##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3187870
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92227192451386	31.0	31.0	33.0	28.0	34.0
2	31.609994761392404	31.0	31.0	34.0	30.0	34.0
3	32.17942889766521	33.0	31.0	34.0	31.0	34.0
4	36.12946669719907	37.0	35.0	37.0	35.0	37.0
5	36.290154240919485	37.0	37.0	37.0	35.0	37.0
6	36.25294224670391	37.0	37.0	37.0	35.0	37.0
7	36.634726008275116	37.0	37.0	37.0	35.0	37.0
8	36.69100120142917	37.0	37.0	37.0	35.0	37.0
9	38.410198031914724	39.0	39.0	39.0	37.0	39.0
10	37.16503558802586	39.0	37.0	39.0	34.0	39.0
11	35.90887551876331	37.0	35.0	39.0	33.0	39.0
12	35.99145197263376	37.0	35.0	39.0	33.0	39.0
13	36.03869354772936	37.0	35.0	39.0	33.0	39.0
14	36.65569486836038	39.0	35.0	40.0	33.0	40.0
15	36.79112604968208	39.0	35.0	40.0	33.0	40.0
16	36.95900679764231	39.0	35.0	40.0	33.0	40.0
17	36.846636782553865	38.0	35.0	40.0	33.0	40.0
18	36.7402839513531	38.0	35.0	40.0	33.0	40.0
19	36.67072026149121	38.0	35.0	40.0	33.0	40.0
20	36.49833682051025	38.0	35.0	40.0	32.0	40.0
21	36.38755093526399	38.0	35.0	40.0	32.0	40.0
22	36.227174571108606	38.0	35.0	40.0	32.0	40.0
23	36.063855489715706	37.0	34.0	40.0	32.0	40.0
24	35.939188549093906	37.0	34.0	39.0	32.0	40.0
25	35.68353257817916	36.0	34.0	39.0	31.0	40.0
26	35.15175493354497	36.0	34.0	38.0	30.0	40.0
27	35.05779815362609	36.0	34.0	38.0	30.0	40.0
28	34.86503809753848	35.0	34.0	38.0	30.0	40.0
29	34.50447351993651	35.0	33.0	38.0	30.0	39.0
30	34.136828038784515	35.0	33.0	38.0	29.0	39.0
31	33.94305445328699	35.0	33.0	38.0	29.0	39.0
32	33.84106629191278	35.0	33.0	38.0	28.0	39.0
33	33.24192862318727	34.0	32.0	37.0	26.0	39.0
34	32.46702500415638	34.0	31.0	37.0	23.0	38.0
35	32.320254903744505	34.0	31.0	36.0	23.0	38.0
36	32.142908901554954	34.0	31.0	36.0	23.0	38.0
37	32.01742229137324	34.0	31.0	36.0	24.0	38.0
38	31.50491707629232	33.0	30.0	35.0	23.0	38.0
39	30.779639069347244	33.0	29.0	35.0	22.0	38.0
40	30.56528748035522	32.0	29.0	34.0	21.0	37.0
41	30.203253269424412	32.0	28.0	34.0	21.0	37.0
42	29.768277250954398	32.0	27.0	34.0	21.0	36.0
43	29.151085521053243	31.0	27.0	34.0	19.0	36.0
44	28.499584989350257	30.0	26.0	34.0	18.0	36.0
45	27.96091465461264	30.0	23.0	33.0	18.0	35.0
46	27.976599735873798	30.0	23.0	33.0	19.0	35.0
47	27.51368594076923	29.0	23.0	33.0	18.0	35.0
48	27.09227258326092	29.0	23.0	32.0	18.0	34.0
49	26.878957736670568	29.0	23.0	32.0	18.0	34.0
50	30.343921489897642	32.0	29.0	34.0	22.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	12.0
13	27.0
14	90.0
15	263.0
16	514.0
17	891.0
18	1765.0
19	2773.0
20	4379.0
21	6726.0
22	9987.0
23	14221.0
24	19522.0
25	26875.0
26	36169.0
27	49547.0
28	67012.0
29	93209.0
30	131244.0
31	200026.0
32	351566.0
33	472695.0
34	444069.0
35	667773.0
36	521296.0
37	63731.0
38	1485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.53025060620413	0.35001427285303355	0.0696703441482871	0.05006477679453679
8	99.21922788570426	0.6063296182090235	0.10778356708397771	0.06665892900275106
9	93.47730616367669	3.2736278455520456	2.0085825331647778	1.2404834576064896
10	57.04103366824871	29.77997848092927	5.007199164332297	8.171788686489725
11	43.50889465379705	20.403090464793106	19.48391872943376	16.604096151976083
12	38.02084150231973	15.483253708589121	22.890644850636946	23.605259938454203
13	27.57066630696986	15.399373249222837	25.51989259285981	31.5100678509475
14	23.43194672304705	17.67339947990351	31.97846838170942	26.91618541534002
15	29.404116228077054	18.01836335860622	28.027272128411763	24.550248284904967
16	37.23536405185908	18.80384080906687	22.417444876986828	21.543350262087223
17	33.08070906279114	20.11496704696239	24.43923372032109	22.365090169925374
18	33.144387945556126	20.36776907464859	22.790107501246915	23.697735478548374
19	29.863074717601407	26.021638272576986	20.6640170395907	23.451269970230907
20	32.79823204835831	25.38252814575249	20.288374369092843	21.530865436796358
21	30.18401001295536	25.792802090424015	22.288204976990905	21.734982919629722
22	26.76075247735949	22.20297565459068	28.121441589525293	22.91483027852453
23	30.904961620141346	20.265976968947918	26.28247074065128	22.54659067025945
24	34.541935524346975	21.010517994773938	20.45092804913626	23.996618431742824
25	30.315947639019157	20.61489333002914	20.100882407375458	28.968276623576244
