FastQCFastQC Report
Fri 17 Jun 2016
SRR1527451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103586
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC696522.2442426277216097No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT384781.2397916474684447No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC120550.38842165159915015No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA101440.3268477174468502No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT79030.25464092182398035No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCT74150.23891717516447103No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC63010.20302321250321403No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC62850.20250767982585308No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC55160.1777298905201918No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT52570.16938470530541122No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT50340.1621994686146928No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT36460.11747700885362933No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC35980.11593041082154643No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTC33930.10932514839285909No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC33520.10800409590712164No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCT33300.1072952384757503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA405.6170393E-946.014
ACGATAG750.046.037
GCGAATG3197450.044.4124531
CGAATGG3186800.044.4049842
GAATGGG3159850.044.2880173
AATGGGA902500.044.2186164
AATGGGG1709050.044.2181934
ATGGGGG778650.044.1686255
AATGGGC355150.044.070114
ATGGGAT266050.043.795535
ATGGGGC296300.043.7100945
TGGGGGG490450.043.5895586
ATGGGAG282800.043.551985
CGAATAG13550.043.284132
ATGGGGA398900.043.0363545
ATGGGGT258250.042.829435
ATGGGAC146250.042.7917945
ATGGGCG45050.042.7835735
ATGGGCA186150.042.6392675
AATGGGT206950.042.5880664