FastQCFastQC Report
Fri 17 Jun 2016
SRR1527459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17255
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7184.16111272095045No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3852.231237322515213No Hit
AACGCCGGGACCCCAATCCCTGATTCCCTCAGCTCCGCAGCGAGAGCGGT3802.2022602144306No Hit
AACGCCGGGAGACCTGGCATCACCCAACCACCAGTAGCATCCTGTGTGGG3431.9878296146044625No Hit
AACGCCGGTAGTAGAGTTGGAGACAAGGTCCTACCTCCTCTGTGGGCTGC3401.9704433497536946No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2971.7212402202260213No Hit
AACGCCGGGAACACCATACACAAAAATAAACTCAAAATGGATTAAAGAGC2851.65169516082295No Hit
AACGCCGGGCATCCGGAACTTGTTTTAGCAGAGCGCTTGCCTGGTATAAA2831.6401043175891046No Hit
AACGCCGGGGGGGGCCGCCCTCTCGCCCGTCACGTTGAACGCACGTTCGT2621.5184004636337294No Hit
AACGCCGGAAAGCAAGAAAGATGAGAGACAAAAGATAGACAGTGAGTATT2451.4198782961460445No Hit
AACGCCGGGATCTTGGTGTGGTATTGCCTACCTGCAATCCCAGAACTCAG2411.396696609678354No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGCCGGGA2271.3155607070414372No Hit
AACGCCGGCTGTGATGTGTTTAATTTGAGGGCATCTCCTGACTTCTAGCT2011.1648797450014488No Hit
AACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGT1951.1301072152999132No Hit
AACGCCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1921.112720950449145No Hit
AACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGC1921.112720950449145No Hit
AACGCCGGGGGAGTCCAACTCTCACATCCATACATGACTACTGGAAAAAC1881.0895392639814547No Hit
AACGCCGGGGGATTTAGGGTGTTAGGTTGTTTTGTTTAATTATTCCATAT1841.0663575775137641No Hit
AACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACGATAAA1610.9330628803245435No Hit
AACGCCGGGAAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAAGT1550.8982903506230079No Hit
AACGCCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.8924949290060852No Hit
AACGCCGGGTGTGCGCCATAACTTCTGAAGCCCAGGTGCCTACAGCCTGT1460.8461315560707042No Hit
AACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAA1440.8345407128368589No Hit
AACGCCGGGGCATCAAGACTGACTGAAAAAATGCCGTGCGAAAAACATGA1410.8171544479860909No Hit
AACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAA1390.8055636047522458No Hit
AACGCCGGGGCGCCGAGAGCCGCGGGGAGAAGGCCATCACCCAGGTCAGC1300.7534048101999421No Hit
AACGCCGGGCCTTCGTGTTGACTTCATTCACAGGTTGGAGTTCGGAGAGG1290.7476093885830194No Hit
AACGCCGGGAACCTCTGTACTTGCAGACCATAGCAAATGTAGCTTTTAAA1280.7418139669660968No Hit
AACGCCGGGCCACGTGATCAGTGCGTGCACAGTAACATGATCAGTGCCTG1270.7360185453491741No Hit
AACGCCGGGATGACTACTTGTACTCTGCTTTTCCCGTGAACGTCAGCTCG1240.7186322804984062No Hit
AACGCCGGGATGTCCTTGTAGGCTCTGTTTTAAGAAAACCATGTGGGAAA1080.6259055346276442No Hit
AACGCCGGGAGGCATTCATCTCGAGTGGAAGCAAAGAACCTCGCTCTGCT1050.6085192697768762No Hit
AACGCCGGGACTATTACAAAAAAAGAAAATGCTTAGAAAAGATTTAAGCA980.5679513184584178No Hit
AACGCCGGGGGCTCTTTTGCCTGCCACCAAAGACATGACTTTGCTCCTCA970.5621558968414953No Hit
AACGCCGGGAATCAAAAAACCAAACAAACAAACAAACAAAAAATACCCAT950.55056505360765No Hit
AACGCCGGGGGTGCTTAGGTGCCCGAGCTACTGAGGGTCTAAGTCCGGGC940.5447696319907274No Hit
