##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527454_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3504002
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.566547336445584	31.0	31.0	33.0	28.0	34.0
2	31.42230426809117	31.0	31.0	34.0	30.0	34.0
3	31.965621024188913	31.0	31.0	34.0	30.0	34.0
4	35.98300257819488	37.0	35.0	37.0	35.0	37.0
5	36.110978247158535	37.0	35.0	37.0	35.0	37.0
6	36.02332589992814	37.0	35.0	37.0	35.0	37.0
7	36.51384103091265	37.0	37.0	37.0	35.0	37.0
8	36.56763095454854	37.0	37.0	37.0	35.0	37.0
9	38.37366474105894	39.0	39.0	39.0	37.0	39.0
10	36.882057430332516	39.0	37.0	39.0	32.0	39.0
11	35.64661036152376	37.0	35.0	39.0	31.0	39.0
12	35.723202783560055	37.0	35.0	39.0	31.0	39.0
13	35.810124252212184	37.0	35.0	39.0	31.0	39.0
14	36.402312270369706	38.0	35.0	40.0	31.0	40.0
15	36.61701191951374	38.0	35.0	40.0	32.0	40.0
16	36.75594192012448	39.0	35.0	40.0	33.0	40.0
17	36.535549351855394	38.0	35.0	40.0	31.0	40.0
18	36.506101309302906	38.0	35.0	40.0	31.0	40.0
19	36.29870131352665	38.0	34.0	40.0	31.0	40.0
20	36.11605644060705	38.0	34.0	40.0	30.0	40.0
21	36.18512717743882	38.0	34.0	40.0	31.0	40.0
22	36.26324357120801	38.0	34.0	40.0	31.0	40.0
23	36.33163508468317	38.0	35.0	40.0	31.0	40.0
24	36.35580316449591	38.0	35.0	40.0	32.0	40.0
25	36.354548599001944	38.0	35.0	40.0	32.0	40.0
26	36.26757005275682	38.0	35.0	40.0	32.0	40.0
27	36.199842351688154	38.0	35.0	40.0	32.0	40.0
28	35.93666299277226	37.0	34.0	40.0	31.0	40.0
29	35.83664649734789	37.0	34.0	40.0	31.0	40.0
30	35.7081017647821	37.0	34.0	40.0	31.0	40.0
31	35.626946274574046	37.0	34.0	40.0	31.0	40.0
32	35.57513294798348	36.0	34.0	40.0	31.0	40.0
33	35.38280115136921	36.0	34.0	40.0	30.0	40.0
34	34.55145088387506	36.0	33.0	40.0	25.0	40.0
35	34.51353138497067	35.0	33.0	39.0	26.0	40.0
36	34.68531096728826	35.0	33.0	39.0	28.0	40.0
37	34.76097587843842	35.0	34.0	39.0	29.0	40.0
38	34.63842600546461	35.0	34.0	39.0	29.0	40.0
39	34.507504847314586	35.0	33.0	39.0	29.0	40.0
40	34.686954801966436	35.0	34.0	39.0	29.0	40.0
41	34.73593993382424	35.0	34.0	39.0	29.0	40.0
42	34.7724319221279	35.0	34.0	39.0	30.0	40.0
43	34.753391693269585	35.0	34.0	39.0	30.0	40.0
44	34.633615220539255	35.0	34.0	39.0	30.0	40.0
45	34.512565917485205	35.0	34.0	39.0	29.0	40.0
46	34.26602781619417	35.0	33.0	39.0	29.0	40.0
47	34.18184064963433	35.0	33.0	38.0	28.0	40.0
48	34.112021625558434	35.0	33.0	38.0	29.0	40.0
49	34.052648371776044	35.0	33.0	37.0	28.0	40.0
50	33.93913530871272	35.0	33.0	37.0	28.0	40.0
51	33.82367447278854	35.0	33.0	37.0	28.0	40.0
52	33.449331364536896	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	8.0
13	12.0
14	34.0
15	93.0
16	293.0
17	598.0
18	1249.0
19	2103.0
20	3588.0
21	5643.0
22	8563.0
23	12591.0
24	17515.0
25	24015.0
26	32297.0
27	42643.0
28	53451.0
29	65231.0
30	82397.0
31	111829.0
32	162039.0
33	266839.0
34	556103.0
35	307381.0
36	412538.0
37	548534.0
38	706692.0
39	79721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.55142719667397	0.34497697204510724	0.057534213736179375	0.04606161754473884
8	99.24834517788518	0.5904962383012339	0.09740291244126002	0.06375567137233369
9	93.56587125235659	3.2321328583716564	1.9738002432647013	1.2281956460070513
10	57.064921766597166	29.73385859939578	4.877736941930969	8.32348269207609
11	43.09378248071776	20.513915231783546	19.635890618783893	16.7564116687148
