FastQCFastQC Report
Fri 17 Jun 2016
SRR1527454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3504002
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC858612.4503696059534215No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT390551.114582697155995No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC317160.9051364696709647No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA309190.8823910488635565No Hit
AAAACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC130140.37140389760051506No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC99040.28264824049757964No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT88330.25208318945023434No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT77140.22014827617107524No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC76470.21823617680583515No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT71330.20356723540682914No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC64090.1829051467436377No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT59530.16989145554140664No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC57600.16438346781765537No Hit
AAAACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT56250.16053073029067907No Hit
AAAACGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA56060.15998849315725278No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC55330.15790516101303595No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT54650.15596452285129975No Hit
AAAACGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC53300.15211178532432346No Hit
AAAACGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA51720.14760265547793638No Hit
AAAACGGGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGGC51630.14734580630947128No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT51300.1464040260250993No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC50060.14286521525958032No Hit
AAAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC47600.13584467132153463No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC45820.13076476554522515No Hit
AAAACGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA44800.12785380830262083No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT44100.1258560925478924No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC43880.12522823902497773No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT43770.1249143122635204No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT41510.11846454425539711No Hit
AAAACGGGGGGGGACTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCT38180.10896112502218891No Hit
AAAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG37910.10819057751679366No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT37860.10804788353431305No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC37800.10787665075533634No Hit
AAAACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA37730.10767687917986347No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC36580.1043949175828096No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT35800.10216889145611219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT283800.045.019385
AAAACGG3566200.044.999691
ACGGGGG1928200.044.9872934
AACGGGG3322400.044.982363
AAACGGG3544500.044.9734542
ACGGGTA24650.044.8803254
CGGGGGG1000800.044.7773785
ACGGGGC268450.044.586334
CGGGGGA385050.044.5186355
AACGGGT69250.044.3725623
ACGGGGA998450.044.299974
CGGGGAG353750.044.127495
CGGGGGT251550.043.9518975
ACGGGGT148000.043.948654
CGGGGGC317250.043.2885745
CGGGGCA89600.043.227685
AACGGGC41850.043.1971323
CGGGGAC180400.042.952885
ACGGGAT24000.042.9333344
AACGGGA118550.042.8376243
Produced by FastQC (version 0.11.5)