FastQCFastQC Report
Fri 17 Jun 2016
SRR1527439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86305
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA11581.3417530849892823Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGAAG7890.9141996408087596No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7880.9130409593882162No Hit
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA7270.8423613927350675Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA6350.7357627020450728Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5660.6558136840275767Illumina Single End Adapter 2 (96% over 26bp)
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT3990.4623138867968253No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT3620.4194426742367186No Hit
CATCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT3490.4043798157696541No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC3080.3568738775273739No Hit
CATCCCGGGGAGGCGTCATTCCCTTAACTTAGACCATTTCCTTCCAAGCAGA3060.354556514686287No Hit
CATCCCGGGATGAGCACTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3010.3487631075835699Illumina Single End Adapter 1 (100% over 25bp)
CATCCCGGGATGAGCACTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT2840.3290655234343317Illumina Single End Adapter 2 (100% over 32bp)
CATCCCGGGCACTTCCTCATATTTCTGTACCAGTGACCTCCTGCCCAGAACC2630.3047332136029199No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA2610.302415850761833No Hit
CATCCCGGGATGAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA2350.27229013382770406Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC2220.2572272753606396Illumina Single End Adapter 2 (96% over 31bp)
CATCCCGGGATGAGCACTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA2170.2514338682579225Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGCACCTCTCCTTGCCAAATAGTCCGTGCCTTGCCTTGGAGGGTT2150.24911650541683564No Hit
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA2110.24448177973466195Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA2080.2410057354730317Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCA1970.22826023984705404No Hit
CATCCCGGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTT1940.22478419558542378No Hit
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1910.22130815132379353Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1820.2108800185389027Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT1710.1981345229129251No Hit
CATCCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT1630.1888650715485777No Hit
CATCCCGGGGTCTCCTGAGGTCTGGCTCACCCCTGCAAATGTCACTGGCAAC1630.1888650715485777No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC1610.18654770870749088No Hit
CATCCCGGGGGCTTGCCAGTGAGCTTCCTCTCCGGGACCACTGCAGCAGGCT1590.18423034586640402No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC1570.1819129830253172No Hit
CATCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG1460.16916748739933954No Hit
CATCCCGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTT1440.1668501245582527No Hit
CATCCCGGGGTCCTAGATTGACTTACACACATAGCCTAGAGCTCAGTTTTAG1440.1668501245582527No Hit
CATCCCGGGAACTTCTGTGCCTTGGATCTGCCCAGCTGCAATGTTGGAAGCC1430.16569144313770928No Hit
CATCCCGGGGTGCTGCCCGTAGCCACACAGATCCAACAAGAGGTTATTAAAG1370.15873935461444877No Hit
CATCCCGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAATG1360.15758067319390534No Hit
CATCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCTACGGCTG1340.15526331035281848No Hit
CATCCCGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCCG1290.14946990325010137No Hit
CATCCCGGGATGTATTTTCTATCTTTATTTTTTTACTGGGCATTTCTCAGTG1240.14367649614738426No Hit
CATCCCGGGCTTTCAAGGTACTGTACATTGTTGACGGGATGCCAAGCGGGCT1240.14367649614738426No Hit
CATCCCGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1220.14135913330629743Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGAGCTAGGTTGGGGTCGTTTTAGAATGTTTCCCTAGTCATCTTA1210.140200451885754No Hit
CATCCCGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT1200.1390417704652106No Hit
CATCCCGGGGGAGAGATGGCTCAGTGGTTAAGAGCACGGACTGCTCTTCCAA1160.13440704478303692No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATG1160.13440704478303692No Hit
CATCCCGGGGGTCAGTGGAAATGAAAATAAAAAAAAATAAAAAATAAAAAAA1150.1332483633624935No Hit
CATCCCGGGATGAGCACTGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1130.13093100052140663TruSeq Adapter, Index 23 (96% over 25bp)
CATCCCGGGATGAGCACTAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1100.12745495625977638Illumina Single End Adapter 2 (96% over 27bp)
CATCCCAGGACCAACGTTCCCAGGGACTCTAAAATCTCAGGGACAGTTGAGG1070.1239789119981461No Hit
CATCCCGGGATGACTAAGGACCAAAGAAAGCCCCCAAGCAGATGTCTGTGGT1060.12282023057760269No Hit
CATCCCGGGGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAAT1060.12282023057760269No Hit
CATCCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT1060.12282023057760269No Hit
CATCCCGGGAAGGTTTTTTGTACTCAATTCTTTAAGAAATGACAAATTTTGG1050.12166154915705928No Hit
CATCCCGGGACCACCTGTAGCTCTTGTTGACCTGCCTGTTCACCTCATGAGT1050.12166154915705928No Hit
CATCCCGGGGGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCGCGCCGTCA1050.12166154915705928No Hit
CATCCCGGGACCCAAAGCCTTGGCTATTCTCAGTGTGGGGGAGGGGGACAGG1030.11934418631597242No Hit
CATCCCGGGAAGCTTAAGGCGGCCTACAGAAGAAAAAGAAAAAAAAGGCCAC1020.11818550489542899No Hit
CATCCCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTAT1010.11702682347488559No Hit
CATCCCGGGGAGATGGTGCTGAACTGGAACCCAGGTATAAATGAAGGTTGGT1010.11702682347488559No Hit
CATCCCGGGAGTCATATCTAATGTAGAAAAATTAGTTTCCAGTGAAAGTAAT1010.11702682347488559No Hit
CATCCCGGGAAGATGATCTGCCGCAAGTGCTACGCACGCCTGCACCCTCGTG1000.11586814205434216No Hit
CATCCCGGGAAGAAGAGAAAGGGCCAAGTGATCCAGTTCTAAGCAGATTTTG1000.11586814205434216No Hit
CATCCCGGGATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1000.11586814205434216Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGAACTGACTGCT1000.11586814205434216No Hit
CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG990.11470946063379873No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGGAG990.11470946063379873No Hit
CATCCCGGAGTCACGAGTCATCTCCAGGACGCTACGCTGCTTCGAATCCTAA990.11470946063379873No Hit
CATCCCGGGGTTATAAACAGCTTTATAATATTGAAAACTTAGTGCTTAATTC990.11470946063379873No Hit
CATCCCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC980.11355077921325532No Hit
CATCCCGGGAACAGTCCTTTGACTAGTTTGCTGCCTGAATAATATTTACAAA980.11355077921325532No Hit
CATCCCGGGAGTTAAAGGAGTGTTTGGTAGAGGTATATGTATATATACCTCA950.11007473495162506No Hit
CATCCCGGGGGGCTGGCGAGAGGGCTCAGCGGGTAAGAGCACTGACTGCTCT950.11007473495162506No Hit
CATCCCGGGGCAGGTGGCATGCCTGGGGGAATGCCTGGTGGCTTCCCAGGTG940.10891605353108164No Hit
CATCCCGGGATTGTAAAACCAAGGACAATTTTATAACTTTTTTGTACGTAGC940.10891605353108164No Hit
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAA930.10775737211053821Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGGAATCATTTATGGGCTCCTTTAATGGGCCCATAAAAGCTCTTA920.10659869068999478No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAAA920.10659869068999478Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGGACGTGTTTCTCATGATCCGGCGCCACAAGACCACCATCT910.10544000926945135No Hit
CATCCCGGGATAGTTCTGGATTATGTTGAAAGTGTTAGATGGCTAAGTATTA910.10544000926945135No Hit
CATCCCGGGATGAGCACATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA910.10544000926945135Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGTCAAACAGTCTCAAGGTTGCAGTCGTACTCTATAAAGTGA910.10544000926945135No Hit
CATCCCGGGGGGCTGGCGAGGTGGCTCAGTGGGTAAGAGCACCCGACTGCTC900.10428132784890795No Hit
CATCCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC890.10312264642836452No Hit
CATCCCGGGCTACCGTCTGTTCCGGGACGCCACCAGATATATGGAGGGACAT880.1019639650078211No Hit
CATCCCGGGATGTGGCTCAGCTCAAGGCATTCATTTCATTCTAATCTTTACC880.1019639650078211No Hit
CATCCCGGGCTGTGAGAGAGGCTACCATGACTTCAGCGTGAAACTCTTGGCC880.1019639650078211No Hit
CATCCCGGGGTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG870.10080528358727768No Hit
CATCCCGGGATTATAATTGTGCGTTTTACATTTTAAAAGTAAGTAAACTTTG870.10080528358727768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAA206.2788994E-446.010
CTAGATA600.046.019
CTAGACT301.8413248E-646.011
AGTCCGT206.2788994E-446.028
GGAGCTA301.8413248E-646.08
AGTCCAC206.2788994E-446.011
AGATCCA206.2788994E-446.029
TCACCTC206.2788994E-446.040
GATGTAT206.2788994E-446.09
GAGCTAG253.390773E-546.09
CGAGTGG301.8413248E-646.015
CGAGTCG253.390773E-546.028
CCTAGTC206.2788994E-446.040
CACGGAC206.2788994E-446.035
CCCTAGT206.2788994E-446.039
GAGTCCG301.8413248E-646.010
GCGCGAA301.8413248E-646.020
TAGGTTG206.2788994E-446.013
GAACTTC253.390773E-546.09
TAGAATG206.2788994E-446.029
Produced by FastQC (version 0.11.5)