FastQCFastQC Report
Fri 17 Jun 2016
SRR1527450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140737
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGA27411.9476043968537058No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG10850.7709415434462863Illumina Single End Adapter 2 (100% over 23bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG7410.5265139941877403No Hit
GTCCCGGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA7370.5236718133824083Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC6820.4845918273090942No Hit
GTCCCGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT5680.4035896743571342No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGC5490.39008931553180753No Hit
GTCCCGGGGTCGCTTGGGGATGGGAGTTTTGTAGACTGTACAGAAATTGG5120.3637991430824872No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC4450.3161926145931773No Hit
GTCCCGGGGCAAGTTCAGTGGGAAACCCTGGGTTTATCCCCCAGCTTAAG4310.3062449817745156No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA4130.2934551681505219No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGCCTGGGA3890.27640208331853033No Hit
GTCCCGGGGAGTCTCTAAGCAAACAAGAGGCGCGAGGGAGCCTCAGCTAA3830.2721388121105324No Hit
GTCCCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA3690.26219117929187064Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC3610.25650681768120676Illumina Single End Adapter 2 (95% over 22bp)
GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA3210.22808500962788747Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGGCTGTGCGGCGCTTGCGCTCCACTCGGCCTTCTTAAAGGA3110.22097955761455768No Hit
GTCCCGGGGACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA3070.21813737680922574Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGAGGCTGCACCGATTTACAGTGCTTGAGATTTTGGTGATGA2930.20818974399056397No Hit
GTCCCGGGGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT2720.19326829476257132No Hit
GTCCCGGGGATGCTGTTGGAAACGTATTCATCGCTTGATGGTGAAAAACT2720.19326829476257132No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC2700.19184720435990535Illumina Single End Adapter 2 (100% over 21bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACCCGGGGA2660.18900502355457344No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA2660.18900502355457344No Hit
GTCCCGGGGATCAAACTGTAAGCCCAGCTCTTAGCCTGTCCTCTACCTCA2620.1861628427492415No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC2620.1861628427492415No Hit
GTCCCGGGGGATCAAATCATCCGTAGCATTCGTCGAAAGTGTTACTAGTA2600.18474175234657553No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCCGGGA2580.18332066194390956No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA2540.18047848113857762No Hit
GTCCCGGGGGTACTGGGGGCTCTGCTACGCGTCAAACGCCTGGAGAACCC2540.18047848113857762No Hit
GTCCCGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC2420.17195193872258183No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCGCGGGGA2360.16768866751458394No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAAGAGCTCG2360.16768866751458394No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCCGCGTATGCCG2300.16342539630658603No Hit
GTCCCGGGGGAAAAAAGATGGCAGAGCGCGGTTACAGCTTCTCGCTGACT2290.16271485110525305No Hit
GTCCCGGGGGACTCCATCTTAGAGAACCTAAGGTTGAACTGGAGTTAACA2290.16271485110525305No Hit
GTCCCGGGGTAAACACAAAGTGTATAAATCTTGAGTATATATTTACATGT2210.1570304894945892No Hit
GTCCCGGGGGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGGCCGGAG2200.1563199442932562No Hit
GTCCCGGGGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTC2170.1541883086892573No Hit
GTCCCGGGGGAGAAATCACTCTTGGAAGCCGCTATCCCAGAACTTCAAAA2150.15276721828659132No Hit
GTCCCGGGGAAGGAAAGAGAATGGGCCAGAGCACACCAAGTCTGGGGCTT2120.15063558268259236No Hit
GTCCCGGGGATACAATCACGGTCTGAGCTCCCTGCCCTGAAGAGGCTGTG2110.14992503748125938No Hit
GTCCCGGGGGGGCCAAAGGAATACCAGTGTCACTCTTTATGTCTTGGCTA2100.1492144922799264No Hit
GTCCCGGGGACCCGAGCAGCTGCGCCACGGGACTAGAGAGCGTGGAAAGA2090.1485039470785934No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG2060.14637231147459445No Hit
GTCCCGGGGTTATCTTTTGGGGACAAAATCAAACATTAGATCCTAAAAAC2050.14566176627326147No Hit