AACGCCGGGCTCTACTTTGTGCTGAGTGGTTGCAATGGCGGACGTGTCTG860.4984062590553463No Hit
AACGCCGGGGAACCCGGGTCTCCTACACTGCAGGCAGATTCTTTACTGTC830.48101999420457836No Hit
AACGCCGGGGATCCATGCCCTTGGTGGCACCGTTCTCCTAGCATCTCAGA770.4462474645030426No Hit
AACGCCGGGAGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAG710.4114749348015068No Hit
AACGCCGGGATCACCGTGTCTCAGGATGAGGGTGTCCGTCCCAGCACCAC700.4056795131845842No Hit
AACGCCGGGCAGATGGTACTCTGTATGTCATGGACATGTTTGTACCTGTA680.3940886699507389No Hit
AACGCCGGGAGTACCCACCTGGTCGTGGGGGTCCTCCTCCACCTATGGGC670.3882932483338163No Hit
AACGCCGGGAACTATTTGTAACTAGTTTCAGGGCATCTAACACCTCTTCT670.3882932483338163No Hit
AACGCCGGGGGACCGTCCTGGCTGCCAGTTACACTGCGATTGTAACAACA630.36511156186612576No Hit
AACGCCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGC620.3593161402492031No Hit
AACGCCGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.3593161402492031No Hit
AACGCCGGGGAGCGATGCCACTGGACAGAAAAAAATTTACAATAATCCAG620.3593161402492031No Hit
AACGCCGGGATCTCTGTGGATCTTAGCCTCCCTTGAAGGGCAAGCCTGCA610.3535207186322805No Hit
AACGCCGGGGTGCCGCTGGCCCGTCAGGCCCCAGCTGCCTCCTGTGCATG610.3535207186322805No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.3535207186322805No Hit
AACGCCGGGGCTGGTTCTCCAGCACTTCTGCACCTCGGGAGCCTGCCCAA600.34772529701535787No Hit
AACGCCGGGATGGAGAGGGAGCTGGAAGGCCAAGGTGGGGCTAAGGAACC600.34772529701535787No Hit
AACGCCGGGCACAGTGGAGAGCCCGGCAGAGCAAAGCCTGAGACTGAACA600.34772529701535787No Hit
AACGCCGGGTGCAGTGCTAACGCCGGGGGGTAGTGGTTTTTAAAGAACTT600.34772529701535787No Hit
AACGCCGGGGATATTATATTTTACAACCTCCAATGAAATAATGTATGCTG570.33033903216459No Hit
AACGCCGGGATTCCTCTTCTTCCTCTTCTTCCTCTTCTTCCCCTTCTTCT570.33033903216459No Hit
AACGCCGGGCAGTGCTAACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAA560.32454361054766734No Hit
AACGCCGGGAACCATGAAGAGAGAAACTGTCATTATATTACAAGAAGAAA550.3187481889307447No Hit
AACGCCGGGAGATAAGGAGCAACGGCTATGCTTTGCTGGAGCAGCCGTGA540.3129527673138221No Hit
AACGCCGGGACAGCGTTTTCGTGTCCTGAAATCGCTTTATGCTGCAAAAG540.3129527673138221No Hit
AACGCCGGGATGCATAGTGCTCCTACACTTAGGATGAGAATGATCTTCTT530.30715734569689945No Hit
AACGCCGGGAGCAAAATTACAGTCATGAAGTAGCAATGAAAATAATTTTA530.30715734569689945No Hit
AACGCCGGGAGGCTAACCTCAGAAGCATCAAATTCTGGTACACAAAACAG510.2955665024630542No Hit
AACGCCGGGGTGAGGTGTTTGCATGTGGCCATTCCCGTCACTGGCCTGTG490.2839756592292089No Hit
AACGCCGGGGGCCGGGTGGCGACTGGCTCCCGGGTCTGAGGGGCTTCTGC490.2839756592292089No Hit
AACGCCGGGGAAGCCACTGTTGTTGGCTGTGTGACAGTGCGTGTGTTATT480.2781802376122863No Hit
AACGCCGGGACGATGTGAAAGCTCCAGCCATGTTCAATATTAGGAACATC470.2723848159953637No Hit
AACGCCGGGGGGGAAACCCGTAGCCCTTGCAGCATGGCTGACCAACTGAC450.2607939727615184No Hit
AACGCCGGGTTTTCTCTCTCTTGCTTCTTGCTCTCTTTTCCTGAAGATGT450.2607939727615184No Hit
AACGCCGGGATGGTCAAGTTTAAGGAGAATCAGATCTGTTGAGGTAACCT430.24920312952767315No Hit
AACGCCGGGATGGATGGTAGTAATAAAGATTGTTTATCATTCACAAAGCT420.2434077079107505No Hit
AACGCCGGGAGACCATAGCAAAAATCAACAAAGCCAAAAGCTGGTTCTTT410.23761228629382786No Hit
AACGCCGGGTGGGATGAGCCCTGCACGGACGGCATTGGGTGGAAAAGGCC410.23761228629382786No Hit
