FastQCFastQC Report
Fri 17 Jun 2016
SRR1527444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299400
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC84032.806613226452906No Hit
GGTACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT33251.110554442217769No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG28930.9662658650634602No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG25350.8466933867735471No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT16600.554442217768871No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT14350.4792919171676686No Hit
GGTACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC13300.4442217768871075No Hit
GGTACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA12890.4305277221108884No Hit
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC11430.3817635270541082No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT11090.3704074816299265No Hit
GGTACAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG10810.3610554442217769No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT9930.3316633266533066No Hit
GGTACAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8620.2879091516366066No Hit
GGTACAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA7810.2608550434201737No Hit
GGTACAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG7440.24849699398797595No Hit
GGTACAGGGGAGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTG7160.23914495657982632No Hit
GGTACAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT6480.21643286573146295No Hit
GGTACAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA6460.21576486305945222No Hit
GGTACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA5900.19706078824315298No Hit
GGTACAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG5880.19639278557114231No Hit
GGTACAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT5840.19505678022712092No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC5780.19305277221108885No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACT5760.19238476953907815No Hit
GGTACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG5630.1880427521710087No Hit
GGTACAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG5550.18537074148296592No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGC5270.1760187040748163No Hit
GGTACAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC5160.17234468937875752No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC5110.1706746826987308No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCAT5100.17034068136272545No Hit
GGTACAGGGGGGGATGAGAAGAGATGGCTCAGCAGTTAGAAGCAGTGACT4970.16599866399465596No Hit
GGTACAGGGGCGTTCCCAGGGCCTCATGGAGATTATAATGTCAGCAAAAC4870.16265865063460255No Hit
GGTACAGGGAGCACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTT4670.15597862391449566No Hit
GGTACAGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC4630.1546426185704743No Hit
GGTACAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA4580.15297261189044756No Hit
GGTACAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG4580.15297261189044756No Hit
GGTACAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC4540.1516366065464262No Hit
GGTACAGGGGGTGAAGACCACAGTTTCCTGTCGCGTGGATGTGTTTCCTC4490.14996659986639946No Hit
GGTACAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA4460.14896459585838345No Hit
GGTACAGGGGGTTTTCCGCAGCCTTGCTGCCTTCTCTCTGAACGCGGCTT4280.14295257181028723No Hit
GGTACAGGGGGTTTGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTC4120.13760855043420173No Hit
GGTACAGGGATCTACATCGAACGAGTCCAGCGAGAGAAGGCTAATGGCAC4030.13460253841015365No Hit
GGTACAGGGGGAGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACT4010.13393453573814296No Hit
GGTACAGGGGACTGTCGTCCTGCGGCCGTGCCGCTTCCAGTCTCCAGACC3980.13293253173012692No Hit
GGTACAGGGGGCCGGCGCGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCG3960.13226452905811623No Hit
GGTACAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT3930.13126252505010022No Hit
GGTACAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT3920.13092852371409486No Hit
GGTACAGGGGTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGT3820.12758851035404142No Hit
GGTACAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGAC3800.12692050768203073No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC3770.1259185036740147No Hit
GGTACAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA3710.12391449565798264No Hit
GGTACAGGGGGTCAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTT3590.1199064796259185No Hit
GGTACAGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACT3510.11723446893787574No Hit
GGTACAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC3490.11656646626586507No Hit
GGTACAGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGC3470.11589846359385439No Hit
GGTACAGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTG3460.11556446225784903No Hit
GGTACAGGGGCCATCAGCCTGCTGCCTATCTCCTATAGCCATTGCTGGCC3430.114562458249833No Hit
GGTACAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA3420.11422845691382766No Hit
GGTACAGGGGCTGTTATCCTAGGCCTCTAGACTGCAAGAGTACTGGCCCT3410.11389445557782232No Hit
GGTACAGGGGTTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGT3410.11389445557782232No Hit
GGTACAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCA3380.11289245156980628No Hit
GGTACAGGGGAGCCGCTCCAAGATGGCGACTCCCATGCATCGGCTCATAG3350.11189044756179024No Hit
GGTACAGGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAAC3340.11155644622578491No Hit
GGTACAGGGAGCGTGTGGAAAATACTACAGAGTATGCACACTGGCTATCA3280.10955243820975284No Hit
GGTACAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACT3260.10888443553774216No Hit
GGTACAGGGGGTAAAGGGCTGGGGCTCAGCCCGGCAGGTTTAAAGGGTTG3240.10821643286573147No Hit
GGTACAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGA3220.10754843019372076No Hit
GGTACAGGGGTTTCTCTCTGAGCAATGTCTGGACCCTGAGCTCCCTTTGC3210.10721442885771543No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGGCTGC3200.10688042752171009No Hit
GGTACAGGGGGAGTTCGGCAGTGAGGCAACATGTCGTCGCCGACGATCCC3130.10454241816967268No Hit
GGTACAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT3120.10420841683366734No Hit
GGTACAGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAA3090.10320641282565131No Hit
GGTACAGGGGGCTGGAGGACTCTTAACTGCCGCCGCGATGCCGAAGGGTC3070.10253841015364061No Hit
GGTACAGGGGGTTGAAGAAGACCGAGAGTTCCTCGGGGACTCGCTCAGCT3050.10187040748162993No Hit
GGTACAGGGGTACAGGGGGCATCTAATACAACAGTTAGGGAAGGATGAAT3030.10120240480961924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAA207.847145E-444.00000433
TTGATGC207.847145E-444.00000437
AAACTTT207.847145E-444.00000418
ACAACAT207.847145E-444.00000443
AACGTCT207.847145E-444.00000429
AACCGTT207.847145E-444.00000412
TGATATA207.847145E-444.00000427
CCCCTAT207.847145E-444.00000436
ACCGGTA207.847145E-444.00000429
GTCGCAA207.847145E-444.00000410
AAATCCA207.847145E-444.00000415
CGTATTC207.847145E-444.00000426
CCCTCGT207.847145E-444.00000430
GTACATA207.847145E-444.00000433
CTACTGA207.847145E-444.00000422
TTTACTA207.847145E-444.00000414
ATAGGCG207.847145E-444.00000437
ACGTTTG207.847145E-444.00000444
CCGATTC207.847145E-444.00000420
TAAAGCT207.847145E-444.00000422
Produced by FastQC (version 0.11.5)