FastQCFastQC Report
Fri 17 Jun 2016
SRR1527458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13608
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5624.129923574368019No Hit
AACGCCGGGACCCCAATCCCTGATTCCCTCAGCTCCGCAGCGAGAGCGGTGC3462.5426219870664313No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3122.2927689594356258No Hit
AACGCCGGGAGACCTGGCATCACCCAACCACCAGTAGCATCCTGTGTGGGAT2772.035567313345091No Hit
AACGCCGGTAGTAGAGTTGGAGACAAGGTCCTACCTCCTCTGTGGGCTGCAA2762.0282186948853616No Hit
AACGCCGGGCATCCGGAACTTGTTTTAGCAGAGCGCTTGCCTGGTATAAATA2591.9032921810699588No Hit
AACGCCGGGATCTTGGTGTGGTATTGCCTACCTGCAATCCCAGAACTCAGGA2411.7710170487948267No Hit
AACGCCGGGGGGGGCCGCCCTCTCGCCCGTCACGTTGAACGCACGTTCGTGT2221.6313932980599646No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2131.5652557319223985No Hit
AACGCCGGGAACACCATACACAAAAATAAACTCAAAATGGATTAAAGAGCTC2051.506466784244562No Hit
AACGCCGGAAAGCAAGAAAGATGAGAGACAAAAGATAGACAGTGAGTATTGT1951.4329805996472662No Hit
AACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCG1811.3300999412110524No Hit
AACGCCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1751.286008230452675No Hit
AACGCCGGGGGATTTAGGGTGTTAGGTTGTTTTGTTTAATTATTCCATATTA1561.1463844797178129No Hit
AACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTA1561.1463844797178129No Hit
AACGCCGGGGGAGTCCAACTCTCACATCCATACATGACTACTGGAAAAACCA1491.094944150499706No Hit
AACGCCGGCTGTGATGTGTTTAATTTGAGGGCATCTCCTGACTTCTAGCTCC1381.0141093474426808No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGCCGGGAAG1360.9994121105232217No Hit
AACGCCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.9847148736037625No Hit
AACGCCGGGTGTGCGCCATAACTTCTGAAGCCCAGGTGCCTACAGCCTGTGC1300.9553203997648443No Hit
AACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGC1280.940623162845385No Hit
AACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACGATAAACC1270.9332745443856556No Hit
AACGCCGGGAAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAAGTGA1260.9259259259259258No Hit
AACGCCGGGGCGCCGAGAGCCGCGGGGAGAAGGCCATCACCCAGGTCAGCAA1230.9038800705467371No Hit
AACGCCGGGCCTTCGTGTTGACTTCATTCACAGGTTGGAGTTCGGAGAGGTG1200.8818342151675485No Hit
AACGCCGGGCCACGTGATCAGTGCGTGCACAGTAACATGATCAGTGCCTGTC1170.8597883597883598No Hit
AACGCCGGGGCATCAAGACTGACTGAAAAAATGCCGTGCGAAAAACATGATG1040.7642563198118754No Hit
AACGCCGGGAGGCATTCATCTCGAGTGGAAGCAAAGAACCTCGCTCTGCTCT970.7128159905937683No Hit
AACGCCGGGATGTCCTTGTAGGCTCTGTTTTAAGAAAACCATGTGGGAAATG920.6760728982951205No Hit
AACGCCGGGACTATTACAAAAAAAGAAAATGCTTAGAAAAGATTTAAGCAAT910.668724279835391No Hit
AACGCCGGGAACCTCTGTACTTGCAGACCATAGCAAATGTAGCTTTTAAACC880.6466784244562023No Hit
AACGCCGGGAATCAAAAAACCAAACAAACAAACAAACAAAAAATACCCATGC860.6319811875367431No Hit
AACGCCGGGATGACTACTTGTACTCTGCTTTTCCCGTGAACGTCAGCTCGGC840.6172839506172839No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.6099353321575544No Hit
AACGCCGGGGAACCCGGGTCTCCTACACTGCAGGCAGATTCTTTACTGTCTG820.6025867136978248No Hit
AACGCCGGGGGTGCTTAGGTGCCCGAGCTACTGAGGGTCTAAGTCCGGGCAG800.5878894767783657No Hit
AACGCCGGGGGCTCTTTTGCCTGCCACCAAAGACATGACTTTGCTCCTCATT760.5584950029394474No Hit
