FastQCFastQC Report
Fri 17 Jun 2016
SRR1527448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124626
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG22291.788551345626113No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC9770.7839455651308715Illumina Single End Adapter 2 (100% over 25bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA7310.5865549724776532No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA6740.5408181278384928No Hit
GTCCCGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGT6260.5023028902476209No Hit
GTCCCGGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6090.4886620769341871Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAG5710.4581708471747469No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGGAG5640.4525540416927447No Hit
GTCCCGGGGTCGCTTGGGGATGGGAGTTTTGTAGACTGTACAGAAATTGGCA5170.41484120488501597No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC4780.3835475743424326No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG4480.3594755508481376No Hit
GTCCCGGGGCAAGTTCAGTGGGAAACCCTGGGTTTATCCCCCAGCTTAAGAA4470.3586731500649945No Hit
GTCCCGGGGAGTCTCTAAGCAAACAAGAGGCGCGAGGGAGCCTCAGCTAAGC3750.3009002936786866No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG3080.2471394412080946Illumina Single End Adapter 2 (100% over 23bp)
GTCCCGGGGGGCTGTGCGGCGCTTGCGCTCCACTCGGCCTTCTTAAAGGACT3020.24232503650923562No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT2980.23911543337666297Illumina Single End Adapter 2 (95% over 24bp)
GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA2750.22066021536437022Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGAGGCTGCACCGATTTACAGTGCTTGAGATTTTGGTGATGAGT2750.22066021536437022No Hit
GTCCCGGGGATGCTGTTGGAAACGTATTCATCGCTTGATGGTGAAAAACTAT2730.21905541379808385No Hit
GTCCCGGGGGATCAAATCATCCGTAGCATTCGTCGAAAGTGTTACTAGTACC2720.2182530130149407No Hit
GTCCCGGGGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA2620.21022900518350907No Hit
GTCCCGGGGGAAAAAAGATGGCAGAGCGCGGTTACAGCTTCTCGCTGACTAC2600.20862420361722275No Hit
GTCCCGGGGATCAAACTGTAAGCCCAGCTCTTAGCCTGTCCTCTACCTCACA2590.20782180283407956No Hit
GTCCCGGGGACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2530.2030073981352206Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGAAGGAAAGAGAATGGGCCAGAGCACACCAAGTCTGGGGCTTAT2500.20060019578579108No Hit
GTCCCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2490.1997977950026479Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGTACTGGGGGCTCTGCTACGCGTCAAACGCCTGGAGAACCCCT2310.18535458090607096No Hit
GTCCCGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA2310.18535458090607096No Hit
GTCCCGGGGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCG2280.18294737855664148No Hit
GTCCCGGGGGCACCTGCTTTTAGGACATCACTTAACTTTGTGGAAACAGAAA2210.17733057307463934No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT2180.17492337072520983No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACA2150.17251616837578035No Hit
GTCCCGGGGATACAATCACGGTCTGAGCTCCCTGCCCTGAAGAGGCTGTGGG2150.17251616837578035No Hit
GTCCCGGGGGTATAAAACGTAAAAAGTTTACTGATTGGGGTGGGACAATATT2140.17171376759263715No Hit
GTCCCGGGGAACTAAACATGGGCAAAGGAGATCCTAAAAAGCCGAGAGGCAA2060.16529456132749185No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTCCGGGGAAG2050.1644921605443487No Hit
GTCCCGGGGGGGCCAAAGGAATACCAGTGTCACTCTTTATGTCTTGGCTACA2040.16368975976120553No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC2030.16288735897806236No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC2000.16048015662863288No Hit
GTCCCGGGGGAGTCTGTTGTGACATAGGAACATGCTTCCAGTTGCTTGAAAG2000.16048015662863288No Hit
GTCCCGGGGGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGGCCGGAGCG1980.15887535506234654No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT1980.15887535506234654No Hit
GTCCCGGGGTAAACACAAAGTGTATAAATCTTGAGTATATATTTACATGTCT1970.15807295427920337No Hit
GTCCCGGGGGACTCCATCTTAGAGAACCTAAGGTTGAACTGGAGTTAACAGG1960.1572705534960602No Hit
GTCCCGGGGGACCTGTGACTGCCAGTCTCTAGGCTGTAGAACACAAACCTCC1910.15325854958034438No Hit
GTCCCGGGGGGAGGATCAGGCCTTGGAGATGCCGAGCTGGAAAGCTCCGGAG1910.15325854958034438No Hit
GTCCCGGGGGTCCTGTAGCAGCACAAACCAGGTGGAACAAAAACTTCCATTT1900.1524561487972012No Hit
