FastQCFastQC Report
Fri 17 Jun 2016
SRR1527440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110077
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA30052.72990724674546Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA15891.4435349800594128Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15231.3835769506799787Illumina Single End Adapter 2 (96% over 26bp)
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA14941.3572317559526514Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCACTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT12621.1464701981340335Illumina Single End Adapter 2 (100% over 32bp)
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC12141.102864358585354Illumina Single End Adapter 2 (96% over 31bp)
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC9390.8530392361710439No Hit
CATCCCGGGATGAGCACTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7790.7076864376754454Illumina Single End Adapter 1 (100% over 25bp)
CATCCCGGGATGAGCACTGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6620.601397203775539TruSeq Adapter, Index 23 (96% over 25bp)
CATCCCGGGATGAGCACTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA5640.5123686146969848Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGAAG5550.5041925197816074No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4720.42879075556201574No Hit
CATCCCGGGGAGGCGTCATTCCCTTAACTTAGACCATTTCCTTCCAAGCAGA4150.3770088210979587No Hit
CATCCCGGGATGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA3910.3552059013236189Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA3780.34339598644585156Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT3710.33703680151166915No Hit
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3580.3252268866339017Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC3430.3116000617749394No Hit
CATCCCGGGATGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3370.3061493318313544Illumina Single End Adapter 1 (96% over 29bp)
CATCCCGGGATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3320.301607056878367Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT3200.2907055969911971No Hit
CATCCCGGGATGAGCACATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA2740.2489166674237125Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA2650.24074057250833505No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCA2580.23438138757415264No Hit
CATCCCGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2410.2189376527339953Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGATGAGCACTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT2350.21348692279041034Illumina Single End Adapter 2 (96% over 33bp)
CATCCCGGGCACTTCCTCATATTTCTGTACCAGTGACCTCCTGCCCAGAACC2270.2062192828656304No Hit
CATCCCGGGCACCTCTCCTTGCCAAATAGTCCGTGCCTTGCCTTGGAGGGTT2180.19804318795025302No Hit
CATCCCGGGAACTTCTGTGCCTTGGATCTGCCCAGCTGCAATGTTGGAAGCC2070.18805018305368063No Hit
CATCCCGGGATGAGCACTAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2060.1871417280630831Illumina Single End Adapter 2 (96% over 27bp)
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA2010.18259945311009568Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTT1910.17351490320412075No Hit
CATCCCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT1850.1680641732605358No Hit
CATCCCGGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA1840.1671557182699383Illumina Single End Adapter 2 (96% over 30bp)
CATCCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT1790.16261344331695085No Hit
CATCCCGGGATGAGCACTGCACTCGATCGGAAGAGCTCGTATGCCGTCTTCT1770.1607965333357559Illumina Single End Adapter 2 (96% over 29bp)
CATCCCGGGAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTTTGG1710.15534580339217094No Hit
CATCCCGGGATGACTAAGGACCAAAGAAAGCCCCCAAGCAGATGTCTGTGGT1700.15443734840157344No Hit
CATCCCGGGATGAGCACTGCACTGATCGGAAGAGCTCGTATGCCGTCTTCTG1670.15171198342978098Illumina Single End Adapter 2 (96% over 30bp)
