##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2855702
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7641693706136	33.0	31.0	33.0	30.0	34.0
2	32.23105141923072	34.0	31.0	34.0	31.0	34.0
3	32.811055215145	34.0	31.0	34.0	31.0	34.0
4	34.96076866563808	35.0	35.0	37.0	33.0	37.0
5	35.58791568588039	37.0	35.0	37.0	35.0	37.0
6	35.95278183788084	37.0	35.0	37.0	35.0	37.0
7	36.483820790824815	37.0	37.0	37.0	35.0	37.0
8	36.64823815650232	37.0	37.0	37.0	35.0	37.0
9	38.717604638018955	39.0	39.0	39.0	39.0	39.0
10	37.55055709594348	39.0	37.0	39.0	34.0	39.0
11	37.648424100273765	39.0	37.0	39.0	35.0	39.0
12	37.23685909804314	39.0	35.0	39.0	35.0	39.0
13	37.198587597725535	39.0	37.0	39.0	35.0	39.0
14	37.86338035271187	40.0	37.0	40.0	34.0	40.0
15	37.92475720505851	40.0	37.0	40.0	35.0	40.0
16	38.049611969316125	40.0	37.0	40.0	35.0	40.0
17	37.86858887937187	40.0	37.0	40.0	34.0	40.0
18	37.720298896733624	40.0	36.0	40.0	33.0	40.0
19	37.66150634765112	40.0	36.0	40.0	33.0	40.0
20	37.54786213687563	40.0	36.0	40.0	33.0	40.0
21	37.523693648707045	40.0	36.0	40.0	33.0	40.0
22	37.56690719129657	40.0	36.0	40.0	33.0	40.0
23	37.53749130686605	39.0	36.0	40.0	34.0	40.0
24	37.58095382501396	39.0	36.0	40.0	34.0	40.0
25	37.60900647196381	39.0	36.0	40.0	34.0	40.0
26	37.54759355142799	39.0	35.0	40.0	34.0	40.0
27	37.53604717859216	39.0	35.0	40.0	34.0	40.0
28	37.401919037770746	39.0	35.0	40.0	34.0	40.0
29	37.33671475525107	39.0	35.0	40.0	34.0	40.0
30	37.192187420115964	39.0	35.0	40.0	33.0	40.0
31	37.14602083830876	39.0	35.0	40.0	33.0	40.0
32	37.10917350619918	39.0	35.0	40.0	33.0	40.0
33	37.00444584203814	39.0	35.0	40.0	33.0	40.0
34	36.45535213408122	39.0	35.0	40.0	31.0	40.0
35	36.36187564388721	38.0	35.0	40.0	31.0	40.0
36	36.505392369371876	38.0	35.0	40.0	31.0	40.0
37	36.5521588036847	38.0	35.0	40.0	32.0	40.0
38	36.700181601581676	39.0	35.0	40.0	32.0	40.0
39	36.696911302369784	39.0	35.0	40.0	32.0	40.0
40	36.60210098952902	39.0	35.0	40.0	32.0	40.0
41	36.48840495261761	38.0	35.0	40.0	32.0	40.0
42	36.39526288107093	38.0	35.0	40.0	31.0	40.0
43	36.3983987124707	38.0	35.0	40.0	31.0	40.0
44	36.308286718992385	38.0	35.0	40.0	31.0	40.0
45	36.215847101693385	38.0	35.0	40.0	31.0	40.0
46	35.97348918059377	38.0	35.0	40.0	31.0	40.0
47	35.85041261308078	38.0	35.0	40.0	30.0	40.0
48	35.76564011230864	38.0	35.0	40.0	30.0	40.0
49	35.68157321737352	38.0	35.0	40.0	30.0	40.0
50	36.09410540735693	38.0	35.0	40.0	31.0	40.0
51	36.22446739890927	38.0	35.0	40.0	32.0	40.0
52	35.934454995654306	37.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	7.0
12	29.0
13	47.0
14	72.0
15	107.0
16	239.0
17	400.0
18	696.0
19	1023.0
20	1436.0
21	2245.0
22	3240.0
23	4607.0
24	6385.0
25	8839.0
26	12063.0
27	15776.0
28	21403.0
29	27878.0
30	37146.0
31	51371.0
32	72727.0
33	118709.0
34	289408.0
35	184613.0
36	244574.0
37	459329.0
38	1079039.0
39	212288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.49038800266975	0.41709534118055736	0.06912485966672993	0.02339179648296636
8	99.60156907128265	0.2900162551974961	0.077248956648838	0.031165716871018056
9	98.81104541020036	0.5770209916861073	0.19974072924976066	0.41219286886376805
10	53.93276329252842	28.52275902737751	6.400877962756618	11.143599717337453
11	37.59772553298629	26.774747505166857	19.668894023255927	15.958632938590931
12	34.644476209352376	19.13757107709418	23.938422146288374	22.27953056726507
13	26.81764413793876	18.561425526893213	26.254700245333723	28.366230089834303
14	22.82195411145841	20.213593715310633	30.329565199730226	26.634886973500738
15	26.765502843083766	20.62729234352884	29.11788414897633	23.48932066441106
16	33.79662863982306	21.737562252644008	23.765399891165114	20.700409216367817
17	30.853254296141547	22.59017222385249	25.43829853395067	21.118274946055298
18	29.570242273178366	22.722749082362235	25.64119085254694	22.065817791912462