GTCCCGGGGGGAGGATCAGGCCTTGGAGATGCCGAGCTGGAAAGCTCCGG2040.14495122107192848No Hit
GTCCCGGGGAACTAAACATGGGCAAAGGAGATCCTAAAAAGCCGAGAGGC2000.14210904026659654No Hit
GTCCCGGGGATAATGGTAGAATATTATTATTCCTACTTTTCTACATATGT1980.1406879498639306No Hit
GTCCCGGGGGTATAAAACGTAAAAAGTTTACTGATTGGGGTGGGACAATA1950.13855631425993165No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT1940.13784576905859866No Hit
GTCCCGGGGGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGAT1910.1357141334545997No Hit
GTCCCGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC1890.13429304305193374No Hit
GTCCCGTGGGGCCGAAGGCCTCGGAGAAAGCGCCGACAAGGCCCCGCCTG1880.13358249785060075No Hit
GTCCCGGGGGAGTCTGTTGTGACATAGGAACATGCTTCCAGTTGCTTGAA1860.1321614074479348No Hit
GTCCCGGGGGCACCTGCTTTTAGGACATCACTTAACTTTGTGGAAACAGA1820.12931922664260287No Hit
GTCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGAAG1810.12860868144126988No Hit
GTCCCGGGGAGTGCTGTCCCGGGGATACCAGGCAGTCACTTCCGGTCCTG1810.12860868144126988No Hit
GTCCCGGGGAGGACAGATATAATCTCTGGAAAGCCATTGGTACTGACTGT1760.125055955434605No Hit
GTCCCGGGGGACCTGTGACTGCCAGTCTCTAGGCTGTAGAACACAAACCT1750.12434541023327199No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCACGGGGA1740.123634865031939No Hit
GTCCCGGGGATAAATGAATGAAATATAGATAAACTGTGAGATAAATATCA1720.12221377462927303No Hit
GTCCCGGGGGTCCTGTAGCAGCACAAACCAGGTGGAACAAAAACTTCCAT1720.12221377462927303No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGT1720.12221377462927303No Hit
GTCCCGGGGAGTGAGCTTGGTTTTAAACAGCAGAGTATTTAGGCAGTGCC1720.12221377462927303No Hit
GTCCCGGGGGAAATGGAGATTGACAGTATGTGAAGGCTTAGAGGCAAATG1710.12150322942794005No Hit
GTCCCGGGGGGGGTTCTGTGGCTCATCATTCAAATGGTTCATGACATTTT1710.12150322942794005No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC1680.1193715938239411No Hit
GTCCCGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA1680.1193715938239411No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAGCGCTCG1650.11723995821994217No Hit
GTCCCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA1610.11439777741461023No Hit
GTCCCGGGGAGTGGGCGGTAGGCGGCGCTGCCGGTGGCTGGTTGCTGTCC1600.11368723221327724No Hit
GTCCCGGGGGTCGCACTGATTATGTTCAACTTGAAATCGTTAGCAATATG1570.11155559660927832No Hit
GTCCCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA1560.11084505140794533No Hit
GTCCCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1560.11084505140794533Illumina Single End Adapter 2 (97% over 34bp)
GTCCCGGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA1500.10658178019994742Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGTAATGCCTGAATATTTTGTGCATGGAATAATGTAATAGGA1490.10587123499861444No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCG1490.10587123499861444No Hit
GTCCCGGGGAGCATCCAGTTTGTAGACTGGTGCCCCACTGGCTTCAAGGT1470.10445014459594848No Hit
GTCCCGGGGGCATTACTTATATGACATGTCTCCATACCCATTACAATCTC1470.10445014459594848No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGT1460.1037395993946155Illumina Single End Adapter 2 (95% over 24bp)
GTCCCGGGGCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA1460.1037395993946155No Hit
GTCCCGGGGGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTA1440.10231850899194952No Hit
GTCCCGGGGTGTGCTTTTGGTGAAAAAACAAATTGATGAAAGTCAATTTC1440.10231850899194952No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTAT1420.10089741858928356No Hit
GTCCCGGGGGTGCAGTGCTGTCCCGGGGCAATGGAGGCGAGAATGACGCT1420.10089741858928356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG408.223651E-944.00000433
CTATGCC408.223651E-944.00000439
AGCCGAG207.832918E-444.00000440
TCGTTAG408.223651E-944.00000437
GTCGCAT207.832918E-444.00000441
TCGCACT408.223651E-944.00000411
CAAACGC207.832918E-444.00000433
GATAATT207.832918E-444.00000435
GATAATG408.223651E-944.0000049
TTAATGG207.832918E-444.00000422
AGTTTCC207.832918E-444.00000417
ATTAGAT408.223651E-944.00000435
ATTAGAC207.832918E-444.00000438
CTCAACG207.832918E-444.00000433
TACCAGG207.832918E-444.00000426
ATAGGAA207.832918E-444.00000424
CGAAAGT408.223651E-944.00000434
GTTTCAC207.832918E-444.00000426
TGCCACA207.832918E-444.00000411
TTAGCAA408.223651E-944.00000440
Produced by FastQC (version 0.11.5)