##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527446_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1087409
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73152052263684	33.0	31.0	33.0	30.0	34.0
2	30.88497336328833	31.0	31.0	33.0	28.0	34.0
3	32.09653589403803	33.0	31.0	34.0	30.0	34.0
4	35.97489353132078	37.0	35.0	37.0	35.0	37.0
5	35.60529018979979	37.0	35.0	37.0	35.0	37.0
6	35.93038589895798	37.0	35.0	37.0	35.0	37.0
7	36.39757993542448	37.0	36.0	37.0	35.0	37.0
8	36.69660541709697	37.0	37.0	37.0	35.0	37.0
9	38.76111012507713	39.0	39.0	39.0	39.0	39.0
10	37.85531570917659	39.0	39.0	39.0	35.0	39.0
11	37.52585549687376	39.0	37.0	39.0	35.0	39.0
12	37.42370074185518	39.0	37.0	39.0	35.0	39.0
13	37.35086154335673	39.0	37.0	39.0	35.0	39.0
14	38.17354647607294	40.0	38.0	40.0	35.0	40.0
15	38.304165222101346	40.0	38.0	40.0	35.0	40.0
16	38.42965250425553	40.0	38.0	40.0	35.0	40.0
17	38.38769405072057	40.0	38.0	40.0	35.0	40.0
18	38.273754401517735	40.0	38.0	40.0	35.0	40.0
19	38.22244895894737	40.0	38.0	40.0	34.0	40.0
20	38.180649599184854	40.0	38.0	40.0	34.0	40.0
21	38.13220048758103	40.0	38.0	40.0	34.0	40.0
22	38.10510396732048	40.0	38.0	40.0	34.0	40.0
23	37.89705621343947	40.0	37.0	40.0	34.0	40.0
24	37.81780360471543	40.0	37.0	40.0	34.0	40.0
25	37.69439833586075	39.0	37.0	40.0	34.0	40.0
26	37.481277973605145	39.0	36.0	40.0	34.0	40.0
27	37.37883261955713	39.0	36.0	40.0	33.0	40.0
28	37.23786818023393	39.0	35.0	40.0	33.0	40.0
29	37.11937826521576	39.0	35.0	40.0	33.0	40.0
30	36.98884504358526	39.0	35.0	40.0	33.0	40.0
31	36.84069839407252	39.0	35.0	40.0	33.0	40.0
32	36.66035870587792	39.0	35.0	40.0	32.0	40.0
33	36.332757039899434	38.0	35.0	40.0	31.0	40.0
34	35.90772653159942	38.0	35.0	40.0	30.0	40.0
35	35.68763179263736	38.0	35.0	40.0	29.0	40.0
36	35.43816172203835	38.0	35.0	40.0	28.0	40.0
37	35.12802726481021	38.0	34.0	40.0	26.0	40.0
38	35.07890223457779	38.0	34.0	40.0	26.0	40.0
39	34.831944558119346	38.0	34.0	40.0	25.0	40.0
40	34.46242398214471	38.0	34.0	40.0	23.0	40.0
41	34.247304372135964	37.0	34.0	40.0	23.0	40.0
42	33.99092981573631	37.0	33.0	40.0	22.0	40.0
43	33.84168054522264	36.0	33.0	40.0	22.0	40.0
44	33.684267832986485	36.0	33.0	39.0	21.0	40.0
45	33.45980491241106	35.0	33.0	39.0	20.0	40.0
46	33.24484071770603	35.0	33.0	39.0	20.0	40.0
47	33.05767011308532	35.0	33.0	39.0	20.0	40.0
48	32.77984272706958	35.0	33.0	39.0	20.0	40.0
49	32.500664423413824	35.0	32.0	38.0	18.0	40.0
50	32.81782383629343	35.0	33.0	39.0	20.0	40.0
51	32.88487036616397	35.0	33.0	39.0	20.0	40.0
52	32.494113070610965	35.0	33.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	4.0
14	10.0
15	17.0
16	66.0
17	143.0
18	326.0
19	610.0
20	1153.0
21	1842.0
22	2907.0
23	4709.0
24	6659.0
25	9524.0
26	13993.0
27	18424.0
28	22080.0
29	20586.0
30	18304.0
31	19657.0
32	26636.0
33	41396.0
34	76389.0
35	84144.0
36	147274.0
37	246449.0
38	285379.0
39	38722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.45089658077136	0.45796935651626935	0.07687999639510065	0.014254066317273445
8	98.95411937918483	0.9214564161230963	0.08074238855849088	0.04368181613357991
9	98.48318342040575	0.8290348893562588	0.3220499370522039	0.3657317531857838
10	70.51550980357896	19.116910012699915	3.7451409727158778	6.6224392110052435
11	34.56601885766993	32.221914661364764	14.53151482101031	18.680551659954993
12	31.602552489449693	23.142718149288815	24.234303744037433	21.020425617224063
13	23.402050194545016	22.357548999502487	28.156103177369324	26.084297628583176
14	25.100307244100428	22.778641707030197	27.00483442752451	25.11621662134487