19	28.868068166776506	26.952742267925718	22.43956127074884	21.73962829454894
20	28.623224692212286	27.394595094306055	22.801013551133835	21.181166662347824
21	27.91723366093521	28.157314733820265	23.566709691697525	20.358741913547
22	26.679359400945895	24.758185552974364	27.658838352181004	20.903616693898734
23	28.430837671437708	23.494363207365474	26.41273494223137	21.66206417896545
24	31.626724357093284	22.59370900745246	23.35607847037261	22.42348816508165
25	28.444704664562337	22.702859051819832	22.227424290069482	26.62501199354835
26	23.54576913137295	23.412982166906772	26.79950499036664	26.24174371135364
27	22.726215830643394	21.677717072719773	26.692876217476474	28.903190879160363
28	23.846570825667385	26.784132237887565	22.773419635522192	26.595877300922854
29	28.424709581041718	26.52486148764822	22.188239529194572	22.862189402115487
30	28.26243774735599	22.738191870160122	25.47244075187117	23.526929630612717
31	30.47765488135667	22.30309745204507	25.147792031521497	22.07145563507677
32	33.17226377262053	22.893810348558777	23.075762106830474	20.858163771990217
33	33.253609795419834	22.34522369631005	23.259849942325914	21.141316565944205
34	28.09505333539704	23.40727428842365	28.682194430651375	19.815477945527928
35	23.75072749187415	29.016262901381168	27.420648232903854	19.81236137384083
36	23.678976307752002	32.57038024275642	23.42796972513238	20.322673724359195
37	28.829023476539216	29.150625660520603	21.4654750390622	20.554875823877982
38	27.197165530577074	29.573358844865467	22.098384215159705	21.131091409397758
39	29.4630182000783	27.136269820870666	21.477135919644276	21.923576059406756
40	27.737978262437746	24.62574876510224	21.742814901554855	25.893458070905158
41	23.364622779267584	28.132907425214537	22.635695181079818	25.86677461443806
42	22.51677520973827	26.30554588679071	22.74078317695614	28.436895726514884
43	22.589962117896057	23.74316367744253	23.241640759434983	30.42523344522643
44	23.13375135080621	23.010068977785497	23.47972582573392	30.376453845674373
45	27.406886292757438	23.806020376075654	22.632823733008557	26.154269598158354
46	25.683457167449546	28.01027558197599	22.424363606566793	23.881903644007675
47	22.495449455160237	27.19846118397508	24.068512750980318	26.237576609884368
48	24.06129911314276	24.120689063494723	27.428036959038444	24.389974864324078
49	27.752650661728712	23.041654906569384	25.310098882866626	23.895595548835278
50	24.681181719941367	23.822933905568576	24.393896842177508	27.101987532312542
51	22.64620047890151	23.669066310140206	27.46133875313321	26.223394457825083
52	22.52311340609069	23.366618785853706	26.924027787213088	27.18624002084251
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	25.0
6	50.0
7	35.0
8	20.0
9	34.5
10	49.0
11	847.0
12	1645.0
13	3826.0
14	7205.0
15	8403.0
16	7909.0
17	7415.0
18	7149.5
19	6884.0
20	6991.5
21	7099.0
22	7732.0
23	8365.0
24	9110.0
25	9855.0
26	12750.0
27	15645.0
28	16966.0
29	18287.0
30	22772.0
31	27257.0
32	31279.0
33	35301.0
34	39557.5
35	43814.0
36	50401.0
37	56988.0
38	63542.0
39	77058.0
40	84020.0
41	86924.0
42	89828.0
43	96823.5
44	103819.0
45	110892.5
46	117966.0
47	123120.5
48	128275.0
49	129717.0
50	131159.0
51	144330.0
52	157501.0
53	165467.5
54	173434.0
55	177704.0
56	181974.0
57	194800.5
58	207627.0
59	214132.0
60	220637.0
61	233683.0
62	246729.0
63	267740.0
64	218478.0
65	148205.0
66	121405.0
67	94605.0
68	74152.5
69	53700.0
70	44941.5
71	36183.0
72	30890.0
73	25597.0
74	21998.5
75	18400.0
76	14896.5
77	11393.0
78	9170.0
79	6947.0
80	5313.0
81	3679.0
82	2569.0
83	1459.0
84	926.0
85	393.0
86	287.0
87	181.0
88	113.5
89	26.5
90	7.0
91	6.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2855702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.382940710722803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.85462090691374	6.17910003496698
2	9.229234045402444	1.73194711706791
3	3.3666361444525617	0.9476684201392438
