FastQCFastQC Report
Fri 17 Jun 2016
SRR1527455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671600
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC679232.5424090432699504No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT306701.1480011977840994No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA250340.9370414732744423No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC244760.9161551130408743No Hit
AAAACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC107770.4033912262314717No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC72250.27043719119628684No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT65860.246518939961072No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC62710.23472825273244496No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT61100.22870190148225783No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT56370.21099715526276389No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC50410.1886884264111394No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT46620.1745021709836802No Hit
AAAACGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA46200.1729300793531966No Hit
AAAACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT44950.16825123521485252No Hit
AAAACGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC42480.15900583919748465No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT42270.15821979338224285No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC41920.1569097170235065No Hit
AAAACGGGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGGC41830.15657284024554574No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC41320.15466387183710137No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT40560.15181913460098817No Hit
AAAACGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA39240.14687827519089683No Hit
AAAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC35820.13407695762838748No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC35390.13246743524479712No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC35380.13243000449169037No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC35030.13111992813295403No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT34910.130670759095673No Hit
AAAACGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA34550.12932325198382993No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT34230.12812546788441384No Hit
AAAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG31730.11876777960772571No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT30010.11232969007336428No Hit
AAAACGGGGGGGGACTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCT29120.10899835304686331No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC29020.10862404551579578No Hit
AAAACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA28790.10776313819434047No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT27990.10476867794580026No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC27960.10465638568648002No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT27350.10237310974696812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG206.3129625E-446.023
GCGATAA253.418379E-546.018
ATAACGC453.110472E-1046.020
AAAACGG2719150.044.9731331
ACGGGGG1476200.044.9685674
AACGGGG2526400.044.9503253
AAACGGG2700900.044.9440572
CGGGGGG780300.044.6765375
CGGGGAT212350.044.6461035
ACGGGGC201350.044.549294
CGGGGGA291800.044.518165
ACGGGTA18500.044.3837854
AACGGGT54450.044.3103753
ACGGGGA755500.044.0881584
CGGGGAG275650.043.888995
ACGGGGT111750.043.859514
CGGGGGT192200.043.822065
CGGGGTA27500.043.490915
ACGGGAT21100.043.383894
CGGGGGC234350.043.13425
Produced by FastQC (version 0.11.5)