15	24.517913682892086	22.55131233969923	25.867267973687913	27.06350600372077
16	28.519995696191593	22.389459715709545	23.503575931411273	25.586968656687592
17	30.320790061513193	22.719234437088527	20.81158055524646	26.148394946151814
18	29.523665888363993	25.182521020149736	20.36253148539326	24.931281606093016
19	30.733422290968715	23.23789852760093	19.698108071571966	26.330571109858386
20	28.458473306731875	24.40765158279911	20.559881332598863	26.573993777870147
21	31.84146903327083	23.251232976736443	22.230365943265138	22.67693204672759
22	31.201875283357044	22.82940457546333	21.744624147859728	24.2240959933199
23	30.07828701068319	25.482224259685175	19.69709649267203	24.7423922369596
24	28.731231762841762	22.463396937123015	23.537417843700027	25.267953456335196
25	29.46793708714936	23.07301116691144	21.486211719785288	25.972840026153914
26	28.69987281694376	22.05012097564026	22.989601888525847	26.260404318890135
27	29.980255819107622	22.642446402411604	21.18356570526821	26.193732073212562
28	32.18761294048513	23.497414496293484	19.079389631684123	25.23558293153726
29	32.09335217935478	24.770532522721442	18.220743069075205	24.915372228848575
30	32.06622347249287	23.135912982143793	18.61581061035912	26.18205293500422
31	32.87502678385042	24.54789320301745	17.841125096444852	24.73595491668728
32	34.48067838320264	24.768693288357923	18.15213962731594	22.598488701123497
33	33.36178015815576	26.11436911042671	18.211914744130315	22.311935987287214
34	30.390129197017867	28.014758016532877	19.809933520873933	21.785179265575326
35	31.070553949801777	28.728013102705606	20.050045567031354	20.151387380461262
36	32.42312690073376	28.507948711110537	16.98349011273587	22.08543427541983
37	31.734609516750368	28.80526094597341	17.297263495152237	22.162866042123987
38	33.80062147729143	27.42224866632518	17.404858705418107	21.37227115096528
39	32.43434623035123	27.131649636889154	16.780438639003357	23.65356549375626
40	31.195805809957434	26.644712339147457	18.89767327656843	23.26180857432668
41	30.08831083796437	25.653181093774286	19.50462061652975	24.753887451731593
42	28.594300764477765	24.647579705520187	20.107521640891328	26.65059788911072
43	29.008772228296802	22.887064572759652	23.573098990352296	24.531064208591246
44	28.067360119329525	23.455663876241598	21.410527225726476	27.0664487787024
45	27.658590282037395	23.761252665740308	22.47691530969488	26.103241742527423
46	28.6844232482902	24.377855986110102	21.80982500604648	25.127895759553216
47	29.312613745150166	23.76566682821275	21.57992071060659	25.34179871603049
48	30.210344037983866	24.4306420123431	20.983916815108206	24.375097134564825
49	29.11195327609023	24.736138840123633	21.399767704699887	24.75214017908625
50	25.608487698740767	25.35108684956626	22.696979701289948	26.34344575040302
51	26.662093103882718	25.696587024753338	21.532284540591444	26.109035330772507
52	28.560458852189015	24.64537262428396	20.905933278094995	25.888235245432035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	13.0
2	26.0
3	21.5
4	17.0
5	40.5
6	64.0
7	621.5
8	1179.0
9	1135.5
10	1092.0
11	1193.5
12	1295.0
13	1256.0
14	1379.0
15	1541.0
16	1850.0
17	2159.0
18	2639.5
19	3120.0
20	3713.5
21	4307.0
22	5446.0
23	6585.0
24	8341.0
25	10097.0
26	13458.5
27	16820.0
28	19950.0
29	23080.0
30	24049.5
31	25019.0
32	24093.0
33	23167.0
34	22215.5
35	21264.0
36	20668.5
37	20073.0
38	18580.5
39	18275.0
40	19462.0
41	20123.5
42	20785.0
43	21513.5
44	22242.0
45	25741.5
46	29241.0
47	30620.0
48	31999.0
49	40448.5
50	48898.0
51	53329.0
52	57760.0
53	64678.5