AACGCCGGGATTCCTCTTCTTCCTCTTCTTCCTCTTCTTCCCCTTCTTCTTC730.5364491475602586No Hit
AACGCCGGGAACTATTTGTAACTAGTTTCAGGGCATCTAACACCTCTTCTGA690.5070546737213404No Hit
AACGCCGGGGGACCGTCCTGGCTGCCAGTTACACTGCGATTGTAACAACATT680.4997060552616108No Hit
AACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGG650.47766019988242214No Hit
AACGCCGGGGTGCCGCTGGCCCGTCAGGCCCCAGCTGCCTCCTGTGCATGTG640.4703115814226925No Hit
AACGCCGGGAGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGC640.4703115814226925No Hit
AACGCCGGGGATCCATGCCCTTGGTGGCACCGTTCTCCTAGCATCTCAGATG630.4629629629629629No Hit
AACGCCGGGAGTACCCACCTGGTCGTGGGGGTCCTCCTCCACCTATGGGCCG620.4556143445032334No Hit
AACGCCGGGATGGAGAGGGAGCTGGAAGGCCAAGGTGGGGCTAAGGAACCAG610.44826572604350384No Hit
AACGCCGGGCTCTACTTTGTGCTGAGTGGTTGCAATGGCGGACGTGTCTGAG600.4409171075837742No Hit
AACGCCGGGCACAGTGGAGAGCCCGGCAGAGCAAAGCCTGAGACTGAACACA590.4335684891240447No Hit
AACGCCGGGATCTCTGTGGATCTTAGCCTCCCTTGAAGGGCAAGCCTGCAAG570.41887125220458554No Hit
AACGCCGGGAGGCTAACCTCAGAAGCATCAAATTCTGGTACACAAAACAGAG570.41887125220458554No Hit
AACGCCGGGAGCAAAATTACAGTCATGAAGTAGCAATGAAAATAATTTTATG560.411522633744856No Hit
AACGCCGGGATGGTCAAGTTTAAGGAGAATCAGATCTGTTGAGGTAACCTTG490.360082304526749No Hit
AACGCCGGGGAGCGATGCCACTGGACAGAAAAAAATTTACAATAATCCAGGC480.3527336860670194No Hit
AACGCCGGGAACCATGAAGAGAGAAACTGTCATTATATTACAAGAAGAAAAT480.3527336860670194No Hit
AACGCCGGGACAGCGTTTTCGTGTCCTGAAATCGCTTTATGCTGCAAAAGCG480.3527336860670194No Hit
AACGCCGGGGTGAGGTGTTTGCATGTGGCCATTCCCGTCACTGGCCTGTGAC480.3527336860670194No Hit
AACGCCGGGGCTGGTTCTCCAGCACTTCTGCACCTCGGGAGCCTGCCCAAGT480.3527336860670194No Hit
AACGCCGGGCAGTGCTAACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA460.33803644914756026No Hit
AACGCCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTC440.32333921222810114No Hit
AACGCCGGGATCACCGTGTCTCAGGATGAGGGTGTCCGTCCCAGCACCACCA440.32333921222810114No Hit
AACGCCGGGAGACCATAGCAAAAATCAACAAAGCCAAAAGCTGGTTCTTTGA440.32333921222810114No Hit
AACGCCGGGATGCATAGTGCTCCTACACTTAGGATGAGAATGATCTTCTTCT430.3159905937683716No Hit
AACGCCGGGTACACTATTCACAGAGCTACATGTTAAGATGGAATATCTTTTA420.30864197530864196No Hit
AACGCCGGGGTGCAGTGCTAACGCCGGGGAGCTGACTGTATTATTGAGTGTA410.3012933568489124No Hit
AACGCCGGGCAGATGGTACTCTGTATGTCATGGACATGTTTGTACCTGTACA400.29394473838918284No Hit
AACGCCGGGTGCAGTGCTAACGCCGGGGGGTAGTGGTTTTTAAAGAACTTGA390.2865961199294532No Hit
AACGCCGGGAACCTCCTTAGCCCCACCCCTCAACCCTCACCACTCTATTTTA390.2865961199294532No Hit
AACGCCGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.2865961199294532No Hit
AACGCCGGGGAAGCCACTGTTGTTGGCTGTGTGACAGTGCGTGTGTTATTGA380.2792475014697237No Hit
AACGCCGGGTTTTCTCTCTCTTGCTTCTTGCTCTCTTTTCCTGAAGATGTAA380.2792475014697237No Hit
AACGCCGGGTCCCCCAACTCCTCTGAGATCCTCCCCCCCCCCCCCCCTTTTT380.2792475014697237No Hit
AACGCCGGGATGGATGGTAGTAATAAAGATTGTTTATCATTCACAAAGCTAT360.26455026455026454No Hit
AACGCCGGGACGATGTGAAAGCTCCAGCCATGTTCAATATTAGGAACATCGG360.26455026455026454No Hit
AACGCCGGGGGGGAAACCCGTAGCCCTTGCAGCATGGCTGACCAACTGACTG360.26455026455026454No Hit
AACGCCGGGAGATAAGGAGCAACGGCTATGCTTTGCTGGAGCAGCCGTGAAG360.26455026455026454No Hit
