##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2671600
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1643951190298	33.0	31.0	34.0	31.0	34.0
2	32.736155487348405	34.0	31.0	34.0	31.0	34.0
3	32.87141188800719	34.0	31.0	34.0	31.0	34.0
4	36.52803451115437	37.0	37.0	37.0	35.0	37.0
5	36.55398225782303	37.0	37.0	37.0	35.0	37.0
6	36.43890065878126	37.0	37.0	37.0	35.0	37.0
7	36.701003892798326	37.0	37.0	37.0	35.0	37.0
8	36.734192618655484	37.0	37.0	37.0	35.0	37.0
9	38.49390178170385	39.0	39.0	39.0	38.0	39.0
10	37.51247042970505	39.0	37.0	39.0	35.0	39.0
11	36.433390477616406	37.0	35.0	39.0	33.0	39.0
12	36.48934795628088	39.0	35.0	39.0	33.0	39.0
13	36.595797649348704	39.0	35.0	39.0	33.0	39.0
14	37.15288965413984	40.0	35.0	40.0	33.0	40.0
15	37.336751759245395	40.0	35.0	40.0	33.0	40.0
16	37.49771485252283	40.0	35.0	40.0	34.0	40.0
17	37.26931950890852	40.0	35.0	40.0	33.0	40.0
18	37.19493674202725	39.0	35.0	40.0	33.0	40.0
19	37.02763063332834	39.0	35.0	40.0	33.0	40.0
20	36.903852747417275	39.0	35.0	40.0	32.0	40.0
21	36.89577219643659	39.0	35.0	40.0	32.0	40.0
22	36.95838748315616	39.0	35.0	40.0	33.0	40.0
23	36.93758159904177	39.0	35.0	40.0	33.0	40.0
24	36.91801167839497	38.0	35.0	40.0	33.0	40.0
25	36.93439137595448	38.0	35.0	40.0	33.0	40.0
26	36.89183036382692	38.0	35.0	40.0	33.0	40.0
27	36.83148263213056	38.0	35.0	40.0	33.0	40.0
28	36.64730685731397	38.0	35.0	40.0	33.0	40.0
29	36.538883066327294	38.0	35.0	40.0	33.0	40.0
30	36.32932811798173	38.0	35.0	40.0	32.0	40.0
31	36.26965713430154	38.0	35.0	40.0	32.0	40.0
32	36.22430977691271	37.0	35.0	40.0	32.0	40.0
33	36.01487123820931	37.0	35.0	40.0	32.0	40.0
34	35.180202126066774	36.0	34.0	40.0	27.0	40.0
35	35.182072540799524	36.0	34.0	40.0	29.0	40.0
36	35.247431876029346	36.0	34.0	40.0	30.0	40.0
37	35.17617682287768	35.0	34.0	40.0	30.0	40.0
38	35.2270265009732	35.0	34.0	40.0	30.0	40.0
39	35.157368618056594	35.0	34.0	40.0	30.0	40.0
40	34.98249288815691	35.0	34.0	40.0	30.0	40.0
41	34.858970654289564	35.0	34.0	39.0	30.0	40.0
42	34.744472974996256	35.0	34.0	39.0	29.0	40.0
43	34.7480071867046	35.0	34.0	39.0	30.0	40.0
44	34.628235514298545	35.0	34.0	39.0	29.0	40.0
45	34.445365698457856	35.0	34.0	39.0	29.0	40.0
46	34.18762389579278	35.0	33.0	39.0	28.0	40.0
47	34.03377414283575	35.0	33.0	38.0	27.0	40.0
48	33.94653990118281	35.0	33.0	38.0	27.0	40.0
49	33.92600164695314	35.0	33.0	38.0	27.0	40.0
50	34.27707328941458	35.0	34.0	38.0	29.0	40.0
51	34.370084967809554	35.0	34.0	37.0	30.0	40.0
52	34.131004641413384	35.0	34.0	37.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	7.0
12	10.0
13	18.0
14	30.0
15	79.0
16	167.0
17	390.0
18	671.0
19	1161.0
20	1703.0
21	2776.0
22	4468.0
23	6486.0
24	8858.0
25	12532.0
26	17344.0
27	23354.0
28	29293.0
29	34581.0
30	44773.0
31	64319.0
32	94481.0
33	167092.0
34	444995.0
35	242307.0
36	285457.0
37	412613.0
38	638803.0
39	132830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.51564605479862	0.3609073214553077	0.0694340470130259	0.05401257673304387
8	99.202313220542	0.6099341218745321	0.11457553525976943	0.07317712232370116
9	93.51478514747717	3.259769426560862	1.9943853870339872	1.231060038927983
10	57.3813819434047	29.55648300643809	4.939848779757449	8.12228627039976
11	43.63516244946848	20.37501871537655	19.410241054050008	16.579577781104955
