FastQCFastQC Report
Fri 17 Jun 2016
SRR1527449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119950
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG28292.358482701125469No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC9310.7761567319716549Illumina Single End Adapter 2 (100% over 25bp)
GTCCCGGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA8420.7019591496456857Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA6650.5543976656940391No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGGAG6490.5410587744893706No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA6280.523551479783243No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAG6140.511879949979158No Hit
GTCCCGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGT6130.5110462692788662No Hit
GTCCCGGGGTCGCTTGGGGATGGGAGTTTTGTAGACTGTACAGAAATTGGCA5720.47686536056690293No Hit
GTCCCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4510.3759899958315965Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG4150.3459774906210921No Hit
GTCCCGGGGACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA4140.3451438099208003Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC4040.33680700291788246No Hit
GTCCCGGGGCAAGTTCAGTGGGAAACCCTGGGTTTATCCCCCAGCTTAAGAA3780.315131304710296No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT3770.31429762401000416Illumina Single End Adapter 2 (95% over 24bp)
GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA3730.310962901208837Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG3410.2842851187994998Illumina Single End Adapter 2 (100% over 23bp)
GTCCCGGGGAGTCTCTAAGCAAACAAGAGGCGCGAGGGAGCCTCAGCTAAGC3320.2767819924968737No Hit
GTCCCGGGGGAGGCTGCACCGATTTACAGTGCTTGAGATTTTGGTGATGAGT3210.267611504793664No Hit
GTCCCGGGGGGCTGTGCGGCGCTTGCGCTCCACTCGGCCTTCTTAAAGGACT3170.2642767819924969No Hit
GTCCCGGGGGTACTGGGGGCTCTGCTACGCGTCAAACGCCTGGAGAACCCCT2740.22842851187994997No Hit
GTCCCGGGGGAAAAAAGATGGCAGAGCGCGGTTACAGCTTCTCGCTGACTAC2490.20758649437265528No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCCGGGACA2430.20258441017090453No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC2330.19424760316798667No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTCCGGGGAAG2320.19341392246769487No Hit
GTCCCGGGGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCG2320.19341392246769487No Hit
GTCCCGGGGATCAAACTGTAAGCCCAGCTCTTAGCCTGTCCTCTACCTCACA2290.1909128803668195No Hit
GTCCCGGGGGATCAAATCATCCGTAGCATTCGTCGAAAGTGTTACTAGTACC2250.18757815756565235No Hit
GTCCCGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA2230.18591079616506878No Hit
GTCCCGGGGATGCTGTTGGAAACGTATTCATCGCTTGATGGTGAAAAACTAT2210.18424343476448518No Hit
GTCCCGGGGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA2140.1784076698624427No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC2140.1784076698624427No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT2110.17590662776156732No Hit
GTCCCGGGGGACTCCATCTTAGAGAACCTAAGGTTGAACTGGAGTTAACAGG2090.17423926636098375No Hit
GTCCCGGGGATACAATCACGGTCTGAGCTCCCTGCCCTGAAGAGGCTGTGGG2060.17173822426010837No Hit
GTCCCGGGGTTATCTTTTGGGGACAAAATCAAACATTAGATCCTAAAAACAT2040.1700708628595248No Hit
GTCCCGGGGTAAACACAAAGTGTATAAATCTTGAGTATATATTTACATGTCT2030.16923718215923303No Hit
GTCCCGGGGGCACCTGCTTTTAGGACATCACTTAACTTTGTGGAAACAGAAA2020.16840350145894123No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACA1990.16590245935806586No Hit
GTCCCGGGGGAGAAATCACTCTTGGAAGCCGCTATCCCAGAACTTCAAAAGA1990.16590245935806586No Hit
GTCCCGGGGAACTAAACATGGGCAAAGGAGATCCTAAAAAGCCGAGAGGCAA1960.16340141725719048No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT1930.1609003751563151No Hit
GTCCCGGGGGTCCTGTAGCAGCACAAACCAGGTGGAACAAAAACTTCCATTT1920.16006669445602334No Hit
GTCCCGGGGGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGGCCGGAGCG1920.16006669445602334No Hit
GTCCCGGGGAAGGAAAGAGAATGGGCCAGAGCACACCAAGTCTGGGGCTTAT1920.16006669445602334No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGCCCGGGGAAG1910.15923301375573154No Hit
GTCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGAAGCA1890.157565652355148No Hit
GTCCCGTGGGGCCGAAGGCCTCGGAGAAAGCGCCGACAAGGCCCCGCCTGAG1880.1567319716548562No Hit
GTCCCGGGGGACCTGTGACTGCCAGTCTCTAGGCTGTAGAACACAAACCTCC1880.1567319716548562No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG1860.15506461025427262No Hit
GTCCCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1830.15256356815339725Illumina Single End Adapter 2 (97% over 34bp)
GTCCCGGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1830.15256356815339725Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTG1750.14589412255106293No Hit
