SRR1527438_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	525285
Sequences flagged as poor quality	0
Sequence length	50
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2801	0.5332343394538203	No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2408	0.45841781128339854	No Hit
ACCTGAGGGAGTCCTAGCCACATTATTGACAGTCTTGCATTTTGTTTTGT	1632	0.31068848339472854	No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1575	0.2998372312173392	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1185	0.22559182158256946	No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1140	0.21702504354778832	No Hit
ACCTGAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT	1042	0.19836850471648726	No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1001	0.19056321806257553	No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	938	0.17856972881388197	No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	907	0.17266817061214387	No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	859	0.1635302740417107	No Hit
ACCTGAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG	827	0.15743834299475523	No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	820	0.15610573307823372	No Hit
ACCTGAGGGACTGAGATTCTGTTAGGGGGCGTTTACATTGAGAAGAATGA	797	0.15172715763823447	No Hit
ACCTGAGGGGGGGTTTTCTCAGAGTCCATTGCCGGCAATGGGCAAGCCTG	774	0.14734858219823524	No Hit
ACCTGAGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	771	0.14677746366258318	No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	749	0.1425892610678013	No Hit
ACCTGAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	676	0.12869204336693413	No Hit
ACCTGAGGGAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAAC	670	0.12754980629562998	No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	650	0.12374234939128283	No Hit
ACCTGAGGGACCACTGTCAGTTGCAGGTTTCATTTCATTCTCATGGCTAT	641	0.1220289937843266	No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	640	0.12183862093910924	No Hit
ACCTGAGGGGTGGAGTGGATTCTGAAGAGCCCCTTTAGACTAGAGTGTTG	639	0.1216482480938919	No Hit
ACCTGAGGGATGGTGGCATATGCCTTTTTCTCCAGGACTCCGGAGGCAGA	630	0.11993489248693567	No Hit
ACCTGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	618	0.11765041834432736	No Hit
ACCTGAGGCACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCG	613	0.11669855411824057	No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	591	0.11251035152345869	No Hit
ACCTGAGGGACTGTTTCTCCCCAAGGGAAGAGAGGCTAAGCCAGCCACCA	591	0.11251035152345869	No Hit
ACCTGAGGGGGTTAGTTTTAGTGAAGCTTTTGCGTATTTCCTTCTATGGT	589	0.11212960583302399	No Hit
ACCTGAGGGAGGCTGTGGCTTCTTCGTTTGATCCTATAATCATACAGAGT	586	0.11155848729737192	No Hit
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	568	0.10813177608345946	No Hit
ACCTGAGGGAAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG	563	0.10717991185737266	No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	563	0.10717991185737266	No Hit
ACCTGAGGGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGC	553	0.10527618340519908	No Hit
ACCTGAGGGGGTGCCGGGATCTTCGGAGCCGCAACCGACCACACTTCCTT	530	0.10089760796519985	No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	527	0.10032648942954778	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	7.852572E-4	44.000004	30
TGATACC	20	7.852572E-4	44.000004	20
GTACCGA	20	7.852572E-4	44.000004	23
ACGCCGG	20	7.852572E-4	44.000004	27
TCACCGT	40	8.29823E-9	44.000004	37
TTAATCG	20	7.852572E-4	44.000004	25
CGAGTAT	20	7.852572E-4	44.000004	17
CGAGTAC	20	7.852572E-4	44.000004	34
ACGCATA	20	7.852572E-4	44.000004	12
CTATCGT	20	7.852572E-4	44.000004	33
GCGAAAA	20	7.852572E-4	44.000004	29
ATAATCG	20	7.852572E-4	44.000004	37
CGCATAG	20	7.852572E-4	44.000004	13
CGTCTAT	20	7.852572E-4	44.000004	17
CGATTGC	20	7.852572E-4	44.000004	43
CACGACG	20	7.852572E-4	44.000004	21
TGCGGAA	20	7.852572E-4	44.000004	38
TTATCGC	20	7.852572E-4	44.000004	39
CACCTAT	20	7.852572E-4	44.000004	33
AATGCGA	20	7.852572E-4	44.000004	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content