FastQCFastQC Report
Fri 17 Jun 2016
SRR1527443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263500
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC74342.8212523719165086No Hit
GGTACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG27741.052751423149905No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT27141.0299810246679317No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT22060.8371916508538899No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC14190.5385199240986717No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12290.46641366223908914No Hit
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA11600.4402277039848197No Hit
GGTACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT11510.43681214421252373No Hit
GGTACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11030.41859582542694496No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT9780.371157495256167No Hit
GGTACAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG9330.3540796963946869No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT8310.31537001897533207No Hit
GGTACAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8010.3039848197343454No Hit
GGTACAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG6950.2637571157495256No Hit
GGTACAGGGGAGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGCT6390.2425047438330171No Hit
GGTACAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA6380.24212523719165086No Hit
GGTACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG5620.2132827324478178No Hit
GGTACAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG5420.20569259962049335No Hit
GGTACAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC5340.20265654648956355No Hit
GGTACAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5210.19772296015180263No Hit
GGTACAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG5190.1969639468690702No Hit
GGTACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA5030.19089184060721062No Hit
GGTACAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT4930.1870967741935484No Hit
GGTACAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC4920.18671726755218218No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC4910.18633776091081594No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC4820.18292220113851992No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC4660.17685009487666034No Hit
GGTACAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC4630.17571157495256168No Hit
GGTACAGGGGGTGAAGACCACAGTTTCCTGTCGCGTGGATGTGTTTCCTCTC4570.17343453510436432No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCC4510.17115749525616697No Hit
GGTACAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG4370.16584440227703984No Hit
GGTACAGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGG4360.16546489563567363No Hit
GGTACAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG4280.16242884250474385No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC4250.16129032258064516No Hit
GGTACAGGGGGGGATGAGAAGAGATGGCTCAGCAGTTAGAAGCAGTGACTAC4210.15977229601518025No Hit
GGTACAGGGGCGTTCCCAGGGCCTCATGGAGATTATAATGTCAGCAAAACCC4090.15521821631878557No Hit
GGTACAGGGAGCACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAG4090.15521821631878557No Hit
GGTACAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG3990.15142314990512334No Hit
GGTACAGGGGGTTTTCCGCAGCCTTGCTGCCTTCTCTCTGAACGCGGCTTCC3940.14952561669829223No Hit
GGTACAGGGGGCCGGCGCGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCC3880.14724857685009488No Hit
GGTACAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA3870.14686907020872864No Hit
GGTACAGGGATCTACATCGAACGAGTCCAGCGAGAGAAGGCTAATGGCACAA3830.14535104364326376No Hit
GGTACAGGGGGTTTGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCG3770.1430740037950664No Hit
GGTACAGGGGGAGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGC3770.1430740037950664No Hit
GGTACAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC3640.1381404174573055No Hit
GGTACAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC3470.1316888045540797No Hit
GGTACAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC3400.12903225806451613No Hit
GGTACAGGGGTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGTGA3400.12903225806451613No Hit
GGTACAGGGGACTGTCGTCCTGCGGCCGTGCCGCTTCCAGTCTCCAGACCAT3370.12789373814041743No Hit
GGTACAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT3350.127134724857685No Hit
GGTACAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG3310.1256166982922201No Hit
GGTACAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATA3250.12333965844402277No Hit
GGTACAGGGGGTAAAGGGCTGGGGCTCAGCCCGGCAGGTTTAAAGGGTTGGG3250.12333965844402277No Hit
GGTACAGGGGGTCAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGG3240.12296015180265656No Hit
GGTACAGGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA3230.12258064516129032No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC3200.12144212523719165No Hit
GGTACAGGGGCTGTTATCCTAGGCCTCTAGACTGCAAGAGTACTGGCCCTAA3200.12144212523719165No Hit
GGTACAGGGGACAAGAGGCCCAGTACTTCCTCTGCCCTGCTGCAGGGGCCAG3170.12030360531309298No Hit
GGTACAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGAGG3090.11726755218216318No Hit
GGTACAGGGGTTTCTCTCTGAGCAATGTCTGGACCCTGAGCTCCCTTTGCAG2950.11195445920303607No Hit
GGTACAGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC2910.11043643263757116No Hit
GGTACAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA2880.10929791271347249No Hit
GGTACAGGGGGCTGGAGGACTCTTAACTGCCGCCGCGATGCCGAAGGGTCCC2870.10891840607210627No Hit
GGTACAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA2830.10740037950664136No Hit
GGTACAGGGGGAGTTCGGCAGTGAGGCAACATGTCGTCGCCGACGATCCCTT2830.10740037950664136No Hit
GGTACAGGGGTACAGGGGGCATCTAATACAACAGTTAGGGAAGGATGAATTA2820.10702087286527515No Hit
GGTACAGGGGGTTGAAGAAGACCGAGAGTTCCTCGGGGACTCGCTCAGCTCC2810.1066413662239089No Hit
GGTACAGGGGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCT2810.1066413662239089No Hit
GGTACAGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC2800.1062618595825427No Hit
GGTACAGGGGAGCCGCTCCAAGATGGCGACTCCCATGCATCGGCTCATAGCT2790.10588235294117646No Hit
GGTACAAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA2790.10588235294117646No Hit
GGTACAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC2760.1047438330170778No Hit
GGTACAGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG2760.1047438330170778No Hit
GGTACAGGGGTTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGA2740.10398481973434535No Hit
GGTACAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT2700.10246679316888047No Hit
GGTACAGGGGTGTGTGTGTATATATGAGCAGAGTTTGGGGGTTTGTTTTGGG2670.10132827324478177No Hit
GGTACAGGGGAGGCTGGGCAATGGTGGCACATGCCTTTGATCCCAGCACTTG2660.10094876660341556No Hit
GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT2660.10094876660341556No Hit
GGTACAGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC2650.10056925996204934No Hit
GGTACAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2650.10056925996204934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA351.01556E-746.00000431
ATAGGTC351.01556E-746.00000435
CTATAGC351.01556E-746.00000433
GTTAGGA351.01556E-746.00000434
AATATTA351.01556E-746.00000416
ACCTAAA351.01556E-746.00000421
TCTACGC351.01556E-746.00000413
CTACGCT351.01556E-746.00000414
GTCAAAT351.01556E-746.00000439
ACTAACC351.01556E-746.00000417
CTCGACA351.01556E-746.00000445
TATAGCC351.01556E-746.00000434
AACCTAA351.01556E-746.00000420
CGCAACC351.01556E-746.00000414
CCTATAG351.01556E-746.00000432
GGTTAGG351.01556E-746.00000433
CAATAAT351.01556E-746.00000412
CCTGACG551.8189894E-1246.00000432
TACACTA351.01556E-746.00000414
AATCGTA351.01556E-746.00000420
Produced by FastQC (version 0.11.5)