FastQCFastQC Report
Fri 17 Jun 2016
SRR1527453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327048
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC767402.3065492292266296No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG440541.324116754552384No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT140250.4215448649974392No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC104490.314062195676167No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG85240.2562030965588714No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTG76920.2311959430702533No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC71200.2140035250468283No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT66080.198614507515371No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC60090.18061055927056058No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT58940.17715404166095589No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG53570.1610136072578454No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC38140.11463615793941054No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT37870.11382462771802511No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGT37430.11250213402391548No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTG35530.10679136579935125No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA33300.10008872730420482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG3431100.042.4643441
CGAATGG3419900.042.4618842
GAATGGG3393000.042.3524323
AATGGGA977250.042.327354
AATGGGG1835200.042.2545784
AATGGGC374100.042.1475524
ATGGGGG842800.042.1414345
ATGGGAT284350.041.8878145
ATGGGAG313200.041.822485
TGGGGGG529300.041.7887766
ATGGGGC318000.041.7031445
ATGGGGA423400.041.4227685
CGAATAG13150.041.1558952
ATGGGGT273350.041.0784725
ATGGGAC150450.040.9438325
AATGGGT221750.040.696284
ATGGGAA251200.040.5843935
ATGGGCA199550.040.5602575
GCGAATA14000.040.542861
CGAATGA10050.040.497512