGGGAGTGCAGTGCTTCAAGGGGTACCCTGCGGTTCCTGCCTCAACTG	195	0.10850332187093113	No Hit
TCAAAGGGGATTGTAGCTACCACATTGGTTTTTCTATAATCACGCGGGGTTT	195	0.10850332187093113	No Hit
TCAAAGGGGCATGCCTGCACCTGGGTCGGGGTGCTCTGGGACTGGGAGATAG	194	0.10794689457928532	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTGAGAGCACCCGACTGCT	193	0.10739046728763953	No Hit
TCAAAGGGGATGGGGAACTTCTTCGGATGGGAAATGGAATCGTGAATTCACC	193	0.10739046728763953	No Hit
TCAAAGAGGGAAGCTGAAGATGACAACCTTGATGACCTAGAGAGGCACCTGC	193	0.10739046728763953	No Hit
TCAAAGGGGAAGCCAGACATACAAGGTGCTCAGACATGTGCTGTACATGGTT	193	0.10739046728763953	No Hit
TCAAAGGGGCAGAGTGCAGTGCTTCAAAGGGGACGCTCCAAGGATGTATAGC	193	0.10739046728763953	No Hit
TCAAAGGGGAGCTAATCAGATCTGCCTCAGGAAAAATACTAACTTGTTCTAT	191	0.1062776127043479	No Hit
TCAAAGGGGACTTTTTTTCTTAAAAATACTTTAAAAGGAAAATTTGTTTGTA	190	0.10572118541270212	No Hit
TCAAAGGGGGCTGTGGGAGCTTGGATCAATGCTGAAGTCATTTTCATTGTAG	189	0.10516475812105631	No Hit
TCAAAGGGGATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	188	0.10460833082941053	No Hit
TCAAAGGGGAAGCCTTGATGAGTGACGTTAGCTATATGGATGTGTAATCCTC	187	0.10405190353776472	No Hit
TCAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	187	0.10405190353776472	Illumina Single End Adapter 2 (97% over 34bp)
TCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAACGAACCA	187	0.10405190353776472	No Hit
TCAAAGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	187	0.10405190353776472	No Hit
TCAAAGGGGTGCAGTGCTTCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGT	186	0.10349547624611893	No Hit
TCAAAGGGGAAGATTCAGAAGCTCAGCAAGGCCATGCGGGAGATGCTCTGAG	186	0.10349547624611893	No Hit
TCAAAGGGGGGGGTAAGGGATATGGCTAAACTAGGAATCTGGAATTAAAATA	186	0.10349547624611893	No Hit
TCAAAGGGGATGTGGGTTGTGGGTGCTGTCCTGAGGATTCAGTAGCCGATGT	186	0.10349547624611893	No Hit
TCAAAGGGGTGGGCAACATAGCAAACCTGGTATCTACCCCCCCCACACACAC	185	0.10293904895447312	No Hit
TCAAAGGGGAGCCTTCACAATTTTTTAATAATCAGTATCATTTCTACACCAA	185	0.10293904895447312	No Hit
TCAAAGGGGCTCTATTTTGCCACAAGCGTCTCGGGATTGTGTTTGACTCCTG	184	0.10238262166282731	No Hit
TCAAAGGGGATGCCACAGAGCTGTCAGAATGCAGCACAGCTCCAGCAGCCAC	183	0.10182619437118152	No Hit
TCAAAGGGGGATGTTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGG	183	0.10182619437118152	No Hit
TCAAAGGGGAGTTTTCCCTGAGTGAGGCTGAAGCCTCAGGTTCCTTCAAAAC	182	0.10126976707953571	No Hit
TCAAAGGGGGCATGAAGGCCGATGGTAACCTGGTGTACATTAAAATAAGTGT	182	0.10126976707953571	No Hit
TCAAAGGGGAGGACTCTCAACTTCCAATGACTGTTGTGATGGCCTCCCCACA	182	0.10126976707953571	No Hit