12	38.100801018116485	15.503331337026502	22.809215451414882	23.58665219344213
13	27.6136397664321	15.35465638568648	25.46533912262315	31.56636472525827
14	23.54297050456655	17.629809851774215	31.9955831711334	26.831636472525826
15	29.527811049558316	17.99988770774068	27.892162000299447	24.580139242401557
16	37.326545890103304	18.797312471926936	22.375168438388982	21.500973199580777
17	33.15238059589759	20.060488097020514	24.496968108998356	22.290163198083548
18	33.30588411438838	20.290462644108402	22.751197784099418	23.652455457403804
19	30.00142236861806	26.007336427608923	20.58298397963767	23.40825722413535
20	33.05644557568498	25.235252283275937	20.245695463392725	21.462606677646352
21	30.269651145381044	25.825684982781855	22.21264410839946	21.69201976343764
22	26.861992813295405	22.17255577182213	28.20721664919898	22.758234765683486
23	31.153540949243897	20.124719269351697	26.194714777661325	22.527025003743077
24	34.72256325797275	20.792371612516845	20.43165144482707	24.053413684683335
25	30.463617307980233	20.4850276987573	20.09990268004192	28.951452313220543
26	24.579839796376703	20.258983380745622	25.77582721964366	29.38534960323402
27	24.24363677197185	18.64452013774517	24.72731696361731	32.38452612666567
28	25.26130408743824	25.323139691570596	20.584368917502623	28.831187303488548
29	31.090058391974846	24.103196586315317	19.618880071867046	25.18786494984279
30	29.622997454708788	19.78552178469831	26.222600688725855	24.368880071867046
31	33.870527025003746	19.14014073963168	23.918962419523883	23.070369815840692
32	38.11236712082648	19.396803413684683	20.518939961072018	21.97188950441683
33	37.46185806258422	19.410278484803115	21.782864201227728	21.344999251384937
34	29.49292558766282	21.053787992214403	29.452986974097918	20.000299446024854
35	26.531741278634524	28.678956430603385	25.547013025902082	19.24228926486001
36	26.6391301092978	30.8220541997305	20.723199580775564	21.81561611019614
37	31.940672256325797	27.42921844587513	18.620527025003746	22.009582272795328
38	31.333133702650095	27.801092977990717	19.26980086839347	21.595972450965714
39	33.10383290911813	23.875879622698008	19.256550381793684	23.763737086390176
40	30.111431351998803	21.428058092528822	19.505876628237758	28.954633927234617
41	25.07332684533613	25.74655637071418	20.116521934421318	29.063594849528375
42	23.492064680341368	23.314380895343614	21.399236412636622	31.794318011678396
43	24.238134451265157	19.894482706992065	23.0061012127564	32.861281628986376
44	26.38115735888606	18.67206917203174	22.458788740829466	32.487984728252734
45	30.8104132355143	20.060862404551578	21.69639916155113	27.43232519838299
46	27.534249139092676	26.13793232519838	20.854618954933375	25.473199580775564
47	25.18692918101512	22.46429106153616	23.651519688576133	28.697260068872588
48	25.812434496182064	21.180341368468333	27.177908369516395	25.82931576583321
49	31.106003892798324	19.711483755053152	23.994310525527773	25.188201826620755
50	26.192431501721813	19.965638568648	23.954633927234617	29.88729600239557
51	23.209275340619854	21.41413385237311	27.345186405150475	28.031404401856562
52	23.42229375655038	20.968670459649648	25.59761940410241	30.011416379697557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	13.0
6	23.0
7	250.5
8	478.0
9	1115.5
10	1753.0
11	2216.0
12	2679.0
13	3041.5
14	3318.5
15	3233.0
16	3742.0
