FastQCFastQC Report
Fri 17 Jun 2016
SRR1527447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338107
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT133420.9970802036010574No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC86680.6477807828521934No Hit
TATGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG52930.395558800604137No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC51980.3884592188815992No Hit
TATGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC44790.33472659510786507No Hit
TATGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC43410.32441351850038896No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA41630.3111111443255285No Hit
TATGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA38270.2860010447594998No Hit
TATGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG32440.2424320327148726No Hit
TATGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT30110.2250193743848586No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAA29590.22113328754725892No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG28240.21104440825733664No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG27280.20387009409561416No Hit
TATGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA26580.1986388233526915No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA25620.19146450919096902No Hit
TATGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG24350.18197348941452365No Hit
TATGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG23770.1776390079418163No Hit
TATGTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC21900.16366404181429436No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA21370.15970322253751007No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG20270.15148265422720306No Hit
TATGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA19910.14879228641655712No Hit
TATGTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT19770.1477460322679726No Hit
TATGTAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA19370.14475673470058822No Hit
TATGTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA18420.13765715297805034No Hit
TATGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA17790.13294900930941994No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA17740.1325753471134969No Hit
TATGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTC17200.128539795397528No Hit
TATGTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA16730.12502737075585135No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA16640.12435477880318989No Hit
TATGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT15850.11845091610760575No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT15790.1180025214724981No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA15530.11605947805369825No Hit
TATGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT15420.11523742122266756No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT14970.11187446145936014No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT14970.11187446145936014No Hit
TATGTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC14620.10925882608789879No Hit
TATGTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC14320.10701685291236052No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC14080.1052232743719299No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAA13770.10290656875720701No Hit
TATGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC13500.10088879289922256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC207.856945E-444.09
AACCGTA207.856945E-444.023
AGCGTAC207.856945E-444.011
ACTATCG207.856945E-444.022
CGTATTT207.856945E-444.017
ATCTATA351.4463149E-744.036
GGTAAAT254.4432323E-544.08
CCAATTG207.856945E-444.030
TATATCG302.5280297E-644.020
TCGATAC254.4432323E-544.025
CATTAGG302.5280297E-644.033
TAGTTCA302.5280297E-644.013
CTAAATG207.856945E-444.021
TACGACA351.4463149E-744.038
CGAATAT302.5280297E-644.041
GATGCAT254.4432323E-544.09
CGTACCC207.856945E-444.043
GTTCAAC254.4432323E-544.013
TTAGTGG302.5280297E-644.018
CATAACA207.856945E-444.010
Produced by FastQC (version 0.11.5)