26	24.556522066458168	20.475552641732566	25.56214651162061	29.40577878018865
27	24.142201532684833	18.744428097758064	24.762898110650685	32.350472258906414
28	25.153064585444202	25.36552619774332	20.68393629602211	28.797472920790373
29	31.038875487394403	24.24935772161349	19.587718445231456	25.124048345760645
30	29.621753710157567	19.96345522245261	26.085285786434202	24.329505280955622
31	33.75645807388633	19.324909735967903	23.863677000630513	23.054955189515255
32	37.82764039938893	19.570402808144625	20.59679347024816	22.005163322218284
33	37.3350858096472	19.56648169467387	21.77450774341488	21.323924752264052
34	29.297618786211483	21.193304620326426	29.432191400527625	20.07688519293447
35	26.409452079288055	28.74681213474828	25.59348405047885	19.25025173548482
36	26.55214924071559	30.78770464291204	20.81085489684335	21.849291219529025
37	31.599908402789325	27.593659716362335	18.752082111252967	22.054349769595373
38	31.21874480452465	27.842258310407892	19.259881990168985	21.679114894898476
39	32.87533682364714	24.044299171547145	19.30094389043468	23.77942011437104
40	29.903321026265182	21.654082506501208	19.544052925621184	28.898543541612426
41	24.87118358025892	25.899550483551714	20.121146721792293	29.10811921439707
42	23.203110540894077	23.577655299620123	21.32370516990969	31.89552898957611
43	24.065849611182387	20.15091581526223	22.972768651168334	32.81046592238705
44	26.15203882216025	18.972981959741144	22.407187244147348	32.46779197395126
45	30.505635424280165	20.35898578047411	21.74433085414399	27.391047941101736
46	27.37988060993704	26.279302480966916	20.883662131768236	25.457154777327805
47	25.08276686314059	22.73380031180694	23.512972611806628	28.670460213245835
48	25.686963395621525	21.516906272840487	27.079805638247485	25.716324693290503
49	30.90000533271432	20.003576055485325	23.927857785919752	25.168560825880604
50	26.149027407014714	19.98484881754902	23.994265763660376	29.87185801177589
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.0
4	8.0
5	18.5
6	29.0
7	285.0
8	541.0
9	1388.0
10	2235.0
11	2563.0
12	2891.0
13	3439.5
14	3988.0
15	4045.5
16	4103.0
17	4862.5
18	5622.0
19	5975.5
20	6329.0
21	7617.0
22	8905.0
23	10903.5
24	12902.0
25	13623.0
26	14344.0
27	16842.5
28	19341.0
29	21703.0
30	24065.0
31	26681.5
32	29298.0
33	34465.5
34	39633.0
35	41981.0
36	44329.0
37	49930.0
38	55531.0
39	59600.5
40	63670.0
41	70005.0
42	76340.0
43	83546.5
44	90753.0
45	97486.0
46	104219.0
47	118926.5
48	133634.0
49	137434.0
50	141234.0
51	150386.0
52	159538.0
53	175598.5
54	191659.0
55	218132.0
56	244605.0
57	293679.0
58	342753.0
59	349327.0
60	355901.0
61	372164.0
62	388427.0
63	301286.0
64	214145.0
65	173928.5
66	133712.0
67	108869.5
68	84027.0
69	77152.0
70	70277.0
71	57017.5
72	43758.0
73	44902.0
74	46046.0
75	32359.0
76	18672.0
77	12496.5
78	6321.0
79	4886.0
80	3451.0
81	1970.5
82	490.0
83	296.5
84	103.0
85	64.5
86	26.0
87	19.0
88	12.0
89	6.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3187870.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.17531212274907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92029174747684	8.149070203599768
2	9.306230067498321	2.080000513808118
3	3.3222723300948727	1.113822907367491
4	1.5006641557434945	0.670815613274211
5	0.8204171513590325	0.4584208868646928
6	0.5492817355734788	0.3683036903015368
7	0.3877769648926648	0.30334700316814867
8	0.3442896880748508	0.30780357799043007
9	0.30030335525900387	0.30203853538753495
>10	6.834804421920318	21.046173224942084
>50	2.38301705830295	18.229394919009266
>100	1.230528131996853	26.641796441023725
>500	0.060073915084419574	4.569493867771559
>1k	0.03581871932263515	7.735719612235268
>5k	0.0028203716002074917	1.985699093403661
>10k+	0.0014101858001037458	6.038099909852535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	79750	2.5016703943385394	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	42281	1.3263087892542669	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	29255	0.9176973966943445	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	26601	0.8344443154833792	No Hit
AAAACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	13686	0.4293148716854828	No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	8633	0.2708077807438823	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	7743	0.24288945283214183	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC	7617	0.23893697045362575	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	6929	0.21735516191061743	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5955	0.18680184574653294	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	5893	0.18485697346504093	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	5097	0.1598873228833045	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5075	0.1591972069124525	No Hit
AAAACGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT	5033	0.1578797127862805	No Hit
AAAACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC	5026	0.15766013043191848	No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4963	0.15568388924266047	No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	4839	0.1517941446796764	No Hit
AAAACGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	4832	0.15157456232531438	No Hit
AAAACGGGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAG	4770	0.14962969004382237	No Hit
AAAACGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAG	4505	0.14131692948583224	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	4459	0.13987395972859623	No Hit
AAAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4374	0.13720760256848616	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	4369	0.13705075802965616	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	4044	0.126855863005706	No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	4028	0.12635396048145	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG	3944	0.12371897222910595	No Hit
AAAACGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG	3842	0.12051934363697389	No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	3726	0.11688055033611784	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	3591	0.11264574778770778	No Hit
AAAACGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3478	0.10910106121014972	No Hit
AAAACGGGGGGGGACTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTG	3446	0.1080972561616377	No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3402	0.10671702421993369	No Hit
AAAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	3378	0.10596417043354966	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	3328	0.10439572504524965	No Hit
AAAACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	3273	0.10267043511811962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.019025242560079E-4	0.0	0.0	0.0	0.0
8	0.0012547563106400199	0.0	0.0	0.0	0.0
9	0.004956287427028078	0.0	0.0	0.0	0.0
10	0.019448722814920306	0.0	0.0	0.0	0.0
11	0.036576146455156576	0.0	0.0	0.0	0.0
12	0.05502106422156487	0.0	0.0	0.0	0.0
13	0.06687851135711306	0.0	0.0	0.0	0.0
14	0.08143368456053729	0.0	0.0	0.0	0.0
15	0.09674171155034553	0.0	0.0	0.0	0.0
16	0.11079498222951376	0.0	0.0	0.0	0.0
17	0.12349938987474395	0.0	0.0	0.0	0.0
18	0.13403934288412012	0.0	0.0	0.0	0.0
19	0.15398996822329644	0.0	0.0	0.0	0.0
20	0.1713683431256607	0.0	0.0	0.0	0.0
21	0.18448054657184892	0.0	0.0	0.0	0.0
22	0.19850244834325115	0.0	0.0	0.0	0.0
23	0.21428100894954938	0.0	0.0	0.0	0.0
24	0.23009093846361364	0.0	0.0	0.0	0.0
25	0.2456499167155499	0.0	0.0	0.0	0.0
26	0.26243228237036015	0.0	0.0	0.0	0.0
27	0.27962244382612844	0.0	0.0	0.0	0.0
28	0.3016120481700948	0.0	0.0	0.0	0.0
29	0.316418172635647	0.0	0.0	0.0	0.0
30	0.33097334583907123	0.0	0.0	0.0	0.0
31	0.3421720459115334	0.0	0.0	0.0	0.0
32	0.3534648527072936	0.0	0.0	0.0	0.0
33	0.36350290319241374	0.0	0.0	0.0	0.0
34	0.37463886544934394	0.0	0.0	0.0	0.0
35	0.3930837832157522	0.0	0.0	0.0	0.0
36	0.4159517169771666	0.0	0.0	0.0	0.0
37	0.4347730616367669	0.0	0.0	0.0	0.0
38	0.4473519936509331	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAACGG	324625	0.0	43.000385	1
CGGGGAT	24900	0.0	42.992767	5
AAACGGG	322230	0.0	42.99091	2
AACGGGG	301400	0.0	42.986862	3
ACGGGGG	173495	0.0	42.95259	4
CGGGGGA	33735	0.0	42.80006	5
CGGGGGG	90890	0.0	42.782486	5
ACGGGGC	24695	0.0	42.619152	4
AACGGGT	6510	0.0	42.479263	3
ACGGGGA	91740	0.0	42.441246	4
ACGGGTA	2255	0.0	42.341465	4
CGGGGAG	33790	0.0	42.248592	5
CGGGGGT	23000	0.0	42.067825	5
ACGGGGT	13275	0.0	42.044445	4
CGGGGCA	8615	0.0	41.829365	5
ACGGGAT	2295	0.0	41.795208	4
AACGGGC	3905	0.0	41.74648	3
CGGGGGC	27725	0.0	41.555992	5
AACGGGA	11050	0.0	41.371944	3
ACGGGAG	4940	0.0	41.2834	4
>>END_MODULE