AACGCCGGGGGAAGAAGATGGCAGCCGCCCAGTCCCACTCTCTGCAAGGG400.23181686467690527No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG400.23181686467690527Illumina Single End Adapter 2 (100% over 23bp)
AACGCCGGGTGGTAAATAAAAGATAAACTAGGAATTAAATTGCTATCTTA400.23181686467690527No Hit
AACGCCGGGAGATGTCTTTCCTGAGAGCTTTGGAGGTTGGGAATCATCTT390.22602144305998262No Hit
AACGCCGGGGTGCAGTGCTAACGCCGGGGAGCTGACTGTATTATTGAGTG390.22602144305998262No Hit
AACGCCGGGAGGGAGGTAATTCCCTTGGTAAAGTCTTATCCAGTTTGGAA390.22602144305998262No Hit
AACGCCGGGTACACTATTCACAGAGCTACATGTTAAGATGGAATATCTTT380.22022602144305997No Hit
AACGCCGGAGGTCGGTCCCGCGGCTCGGACCCCCCTGCTGCTGACGTGGA380.22022602144305997No Hit
AACGCCGGGAACCTCCTTAGCCCCACCCCTCAACCCTCACCACTCTATTT380.22022602144305997No Hit
AACGCCGGGAGCCGGAATGGCATGATACTGAAGCCCCACTTCCACAAGGA380.22022602144305997No Hit
AACGCCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCC380.22022602144305997No Hit
AACGCCGGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.19704433497536944No Hit
AACGCCGGCGTCGCTGTCTCGGCAATTCAACAAACAGCATTCAACGATGG330.19124891335844682No Hit
AACGCCGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.19124891335844682No Hit
AACGCCGGGATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAA330.19124891335844682No Hit
AACGCCGGGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1854534917415242No Hit
AACGCCGGATAAAATTGGATAGAACTACATTGGGCACAATAAAACCGAAG320.1854534917415242No Hit
AACGCCGGGAGGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTA310.17965807012460155No Hit
AACGCCGGGTTGCTCCTTGAAGATATCGCAGTGAAAGGAAAGAATGAAGA310.17965807012460155No Hit
AACGCCGGGGTGCTAACGCCGGGATTGCAAAATATAAAATAAATAAAAAA300.17386264850767894No Hit
AACGCCGGGAGTTAGAGTCATTATTATTTTATGCAAATATATGATTCTGA300.17386264850767894No Hit
AACGCCGGGCAGAGTGCAGTTCTAACGCCGGCACGCAGAGTGCAGTGCTA290.16806722689075632No Hit
AACGCCGGGATCTCCATTATTCTAGACCCACTAACAAAATATATAGCATA290.16806722689075632No Hit
AACGCCGGCCCCCAAACTCTTCTGAATGTACAAGTTAAAAAATTATGTAT290.16806722689075632No Hit
AACGCCGGGAGCCCCCCAGAATGGATTCTGGGCAGTGTCATTGTGGTATG290.16806722689075632No Hit
AACGCCGGGGCTGGGAGTCCTGGGAATGAGCAGAAAAAACATCAGCATAG290.16806722689075632No Hit
AACGCCGGGACGCCGGGCAGAGTGCAGTGCTAACGCCGGGATGTGGATTG290.16806722689075632No Hit
AACGCCGGGGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGC280.16227180527383367No Hit
AACGCCGGGGATGGTGAGCTTGTTCTCCTTTTAAAAAAAAAAAAAAAAAA280.16227180527383367No Hit
AACGCCGGGGAGTGGCCAATAAAATAGGAAAGAAAATAGTTCCTTAGAAT280.16227180527383367No Hit
AACGCCGGGGAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGCCGGGA280.16227180527383367No Hit
AACGCCGGGATGTCAGCGTCAACAACCCCATTTCTCCGGAGGTCGTCTTC270.15647638365691105No Hit
AACGCCGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.15647638365691105No Hit
AACGCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.15647638365691105No Hit
AACGCCGGGGAGTCCCTCTTGGTCCTCCTGACCTGTCCCCTGTGCATCCA260.1506809620399884No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGACGGGA260.1506809620399884No Hit