AACGCCGGGGGCCGGGTGGCGACTGGCTCCCGGGTCTGAGGGGCTTCTGCTT350.257201646090535No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGCCGGGGAG350.257201646090535No Hit
AACGCCGGGTCCCCCAACTCCTCTGAGATCCTCCCCCCCCCCCCCCCTTTCT350.257201646090535No Hit
AACGCCGGGGCTGGGAGTCCTGGGAATGAGCAGAAAAAACATCAGCATAGAG340.2498530276308054No Hit
AACGCCGGCCCCCAAACTCTTCTGAATGTACAAGTTAAAAAATTATGTATTA330.24250440917107582No Hit
AACGCCGGGGGAAGAAGATGGCAGCCGCCCAGTCCCACTCTCTGCAAGGGTT330.24250440917107582No Hit
AACGCCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA320.23515579071134626No Hit
AACGCCGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.2278071722516167No Hit
AACGCCGGGAGCCGGAATGGCATGATACTGAAGCCCCACTTCCACAAGGATT310.2278071722516167No Hit
AACGCCGGGTGGTAAATAAAAGATAAACTAGGAATTAAATTGCTATCTTATG300.2204585537918871No Hit
AACGCCGGGAATCTCAGAAAGAAGAAAACTGCATGATCTCCCTCATATGCAG300.2204585537918871No Hit
AACGCCGGGTGGGATGAGCCCTGCACGGACGGCATTGGGTGGAAAAGGCCAA290.21310993533215755No Hit
AACGCCGGGGCTTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTC280.205761316872428No Hit
AACGCCGGGGTTCCATGTGTAATGGTTAGCACTCTGGACTCTGAATCCAGCG280.205761316872428No Hit
AACGCCGGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.205761316872428No Hit
AACGCCGGGAGGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTACA280.205761316872428No Hit
AACGCCGGAGGTCGGTCCCGCGGCTCGGACCCCCCTGCTGCTGACGTGGAGA270.1984126984126984No Hit
AACGCCGGGCAGAGTGCAGTTCTAACGCCGGCACGCAGAGTGCAGTGCTAAC270.1984126984126984No Hit
AACGCCGGGGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGCAA270.1984126984126984No Hit
AACGCCGGGCTAAAACAAACCTTTGCCAGAAAATAAGGGTCACTCAGGGGAA270.1984126984126984No Hit
AACGCCGGGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.19106407995296884No Hit
AACGCCGGGACGCCGGGCAGAGTGCAGTGCTAACGCCGGGATGTGGATTGTC260.19106407995296884No Hit
AACGCCGGGGATATTATATTTTACAACCTCCAATGAAATAATGTATGCTGGT260.19106407995296884No Hit
AACGCCGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.18371546149323928No Hit
AACGCCGGGAGCCCCCCAGAATGGATTCTGGGCAGTGTCATTGTGGTATGGG250.18371546149323928No Hit
AACGCCGGGAGATGTCTTTCCTGAGAGCTTTGGAGGTTGGGAATCATCTTCT250.18371546149323928No Hit
AACGCCGGGGTGCTAACGCCGGGGGTGACATTTTCATACCTGTATATAATGA240.1763668430335097No Hit
AACGCCGGATAAAATTGGATAGAACTACATTGGGCACAATAAAACCGAAGAT240.1763668430335097No Hit
AACGCCGGCGTCGCTGTCTCGGCAATTCAACAAACAGCATTCAACGATGGCT230.16901822457378013No Hit
AACGCCGAGGAAAATCAAAATGTCCATTAAGATGGCAAAATAACATCATATT220.16166960611405057No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC210.15432098765432098Illumina Single End Adapter 2 (100% over 25bp)
AACGCCAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.15432098765432098No Hit
AACGCCGGGGAGTCCCTCTTGGTCCTCCTGACCTGTCCCCTGTGCATCCAGA210.15432098765432098No Hit
AACGCCGGGGAGTGGCCAATAAAATAGGAAAGAAAATAGTTCCTTAGAATTT210.15432098765432098No Hit
AACGCCGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14697236919459142No Hit
AACGCCGGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14697236919459142No Hit
AACGCCGGGGTGCTAACGCCGGGATTGCAAAATATAAAATAAATAAAAAAAG200.14697236919459142No Hit
AACGCCGGGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14697236919459142No Hit