12	37.68419652728509	15.4182275010117	23.07407358785754	23.82350238384567
13	27.328380520330754	15.36848437871896	25.68143511333612	31.62169998761416
14	23.407378192135734	17.59405388467244	32.00098059304761	26.997587330144217
15	29.378379350240095	17.986205487325634	27.97992124433719	24.65549391809708
16	37.055914922422986	18.75369934149581	22.48063785351721	21.709747882563992
17	32.97769236433084	20.099018208322942	24.40323949586787	22.52004993147835
18	32.959113607811865	20.228812654787298	22.856550880964107	23.955522856436726
19	29.776467022564486	25.872245506709184	20.644394609363808	23.706892861362522
20	32.79221872590256	25.12273109433157	20.32581602407761	21.75923415568827
21	30.19370422733777	25.629665736492157	22.311859411039148	21.864770625130923
22	26.830806603420886	22.03083788194185	28.020446335361683	23.117909179275582
23	30.928035999979453	20.111175735630287	26.176469077357833	22.784319187032427
24	34.48548259961039	20.81985113022196	20.48229424526584	24.212372024901814
25	30.223555808472714	20.55329877094819	20.17227729892848	29.050868121650613
26	24.54456361611666	20.34273953039981	25.651554993404684	29.46114186007885
27	24.25983204347486	18.70795165071253	24.65908980645559	32.37312649935702
28	25.336543757680502	25.27173215083781	20.607693717069797	28.784030374411884
29	31.09139207112325	24.065111834981828	19.591940872179865	25.251555221715055
30	29.59692945380739	19.791170210519287	26.09090976546246	24.520990570210863
31	33.793901944119895	19.22792852287185	23.801613126933148	23.17655640607511
32	37.80388823978982	19.492340472408408	20.583778205606045	22.11999308219573
33	37.21011003989153	19.48098203140295	21.79687682826665	21.51203110043887
34	29.320388515759984	21.144365785179346	29.32175837799179	20.213487321068882
35	26.502410672140027	28.617049876113082	25.503895260333753	19.376644191413135
36	26.604122942852204	30.596386645898033	20.734177663140603	22.06531274810916
37	31.709913407583674	27.338568870679868	18.761975592479686	22.189542129256775
38	31.246899973230608	27.600555022514257	19.325046047348145	21.827498956906986
39	32.821014371567145	23.86285738421382	19.340200148287586	23.97592809593145
40	29.85472040255685	21.57104933159285	19.6306965578216	28.943533708028706
41	24.918165001047374	25.797844864243796	20.19025674072104	29.093733393987787
42	23.246876000641553	23.392423862771768	21.522961459496884	31.837738677089796
43	24.22709804389381	19.977300241266985	22.980152408588808	32.81544930625039
44	26.205350339411908	18.84682143446265	22.52196203084359	32.42586619528185
45	30.55666064117543	20.143652886042872	21.793537789076606	27.50614868370509
46	27.519618995651257	26.049328738967613	20.949588499093323	25.481463766287803
47	25.15686349494093	22.502641265615715	23.684204518148107	28.656290721295253
48	25.817451017436632	21.173532435198382	27.139368071136943	25.86964847622804
49	30.912282584313594	19.80954919546279	23.970163259039236	25.308004961184384
50	26.126811571454585	20.002043377829125	24.00677853494376	29.864366515772538
51	23.18429041992556	21.460290262391403	27.221588343842267	28.133830973840766
52	23.38120811574879	21.129753921373332	25.50717722193081	29.981860740947063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.5
4	9.0
5	18.0
6	27.0
7	309.5
8	592.0
9	1431.0
10	2270.0
11	2829.0
12	3388.0
13	3758.5
14	4148.5
15	4168.0
16	4882.0