AACGCCGGGCTAAAACAAACCTTTGCCAGAAAATAAGGGTCACTCAGGGG260.1506809620399884No Hit
AACGCCGGGGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1506809620399884No Hit
AACGCCAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1506809620399884No Hit
AACGCCGAGGAAAATCAAAATGTCCATTAAGATGGCAAAATAACATCATA260.1506809620399884No Hit
AACGCCGGGAGTATGGAGATATTTTATTGTGCTATAGGTTACAATCATTG250.14488554042306578No Hit
AACGCCGGGCGCAGAGTGCAGTACTAACGCCGGGCAGGCTAGATCTTTTA240.13909011880614314No Hit
AACGCCGGGGCTTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGT240.13909011880614314No Hit
AACGCCGGGTCCCCCAACTCCTCTGAGATCCTCCCACCCCCACCCTCTTT240.13909011880614314No Hit
AACGCCGGGAATCTCAGAAAGAAGAAAACTGCATGATCTCCCTCATATGC240.13909011880614314No Hit
AACGCCGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.13329469718922052No Hit
AACGCCGGGGGCCTGGTGGGGTTGGCGGTGTGCGACACTCCACACGAGAG230.13329469718922052No Hit
AACGCCGGGAGAGTGCAGTGCTAACGCCGGAAGTCAACTCAGAGTGCAAT230.13329469718922052No Hit
AACGCCGGGGCCTTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.13329469718922052No Hit
AACGCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1274992755722979No Hit
AACGCCGGGGTTCCATGTGTAATGGTTAGCACTCTGGACTCTGAATCCAG220.1274992755722979No Hit
AACGCCGGGAATTTATATTTTAAATAAAATATTTTTAGAGTTTTTAATTT220.1274992755722979No Hit
AACGCCGGGATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCC220.1274992755722979No Hit
AACGCCGGGGTGCTAACGCCGGGGGTGACATTTTCATACCTGTATATAAT210.12170385395537525No Hit
AACGCCGGGTGCTAACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA190.11011301072152999No Hit
AACGCCGGGAAAACTCAGCAATTAAAAAAAAAAAAAAAAAAAAAAAAAAA190.11011301072152999No Hit
AACGCCGGAAGCAGTGATATCACGTGCAACGCCGGGGAAAAAAAAAAAAA190.11011301072152999No Hit
AACGCCGGGTGCTAACGCCGGAAGCAGTAGTATCAACGCAGAATGCAGTG190.11011301072152999No Hit
AACGCCGGGTGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGA180.10431758910460737No Hit
AACGCCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10431758910460737No Hit
AACGCCGGGGTGCAGTGCTAACGCCGGGAAGCAGTGGTAAGTGCAGTGCT180.10431758910460737No Hit
AACGCCGGGTGAGTTCAACATTTTAGCAGCATCCTTCACTTAAATAAAAA180.10431758910460737No Hit
AACGCCGGGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10431758910460737No Hit
AACGCCGGGATTGTACCATGTTGTTTTACATTACAATCCTGTGTTGGTTT180.10431758910460737No Hit
AACGCCGGGAGTGGTTAAGATGAATATACTAATGTTTAGAGTTACAAAAT180.10431758910460737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG207.6425605E-444.014
CTTTTGA407.525159E-944.041
CAGCTCC502.3646862E-1144.030
AGCCCGT207.6425605E-444.015
CTACAGC351.338467E-744.040
ATCCTGT454.2382453E-1044.039
AGCCTGT351.338467E-744.044
ACAGTGA454.2382453E-1044.039
ACAGCCT351.338467E-744.042
GCGGGGA207.6425605E-444.022
ATCCTCC207.6425605E-444.028
ATCCCAG207.6425605E-444.037
AGCCCAG302.3853227E-644.029
CTCTGAG207.6425605E-444.021
TGTTGGT351.338467E-744.024
GTAGAGT454.2382453E-1044.011
TTTTGAT407.525159E-944.042
CGTCACG302.3853227E-644.028
GGAGACC551.8189894E-1244.08
TGTTGAG207.6425605E-444.037
Produced by FastQC (version 0.11.5)