FastQCFastQC Report
Fri 17 Jun 2016
SRR1527457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18153
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7774.280284250537101No Hit
AACGCCGGTAGTAGAGTTGGAGACAAGGTCCTACCTCCTCTGTGGGCTGCAA4312.374263207183386No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4302.368754475844213No Hit
AACGCCGGGACCCCAATCCCTGATTCCCTCAGCTCCGCAGCGAGAGCGGTGC3962.181457610312345No Hit
AACGCCGGGAGACCTGGCATCACCCAACCACCAGTAGCATCCTGTGTGGGAT3792.087809177546411No Hit
AACGCCGGGCATCCGGAACTTGTTTTAGCAGAGCGCTTGCCTGGTATAAATA3621.994160744780477No Hit
AACGCCGGGAACACCATACACAAAAATAAACTCAAAATGGATTAAAGAGCTC3361.85093372996199No Hit
AACGCCGGGATCTTGGTGTGGTATTGCCTACCTGCAATCCCAGAACTCAGGA3081.6966892524651573No Hit
AACGCCGGGGGGGGCCGCCCTCTCGCCCGTCACGTTGAACGCACGTTCGTGT3041.6746543271084668No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2901.5975320883600506No Hit
AACGCCGGAAAGCAAGAAAGATGAGAGACAAAAGATAGACAGTGAGTATTGT2761.5204098496116345No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGCCGGGAAG2401.3220955214014212No Hit
AACGCCGGGGGAGTCCAACTCTCACATCCATACATGACTACTGGAAAAACCA2201.2119208946179694No Hit
AACGCCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2171.1953947006004517No Hit
AACGCCGGCTGTGATGTGTTTAATTTGAGGGCATCTCCTGACTTCTAGCTCC2081.1458161185478983No Hit
AACGCCGGGAAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAAGTGA2061.134798655869553No Hit
AACGCCGGGGGATTTAGGGTGTTAGGTTGTTTTGTTTAATTATTCCATATTA1921.057676417121137No Hit
AACGCCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.9364843276593401No Hit
AACGCCGGGGCGCCGAGAGCCGCGGGGAGAAGGCCATCACCCAGGTCAGCAA1700.9364843276593401No Hit
AACGCCGGGGCATCAAGACTGACTGAAAAAATGCCGTGCGAAAAACATGATG1500.8263097008758883No Hit
AACGCCGGGTGTGCGCCATAACTTCTGAAGCCCAGGTGCCTACAGCCTGTGC1480.815292238197543No Hit
AACGCCGGGATGACTACTTGTACTCTGCTTTTCCCGTGAACGTCAGCTCGGC1470.8097835068583705No Hit
AACGCCGGGAACCTCTGTACTTGCAGACCATAGCAAATGTAGCTTTTAAACC1450.7987660441800253No Hit
AACGCCGGGCCTTCGTGTTGACTTCATTCACAGGTTGGAGTTCGGAGAGGTG1410.776731118823335No Hit
AACGCCGGGCCACGTGATCAGTGCGTGCACAGTAACATGATCAGTGCCTGTC1300.7161350740924365No Hit
AACGCCGGGAATCAAAAAACCAAACAAACAAACAAACAAAAAATACCCATGC1280.7051176114140913No Hit
AACGCCGGGATGTCCTTGTAGGCTCTGTTTTAAGAAAACCATGTGGGAAATG1260.6941001487357461No Hit
AACGCCGGGAGGCATTCATCTCGAGTGGAAGCAAAGAACCTCGCTCTGCTCT1240.683082686057401No Hit
AACGCCGGGACTATTACAAAAAAAGAAAATGCTTAGAAAAGATTTAAGCAAT1180.6500302980223654No Hit
AACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCG1150.6335041040048477No Hit
AACGCCGGGGAACCCGGGTCTCCTACACTGCAGGCAGATTCTTTACTGTCTG1140.6279953726656751No Hit
AACGCCGGGGGTGCTTAGGTGCCCGAGCTACTGAGGGTCTAAGTCCGGGCAG1130.6224866413265026No Hit
AACGCCGGGGGCTCTTTTGCCTGCCACCAAAGACATGACTTTGCTCCTCATT1070.5894342532914669No Hit
AACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACGATAAACC1030.5673993279347765No Hit
AACGCCGGGAGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGC990.5453644025780863No Hit
AACGCCGGGAGTACCCACCTGGTCGTGGGGGTCCTCCTCCACCTATGGGCCG940.5178207458822234No Hit
AACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTA920.5068032832038781No Hit
AACGCCGGGCTCTACTTTGTGCTGAGTGGTTGCAATGGCGGACGTGTCTGAG900.495785820525533No Hit
AACGCCGGGAACTATTTGTAACTAGTTTCAGGGCATCTAACACCTCTTCTGA900.495785820525533No Hit
AACGCCGGGATGGAGAGGGAGCTGGAAGGCCAAGGTGGGGCTAAGGAACCAG860.47375089516884267No Hit
AACGCCGGGGATCCATGCCCTTGGTGGCACCGTTCTCCTAGCATCTCAGATG840.4627334324904975No Hit
AACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGC840.4627334324904975No Hit
AACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGG790.43518977579463447No Hit
AACGCCGGGGTGCCGCTGGCCCGTCAGGCCCCAGCTGCCTCCTGTGCATGTG790.43518977579463447No Hit
AACGCCGGGGGACCGTCCTGGCTGCCAGTTACACTGCGATTGTAACAACATT760.4186635817771167No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGCCGGGGAG750.41315485043794414No Hit
AACGCCGGGATGCATAGTGCTCCTACACTTAGGATGAGAATGATCTTCTTCT740.4076461190987715No Hit
AACGCCGGGATCTCTGTGGATCTTAGCCTCCCTTGAAGGGCAAGCCTGCAAG730.40213738775959895No Hit
AACGCCGGGGAAGCCACTGTTGTTGGCTGTGTGACAGTGCGTGTGTTATTGA720.39662865642042633No Hit
AACGCCGGGAGCAAAATTACAGTCATGAAGTAGCAATGAAAATAATTTTATG710.3911199250812538No Hit
AACGCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.3801024624029086No Hit
AACGCCGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.37459373106373606No Hit
AACGCCGGGATTCCTCTTCTTCCTCTTCTTCCTCTTCTTCCCCTTCTTCTTC680.37459373106373606No Hit
AACGCCGGGATCACCGTGTCTCAGGATGAGGGTGTCCGTCCCAGCACCACCA640.3525588057070457No Hit
AACGCCGGGCAGATGGTACTCTGTATGTCATGGACATGTTTGTACCTGTACA620.3415413430287005No Hit
AACGCCGGGTACACTATTCACAGAGCTACATGTTAAGATGGAATATCTTTTA610.3360326116895279No Hit
AACGCCGGGGCTGGTTCTCCAGCACTTCTGCACCTCGGGAGCCTGCCCAAGT610.3360326116895279No Hit
AACGCCGGGGGGGAAACCCGTAGCCCTTGCAGCATGGCTGACCAACTGACTG610.3360326116895279No Hit
AACGCCGGGCACAGTGGAGAGCCCGGCAGAGCAAAGCCTGAGACTGAACACA600.3305238803503553No Hit
AACGCCGGGGTGAGGTGTTTGCATGTGGCCATTCCCGTCACTGGCCTGTGAC600.3305238803503553No Hit
AACGCCGGGATGGTCAAGTTTAAGGAGAATCAGATCTGTTGAGGTAACCTTG590.3250151490111827No Hit
AACGCCGGCCCCCAAACTCTTCTGAATGTACAAGTTAAAAAATTATGTATTA590.3250151490111827No Hit
AACGCCGGGGAGCGATGCCACTGGACAGAAAAAAATTTACAATAATCCAGGC580.31950641767201016No Hit
AACGCCGGGATGGATGGTAGTAATAAAGATTGTTTATCATTCACAAAGCTAT580.31950641767201016No Hit
AACGCCGGGAACCATGAAGAGAGAAACTGTCATTATATTACAAGAAGAAAAT570.31399768633283753No Hit
AACGCCGGGACAGCGTTTTCGTGTCCTGAAATCGCTTTATGCTGCAAAAGCG510.280945298297802No Hit
AACGCCGGGTTGCTCCTTGAAGATATCGCAGTGAAAGGAAAGAATGAAGAAA510.280945298297802No Hit
AACGCCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTC510.280945298297802No Hit
AACGCCGGGCAGTGCTAACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA510.280945298297802No Hit
AACGCCGGGTGGGATGAGCCCTGCACGGACGGCATTGGGTGGAAAAGGCCAA510.280945298297802No Hit
AACGCCGGGAACCTCCTTAGCCCCACCCCTCAACCCTCACCACTCTATTTTA490.2699278356194568No Hit
AACGCCGGGTGGTAAATAAAAGATAAACTAGGAATTAAATTGCTATCTTATG490.2699278356194568No Hit
AACGCCGGGATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAA480.26441910428028426No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC470.2589103729411117Illumina Single End Adapter 2 (100% over 25bp)