17	4251.0
18	4196.0
19	4141.0
20	4620.0
21	5099.0
22	5889.5
23	6680.0
24	7639.0
25	8598.0
26	10222.5
27	11847.0
28	14510.0
29	17173.0
30	19621.0
31	22069.0
32	25039.5
33	28010.0
34	29893.0
35	31776.0
36	36628.0
37	41480.0
38	43283.0
39	50778.0
40	56470.0
41	58408.5
42	60347.0
43	67836.0
44	75325.0
45	81714.5
46	88104.0
47	96239.0
48	104374.0
49	107152.0
50	109930.0
51	118776.0
52	127622.0
53	141269.0
54	154916.0
55	187985.5
56	221055.0
57	238706.0
58	256357.0
59	271670.0
60	286983.0
61	299585.0
62	312187.0
63	247693.5
64	153478.0
65	123756.0
66	107314.5
67	90873.0
68	74311.5
69	57750.0
70	51813.0
71	45876.0
72	43805.0
73	41734.0
74	32693.0
75	23652.0
76	15833.0
77	8014.0
78	5709.0
79	3404.0
80	2421.0
81	1438.0
82	852.5
83	267.0
84	196.5
85	126.0
86	83.0
87	40.0
88	25.0
89	7.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2671600.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.77425809796477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.68064542845146	6.7130235475248865
2	9.523115790498073	1.8616278326626392
3	3.4731129168026107	1.0184130615661189
4	1.6043096311915157	0.6272374561726618
5	0.9157376953667513	0.4475328292275033
6	0.6470681586341255	0.3794766713678852
7	0.5205975591959677	0.3561918436126317
8	0.44341471668546556	0.34672399082557326
9	0.39735044321954116	0.3495425208631642
>10	9.694579309343917	25.43663057151352
>50	2.618515169651404	17.444440999726
>100	1.3722077798697683	25.526107882644368
>500	0.0658382520644442	4.431030814813198
>1k	0.03927193982791408	7.695408668201408
>5k	0.0023101141075243577	1.387519586069461
>10k+	0.0019250950896036317	5.979091723209003
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	67923	2.5424090432699504	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	30670	1.1480011977840994	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	25034	0.9370414732744423	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	24476	0.9161551130408743	No Hit
AAAACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10777	0.4033912262314717	No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	7225	0.27043719119628684	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6586	0.246518939961072	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC	6271	0.23472825273244496	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	6110	0.22870190148225783	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	5637	0.21099715526276389	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	5041	0.1886884264111394	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4662	0.1745021709836802	No Hit
AAAACGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA	4620	0.1729300793531966	No Hit
AAAACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	4495	0.16825123521485252	No Hit
AAAACGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	4248	0.15900583919748465	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	4227	0.15821979338224285	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4192	0.1569097170235065	No Hit
AAAACGGGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGGC	4183	0.15657284024554574	No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	4132	0.15466387183710137	No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4056	0.15181913460098817	No Hit
AAAACGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA	3924	0.14687827519089683	No Hit
AAAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3582	0.13407695762838748	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3539	0.13246743524479712	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	3538	0.13243000449169037	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	3503	0.13111992813295403	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	3491	0.130670759095673	No Hit
AAAACGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA	3455	0.12932325198382993	No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3423	0.12812546788441384	No Hit
AAAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	3173	0.11876777960772571	No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3001	0.11232969007336428	No Hit
AAAACGGGGGGGGACTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCT	2912	0.10899835304686331	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	2902	0.10862404551579578	No Hit
AAAACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2879	0.10776313819434047	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	2799	0.10476867794580026	No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2796	0.10465638568648002	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	2735	0.10237310974696812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.7430753106752507E-4	0.0	0.0	0.0	0.0
8	0.0011229225932025753	0.0	0.0	0.0	0.0
9	0.004005090582422518	0.0	0.0	0.0	0.0
10	0.019613714627938313	0.0	0.0	0.0	0.0
11	0.039714029046264414	0.0	0.0	0.0	0.0
12	0.05936517442730948	0.0	0.0	0.0	0.0
13	0.07066926186554874	0.0	0.0	0.0	0.0
14	0.08717622398562659	0.0	0.0	0.0	0.0
15	0.1035708938463842	0.0	0.0	0.0	0.0
16	0.119478963916754	0.0	0.0	0.0	0.0
17	0.13411438838149423	0.0	0.0	0.0	0.0
18	0.14676598293157658	0.0	0.0	0.0	0.0
19	0.16855068123970654	0.0	0.0	0.0	0.0
20	0.1873409192992963	0.0	0.0	0.0	0.0
21	0.20171432849228926	0.0	0.0	0.0	0.0
22	0.21417876927683785	0.0	0.0	0.0	0.0
23	0.22780356340769575	0.0	0.0	0.0	0.0
24	0.24169037281030095	0.0	0.0	0.0	0.0
25	0.25351849079203476	0.0	0.0	0.0	0.0
26	0.26527174726755504	0.0	0.0	0.0	0.0
27	0.27777361880521034	0.0	0.0	0.0	0.0
28	0.294617457703249	0.0	0.0	0.0	0.0
29	0.30629585267255577	0.0	0.0	0.0	0.0
30	0.3179368168887558	0.0	0.0	0.0	0.0
31	0.3253481060038928	0.0	0.0	0.0	0.0
32	0.3334705794280581	0.0	0.0	0.0	0.0
33	0.34005839197484655	0.0	0.0	0.0	0.0
34	0.34952837251085495	0.0	0.0	0.0	0.0
35	0.365848180865399	0.0	0.0	0.0	0.0
36	0.38826920197634374	0.0	0.0	0.0	0.0
37	0.4051879023805959	0.0	0.0	0.0	0.0
38	0.4158182362629136	0.0	0.0	0.0	0.0
39	0.42468932474921395	0.0	0.0	0.0	0.0
40	0.43326096721066026	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	6.3129625E-4	46.0	23
GCGATAA	25	3.418379E-5	46.0	18
ATAACGC	45	3.110472E-10	46.0	20
AAAACGG	271915	0.0	44.973133	1
ACGGGGG	147620	0.0	44.968567	4
AACGGGG	252640	0.0	44.950325	3
AAACGGG	270090	0.0	44.944057	2
CGGGGGG	78030	0.0	44.676537	5
CGGGGAT	21235	0.0	44.646103	5
ACGGGGC	20135	0.0	44.54929	4
CGGGGGA	29180	0.0	44.51816	5
ACGGGTA	1850	0.0	44.383785	4
AACGGGT	5445	0.0	44.310375	3
ACGGGGA	75550	0.0	44.088158	4
CGGGGAG	27565	0.0	43.88899	5
ACGGGGT	11175	0.0	43.85951	4
CGGGGGT	19220	0.0	43.82206	5
CGGGGTA	2750	0.0	43.49091	5
ACGGGAT	2110	0.0	43.38389	4
CGGGGGC	23435	0.0	43.1342	5
>>END_MODULE