FastQCFastQC Report
Fri 17 Jun 2016
SRR1527442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284078
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC79972.815071916867902No Hit
GGTACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG33141.166581009441069No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT26680.9391786762790502No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT23270.8191412217771175No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC14750.5192235935200896No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC13680.48155788199015764No Hit
GGTACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT12620.4442441864558325No Hit
GGTACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11990.42206717873260163No Hit
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10330.3636325234618661No Hit
GGTACAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG10250.3608163954970114No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT9860.3470877716683446No Hit
GGTACAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9380.33019100387921624No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT8580.30202972423066904No Hit
GGTACAGGGGAGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGCT7470.2629559487183098No Hit
GGTACAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA6410.2256422531839847No Hit
GGTACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG6290.2214180612367026No Hit
GGTACAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC6170.2171938692894205No Hit
GGTACAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG6070.2136737093333521No Hit
GGTACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA5860.20628137342560846No Hit
GGTACAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5790.20381726145636056No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC5740.2020571814783264No Hit
GGTACAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5540.19501686156618958No Hit
GGTACAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG5480.19290476559254852No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC5350.1883285576496596No Hit
GGTACAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT5330.18762452565844592No Hit
GGTACAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG5300.1865684776716254No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC5220.18375234970677068No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC5190.18269630171995016No Hit
GGTACAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG5100.17952815775948858No Hit
GGTACAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG4930.1735438858341723No Hit
GGTACAGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGG4770.16791162990446284No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCC4680.1647434859440013No Hit
GGTACAGGGGGTGAAGACCACAGTTTCCTGTCGCGTGGATGTGTTTCCTCTC4520.15911123001429187No Hit
GGTACAGGGGCGTTCCCAGGGCCTCATGGAGATTATAATGTCAGCAAAACCC4500.15840719802307818No Hit
GGTACAGGGAGCACTGTCCCTCAGCACTGCTCCTCTCTTGCTGATCTTTTAG4370.15383099008018924No Hit
GGTACAGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG4260.149958814128514No Hit
GGTACAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC4240.1492547821373003No Hit
GGTACAGGGGACTGTCGTCCTGCGGCCGTGCCGCTTCCAGTCTCCAGACCAT4150.14608663817683876No Hit
GGTACAGGGATCTACATCGAACGAGTCCAGCGAGAGAAGGCTAATGGCACAA4130.14538260618562507No Hit
GGTACAGGGGGTTTTCCGCAGCCTTGCTGCCTTCTCTCTGAACGCGGCTTCC4110.14467857419441138No Hit
GGTACAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA4090.14397454220319772No Hit
GGTACAGGGGGTTTGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCG3980.14010236625152248No Hit
GGTACAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC3890.1369342222910609No Hit
GGTACAGGGGGAGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGC3860.13587817430424037No Hit
GGTACAGGGGGGGATGAGAAGAGATGGCTCAGCAGTTAGAAGCAGTGACTAC3810.13411809432620617No Hit
GGTACAGGGGCTGTTATCCTAGGCCTCTAGACTGCAAGAGTACTGGCCCTAA3710.13059793437013778No Hit
GGTACAGGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC3700.13024591837453092No Hit
GGTACAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT3670.12918987038771043No Hit
GGTACAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC3620.1274297904096762No Hit
GGTACAGGGGTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGTGA3530.12426164644921465No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC3480.12250156647118046No Hit
GGTACAGGGGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATA3410.12003745450193257No Hit
GGTACAGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC3370.1186293905195052No Hit
GGTACAGGGGGTCAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGG3330.11722132653707784No Hit
GGTACAGGGGTTTCTCTCTGAGCAATGTCTGGACCCTGAGCTCCCTTTGCAG3310.11651729454586417No Hit
GGTACAGGGAGCGTGTGGAAAATACTACAGAGTATGCACACTGGCTATCATT3290.11581326255465049No Hit
GGTACAGGGGGAGTTCGGCAGTGAGGCAACATGTCGTCGCCGACGATCCCTT3290.11581326255465049No Hit
GGTACAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGAGG3280.11546124655904363No Hit
GGTACAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG3280.11546124655904363No Hit
GGTACAGGGGAGCCGCTCCAAGATGGCGACTCCCATGCATCGGCTCATAGCT3270.11510923056343682No Hit
GGTACAGGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA3260.11475721456782996No Hit
GGTACAGGGGTTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGA3230.11370116658100944No Hit
GGTACAGGGGGTAAAGGGCTGGGGCTCAGCCCGGCAGGTTTAAAGGGTTGGG3220.1133491505854026No Hit
GGTACAGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG3220.1133491505854026No Hit
GGTACAGGGGGTTGAAGAAGACCGAGAGTTCCTCGGGGACTCGCTCAGCTCC3180.11194108660297523No Hit
GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3180.11194108660297523No Hit
GGTACAGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC3110.10947697463372737No Hit
GGTACAGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTC3070.10806891065129999No Hit
GGTACAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG3020.1063088306732658No Hit
GGTACAAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA2990.10525278268644526No Hit
GGTACAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA2970.10454875069523159No Hit
GGTACAGGGATCGGAGGCTTATTTTTCCGCGAGCTGCCGTTCCACGGACGCA2970.10454875069523159No Hit
GGTACAGGGGACAAGAGGCCCAGTACTTCCTCTGCCCTGCTGCAGGGGCCAG2950.10384471870401792No Hit
GGTACAGGGGTGTGTGTGTATATATGAGCAGAGTTTGGGGGTTTGTTTTGGG2930.10314068671280423No Hit
GGTACAGGGGTGGCTGAGAAAACCAAGGAGCAGGCATCGCATCTGGGAGGAG2920.1027886707171974No Hit
GGTACAGGGGCCATCAGCCTGCTGCCTATCTCCTATAGCCATTGCTGGCCAC2910.10243665472159055No Hit
GGTACAGGGAGTATTTGTGTATGGAATCTGTGCATTATCATATTTTTTCAGC2910.10243665472159055No Hit
GGTACAGGGGTCTTACTGCGTCCCACAATATCTGAATGTCTCAACCCTCCCT2890.10173262273037688No Hit
GGTACAGGGGGCCGGCGCGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCC2850.1003245587479495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAATA351.01594196E-746.00000435
ACGGGTA351.01594196E-746.00000430
TTATCAG700.046.00000412
ATCGTCC351.01594196E-746.00000430
CTCATTA700.046.0000048
TCGTCCA351.01594196E-746.00000431
TATTAGT351.01594196E-746.00000433
CGAATCG351.01594196E-746.00000427
ATAAGCA351.01594196E-746.00000441
ACTAAGT351.01594196E-746.00000443
GTATCAC351.01594196E-746.00000414
TGGATAC351.01594196E-746.00000413
GGTCTAA351.01594196E-746.00000425
TCGAATC351.01594196E-746.00000426
ATACCGC351.01594196E-746.00000416
TCAACGG351.01594196E-746.00000427
AATAAGC351.01594196E-746.00000440
TCACGCG253.4106168E-546.026
ATTTACT206.303393E-446.035
AACGTTC206.303393E-446.040
Produced by FastQC (version 0.11.5)