TCAAAGGGGAGCGCGGCGGCGGAAACCGCAGCCCGGCGGCCCTGGAACCTCC	182	0.10126976707953571	No Hit
TCAAAGGGGGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGT	180	0.10015691249624412	No Hit
TCAAAGGGGAGGCCCTTCCAAGGGAAATCTCTGCCCTCTGCATCCTCAGCTT	180	0.10015691249624412	No Hit
TCAAAGGGAAGAGGTGTTATTTGAGCCTTGTTTTGGACAGTATACCAGTTGC	180	0.10015691249624412	No Hit
TCAAAGGGGAACGAGGGCCGCGAAAGCGCGGTTATGAATATTAATGAGGCCG	180	0.10015691249624412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.09793120332966092	0.0	0.0	0.0	0.0
6	0.09793120332966092	0.0	0.0	0.0	0.0
7	0.10015691249624412	0.0	0.0	0.0	0.0
8	0.10238262166282731	0.0	0.0	0.0	0.0
9	0.10961617645422272	0.0	0.0	0.0	0.0
10	0.15246107791094937	0.0	0.0	0.0	0.0
11	0.19697526124261341	0.0	0.0	0.0	0.0
12	0.22368377124161185	0.0	0.0	0.0	0.0
13	0.24371515374086067	0.0	0.0	0.0	0.0
14	0.2670850999899843	0.0	0.0	0.0	0.0
15	0.28989861894746216	0.0	0.0	0.0	0.0
16	0.31827641082139796	0.0	0.0	0.0	0.0
17	0.3399770751955842	0.0	0.0	0.0	0.0
18	0.36223416686141624	0.0	0.0	0.0	0.0
19	0.37892698561079025	0.0	0.0	0.0	0.0
20	0.4050790683181429	0.0	0.0	0.0	0.0
21	0.4262233054006833	0.0	0.0	0.0	0.0
22	0.4445854060249947	0.0	0.0	0.0	0.0
23	0.47073748873234733	0.0	0.0	0.0	0.0
24	0.49800242602299155	0.0	0.0	0.0	0.0
25	0.5297187816468022	0.0	0.0	0.0	0.0
26	0.5597658553956755	0.0	0.0	0.0	0.0
27	0.5870307926863196	0.0	0.0	0.0	0.0
28	0.6309885487263379	0.0	0.0	0.0	0.0
29	0.6582534860169822	0.0	0.0	0.0	0.0
30	0.6844055687243348	0.0	0.0	0.0	0.0
31	0.711670506014979	0.0	0.0	0.0	0.0
32	0.7311454612225821	0.0	0.0	0.0	0.0
33	0.7539589801800599	0.0	0.0	0.0	0.0
34	0.7751032172626003	0.0	0.0	0.0	0.0
35	0.8023681545532445	0.0	0.0	0.0	0.0
36	0.8212866824692018	0.0	0.0	0.0	0.0
37	0.8396487830935132	0.0	0.0	0.0	0.0
38	0.8563416018428872	0.0	0.0	0.0	0.0
39	0.8691394295507406	0.0	0.0	0.0	0.0
40	0.8836065391335314	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAC	25	3.4055796E-5	46.0	37
TGATCCG	20	6.29718E-4	46.0	29
TCACGCG	25	3.4055796E-5	46.0	40
TCCGGAA	25	3.4055796E-5	46.0	36
GCCCCTC	20	6.29718E-4	46.0	32
CTTCGTG	25	3.4055796E-5	46.0	41
TTTAGTC	20	6.29718E-4	46.0	35
CAGGTTC	30	1.852588E-6	46.0	37
GCCCCGT	55	1.8189894E-12	46.0	15
ACAACGT	30	1.852588E-6	46.0	35
GTGTTCC	20	6.29718E-4	46.0	21
GTGTTAC	20	6.29718E-4	46.0	20
CTTCGGA	40	5.5661076E-9	46.0	21
AGCGTTT	20	6.29718E-4	46.0	10
CTTCGCG	40	5.5661076E-9	46.0	41
CTTCGCA	20	6.29718E-4	46.0	38
CTATGTT	25	3.4055796E-5	46.0	33
CAGGTGC	20	6.29718E-4	46.0	18
CAGGTCT	30	1.852588E-6	46.0	18
AACGTGC	20	6.29718E-4	46.0	24
>>END_MODULE