4	1.6255502479243626	0.6100976639430019
5	0.9706002545797867	0.45535423212672993
6	0.7355483376615544	0.41409638652894487
7	0.5843675224429216	0.3838160071447737
8	0.5300116364482577	0.3978454208629737
9	0.4817363413477307	0.406809317615965
>10	12.165348379596635	29.374431539288864
>50	2.772173322331331	17.781969548704605
>100	1.6004522750819525	27.88999303719026
>500	0.054061723576716694	3.4338544914889884
>1k	0.02590457588051008	4.288725037236371
>5k	0.0022525718156965286	1.284525072139372
>10k+	0.0015017145437976858	4.4197666735549905
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	66502	2.328744385793756	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	36849	1.2903657314383643	No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	11847	0.4148542109785965	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10270	0.35963136209590496	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	7610	0.26648438807690716	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCT	6812	0.23854029587120784	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	6002	0.21017599175264084	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	5726	0.2005111177566847	No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5301	0.18562861250928844	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	5014	0.17557854425987024	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	4634	0.1622718336857277	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC	3389	0.11867484772570808	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC	3364	0.11779940624056713	No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	3216	0.11261679264853265	No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	3177	0.11125110393171278	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCT	3077	0.10774933799114893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.00504254295441191	0.0	0.0	0.0	0.0
8	0.0053577018890626545	0.0	0.0	0.0	0.0
9	0.006233143374203611	0.0	0.0	0.0	0.0
10	0.01897957139785594	0.0	0.0	0.0	0.0
11	0.0390797078966923	0.0	0.0	0.0	0.0
12	0.055538007817342284	0.0	0.0	0.0	0.0
13	0.06429242266875185	0.0	0.0	0.0	0.0
14	0.0723815019914543	0.0	0.0	0.0	0.0
15	0.08117093450226949	0.0	0.0	0.0	0.0
16	0.09139609104871586	0.0	0.0	0.0	0.0
17	0.09941513505260703	0.0	0.0	0.0	0.0
18	0.10690891416541362	0.0	0.0	0.0	0.0
19	0.11895498900095318	0.0	0.0	0.0	0.0
20	0.13012562235135178	0.0	0.0	0.0	0.0
21	0.13824971933345986	0.0	0.0	0.0	0.0
22	0.1447630039829086	0.0	0.0	0.0	0.0
23	0.15208169479868697	0.0	0.0	0.0	0.0
24	0.1596104915708992	0.0	0.0	0.0	0.0
25	0.16734939429954526	0.0	0.0	0.0	0.0
26	0.17505327936878567	0.0	0.0	0.0	0.0
27	0.18223189954694152	0.0	0.0	0.0	0.0
28	0.1937527094913965	0.0	0.0	0.0	0.0
29	0.20156164753885383	0.0	0.0	0.0	0.0
30	0.20961570920215064	0.0	0.0	0.0	0.0
31	0.21756471788723053	0.0	0.0	0.0	0.0
32	0.22498846168122585	0.0	0.0	0.0	0.0
33	0.23195697590294787	0.0	0.0	0.0	0.0
34	0.24064135543554616	0.0	0.0	0.0	0.0
35	0.25356287175622666	0.0	0.0	0.0	0.0
36	0.2718420899659698	0.0	0.0	0.0	0.0
37	0.2861993303222815	0.0	0.0	0.0	0.0
38	0.2961093279340772	0.0	0.0	0.0	0.0
39	0.30468865448845855	0.0	0.0	0.0	0.0
40	0.3133380163616512	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	35	1.02028935E-7	45.999996	37
GCGAATG	294690	0.0	44.32899	1
CGAATGG	293820	0.0	44.310734	2
GAATGGG	291420	0.0	44.189487	3
AATGGGA	83695	0.0	44.11482	4
AATGGGC	31810	0.0	44.083935	4
AATGGGG	157680	0.0	44.059994	4
ATGGGGG	71815	0.0	43.908653	5
CGAATAG	1240	0.0	43.774193	2
ATGGGAG	26875	0.0	43.74921	5
ATGGGGC	27420	0.0	43.57586	5
ATGGGAT	24285	0.0	43.480747	5
TGGGGGG	45560	0.0	43.299164	6
ATGGGAC	13065	0.0	42.989666	5
ATGGGGT	23380	0.0	42.911037	5
ATGGGGA	37265	0.0	42.827583	5
ATGGGCA	16685	0.0	42.801918	5
ATGGGCT	9190	0.0	42.69641	5
AATGGGT	19680	0.0	42.470528	4
CGAATGA	820	0.0	42.35366	2
>>END_MODULE