AACGCCGGGTGCAGTGCTAACGCCGGGGGGTAGTGGTTTTTAAAGAACTTGA460.25340164160193906No Hit
AACGCCGGGGATATTATATTTTACAACCTCCAATGAAATAATGTATGCTGGT460.25340164160193906No Hit
AACGCCGGGAGGCTAACCTCAGAAGCATCAAATTCTGGTACACAAAACAGAG460.25340164160193906No Hit
AACGCCGGGGGCCGGGTGGCGACTGGCTCCCGGGTCTGAGGGGCTTCTGCTT450.2478929102627665No Hit
AACGCCGGGAGATGTCTTTCCTGAGAGCTTTGGAGGTTGGGAATCATCTTCT450.2478929102627665No Hit
AACGCCGGGACGATGTGAAAGCTCCAGCCATGTTCAATATTAGGAACATCGG440.2423841789235939No Hit
AACGCCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA430.23687544758442133No Hit
AACGCCGGGAGGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTACA430.23687544758442133No Hit
AACGCCGGGGTGCAGTGCTAACGCCGGGGAGCTGACTGTATTATTGAGTGTA420.23136671624524874No Hit
AACGCCGGGAGATAAGGAGCAACGGCTATGCTTTGCTGGAGCAGCCGTGAAG420.23136671624524874No Hit
AACGCCGGGAATCTCAGAAAGAAGAAAACTGCATGATCTCCCTCATATGCAG410.22585798490607614No Hit
AACGCCGGGGGAAGAAGATGGCAGCCGCCCAGTCCCACTCTCTGCAAGGGTT410.22585798490607614No Hit
AACGCCGGGAGACCATAGCAAAAATCAACAAAGCCAAAAGCTGGTTCTTTGA410.22585798490607614No Hit
AACGCCGGGATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAA400.22034925356690352No Hit
AACGCCGGGAGCCCCCCAGAATGGATTCTGGGCAGTGTCATTGTGGTATGGG390.21484052222773092No Hit
AACGCCGGGGTGCTAACGCCGGGATTGCAAAATATAAAATAAATAAAAAAAG390.21484052222773092No Hit
AACGCCGGGGAGTGGCCAATAAAATAGGAAAGAAAATAGTTCCTTAGAATTT390.21484052222773092No Hit
AACGCCAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.20382305954938576No Hit
AACGCCGGGAGTATGGAGATATTTTATTGTGCTATAGGTTACAATCATTGTA360.19831432821021316No Hit
AACGCCGGATAAAATTGGATAGAACTACATTGGGCACAATAAAACCGAAGAT360.19831432821021316No Hit
AACGCCGGGAGGGAGGTAATTCCCTTGGTAAAGTCTTATCCAGTTTGGAATT350.1928055968710406No Hit
AACGCCGGGTCCCCCAACTCCTCTGAGATCCTCCCCCCCCCCCCCCCCTTTT350.1928055968710406No Hit
AACGCCGGGATGTCAGCGTCAACAACCCCATTTCTCCGGAGGTCGTCTTCAC340.18729686553186803No Hit
AACGCCGGGTGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAAC340.18729686553186803No Hit
AACGCCGGAGGTCGGTCCCGCGGCTCGGACCCCCCTGCTGCTGACGTGGAGA310.17077067151435024No Hit
AACGCCGGGAGCCGGAATGGCATGATACTGAAGCCCCACTTCCACAAGGATT310.17077067151435024No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA310.17077067151435024No Hit
AACGCCGGGTTTTCTCTCTCTTGCTTCTTGCTCTCTTTTCCTGAAGATGTAA310.17077067151435024No Hit
AACGCCGGGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.16526194017517765No Hit
AACGCCGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.15975320883600508No Hit
AACGCCGGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.15975320883600508No Hit
AACGCCGGGCTAAAACAAACCTTTGCCAGAAAATAAGGGTCACTCAGGGGAA290.15975320883600508No Hit
AACGCCGGGGCTTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTC280.15424447749683248No Hit
AACGCCGGGAGTTAGAGTCATTATTATTTTATGCAAATATATGATTCTGAAA280.15424447749683248No Hit
AACGCCGGGGTTCCATGTGTAATGGTTAGCACTCTGGACTCTGAATCCAGCG280.15424447749683248No Hit
AACGCCGGAGACGCCTTGATTATAGCTCAGTAAGACCAATGTCAAACTTCTA280.15424447749683248No Hit
