FastQCFastQC Report
Fri 17 Jun 2016
SRR1527452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855702
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC665022.328744385793756No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT368491.2903657314383643No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC118470.4148542109785965No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA102700.35963136209590496No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT76100.26648438807690716No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCT68120.23854029587120784No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC60020.21017599175264084No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC57260.2005111177566847No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT53010.18562861250928844No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC50140.17557854425987024No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT46340.1622718336857277No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC33890.11867484772570808No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC33640.11779940624056713No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC32160.11261679264853265No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT31770.11125110393171278No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCT30770.10774933799114893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG351.02028935E-745.99999637
GCGAATG2946900.044.328991
CGAATGG2938200.044.3107342
GAATGGG2914200.044.1894873
AATGGGA836950.044.114824
AATGGGC318100.044.0839354
AATGGGG1576800.044.0599944
ATGGGGG718150.043.9086535
CGAATAG12400.043.7741932
ATGGGAG268750.043.749215
ATGGGGC274200.043.575865
ATGGGAT242850.043.4807475
TGGGGGG455600.043.2991646
ATGGGAC130650.042.9896665
ATGGGGT233800.042.9110375
ATGGGGA372650.042.8275835
ATGGGCA166850.042.8019185
ATGGGCT91900.042.696415
AATGGGT196800.042.4705284
CGAATGA8200.042.353662