##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1338107
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298625595710956	31.0	31.0	33.0	30.0	34.0
2	29.9119405249356	31.0	30.0	33.0	25.0	33.0
3	31.41971456692178	31.0	31.0	33.0	30.0	34.0
4	35.378473470357754	35.0	35.0	37.0	35.0	37.0
5	35.31130245936984	35.0	35.0	37.0	33.0	37.0
6	35.51578386481798	37.0	35.0	37.0	33.0	37.0
7	36.15547486112845	37.0	35.0	37.0	35.0	37.0
8	36.582848755742255	37.0	37.0	37.0	35.0	37.0
9	38.65213843138105	39.0	39.0	39.0	39.0	39.0
10	37.51381914899182	39.0	37.0	39.0	35.0	39.0
11	36.98091931362739	39.0	37.0	39.0	34.0	39.0
12	36.89543586574168	38.0	35.0	39.0	34.0	39.0
13	36.889452786660556	39.0	37.0	39.0	33.0	39.0
14	37.76237699974666	40.0	37.0	40.0	33.0	40.0
15	37.807564716424025	40.0	37.0	40.0	33.0	40.0
16	37.96063767695708	40.0	38.0	40.0	34.0	40.0
17	37.88024649747741	40.0	37.0	40.0	34.0	40.0
18	37.736444843349595	40.0	37.0	40.0	33.0	40.0
19	37.66829259543519	39.0	37.0	40.0	34.0	40.0
20	37.51218325589807	39.0	37.0	40.0	33.0	40.0
21	37.408968789491425	39.0	36.0	40.0	33.0	40.0
22	37.21295830602485	39.0	36.0	40.0	33.0	40.0
23	36.97040819605607	38.0	36.0	40.0	32.0	40.0
24	36.80908701621021	38.0	35.0	40.0	32.0	40.0
25	36.51712307012817	38.0	35.0	40.0	32.0	40.0
26	35.779815814430386	38.0	35.0	39.0	30.0	40.0
27	35.62570930426341	37.0	34.0	39.0	30.0	40.0
28	35.40764527799347	37.0	34.0	39.0	30.0	40.0
29	35.095849584525006	37.0	34.0	38.0	30.0	40.0
30	34.755185497123925	36.0	33.0	38.0	29.0	40.0
31	34.58034820832714	36.0	33.0	38.0	29.0	40.0
32	34.492109375408695	36.0	33.0	38.0	28.0	40.0
33	33.910643169791356	36.0	33.0	38.0	26.0	39.0
34	33.36623678076566	35.0	32.0	38.0	25.0	39.0
35	33.17171496748765	35.0	31.0	38.0	24.0	39.0
36	32.7233472360581	35.0	31.0	38.0	23.0	39.0
37	32.40021911551169	34.0	31.0	37.0	23.0	39.0
38	31.783612969665356	34.0	30.0	37.0	21.0	39.0
39	30.821340146938923	33.0	29.0	36.0	18.0	38.0
40	30.473086980338643	33.0	28.0	36.0	18.0	38.0
41	30.16237490723836	33.0	28.0	36.0	17.0	38.0
42	29.791191586323066	32.0	27.0	35.0	17.0	38.0
43	29.302544564821797	32.0	27.0	35.0	15.0	38.0
44	28.86740223315475	31.0	26.0	34.0	15.0	37.0
45	28.372030039451253	31.0	25.0	34.0	15.0	36.0
46	28.18819048103029	31.0	25.0	34.0	15.0	36.0
47	27.67795026855102	30.0	24.0	33.0	13.0	36.0
48	27.092095774104763	29.0	23.0	33.0	12.0	35.0
49	26.2934062821583	29.0	23.0	32.0	10.0	35.0
50	29.100735591398895	32.0	26.0	34.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	13.0
14	23.0
15	73.0
16	173.0
17	323.0
18	660.0
19	1196.0
20	1911.0
21	3075.0
22	4784.0
23	7044.0
24	10067.0
25	14097.0
26	19311.0
27	26343.0
28	34790.0
29	41706.0
30	47589.0
31	59463.0
32	85573.0
33	120940.0
34	172641.0
35	300779.0
36	309958.0
37	73861.0
38	1706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.45804035103322	0.4590066414718703	0.06972536575924047	0.013227641735675847
8	98.94754305896315	0.9255612593013862	0.08526971310963921	0.04162596862582738
9	98.42987145273135	0.8512024823126999	0.33749169535769563	0.3814343695982459
10	70.35169833204668	19.22678829122036	3.7165936655289897	6.704919711203962
11	34.49993161981815	32.31639921172223	14.572078316606968	18.611590851852654
12	31.763603359073677	23.04591486331063	24.11899795756244	21.071483820053256
13	23.398651976261988	22.24425998817733	28.14677749985614	26.210310535704544