GTCCCGTGGGGCCGAAGGCCTCGGAGAAAGCGCCGACAAGGCCCCGCCTGAG1890.15165374801405807No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCGTC1870.15004894644777172No Hit
GTCCCGGGGACCCGAGCAGCTGCGCCACGGGACTAGAGAGCGTGGAAAGAAA1850.14844414488148538No Hit
GTCCCGGGGTTATCTTTTGGGGACAAAATCAAACATTAGATCCTAAAAACAT1830.14683934331519907No Hit
GTCCCGGGGGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTG1810.14523454174891276No Hit
GTCCCGGGGGGGGTTCTGTGGCTCATCATTCAAATGGTTCATGACATTTTTT1800.1444321409657696No Hit
GTCCCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG1750.14042013705005377No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG1740.1396177362669106No Hit
GTCCCGGGGATAATGGTAGAATATTATTATTCCTACTTTTCTACATATGTTG1710.13721053391748111No Hit
GTCCCGGGGAGTGCTGTCCCGGGGATACCAGGCAGTCACTTCCGGTCCTGAC1700.13640813313433794No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCCGGGACA1690.13560573235119477No Hit
GTCCCGGGGGAAATGGAGATTGACAGTATGTGAAGGCTTAGAGGCAAATGCA1690.13560573235119477No Hit
GTCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGAAGCA1680.1348033315680516No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTGCCTGGGAAG1660.1331985300017653No Hit
GTCCCGGGGGTCGCACTGATTATGTTCAACTTGAAATCGTTAGCAATATGCT1650.13239612921862212No Hit
GTCCCGGGGAGTGAGCTTGGTTTTAAACAGCAGAGTATTTAGGCAGTGCCCG1620.1299889268691926No Hit
GTCCCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA1570.1259769229534768No Hit
GTCCCGGGGCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG1560.12517452217033362No Hit
GTCCCGGGGAGGACAGATATAATCTCTGGAAAGCCATTGGTACTGACTGTGG1530.12276731982090415No Hit
GTCCCGGGGAGCATCCAGTTTGTAGACTGGTGCCCCACTGGCTTCAAGGTTG1530.12276731982090415No Hit
GTCCCGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG1510.12116251825461781No Hit
GTCCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG1510.12116251825461781No Hit
GTCCCGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT1480.11875531590518833No Hit
GTCCCGGGGATAAATGAATGAAATATAGATAAACTGTGAGATAAATATCATT1470.11795291512204517No Hit
GTCCCGGGGGGCTGGGCCTGGCTCCAGGGCAGGCCCATGTACTACTTTGACT1460.117150514338902No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT1440.11554571277261566No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTCGGAAGAGCTCGTA1430.1147433119894725No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCGTA1430.1147433119894725No Hit
GTCCCGGGGATCTCCAGAGTGGACAGGCTGGAACACGACCTACCGCTTTTGG1400.11233610964004302No Hit
GTCCCGGGGTGTGCTTTTGGTGAAAAAACAAATTGATGAAAGTCAATTTCAG1400.11233610964004302No Hit
GTCCCGGGGGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGA1390.11153370885689984No Hit
GTCCCGGGGGCATTACTTATATGACATGTCTCCATACCCATTACAATCTCCA1380.11073130807375668No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACCCGGGGAAG1380.11073130807375668No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCTGGGAAG1360.10912650650747036No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1350.1083241057243272No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC1320.10591690337489769Illumina Single End Adapter 2 (95% over 21bp)
GTCCCGGGGTGGCTGGATGGCAAGCATGTGGTCTTTGGGAAGGTGAAAGAAG1310.10511450259175453No Hit
GTCCCGGGGGGTAGCAATTTTTTATTCGTGGAATTCTCGTCCAAAATTTCTA1310.10511450259175453No Hit
GTCCCGGGGGTGCAGTGCTGTCCCGGGGCAATGGAGGCGAGAATGACGCTGT1300.10431210180861138No Hit
GTCCCGGGTGGAGAAACCGAAGTGTAGGTGACGGTTCCGAGACATCGCCGCC1290.1035097010254682No Hit
GTCCCGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG1270.10190489945918187No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA1250.10030009789289554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA351.0097756E-746.00000435
CAAACGC351.0097756E-746.00000433
TCCGCTA700.046.00000438
CTACTAC351.0097756E-746.00000440
CGACGAC351.0097756E-746.00000415
ACTTGAC351.0097756E-746.00000441
TCACAAC351.0097756E-746.00000446
ATTCGAA351.0097756E-746.00000424
CATTCGA351.0097756E-746.00000423
CCGCTAA700.046.00000439
GACCCAT351.0097756E-746.00000419
CCGTCGC351.0097756E-746.00000434
TAGTTGG351.0097756E-746.00000425
CGTTAAA351.0097756E-746.00000431
AAACGCC351.0097756E-746.00000434
TACGCGT351.0097756E-746.00000426
AGTGACG700.046.00000410
CTACGCG351.0097756E-746.00000425
CCTTTGT351.0097756E-746.00000418
TACTGGG351.0097756E-746.00000411
Produced by FastQC (version 0.11.5)