FastQCFastQC Report
Fri 17 Jun 2016
SRR1527441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92877
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG23742.5560687791379997Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA12931.3921638295810588Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA9851.0605424378479065Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA9020.9711769329328036Illumina Single End Adapter 2 (96% over 26bp)
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8560.9216490627388912No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGA7990.860277571411652No Hit
CATCCCGGGATGAGCACTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG7890.8495106431086275Illumina Single End Adapter 2 (100% over 30bp)
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCT6750.7267676604541491Illumina Single End Adapter 2 (96% over 29bp)
CATCCCGGGATGAGCACTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA5210.5609569645875728Illumina Single End Adapter 1 (100% over 25bp)
CATCCCGGGATGAGCACTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT4070.43821398193309424Illumina Single End Adapter 2 (96% over 33bp)
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG3930.42314028230886014No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT3440.3703823336240404No Hit
CATCCCGGGGAGGCGTCATTCCCTTAACTTAGACCATTTCCTTCCAAGCA3410.3671522551331331No Hit
CATCCCGGGATGAGCACTGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3270.3520785555088988TruSeq Adapter, Index 23 (96% over 25bp)
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT3120.3359281630543622No Hit
CATCCCGGGATGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA3070.33054469890285004Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGATGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA2960.3187010777695231Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2720.2928604498422645No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC2650.2853236000301474No Hit
CATCCCGGGCACCTCTCCTTGCCAAATAGTCCGTGCCTTGCCTTGGAGGG2230.24010250115744478No Hit
CATCCCGGGCACTTCCTCATATTTCTGTACCAGTGACCTCCTGCCCAGAA2110.22718218719381547No Hit
CATCCCGGGATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA2080.22395210870290813Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2060.22179872304230325Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACT1970.21210848756958128No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1720.1851911668120202No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA1690.18196108832111288Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCACTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC1660.17873100983020554Illumina Single End Adapter 2 (96% over 31bp)
CATCCCGGGAACTTCTGTGCCTTGGATCTGCCCAGCTGCAATGTTGGAAG1620.17442423850899577No Hit
CATCCCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT1620.17442423850899577No Hit
CATCCCGGGATGAGCACATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA1610.17334754567869334Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT1550.16688738869687866No Hit
CATCCCGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC1530.16473400303627378No Hit
CATCCCGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1500.16150392454536644Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGCGA1480.15935053888476156No Hit
CATCCCGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT1460.15719715322415667No Hit
CATCCCGGGATGACTAAGGACCAAAGAAAGCCCCCAAGCAGATGTCTGTG1440.15504376756355182No Hit
CATCCCGGGAACAGTCCTTTGACTAGTTTGCTGCCTGAATAATATTTACA1430.15396707473324936No Hit
CATCCCGGGATGAGCACTAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA1400.15073699624234202Illumina Single End Adapter 2 (96% over 27bp)
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC1390.14966030341203956No Hit
CATCCCGGGGGCTTGCCAGTGAGCTTCCTCTCCGGGACCACTGCAGCAGG1360.14643022492113225No Hit
CATCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG1310.14104676076962003No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC1290.13889337510901514No Hit
CATCCCGGGGTCTCCTGAGGTCTGGCTCACCCCTGCAAATGTCACTGGCA1240.13350991095750295No Hit
CATCCCGGGCTTTCAAGGTACTGTACATTGTTGACGGGATGCCAAGCGGG1240.13350991095750295No Hit
CATCCCGGGGTCCTAGATTGACTTACACACATAGCCTAGAGCTCAGTTTT1200.12920313963629315No Hit
CATCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCTACGGC1200.12920313963629315No Hit
CATCCCGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC1170.12597306114538584No Hit
CATCCCGGGAGCTAGGTTGGGGTCGTTTTAGAATGTTTCCCTAGTCATCT1160.1248963683150834No Hit
CATCCCGGGAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTTT1100.11843621133326875No Hit
CATCCCGGGGGTCAGTGGAAATGAAAATAAAAAAAAATAAAAAATAAAAA1100.11843621133326875No Hit
CATCCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC1060.11412944001205896No Hit
CATCCCGGGAGTCATATCTAATGTAGAAAAATTAGTTTCCAGTGAAAGTA1030.11089936152115162No Hit
CATCCCGGGATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA1020.10982266869084918Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGTAAACTGCTATGCATAGCTTGGGCAGTAGCCCCAAACT1020.10982266869084918No Hit
CATCCCGGGGTGCTGCCCGTAGCCACACAGATCCAACAAGAGGTTATTAA1020.10982266869084918No Hit
CATCCCGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAA1010.10874597586054675No Hit
CATCCCAGGACCAACGTTCCCAGGGACTCTAAAATCTCAGGGACAGTTGA1010.10874597586054675No Hit
CATCCCGGGACCCAAAGCCTTGGCTATTCTCAGTGTGGGGGAGGGGGACA1010.10874597586054675No Hit
CATCCCGGGATTATAATTGTGCGTTTTACATTTTAAAAGTAAGTAAACTT1000.10766928303024431No Hit
CATCCCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA1000.10766928303024431No Hit
CATCCCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG990.10659259019994187No Hit
CATCCCGGGGGGCTGGCGAGAGGGCTCAGCGGGTAAGAGCACTGACTGCT980.10551589736963941No Hit
CATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATT980.10551589736963941No Hit
CATCCCGGGATGTATTTTCTATCTTTATTTTTTTACTGGGCATTTCTCAG980.10551589736963941No Hit
CATCCCGGGAAGATGATCTGCCGCAAGTGCTACGCACGCCTGCACCCTCG980.10551589736963941No Hit
CATCCCGGGGAGATGGTGCTGAACTGGAACCCAGGTATAAATGAAGGTTG970.10443920453933697No Hit
CATCCCGGGATGAGCACTGCACTGATCGGAAGAGCTCGTATGCCGTCTTC960.10336251170903453Illumina Single End Adapter 2 (96% over 28bp)
CATCCCGGGATGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA960.10336251170903453Illumina Single End Adapter 1 (96% over 29bp)
CATCCCGGAGTCACGAGTCATCTCCAGGACGCTACGCTGCTTCGAATCCT950.10228581887873209No Hit
CATCCCGGGATGTCCGAGCTGGAGAAGGCCATGGTTGCCCTCATTGATGT950.10228581887873209No Hit
CATCCCGGGATGAGCACTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCT950.10228581887873209Illumina Single End Adapter 2 (96% over 32bp)
CATCCCGGGATCTGAGGAAGGTAGCACCTTCATTGGGAGGCCATCCCTGG930.10013243321812719No Hit
CATCCCGGGATGTGGCTCAGCTCAAGGCATTCATTTCATTCTAATCTTTA930.10013243321812719No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG930.10013243321812719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGAT551.8189894E-1244.00000431
CTAGATA600.044.019
CTAGACT254.4112017E-544.023
AAGTACG207.8191055E-444.023
CTCGTTC207.8191055E-444.044
GATGTAT207.8191055E-444.09
TTGTGCG207.8191055E-444.017
AGGAGTG207.8191055E-444.016
GGATGTG1650.044.08
GGATGTA750.044.08
GAATGGA254.4112017E-544.09
CAACGCG207.8191055E-444.022
GGATGCA1000.044.08
ATGGCCA207.8191055E-444.010
TAGACCA454.6929927E-1044.030
CATGATT502.7284841E-1144.032
TTTCGGT207.8191055E-444.027
CACATCG254.4112017E-544.015
TATATGG207.8191055E-444.038
CTAAGTG207.8191055E-444.015
Produced by FastQC (version 0.11.5)