14	25.074452192537667	22.66462995859076	27.06226034240909	25.198657506462485
15	24.638463142334658	22.281700940208818	25.947551279531456	27.132284637925068
16	28.553172504142044	22.171844254607443	23.664101600245722	25.61088164100479
17	30.325751229161796	22.63922092926799	20.694832326562825	26.340195515007398
18	29.552569413357826	25.074526924976855	20.30031978010727	25.072583881558053
19	30.759274108871715	23.147177318405777	19.85364399110086	26.239904581621648
20	28.505642672820635	24.378095324215476	20.581313751441403	26.53494825152249
21	31.869648690276637	23.045690665993078	22.27609600726997	22.808564636460314
22	31.234497689646645	22.674419908123937	21.714182797040895	24.376899605188523
23	30.03989964928066	25.598251858782596	19.78556273900368	24.57628575293306
24	28.65024994264285	22.450670985205218	23.683307837116168	25.21577123503576
25	29.595316368571424	23.00742765713056	21.445594410611406	25.951661563686613
26	28.71863012449677	22.049806181418973	22.94517553528978	26.286388158794477
27	30.121731670187813	22.50843916069492	21.163105790493585	26.206723378623682
28	32.386722436994944	23.47114244227106	18.98517831533652	25.156956805397474
29	32.13173535449706	24.75863290454351	18.205644242201856	24.903987498757573
30	32.10176764638403	23.192390444112466	18.590516304002595	26.115325605500907
31	33.00625435783536	24.611335266910643	17.734829875338818	24.64758049991518
32	34.3821532956632	24.810123555141704	18.186736935088152	22.620986214106946
33	33.306902960675046	26.28429565049731	18.158712270393924	22.250089118433728
34	30.20311529645985	28.208805424379367	19.819790196150233	21.76828908301055
35	30.71189374242867	29.010011904877565	20.123203899239748	20.154890453454023
36	32.07284619241959	28.693893687126664	17.059995949501797	22.17326417095195
37	31.260878240678807	29.134665613437488	17.402793648041598	22.2016624978421
38	33.522057653087536	27.63583181315097	17.44075772714738	21.401352806614117
39	31.988323803701796	27.408196803394645	16.817788114104477	23.785691278799078
40	30.685812121153237	26.953524643395482	18.887054622687124	23.473608612764153
41	29.6163161839823	26.043134069248573	19.57235109000999	24.76819865675914
42	27.86563406364364	25.291699393247324	19.95430858668253	26.888357956426507
43	28.210075875845504	23.625240731869724	23.53145152069304	24.633231871591736
44	27.370681118923972	24.2828114642551	21.270122643405944	27.07638477341498
45	26.80540494893159	24.59250269223612	22.502460565560153	26.09963179327214
46	28.07137246871887	25.200376352563737	21.74751346491723	24.980737713800167
47	28.85905237772465	24.52778439990225	21.503287853661927	25.109875368711172
48	29.735290227164196	25.251642805844376	20.911257470441452	24.10180949654998
49	28.521710147245326	25.795919160425886	21.060797081249856	24.621573611078933
50	25.65684209110333	25.459398986777586	22.736746762403904	26.147012159715178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	8.5
2	17.0
3	17.5
4	18.0
5	47.0
6	76.0
7	821.5
8	1567.0
9	1493.5
10	1420.0
11	1476.0
12	1532.0
13	1731.5
14	1931.0
15	2318.5
16	2706.0
17	3612.0
18	4518.0
19	5903.0
20	7288.0
21	8842.5
22	10397.0
23	12459.0
24	14521.0
25	16657.5
26	18794.0
27	20559.5
28	22325.0
29	25012.0
30	27699.0
31	28027.0
32	28355.0
33	26429.5
34	24504.0
35	23815.0
36	23126.0
37	23031.5
38	22937.0
39	23119.0
40	23301.0
41	23441.5
42	23582.0
43	26162.0