CATCCCGGGGTCCTAGATTGACTTACACACATAGCCTAGAGCTCAGTTTTAG1650.149895073448586No Hit
CATCCCGGGGGCTTGCCAGTGAGCTTCCTCTCCGGGACCACTGCAGCAGGCT1650.149895073448586No Hit
CATCCCGGGAAGGTGTTGGGAAAGACCCCTGTGATCAGAGAGTGTCAACTTA1630.148078163467391No Hit
CATCCCGGGCTTTCAAGGTACTGTACATTGTTGACGGGATGCCAAGCGGGCT1600.14535279849559854No Hit
CATCCCGGGAACAGTCCTTTGACTAGTTTGCTGCCTGAATAATATTTACAAA1600.14535279849559854No Hit
CATCCCGGGATGAGCATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA1530.1389936135614161Illumina Single End Adapter 1 (96% over 33bp)
CATCCCGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCCG1510.1371767035802211No Hit
CATCCCGGGGTGCTGCCCGTAGCCACACAGATCCAACAAGAGGTTATTAAAG1490.13535979359902614No Hit
CATCCCGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTT1480.13445133860842864No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATG1480.13445133860842864No Hit
CATCCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT1460.13263442862723368No Hit
CATCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCTACGGCTG1450.13172597363663616No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC1410.1280921536742462No Hit
CATCCCGGGACCCAAAGCCTTGGCTATTCTCAGTGTGGGGGAGGGGGACAGG1370.12445833371185624No Hit
CATCCCGGGAGCTAGGTTGGGGTCGTTTTAGAATGTTTCCCTAGTCATCTTA1370.12445833371185624No Hit
CATCCCGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT1370.12445833371185624No Hit
CATCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG1360.12354987872125875No Hit
CATCCCGGGGTCAGATGATTTAATCTATTTAAGTGTTGATCTGCTAGCAGAA1360.12354987872125875No Hit
CATCCCGGGGTCTCCTGAGGTCTGGCTCACCCCTGCAAATGTCACTGGCAAC1340.12173296874006378No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC1330.12082451374946628No Hit
CATCCCGGGATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1330.12082451374946628Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGAGTCATATCTAATGTAGAAAAATTAGTTTCCAGTGAAAGTAAT1310.11900760376827131No Hit
CATCCCGGGATGAGCACTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1290.11719069378707632Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTA1270.11537378380588134No Hit
CATCCCGGGGGTCAGTGGAAATGAAAATAAAAAAAAATAAAAAATAAAAAAA1250.11355687382468636No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAA1230.11173996384349137Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGAGATGGTGCTGAACTGGAACCCAGGTATAAATGAAGGTTGGT1230.11173996384349137No Hit
CATCCCGGGGGAGAGATGGCTCAGTGGTTAAGAGCACGGACTGCTCTTCCAA1210.10992305386229641No Hit
CATCCCGGGATGTAAACTGCTATGCATAGCTTGGGCAGTAGCCCCAAACTGC1210.10992305386229641No Hit
CATCCCGGGGGGCTGGCGAGAGGGCTCAGCGGGTAAGAGCACTGACTGCTCT1200.1090145988716989No Hit
CATCCCGGGATGGACGTGTTTCTCATGATCCGGCGCCACAAGACCACCATCT1190.10810614388110142No Hit
CATCCCGGGAAGATGATCTGCCGCAAGTGCTACGCACGCCTGCACCCTCGTG1190.10810614388110142No Hit
CATCCCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC1190.10810614388110142No Hit
CATCCCGGGGCAGGTGGCATGCCTGGGGGAATGCCTGGTGGCTTCCCAGGTG1180.10719768889050393No Hit
CATCCCGGGGTTATAAACAGCTTTATAATATTGAAAACTTAGTGCTTAATTC1180.10719768889050393No Hit
CATCCCAGGACCAACGTTCCCAGGGACTCTAAAATCTCAGGGACAGTTGAGG1130.10265541393751647No Hit
CATCCCGGGATGTATTTTCTATCTTTATTTTTTTACTGGGCATTTCTCAGTG1130.10265541393751647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCC551.8189894E-1246.00000417
ACGCGAG206.286485E-446.024
CTTAATT253.3969154E-546.045
GTCATTC501.6370905E-1146.015
GTTGACG206.286485E-446.030
CTAGATT253.3969154E-546.013
TATTTAC206.286485E-446.043
GAGTGTC253.3969154E-546.040
AACCGGC253.3969154E-546.028
CTCACTA301.8459959E-646.045
ACTGCGC206.286485E-446.016
ATTAGTT253.3969154E-546.031
ATGATCG253.3969154E-546.010
CTCAACG206.286485E-446.034
CAACGTG206.286485E-446.036
TACCAGT301.8459959E-646.028
CAACGCG206.286485E-446.022
GGATGCG206.286485E-446.08
ACCATAG301.8459959E-646.034
ATGGAAT206.286485E-446.010
Produced by FastQC (version 0.11.5)