SRR1527456_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3187870
Sequences flagged as poor quality	0
Sequence length	50
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	79750	2.5016703943385394	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	42281	1.3263087892542669	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	29255	0.9176973966943445	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	26601	0.8344443154833792	No Hit
AAAACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	13686	0.4293148716854828	No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	8633	0.2708077807438823	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	7743	0.24288945283214183	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC	7617	0.23893697045362575	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	6929	0.21735516191061743	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5955	0.18680184574653294	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	5893	0.18485697346504093	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	5097	0.1598873228833045	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5075	0.1591972069124525	No Hit
AAAACGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT	5033	0.1578797127862805	No Hit
AAAACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC	5026	0.15766013043191848	No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4963	0.15568388924266047	No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	4839	0.1517941446796764	No Hit
AAAACGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	4832	0.15157456232531438	No Hit
AAAACGGGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAG	4770	0.14962969004382237	No Hit
AAAACGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAG	4505	0.14131692948583224	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	4459	0.13987395972859623	No Hit
AAAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4374	0.13720760256848616	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	4369	0.13705075802965616	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	4044	0.126855863005706	No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	4028	0.12635396048145	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG	3944	0.12371897222910595	No Hit
AAAACGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG	3842	0.12051934363697389	No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	3726	0.11688055033611784	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	3591	0.11264574778770778	No Hit
AAAACGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3478	0.10910106121014972	No Hit
AAAACGGGGGGGGACTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTG	3446	0.1080972561616377	No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3402	0.10671702421993369	No Hit
AAAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	3378	0.10596417043354966	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	3328	0.10439572504524965	No Hit
AAAACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	3273	0.10267043511811962	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AAAACGG	324625	43.000385	1
CGGGGAT	24900	42.992767	5
AAACGGG	322230	42.99091	2
AACGGGG	301400	42.986862	3
ACGGGGG	173495	42.95259	4
CGGGGGA	33735	42.80006	5
CGGGGGG	90890	42.782486	5
ACGGGGC	24695	42.619152	4
AACGGGT	6510	42.479263	3
ACGGGGA	91740	42.441246	4
ACGGGTA	2255	42.341465	4
CGGGGAG	33790	42.248592	5
CGGGGGT	23000	42.067825	5
ACGGGGT	13275	42.044445	4
CGGGGCA	8615	41.829365	5
ACGGGAT	2295	41.795208	4
AACGGGC	3905	41.74648	3
CGGGGGC	27725	41.555992	5
AACGGGA	11050	41.371944	3
ACGGGAG	4940	41.2834	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content