GTCCCGGGGGAGTCTGTTGTGACATAGGAACATGCTTCCAGTTGCTTGAAAG1750.14589412255106293No Hit
GTCCCGGGGGTATAAAACGTAAAAAGTTTACTGATTGGGGTGGGACAATATT1750.14589412255106293No Hit
GTCCCGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT1750.14589412255106293No Hit
GTCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1730.1442267611504794Illumina Single End Adapter 2 (97% over 34bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCTGGGAAG1710.1425593997498958No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACCCGGGGAAG1700.14172571904960402No Hit
GTCCCGGGGAGTGAGCTTGGTTTTAAACAGCAGAGTATTTAGGCAGTGCCCG1700.14172571904960402No Hit
GTCCCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1690.14089203834931222Illumina Single End Adapter 2 (97% over 34bp)
GTCCCGGGGACCCGAGCAGCTGCGCCACGGGACTAGAGAGCGTGGAAAGAAA1680.14005835764902042No Hit
GTCCCGGGGATAATGGTAGAATATTATTATTCCTACTTTTCTACATATGTTG1660.13839099624843684No Hit
GTCCCGGGGATCTCCAGAGTGGACAGGCTGGAACACGACCTACCGCTTTTGG1640.13672363484785327No Hit
GTCCCGGGGGGAGGATCAGGCCTTGGAGATGCCGAGCTGGAAAGCTCCGGAG1610.1342225927469779No Hit
GTCCCGGGGGGGGTTCTGTGGCTCATCATTCAAATGGTTCATGACATTTTTT1590.13255523134639435No Hit
GTCCCGGGGCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG1590.13255523134639435No Hit
GTCCCGGGGAGCATCCAGTTTGTAGACTGGTGCCCCACTGGCTTCAAGGTTG1550.12922050854522718No Hit
GTCCCGGGGGGGCCAAAGGAATACCAGTGTCACTCTTTATGTCTTGGCTACA1550.12922050854522718No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT1520.1267194664443518No Hit
GTCCCGGGGATAAATGAATGAAATATAGATAAACTGTGAGATAAATATCATT1520.1267194664443518No Hit
GTCCCGGGGAGGACAGATATAATCTCTGGAAAGCCATTGGTACTGACTGTGG1510.12588578574406004No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT1510.12588578574406004Illumina Single End Adapter 2 (96% over 26bp)
GTCCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG1500.12505210504376824No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC1480.12338474364318465Illumina Single End Adapter 2 (95% over 21bp)
GTCCCGGGGGAAATGGAGATTGACAGTATGTGAAGGCTTAGAGGCAAATGCA1440.12005002084201752No Hit
GTCCCGGGGAGTGGGCGGTAGGCGGCGCTGCCGGTGGCTGGTTGCTGTCCAA1440.12005002084201752No Hit
GTCCCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG1420.11838265944143393No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1420.11838265944143393No Hit
GTCCCGGGGAGTGCTGTCCCGGGGATACCAGGCAGTCACTTCCGGTCCTGAC1400.11671529804085036No Hit
GTCCCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA1380.11504793664026677No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCCGGCCCGGGGCGG1360.1133805752396832No Hit
GTCCCGGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA1350.11254689453939141Illumina Single End Adapter 2 (97% over 34bp)
GTCCCGGGGGTCGCACTGATTATGTTCAACTTGAAATCGTTAGCAATATGCT1350.11254689453939141No Hit
GTCCCGGGGTGTGCTTTTGGTGAAAAAACAAATTGATGAAAGTCAATTTCAG1340.11171321383909961No Hit
GTCCCGGGGGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGA1330.11087953313880783No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA1310.10921217173822426No Hit
GTCCCGGGGATCTCAAAGCCATGTTCAGCAGCAGCGTTTGGACAGCAGTGTT1300.10837849103793246No Hit
GTCCCGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG1260.10504376823676533No Hit
GTCCCGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG1250.10421008753647354No Hit
GTCCCGGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT1250.10421008753647354Illumina Single End Adapter 2 (100% over 29bp)
GTCCCGGGGGCATTACTTATATGACATGTCTCCATACCCATTACAATCTCCA1240.10337640683618174No Hit
GTCCCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG1210.10087536473530638No Hit
GTCCCGGGGGTTCGGATGTTAAGAAAATGTCATCAACACTCGACTCCGCCAA1210.10087536473530638No Hit
GTCCCGGGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1210.10087536473530638Illumina Single End Adapter 2 (97% over 34bp)
GTCCCGGGGGGCTGGGCCTGGCTCCAGGGCAGGCCCATGTACTACTTTGACT1210.10087536473530638No Hit
GTCCCGGGGGGTAGCAATTTTTTATTCGTGGAATTCTCGTCCAAAATTTCTA1200.1000416840350146No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAA253.3987595E-546.020
TATTTAC453.0559022E-1046.040
GCCCCCA206.288762E-446.039
ACAACAA253.3987595E-546.023
TATCAGA253.3987595E-546.031
TATCAAG253.3987595E-546.019
CTATGGT253.3987595E-546.036
TTAGGAC206.288762E-446.020
GGAATAC253.3987595E-546.018
GCCCATC206.288762E-446.024
GATACAA405.5388227E-946.09
CAAACCT301.8473984E-646.044
GTTCTTA453.0559022E-1046.020
CCGGGAT1650.046.04
CAAACAA453.0559022E-1046.020
AATCATC253.3987595E-546.015
GCGACAG253.3987595E-546.022
GATGTCC206.288762E-446.09
AGTTTAC453.0559022E-1046.025
CTGTACT253.3987595E-546.015
Produced by FastQC (version 0.11.5)