17	5596.0
18	5665.0
19	5734.0
20	6477.5
21	7221.0
22	8303.5
23	9386.0
24	10676.5
25	11967.0
26	13957.0
27	15947.0
28	19365.5
29	22784.0
30	25671.0
31	28558.0
32	32375.5
33	36193.0
34	38579.5
35	40966.0
36	47367.0
37	53768.0
38	56602.0
39	66922.0
40	74408.0
41	76651.0
42	78894.0
43	88923.0
44	98952.0
45	107764.0
46	116576.0
47	127071.0
48	137566.0
49	142042.5
50	146519.0
51	157765.5
52	169012.0
53	187528.0
54	206044.0
55	247652.5
56	289261.0
57	312782.5
58	336304.0
59	354584.0
60	372864.0
61	387802.0
62	402740.0
63	320911.0
64	201741.0
65	164400.0
66	141518.0
67	118636.0
68	97629.5
69	76623.0
70	68152.0
71	59681.0
72	57722.0
73	55763.0
74	43576.5
75	31390.0
76	20939.5
77	10489.0
78	7429.5
79	4370.0
80	3102.0
81	1834.0
82	1054.5
83	275.0
84	189.5
85	104.0
86	69.5
87	35.0
88	27.5
89	17.5
90	15.0
91	10.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3504002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.60149759420523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.73125215161559	6.791259473688605
2	9.879217250914937	1.8971056133458115
3	3.670984506468955	1.0574084672167903
4	1.7832716550085737	0.6848831402151678
5	0.9291297510544423	0.44605185347268655
6	0.5942444692976856	0.34233821053988955
7	0.41577145583271985	0.2794420043041946
8	0.33320278294954664	0.25593965750980474
9	0.30041920395342736	0.2596026837610695
>10	6.736227702079788	18.1131711366876
>50	2.9468666448353824	19.550880707544476
>100	1.547635346033276	28.705539693906907
>500	0.08258427861477775	5.246237745107897
>1k	0.04263376116214159	7.554902961144084
>5k	0.0050683492290657835	3.0739168864676234
>10k+	0.0014906909497252303	5.741319765087387
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	85861	2.4503696059534215	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	39055	1.114582697155995	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	31716	0.9051364696709647	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	30919	0.8823910488635565	No Hit
AAAACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	13014	0.37140389760051506	No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	9904	0.28264824049757964	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	8833	0.25208318945023434	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	7714	0.22014827617107524	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC	7647	0.21823617680583515	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	7133	0.20356723540682914	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	6409	0.1829051467436377	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5953	0.16989145554140664	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	5760	0.16438346781765537	No Hit
AAAACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	5625	0.16053073029067907	No Hit
AAAACGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA	5606	0.15998849315725278	No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5533	0.15790516101303595	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	5465	0.15596452285129975	No Hit
AAAACGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	5330	0.15211178532432346	No Hit
AAAACGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA	5172	0.14760265547793638	No Hit
AAAACGGGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGGC	5163	0.14734580630947128	No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5130	0.1464040260250993	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5006	0.14286521525958032	No Hit
AAAACGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4760	0.13584467132153463	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	4582	0.13076476554522515	No Hit
AAAACGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA	4480	0.12785380830262083	No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4410	0.1258560925478924	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	4388	0.12522823902497773	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	4377	0.1249143122635204	No Hit
AAAACGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	4151	0.11846454425539711	No Hit
AAAACGGGGGGGGACTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCT	3818	0.10896112502218891	No Hit
AAAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	3791	0.10819057751679366	No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	3786	0.10804788353431305	No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3780	0.10787665075533634	No Hit
AAAACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3773	0.10767687917986347	No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	3658	0.1043949175828096	No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	3580	0.10216889145611219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.4246555795344866E-4	0.0	0.0	0.0	0.0
8	9.417802843719838E-4	0.0	0.0	0.0	0.0
9	0.004594746235875436	0.0	0.0	0.0	0.0
10	0.01783674781007545	0.0	0.0	0.0	0.0
11	0.034731715335778915	0.0	0.0	0.0	0.0
12	0.05042805340864531	0.0	0.0	0.0	0.0
13	0.06015978301382248	0.0	0.0	0.0	0.0
14	0.0738298665354643	0.0	0.0	0.0	0.0
15	0.08807072598702854	0.0	0.0	0.0	0.0
16	0.1006848740383139	0.0	0.0	0.0	0.0
17	0.11244285819471564	0.0	0.0	0.0	0.0
18	0.12374422160717945	0.0	0.0	0.0	0.0
19	0.14123850385930145	0.0	0.0	0.0	0.0
20	0.15550790210736182	0.0	0.0	0.0	0.0
21	0.16723734746726743	0.0	0.0	0.0	0.0
22	0.17842455569374674	0.0	0.0	0.0	0.0
23	0.1903252338326291	0.0	0.0	0.0	0.0
24	0.20351015781383686	0.0	0.0	0.0	0.0
25	0.21643823262657955	0.0	0.0	0.0	0.0
26	0.2289096866953843	0.0	0.0	0.0	0.0
27	0.24072474844477829	0.0	0.0	0.0	0.0
28	0.25787656513894686	0.0	0.0	0.0	0.0
29	0.26952039410936407	0.0	0.0	0.0	0.0
30	0.28076467992883564	0.0	0.0	0.0	0.0
31	0.28869846535475724	0.0	0.0	0.0	0.0
32	0.2970317939316245	0.0	0.0	0.0	0.0
33	0.30362425592222836	0.0	0.0	0.0	0.0
34	0.3126425156150025	0.0	0.0	0.0	0.0
35	0.32982287110566716	0.0	0.0	0.0	0.0
36	0.34902948114755644	0.0	0.0	0.0	0.0
37	0.36424065967998875	0.0	0.0	0.0	0.0
38	0.37454316521508835	0.0	0.0	0.0	0.0
39	0.3833331145358935	0.0	0.0	0.0	0.0
40	0.3925226070076444	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGAT	28380	0.0	45.01938	5
AAAACGG	356620	0.0	44.99969	1
ACGGGGG	192820	0.0	44.987293	4
AACGGGG	332240	0.0	44.98236	3
AAACGGG	354450	0.0	44.973454	2
ACGGGTA	2465	0.0	44.880325	4
CGGGGGG	100080	0.0	44.777378	5
ACGGGGC	26845	0.0	44.58633	4
CGGGGGA	38505	0.0	44.518635	5
AACGGGT	6925	0.0	44.372562	3
ACGGGGA	99845	0.0	44.29997	4
CGGGGAG	35375	0.0	44.12749	5
CGGGGGT	25155	0.0	43.951897	5
ACGGGGT	14800	0.0	43.94865	4
CGGGGGC	31725	0.0	43.288574	5
CGGGGCA	8960	0.0	43.22768	5
AACGGGC	4185	0.0	43.197132	3
CGGGGAC	18040	0.0	42.95288	5
ACGGGAT	2400	0.0	42.933334	4
AACGGGA	11855	0.0	42.837624	3
>>END_MODULE