AACGCCGGGAGTATGGAGATATTTTATTGTGCTATAGGTTACAATCATTGTA190.13962375073486186No Hit
AACGCCGGGATGTCAGCGTCAACAACCCCATTTCTCCGGAGGTCGTCTTCAC190.13962375073486186No Hit
AACGCCGGGGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.13962375073486186No Hit
AACGCCGGGATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAA190.13962375073486186No Hit
AACGCCGGGGGCCTGGTGGGGTTGGCGGTGTGCGACACTCCACACGAGAGGC180.13227513227513227No Hit
AACGCCGGGGATGGTGAGCTTGTTCTCCTTTTAAAAAAAAAAAAAAAAAAAA180.13227513227513227No Hit
AACGCCGGGTTGCTCCTTGAAGATATCGCAGTGAAAGGAAAGAATGAAGAAA180.13227513227513227No Hit
AACGCCGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.13227513227513227No Hit
AACGCCGGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.13227513227513227No Hit
AACGCCGGGAGGGAGGTAATTCCCTTGGTAAAGTCTTATCCAGTTTGGAATT180.13227513227513227No Hit
AACGCCGAGGAAAAAAAAAAGAAAAAAAGGAAAGAAAAAAAGAGAAAAAAAA170.1249265138154027No Hit
AACGCCGGGGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTA170.1249265138154027No Hit
AACGCCGGGGTGCAGTGCTAACGCCGGGAAGCAGTGGTAAGTGCAGTGCTAA170.1249265138154027No Hit
AACGCCGGGAAAACTCAGCAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.1249265138154027No Hit
AACGCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11757789535567313No Hit
AACGCCGGGAGTTAGAGTCATTATTATTTTATGCAAATATATGATTCTGAAA160.11757789535567313No Hit
AACGCCGGGAATTTATATTTTAAATAAAATATTTTTAGAGTTTTTAATTTTA160.11757789535567313No Hit
AACGCCGGGTGCTAACGCCGGAAGCAGTAGTATCAACGCAGAATGCAGTGCT160.11757789535567313No Hit
AACGCCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11757789535567313No Hit
AACGCCGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTA150.11022927689594356No Hit
AACGCCGGGCGCAGAGTGCAGTACTAACGCCGGGCAGGCTAGATCTTTTATT150.11022927689594356No Hit
AACGCCGGGAAACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.11022927689594356No Hit
AACGCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.11022927689594356No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA150.11022927689594356No Hit
AACGCCGGGACAGTGCAGACAGCAGATCCTTTCACAGGTGCTGGGCGTTACA150.11022927689594356No Hit
AACGCCGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTC150.11022927689594356No Hit
AACGCCGGGGTGCTAACGCCGGGACCCCAGTTTCAGGGTCCTCCCTCCTGAG140.102880658436214No Hit
AACGCCGGGATCTCCATTATTCTAGACCCACTAACAAAATATATAGCATACC140.102880658436214No Hit
AACGCCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.102880658436214No Hit
AACGCCGGGTACTTCATTCTTACATCAACATTCACTTTAAAATCTATCGAAG140.102880658436214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATT206.0929725E-446.022
CTTTTGA301.728562E-646.041
CTACAGC206.0929725E-446.040
CAGCCAA206.0929725E-446.020
ATCCTGT253.2412205E-546.039
AGCCTGT206.0929725E-446.044
TGATGCG404.942194E-946.042
TGATGCC301.728562E-646.045
CTACACT253.2412205E-546.023
TTGTTTC301.728562E-646.017
ACAGCCT206.0929725E-446.042
ATCCCAG253.2412205E-546.037
AGCCCAG206.0929725E-446.029
TGTTGGT301.728562E-646.024
GTAGAGT253.2412205E-546.011
TTCATTC206.0929725E-446.023
TTTTGAT301.728562E-646.042
CGTCACG301.728562E-646.028
GGAGACC253.2412205E-546.08
GGAGACA253.2412205E-546.019
Produced by FastQC (version 0.11.5)