##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1470484
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.835557544318743	31.0	31.0	33.0	28.0	33.0
2	29.53368958791799	31.0	30.0	31.0	25.0	33.0
3	30.931219244820074	31.0	31.0	33.0	28.0	34.0
4	35.19553697966112	35.0	35.0	37.0	33.0	37.0
5	34.941884440769165	35.0	35.0	37.0	33.0	37.0
6	35.1173633987177	35.0	35.0	37.0	33.0	37.0
7	36.070862382725686	37.0	35.0	37.0	35.0	37.0
8	36.489694549549675	37.0	37.0	37.0	35.0	37.0
9	38.629882406064944	39.0	39.0	39.0	37.0	39.0
10	37.42030583127732	39.0	37.0	39.0	34.0	39.0
11	36.805776873464794	39.0	37.0	39.0	32.0	39.0
12	36.71279660302322	39.0	35.0	39.0	33.0	39.0
13	36.60063285285661	39.0	35.0	39.0	32.0	39.0
14	37.44760160600183	39.0	37.0	40.0	33.0	40.0
15	37.58081080787006	40.0	37.0	40.0	33.0	40.0
16	37.70390089249526	40.0	37.0	40.0	33.0	40.0
17	37.705807747653154	40.0	37.0	40.0	33.0	40.0
18	37.60471314206751	40.0	37.0	40.0	33.0	40.0
19	37.49993675551723	39.0	37.0	40.0	33.0	40.0
20	37.43188705215426	39.0	36.0	40.0	33.0	40.0
21	37.46598194880053	39.0	37.0	40.0	33.0	40.0
22	37.43816525715343	39.0	36.0	40.0	33.0	40.0
23	37.33229059275722	39.0	36.0	40.0	33.0	40.0
24	37.27686802440557	39.0	36.0	40.0	33.0	40.0
25	37.167680165170104	39.0	36.0	40.0	33.0	40.0
26	36.90606086159387	39.0	35.0	40.0	32.0	40.0
27	36.76184167933823	39.0	35.0	40.0	32.0	40.0
28	36.55082748265197	38.0	35.0	40.0	31.0	40.0
29	36.475032030270306	38.0	35.0	40.0	31.0	40.0
30	36.35090827237835	38.0	35.0	40.0	31.0	40.0
31	36.15270006338049	38.0	35.0	40.0	30.0	40.0
32	35.988745202259935	38.0	35.0	40.0	30.0	40.0
33	35.77336645621442	38.0	35.0	40.0	30.0	40.0
34	35.42795025311394	38.0	34.0	40.0	28.0	40.0
35	35.18802448717565	38.0	34.0	40.0	27.0	40.0
36	35.000536558031236	38.0	34.0	40.0	26.0	40.0
37	34.72918304449419	37.0	34.0	40.0	26.0	40.0
38	34.47569099697787	37.0	33.0	40.0	24.0	40.0
39	34.15992693562119	37.0	33.0	40.0	23.0	40.0
40	34.17724096283945	37.0	33.0	40.0	23.0	40.0
41	34.2015560862954	37.0	33.0	40.0	23.0	40.0
42	34.14759834177046	37.0	33.0	40.0	23.0	40.0
43	34.00264538750507	36.0	33.0	40.0	23.0	40.0
44	33.84689666803583	36.0	33.0	40.0	23.0	40.0
45	33.68023181483103	35.0	33.0	39.0	23.0	40.0
46	33.3888515617987	35.0	33.0	39.0	21.0	40.0
47	33.26844834761888	35.0	33.0	39.0	21.0	40.0
48	33.085067909613436	35.0	33.0	39.0	20.0	40.0
49	32.8319084056678	35.0	33.0	38.0	20.0	40.0
50	32.61389583293664	35.0	33.0	38.0	20.0	40.0
51	32.422655397814594	35.0	32.0	38.0	20.0	40.0
52	31.873351223134694	35.0	31.0	37.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	9.0
14	14.0
15	25.0
16	129.0
17	316.0
18	621.0
19	1300.0
20	2236.0
21	3602.0
22	5397.0
23	7683.0
24	10837.0
25	14857.0
26	20592.0
27	27397.0
28	31932.0
29	32193.0
30	32673.0
31	37548.0
32	49827.0
33	73391.0
34	118322.0
35	129671.0
36	213243.0
37	314884.0
38	317369.0
39	24410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.47214658575	0.4559723193179933	0.05984424175985594	0.012036853172152842
8	98.9695229597874	0.9101765133112635	0.07970164925289905	0.040598877648447725
9	98.53388408170372	0.7982405792922602	0.3000372666414596	0.36783807236256905
10	70.79383386694448	18.98782985738029	3.5916065730738995	6.626729702601321
11	34.31523226366285	32.209395001917734	14.597234651992133	18.87813808242728
12	31.368311385911035	23.08784046613224	24.265955970959222	21.277892176997508
13	23.211541234042667	22.131420675097452	28.321083398391277	26.3359546924686
14	24.935395420827426	22.565767461597677	27.143579936945933	25.355257180628964