44	28742.0
45	32500.0
46	36258.0
47	44163.5
48	52069.0
49	55891.5
50	59714.0
51	68727.0
52	77740.0
53	95722.5
54	113705.0
55	112085.0
56	110465.0
57	117589.0
58	124713.0
59	118745.0
60	112777.0
61	104986.5
62	97196.0
63	90659.0
64	84122.0
65	69929.5
66	55737.0
67	49378.5
68	43020.0
69	36225.5
70	29431.0
71	30373.0
72	31315.0
73	22480.0
74	13645.0
75	9082.5
76	4520.0
77	3164.0
78	1808.0
79	1095.5
80	383.0
81	243.0
82	103.0
83	65.0
84	27.0
85	14.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1338107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.443270487159197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2060091986573	13.641685721734396
2	8.11324275098991	2.830429756670012
3	2.8409136238636035	1.4866447431512544
4	1.388274507328334	0.9686419096702321
5	0.8851625267491137	0.772006468959104
6	0.6060704248610882	0.6343110213071674
7	0.4593279217489265	0.5608526826959853
8	0.3864596252988818	0.5392895821163669
9	0.31937196366098974	0.5013802393338439
>10	4.519760770381821	20.119973376174986
>50	1.4764188253092134	17.716994298699042
>100	0.72934138272894	24.783569462917765
>500	0.04239350860464775	4.915212383336633
>1k	0.025522622527287934	8.076570985898408
>5k	0.0012977604674892168	1.445686210294087
>10k+	4.3258682249640567E-4	1.006751157040749
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	13342	0.9970802036010574	No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	8668	0.6477807828521934	No Hit
TATGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	5293	0.395558800604137	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5198	0.3884592188815992	No Hit
TATGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4479	0.33472659510786507	No Hit
TATGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4341	0.32441351850038896	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	4163	0.3111111443255285	No Hit
TATGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	3827	0.2860010447594998	No Hit
TATGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	3244	0.2424320327148726	No Hit
TATGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	3011	0.2250193743848586	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAA	2959	0.22113328754725892	No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2824	0.21104440825733664	No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2728	0.20387009409561416	No Hit
TATGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	2658	0.1986388233526915	No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2562	0.19146450919096902	No Hit
TATGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	2435	0.18197348941452365	No Hit
TATGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2377	0.1776390079418163	No Hit
TATGTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	2190	0.16366404181429436	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA	2137	0.15970322253751007	No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2027	0.15148265422720306	No Hit
TATGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1991	0.14879228641655712	No Hit
TATGTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT	1977	0.1477460322679726	No Hit
TATGTAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA	1937	0.14475673470058822	No Hit
TATGTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1842	0.13765715297805034	No Hit
TATGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1779	0.13294900930941994	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	1774	0.1325753471134969	No Hit
TATGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTC	1720	0.128539795397528	No Hit
TATGTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA	1673	0.12502737075585135	No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA	1664	0.12435477880318989	No Hit
TATGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT	1585	0.11845091610760575	No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	1579	0.1180025214724981	No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	1553	0.11605947805369825	No Hit
TATGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT	1542	0.11523742122266756	No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1497	0.11187446145936014	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1497	0.11187446145936014	No Hit
TATGTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1462	0.10925882608789879	No Hit
TATGTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1432	0.10701685291236052	No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1408	0.1052232743719299	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAA	1377	0.10290656875720701	No Hit
TATGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1350	0.10088879289922256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.07024849283353274	0.0	0.0	0.0	0.0
7	0.07151894429967111	0.0	0.0	0.0	0.0
8	0.07555449601564	0.0	0.0	0.0	0.0
9	0.08183202090714718	0.0	0.0	0.0	0.0
10	0.1310806983298047	0.0	0.0	0.0	0.0
11	0.24288042734998022	0.0	0.0	0.0	0.0
12	0.3058051411434213	0.0	0.0	0.0	0.0
13	0.3501214775798946	0.0	0.0	0.0	0.0
14	0.40026694427276743	0.0	0.0	0.0	0.0
15	0.45183232731014783	0.0	0.0	0.0	0.0
16	0.4938319581318983	0.0	0.0	0.0	0.0
17	0.5320202345552336	0.0	0.0	0.0	0.0
18	0.5703579758569381	0.0	0.0	0.0	0.0
19	0.6144501149758577	0.0	0.0	0.0	0.0
20	0.6594390433649925	0.0	0.0	0.0	0.0
21	0.7015881390651121	0.0	0.0	0.0	0.0
22	0.7550965655212923	0.0	0.0	0.0	0.0
23	0.8186191388282102	0.0	0.0	0.0	0.0
24	0.8864761936078356	0.0	0.0	0.0	0.0
25	0.9580698703466912	0.0	0.0	0.0	0.0
26	1.0308592661125007	0.0	0.0	0.0	0.0
27	1.1078336784726484	0.0	0.0	0.0	0.0
28	1.1858543449813803	0.0	0.0	0.0	0.0
29	1.2422773365657604	0.0	0.0	0.0	0.0
30	1.3004939066905712	0.0	0.0	0.0	0.0
31	1.350041513869967	0.0	0.0	0.0	0.0
32	1.4027278834951167	0.0	0.0	0.0	0.0
33	1.4536206745798355	0.0	0.0	0.0	0.0
34	1.5050365927388467	0.0	0.0	0.0	0.0
35	1.562206908715073	0.0	0.0	0.0	0.0
36	1.6032350178274233	0.0	0.0	0.0	0.0
37	1.647476621824712	0.0	0.0	0.0	0.0
38	1.6943338611934622	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	20	7.856945E-4	44.0	9
AACCGTA	20	7.856945E-4	44.0	23
AGCGTAC	20	7.856945E-4	44.0	11
ACTATCG	20	7.856945E-4	44.0	22
CGTATTT	20	7.856945E-4	44.0	17
ATCTATA	35	1.4463149E-7	44.0	36
GGTAAAT	25	4.4432323E-5	44.0	8
CCAATTG	20	7.856945E-4	44.0	30
TATATCG	30	2.5280297E-6	44.0	20
TCGATAC	25	4.4432323E-5	44.0	25
CATTAGG	30	2.5280297E-6	44.0	33
TAGTTCA	30	2.5280297E-6	44.0	13
CTAAATG	20	7.856945E-4	44.0	21
TACGACA	35	1.4463149E-7	44.0	38
CGAATAT	30	2.5280297E-6	44.0	41
GATGCAT	25	4.4432323E-5	44.0	9
CGTACCC	20	7.856945E-4	44.0	43
GTTCAAC	25	4.4432323E-5	44.0	13
TTAGTGG	30	2.5280297E-6	44.0	18
CATAACA	20	7.856945E-4	44.0	10
>>END_MODULE