54	71597.0
55	88050.0
56	104503.0
57	97298.5
58	90094.0
59	92678.5
60	95263.0
61	86415.0
62	77567.0
63	74082.5
64	60571.0
65	50544.0
66	43970.5
67	37397.0
68	32739.5
69	28082.0
70	28704.0
71	29326.0
72	21078.0
73	12830.0
74	10118.0
75	7406.0
76	4503.5
77	1601.0
78	1156.5
79	712.0
80	437.5
81	163.0
82	140.0
83	117.0
84	63.0
85	9.0
86	5.5
87	2.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1087409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.177450036233644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.94551842876557	12.12427379820983
2	8.701070091553314	2.815222533357413
3	3.1443854362171995	1.5260441486719345
4	1.627417324225388	1.0530984980302907
5	1.0217704721016203	0.8264820380461413
6	0.753715420546672	0.731591613445957
7	0.5749050630637396	0.651034855330398
8	0.4956779178380282	0.6415043799911222
9	0.3994213040108779	0.5815456370138941
>10	5.987992599287234	24.57625180834902
>50	1.521498536957507	16.653628136524897
>100	0.7596035617491161	23.93717515444912
>500	0.038381175442828645	4.218107311853819
>1k	0.027496961511280223	8.003246055270582
>5k	5.728533648183381E-4	0.6281380472744791
>10k+	5.728533648183381E-4	1.0326559841811036
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	11143	1.0247294256346968	No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6778	0.6233165257966413	No Hit
TATGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4660	0.42854160669996294	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4229	0.3889061061661252	No Hit
TATGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3882	0.3569953899590678	No Hit
TATGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3483	0.32030266440686067	No Hit
TATGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3377	0.3105547222802092	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	3319	0.30522094262600363	No Hit
TATGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2540	0.23358276416693258	No Hit
TATGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2532	0.23284707042152492	No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2352	0.21629396114985255	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	2295	0.21105214321382296	No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2269	0.20866113854124804	No Hit
TATGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2155	0.19817750266918885	No Hit
TATGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2148	0.19753377064195715	No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2044	0.18796975195165758	No Hit
TATGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1865	0.17150860439816112	No Hit
TATGTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1830	0.1682899442620026	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA	1779	0.16359989663502877	No Hit
TATGTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA	1713	0.15753042323541558	No Hit
TATGTAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG	1707	0.15697865292635982	No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1684	0.1548635334083128	No Hit
TATGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1552	0.14272458660908638	No Hit
TATGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1542	0.1418049694273268	No Hit
TATGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTC	1461	0.1343560702550742	No Hit
TATGTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1433	0.13178114214614742	No Hit
TATGTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	1419	0.130493678091684	No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1416	0.1302177929371561	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA	1397	0.12847052029181294	No Hit
TATGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	1390	0.12782678826458121	No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1339	0.12313674063760738	No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	1315	0.12092965940138439	No Hit
TATGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG	1284	0.11807884613792971	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1253	0.115228032874475	No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA	1230	0.113112913356428	No Hit
TATGTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1195	0.10989425322026945	No Hit
TATGTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1184	0.10888267432033394	No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1156	0.10630774621140711	No Hit
TATGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1149	0.1056640141841754	No Hit
TATGTAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	1123	0.10327300951160051	No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1121	0.1030890860752486	No Hit
TATGTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	1119	0.10290516263889668	No Hit
TATGTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1118	0.10281320092072072	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA	1104	0.10152573686625732	No Hit
TATGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1089	0.10014631109361795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.07789157529503618	0.0	0.0	0.0	0.0
7	0.0798227713767313	0.0	0.0	0.0	0.0
8	0.08423693384917727	0.0	0.0	0.0	0.0
9	0.08828324944891941	0.0	0.0	0.0	0.0
10	0.13766669210940868	0.0	0.0	0.0	0.0
11	0.25933204525620074	0.0	0.0	0.0	0.0
12	0.3263721378064739	0.0	0.0	0.0	0.0
13	0.3729967289216845	0.0	0.0	0.0	0.0
14	0.42440332938204484	0.0	0.0	0.0	0.0
15	0.4816954798056665	0.0	0.0	0.0	0.0
16	0.5310789224661557	0.0	0.0	0.0	0.0
17	0.5721858104908089	0.0	0.0	0.0	0.0
18	0.6169711672425003	0.0	0.0	0.0	0.0
19	0.6602851365033764	0.0	0.0	0.0	0.0
20	0.7058981487186514	0.0	0.0	0.0	0.0
21	0.7533503952974456	0.0	0.0	0.0	0.0
22	0.8052168043486857	0.0	0.0	0.0	0.0
23	0.8573590985544538	0.0	0.0	0.0	0.0
24	0.9144673255417235	0.0	0.0	0.0	0.0
25	0.9628391893022773	0.0	0.0	0.0	0.0
26	1.0124985171172944	0.0	0.0	0.0	0.0
27	1.0639051175776548	0.0	0.0	0.0	0.0
28	1.1238641578283792	0.0	0.0	0.0	0.0
29	1.1610166919714662	0.0	0.0	0.0	0.0
30	1.2054342018504538	0.0	0.0	0.0	0.0
31	1.2381725735210947	0.0	0.0	0.0	0.0
32	1.2755090311005335	0.0	0.0	0.0	0.0
33	1.3075117090257669	0.0	0.0	0.0	0.0
34	1.3432848173962144	0.0	0.0	0.0	0.0
35	1.3843917054208674	0.0	0.0	0.0	0.0
36	1.4225558184638898	0.0	0.0	0.0	0.0
37	1.4558459604435865	0.0	0.0	0.0	0.0
38	1.4920788774049139	0.0	0.0	0.0	0.0
39	1.5293233732661768	0.0	0.0	0.0	0.0
40	1.5713498784725894	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCT	20	6.311305E-4	46.0	22
AACGTAT	50	1.6370905E-11	46.0	26
CATATGC	25	3.4170334E-5	46.0	33
CGTATAT	25	3.4170334E-5	46.0	18
TTAATAG	45	3.092282E-10	46.0	46
GTCGAAT	20	6.311305E-4	46.0	33
ATAGGCC	20	6.311305E-4	46.0	33
TAGACTA	25	3.4170334E-5	46.0	25
ACCGCAA	25	3.4170334E-5	46.0	20
ATTGTCG	25	3.4170334E-5	46.0	14
CATTATC	25	3.4170334E-5	46.0	40
TAGCATA	25	3.4170334E-5	46.0	30
CGTTAGT	50	1.6370905E-11	46.0	15
ATATCTA	20	6.311305E-4	46.0	45
TACGCTT	175	0.0	46.0	11
GCCGAAT	25	3.4170334E-5	46.0	18
GTTAACG	20	6.311305E-4	46.0	28
CAGATAG	20	6.311305E-4	46.0	14
CTAAACT	20	6.311305E-4	46.0	32
GTATGCG	45	3.092282E-10	46.0	43
>>END_MODULE