15	24.50546894763901	22.27287070107529	26.007287396530664	27.214372954755035
16	28.479194605313623	22.026217218276432	23.622018328659138	25.87256984775081
17	30.299683641576515	22.538497528704834	20.724672964819746	26.437145864898902
18	29.61650721803161	24.954300760837928	20.23231806670457	25.19687395442589
19	30.73008614850621	23.01636740012132	19.70589275367838	26.54765369769409
20	28.440771881910994	24.282957176004636	20.484547944758326	26.79172299732605
21	31.939823894717655	22.93775382799133	22.177051909439342	22.945370367851673
22	31.29575024277721	22.577260276208378	21.62342466834049	24.503564812673922
23	30.11158230895406	25.40007235712867	19.594160834119922	24.894184499797344
24	28.776715693608363	22.37134168069833	23.468599454329322	25.383343171363986
25	29.539865785686892	22.853427850966078	21.44375593342056	26.162950429926475
26	28.681372935713682	21.899320223817465	22.807796616624188	26.611510223844665
27	29.99447800860125	22.400379738915895	21.16507218031614	26.440070072166716
28	32.3781829656086	23.362715949306487	18.980757356081398	25.27834372900351
29	32.06434072047027	24.64691897361685	18.20217016982164	25.086570136091247
30	31.955328993719075	23.05621822474777	18.552122974476433	26.436329807056723
31	33.03667363942756	24.437056098536264	17.728380587616048	24.797889674420123
32	34.410098987816255	24.612576539425117	18.199926010755643	22.77739846200299
33	33.298696211587476	26.04775026453875	18.17592030923152	22.477633214642253
34	30.29703145358943	27.989287880724984	19.825785251658637	21.887895414026946
35	30.78693817817807	28.686745316508034	20.183898634735232	20.342417870578664
36	32.28991270901281	28.403641250091805	17.03901572543462	22.26743031546076
37	31.49344025504528	28.83778402213149	17.316475391775768	22.352300331047463
38	33.72631052088972	27.294822657029933	17.40318153750738	21.57568528457297
39	32.23006846725296	27.047217106748526	16.728097687564095	23.99461673843442
40	30.844878284972836	26.559350526765336	18.978037163274134	23.61773402498769
41	29.872681375655908	25.62714045171522	19.705756744038016	24.79442142859086
42	28.167392504780736	24.685885735580936	20.180090364805057	26.966631394833268
43	28.685181205643858	22.967539939230893	23.667581558180846	24.679697296944408
44	27.704007660062945	23.600256786201008	21.40138893044739	27.294346623288657
45	27.277753447164333	23.821816490352838	22.595961601758333	26.304468460724493
46	28.41540608398323	24.534166981755668	21.91441729389779	25.136009640363312
47	29.265602345894276	23.790398263428912	21.604179304229085	25.33982008644773
48	30.189515832882236	24.511385366994812	20.915358480609108	24.383740319513848
49	28.92285805217874	24.84746518833255	21.362966207044757	24.86671055244396
50	25.699973614129767	25.32540306456922	22.549514309574263	26.42510901172675
51	26.746771811185976	25.63992535790937	21.337940433217906	26.275362397686745
52	28.5415550254202	24.74389384719589	20.682986010048392	26.031565117335514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	12.5
2	25.0
3	24.5
4	24.0
5	50.5
6	77.0
7	854.0
8	1631.0
9	1566.0
10	1501.0
11	1613.5
12	1726.0
13	1740.5
14	2001.5
15	2248.0
16	2842.5
17	3437.0
18	4178.0
19	4919.0
20	5906.0
21	6893.0
22	8465.5
23	10038.0
24	12500.0
25	14962.0
26	18918.0
27	22874.0
28	26503.5
29	30133.0
30	30888.5
31	31644.0
32	30191.0
33	28738.0
34	27570.0
35	26402.0
36	25580.5
37	24759.0
38	23358.0
39	23659.0
40	25361.0
41	26092.0
42	26823.0
43	28358.5
44	29894.0
