FastQCFastQC Report
Fri 17 Jun 2016
SRR1527446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087409
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG111431.0247294256346968No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT67780.6233165257966413No Hit
TATGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG46600.42854160669996294No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC42290.3889061061661252No Hit
TATGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC38820.3569953899590678No Hit
TATGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC34830.32030266440686067No Hit
TATGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG33770.3105547222802092No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA33190.30522094262600363No Hit
TATGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG25400.23358276416693258No Hit
TATGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT25320.23284707042152492No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23520.21629396114985255No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA22950.21105214321382296No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC22690.20866113854124804No Hit
TATGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA21550.19817750266918885No Hit
TATGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG21480.19753377064195715No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC20440.18796975195165758No Hit
TATGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA18650.17150860439816112No Hit
TATGTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT18300.1682899442620026No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA17790.16359989663502877No Hit
TATGTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA17130.15753042323541558No Hit
TATGTAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG17070.15697865292635982No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT16840.1548635334083128No Hit
TATGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA15520.14272458660908638No Hit
TATGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG15420.1418049694273268No Hit
TATGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTC14610.1343560702550742No Hit
TATGTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG14330.13178114214614742No Hit
TATGTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT14190.130493678091684No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG14160.1302177929371561No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA13970.12847052029181294No Hit
TATGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA13900.12782678826458121No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA13390.12313674063760738No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA13150.12092965940138439No Hit
TATGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG12840.11807884613792971No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC12530.115228032874475No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA12300.113112913356428No Hit
TATGTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA11950.10989425322026945No Hit
TATGTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT11840.10888267432033394No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT11560.10630774621140711No Hit
TATGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC11490.1056640141841754No Hit
TATGTAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG11230.10327300951160051No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC11210.1030890860752486No Hit
TATGTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC11190.10290516263889668No Hit
TATGTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT11180.10281320092072072No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA11040.10152573686625732No Hit
TATGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG10890.10014631109361795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCT206.311305E-446.022
AACGTAT501.6370905E-1146.026
CATATGC253.4170334E-546.033
CGTATAT253.4170334E-546.018
TTAATAG453.092282E-1046.046
GTCGAAT206.311305E-446.033
ATAGGCC206.311305E-446.033
TAGACTA253.4170334E-546.025
ACCGCAA253.4170334E-546.020
ATTGTCG253.4170334E-546.014
CATTATC253.4170334E-546.040
TAGCATA253.4170334E-546.030
CGTTAGT501.6370905E-1146.015
ATATCTA206.311305E-446.045
TACGCTT1750.046.011
GCCGAAT253.4170334E-546.018
GTTAACG206.311305E-446.028
CAGATAG206.311305E-446.014
CTAAACT206.311305E-446.032
GTATGCG453.092282E-1046.043
Produced by FastQC (version 0.11.5)