AACGCCGGGGGCCTGGTGGGGTTGGCGGTGTGCGACACTCCACACGAGAGGC270.1487357461576599No Hit
AACGCCGGGGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.1487357461576599No Hit
AACGCCGGGGAGTCCCTCTTGGTCCTCCTGACCTGTCCCCTGTGCATCCAGA270.1487357461576599No Hit
AACGCCGGGGCTGGGAGTCCTGGGAATGAGCAGAAAAAACATCAGCATAGAG260.1432270148184873No Hit
AACGCCGGGACAGTGCAGACAGCAGATCCTTTCACAGGTGCTGGGCGTTACA250.13771828347931472No Hit
AACGCCGGCGTCGCTGTCTCGGCAATTCAACAAACAGCATTCAACGATGGCT250.13771828347931472No Hit
AACGCCGGGCAGAGTGCAGTTCTAACGCCGGCACGCAGAGTGCAGTGCTAAC240.13220955214014213No Hit
AACGCCGGGATCTCCATTATTCTAGACCCACTAACAAAATATATAGCATACC240.13220955214014213No Hit
AACGCCGGGTCCCCCAACTCCTCTGAGATCCTCCCCCCCCCCCCCCCTTTTT240.13220955214014213No Hit
AACGCCGGGGATGGTGAGCTTGTTCTCCTTTTAAAAAAAAAAAAAAAAAAAA230.12670082080096953No Hit
AACGCCGGGCGCAGAGTGCAGTACTAACGCCGGGCAGGCTAGATCTTTTATT230.12670082080096953No Hit
AACGCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.12670082080096953No Hit
AACGCCGGGGTGCTAACGCCGGGGGTGACATTTTCATACCTGTATATAATGA230.12670082080096953No Hit
AACGCCGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTC230.12670082080096953No Hit
AACGCCGGGATTGTACCATGTTGTTTTACATTACAATCCTGTGTTGGTTTAC220.12119208946179695No Hit
AACGCCGGGAGCCGGAATGGCATGATACTGAAGCTCCACTTCCACAAGGATT220.12119208946179695No Hit
AACGCCGGGGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGCAA220.12119208946179695No Hit
AACGCCGGGAAAACTCAGCAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12119208946179695No Hit
AACGCCGGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11568335812262437No Hit
AACGCCGGGGGGCAAGAGAGATGACTCAGCCGTTAAAGGCTAGGCTCACAAC210.11568335812262437No Hit
AACGCCGGAAGCAGTGATATCACGTGCAACGCCGGGGAAAAAAAAAAAAAAA210.11568335812262437No Hit
AACGCCGGGAATTTATATTTTAAATAAAATATTTTTAGAGTTTTTAATTTTA210.11568335812262437No Hit
AACGCCGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11017462678345176No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAACGCCGGGCAG200.11017462678345176No Hit
AACGCCGGGGTGCAGTGCTAACGCCGGGAAGCAGTGGTAAGTGCAGTGCTAA200.11017462678345176No Hit
AACGCCGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTA190.10466589544427918No Hit
AACGCCGGGATTGCTGGTCTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10466589544427918No Hit
AACGCCGGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10466589544427918No Hit
AACGCCGAGGAAAATCAAAATGTCCATTAAGATGGCAAAATAACATCATATT190.10466589544427918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG253.2852065E-546.014
GCCCCAG253.2852065E-546.028
CTTCATT206.1479054E-446.022
TTAGAAT206.1479054E-446.044
AGGGGTT301.7615421E-646.038
TTAGAAA206.1479054E-446.033
CAGCTCC452.746674E-1046.030
CTACAGC301.7615421E-646.040
ATCCTGT253.2852065E-546.039
AGCCTGT301.7615421E-646.044
CGCTGGC253.2852065E-546.014
ACAGTGA206.1479054E-446.039
ACAGCCT301.7615421E-646.042
GGATGTC253.2852065E-546.08
ATCCCAG501.4551915E-1146.037
AGCCCAG301.7615421E-646.029
TAGAAAA206.1479054E-446.034
CGCTGAG301.7615421E-646.033
ACAGTAA206.1479054E-446.029
GTAGAGT253.2852065E-546.011
Produced by FastQC (version 0.11.5)