45	34922.5
46	39951.0
47	42315.0
48	44679.0
49	55755.0
50	66831.0
51	72728.5
52	78626.0
53	88267.5
54	97909.0
55	120416.0
56	142923.0
57	132722.0
58	122521.0
59	125398.0
60	128275.0
61	117095.0
62	105915.0
63	100889.0
64	82166.5
65	68470.0
66	59843.5
67	51217.0
68	44834.0
69	38451.0
70	39542.5
71	40634.0
72	28905.0
73	17176.0
74	13495.5
75	9815.0
76	6013.5
77	2212.0
78	1550.5
79	889.0
80	542.5
81	196.0
82	148.0
83	100.0
84	53.0
85	6.0
86	4.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1470484.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.333347517330242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57731005210688	12.834305120580686
2	7.794697506584856	2.5462700633503603
3	2.893449274883904	1.4177913759133802
4	1.449248053051856	0.9468428835724088
5	0.8466358685860539	0.6914198931126379
6	0.6163959925963423	0.6040685973219466
7	0.4467134996364638	0.5107428781171599
8	0.3578206825034534	0.4675527644973755
9	0.3297340435660594	0.4847094649673063
>10	4.1165651431052455	17.14439193971828
>50	1.6404955564182464	18.687506308078596
>100	0.8513067154740758	26.841496143739086
>500	0.0483602247771757	5.250007236893961
>1k	0.029182894262088786	8.679262936728161
>5k	0.0016675939578336448	1.8137379087147545
>10k+	4.168984894584112E-4	1.0798944846939214
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	15859	1.0784884432608584	No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9730	0.661686900367498	No Hit
TATGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5942	0.4040846415193909	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5541	0.3768147086265475	No Hit
TATGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5423	0.3687901398451122	No Hit
TATGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4956	0.33703188882027957	No Hit
TATGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4389	0.2984731557772815	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	4277	0.2908566159169362	No Hit
TATGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3673	0.2497817045272169	No Hit
TATGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	3410	0.23189643681944175	No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3256	0.22142369451146698	No Hit
TATGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3094	0.21040691364203895	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	3066	0.20850277867695263	No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3035	0.20639462925132135	No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2981	0.20272236896151202	No Hit
TATGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2871	0.19524183874153	No Hit
TATGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2561	0.1741603444852171	No Hit
TATGTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	2487	0.16912798779177468	No Hit
TATGTAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA	2338	0.15899526958470817	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA	2294	0.15600305749671536	No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2288	0.15559502857562543	No Hit
TATGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	2247	0.1528068309481776	No Hit
TATGTAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG	2245	0.1526708213078143	No Hit
TATGTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2009	0.13662168374494382	No Hit
TATGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1997	0.135805625902764	No Hit
TATGTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	1900	0.1292091583451435	No Hit
TATGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	1849	0.12574091251587913	No Hit
TATGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGTCTC	1826	0.12417680165170107	No Hit
TATGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG	1766	0.1200965124408018	No Hit
TATGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1750	0.11900843531789534	No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1749	0.11894043049771369	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1690	0.11492814610699607	No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	1690	0.11492814610699607	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA	1672	0.11370405934372628	No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1666	0.11329603042263636	No Hit
TATGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1579	0.10737961106683243	No Hit
TATGTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1564	0.1063595387641076	No Hit
TATGTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1548	0.10527146164120112	No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA	1534	0.10431939415865797	No Hit
TATGTAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	1529	0.10397937005774968	No Hit
TATGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1527	0.10384336041738637	No Hit
TATGTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	1518	0.1032313170357515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.06331248758912032	0.0	0.0	0.0	0.0
7	0.0639245309707552	0.0	0.0	0.0	0.0
8	0.06725676715965628	0.0	0.0	0.0	0.0
9	0.07106503708982893	0.0	0.0	0.0	0.0
10	0.11213994847954822	0.0	0.0	0.0	0.0
11	0.21768342940147598	0.0	0.0	0.0	0.0
12	0.27766368080169523	0.0	0.0	0.0	0.0
13	0.31554236564287674	0.0	0.0	0.0	0.0
14	0.3612416048049486	0.0	0.0	0.0	0.0
15	0.41040908979628477	0.0	0.0	0.0	0.0
16	0.45345614097127207	0.0	0.0	0.0	0.0
17	0.4889546571060957	0.0	0.0	0.0	0.0
18	0.5279894238903653	0.0	0.0	0.0	0.0
19	0.5666841665737268	0.0	0.0	0.0	0.0
20	0.6052428996167248	0.0	0.0	0.0	0.0
21	0.6463858158266258	0.0	0.0	0.0	0.0
22	0.6927651031905141	0.0	0.0	0.0	0.0
23	0.7424766267433036	0.0	0.0	0.0	0.0
24	0.7942962997217243	0.0	0.0	0.0	0.0
25	0.8482241221257762	0.0	0.0	0.0	0.0
26	0.9048041325169128	0.0	0.0	0.0	0.0
27	0.959480007942963	0.0	0.0	0.0	0.0
28	1.014767926750648	0.0	0.0	0.0	0.0
29	1.0513545200083783	0.0	0.0	0.0	0.0
30	1.090729310893556	0.0	0.0	0.0	0.0
31	1.1241876824229302	0.0	0.0	0.0	0.0
32	1.1615903335228401	0.0	0.0	0.0	0.0
33	1.1957287532540306	0.0	0.0	0.0	0.0
34	1.2314312838493993	0.0	0.0	0.0	0.0
35	1.2740703061032965	0.0	0.0	0.0	0.0
36	1.305896561948311	0.0	0.0	0.0	0.0
37	1.3386068804556868	0.0	0.0	0.0	0.0
38	1.3749214544326902	0.0	0.0	0.0	0.0
39	1.4117800669711469	0.0	0.0	0.0	0.0
40	1.4508828385755983	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	20	6.3120335E-4	46.0	9
CGTATTA	20	6.3120335E-4	46.0	17
AATTTCG	30	1.8617611E-6	46.0	12
ATTAGGC	30	1.8617611E-6	46.0	13
GCGAACT	45	3.110472E-10	46.0	19
ACCGCAA	40	5.6134013E-9	46.0	20
TATGGAC	25	3.417625E-5	46.0	39
TATATCG	30	1.8617611E-6	46.0	20
ATGCTAA	25	3.417625E-5	46.0	22
TCGATGA	20	6.3120335E-4	46.0	32
ATATCCC	20	6.3120335E-4	46.0	34
TCAATCA	20	6.3120335E-4	46.0	18
CACTAGA	20	6.3120335E-4	46.0	42
CTACGAA	20	6.3120335E-4	46.0	36
CGCTAAA	60	0.0	46.0	33
CGAATTA	30	1.8617611E-6	46.0	41
GTACTAG	30	1.8617611E-6	46.0	41
ACTAATG	40	5.6134013E-9	46.0	24
TCGTATA	30	1.8617611E-6	46.0	17
ACTAACA	30	1.8617611E-6	46.0	42
>>END_MODULE