##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	525285
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.277544571042387	31.0	30.0	33.0	27.0	33.0
2	31.565293126588422	33.0	31.0	33.0	30.0	34.0
3	32.34160693718648	33.0	31.0	34.0	31.0	34.0
4	36.263196169698354	37.0	37.0	37.0	35.0	37.0
5	36.18918301493475	37.0	37.0	37.0	35.0	37.0
6	34.61870984322796	35.0	35.0	37.0	32.0	37.0
7	36.04441398478921	37.0	35.0	37.0	35.0	37.0
8	36.58065811892592	37.0	37.0	37.0	35.0	37.0
9	38.681685180425866	39.0	39.0	39.0	39.0	39.0
10	37.14703065954672	39.0	37.0	39.0	34.0	39.0
11	37.21128339853603	39.0	37.0	39.0	34.0	39.0
12	37.1224040282894	39.0	37.0	39.0	34.0	39.0
13	37.153134012964394	39.0	37.0	39.0	34.0	39.0
14	37.97210657071875	40.0	38.0	40.0	34.0	40.0
15	38.01433888270177	40.0	38.0	40.0	34.0	40.0
16	38.12303416240707	40.0	38.0	40.0	35.0	40.0
17	38.0451107494027	40.0	38.0	40.0	34.0	40.0
18	37.88208496340082	40.0	37.0	40.0	34.0	40.0
19	37.81435030507249	39.0	37.0	40.0	34.0	40.0
20	37.596209676651725	39.0	37.0	40.0	33.0	40.0
21	37.52458379736714	39.0	37.0	40.0	33.0	40.0
22	37.33550739122572	38.0	36.0	40.0	33.0	40.0
23	37.13541601226001	38.0	36.0	40.0	33.0	40.0
24	36.96861894019437	38.0	36.0	40.0	33.0	40.0
25	36.736445929352634	38.0	35.0	40.0	33.0	40.0
26	36.189717962629814	38.0	35.0	39.0	31.0	40.0
27	36.059520070057204	38.0	35.0	39.0	31.0	40.0
28	35.886608222203186	38.0	34.0	39.0	31.0	40.0
29	35.612680735219925	37.0	34.0	38.0	31.0	40.0
30	35.31588185461226	37.0	34.0	38.0	30.0	40.0
31	35.18509190249103	36.0	34.0	38.0	30.0	40.0
32	35.08035066678089	36.0	34.0	38.0	30.0	40.0
33	34.57053409101726	36.0	33.0	38.0	29.0	39.0
34	34.133104885919074	36.0	33.0	38.0	28.0	39.0
35	33.960579494940845	35.0	32.0	38.0	28.0	39.0
36	33.66385676347126	35.0	32.0	38.0	27.0	39.0
37	33.48349943364079	35.0	32.0	38.0	27.0	38.0
38	32.96805543657253	34.0	31.0	37.0	26.0	38.0
39	32.151721446452875	34.0	30.0	36.0	24.0	38.0
40	31.963815833309535	33.0	30.0	36.0	24.0	38.0
41	31.659403942621623	33.0	29.0	36.0	24.0	38.0
42	31.256643536365974	33.0	29.0	35.0	23.0	38.0
43	30.75428957613486	32.0	28.0	35.0	23.0	38.0
44	30.418858334047233	32.0	28.0	35.0	23.0	37.0
45	29.84364487849453	31.0	27.0	34.0	21.0	37.0
46	29.661004978249903	31.0	27.0	34.0	21.0	37.0
47	29.19442017190668	31.0	26.0	34.0	20.0	36.0
48	28.705773056531218	30.0	26.0	33.0	20.0	36.0
49	28.160689911191067	30.0	25.0	33.0	18.0	35.0
50	31.94334123380641	33.0	30.0	36.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	3.0
15	7.0
16	33.0
17	58.0
18	115.0
19	211.0
20	314.0
21	444.0
22	738.0
23	1127.0
24	1604.0
25	2414.0
26	3375.0
27	4662.0
28	6681.0
29	9309.0
30	13446.0
31	19581.0
32	31815.0
33	47903.0
34	70999.0
35	138664.0
36	148447.0
37	22850.0
38	483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.43116593849052	0.38341091026775936	0.13897217700867148	0.046450974233035396
8	99.36358357843838	0.39635626374253974	0.20883901120344195	0.031221146615646744
9	98.20478406960032	1.0198273318293878	0.22578219442778683	0.549606404142513
10	53.63317056455067	36.26298104838326	4.093396917863636	6.010451469202433
11	35.34804915426864	23.447842599731576	23.22624860789857	17.977859638101222
12	28.506049097156783	18.225725082574222	26.98896789361965	26.27925792664934
13	24.182872155115795	17.113566920814417	30.234634531730393	28.468926392339394
14	23.828969035856726	20.140304786925192	30.422342157114706	25.608384020103376
15	25.154725529950404	20.345526714069507	29.53311059710443	24.966637158875656
16	30.790903985455515	20.917597113947668	24.666419181967882	23.625079718628935
17	30.081765137020856	20.29888536699125	23.198263799651617	26.421085696336277
18	27.95929828569253	21.678707749126662	25.007376947752174	25.354617017428634
19	29.186060900273187	22.837507257964724	22.681972643422142	25.294459198339947
20	27.618530892753455	23.178084278058577	25.017847454239128	24.185537374948836
21	29.30218833585577	21.274355825884996	24.956737770924356	24.466718067334874
22	28.144150318398587	20.03750345050782	25.979039949741566	25.839306281352027
23	27.38075520907698	22.06459350638225	25.456656862465138	25.097994422075637
24	27.487173629553478	22.327117659936988	24.707349343689618	25.47835936681992
25	26.366067944068455	24.697259582893096	23.408435420771582	25.528237052266867
26	27.382658937529154	24.151079889964496	24.355921071418372	24.11034010108798
27	25.72622481129292	21.462444196959744	24.501556298009653	28.309774693737683
28	27.78339377671169	22.763071475484736	23.463072427348962	25.99046232045461
29	28.044014201814253	22.455428957613485	23.32448099603073	26.176075844541536
30	28.015648647876866	20.39064507838602	24.976155801136528	26.617550472600588
31	29.883587005149586	22.469326175314354	23.49848177655939	24.14860504297667
32	31.245323966989346	23.037589118288167	22.195379651046572	23.52170726367591
33	29.76936329801917	21.364592554518026	23.356273261182025	25.50977088628078
34	27.492884814910003	24.12423731878885	25.062585072865208	23.320292793435947
35	28.006130005615997	24.806152850357424	25.262476560343433	21.92524058368314
36	26.908249807247493	26.108874230179808	21.88012221936663	25.10275374320607
37	29.06022444958451	25.057445006044336	22.10933112500833	23.772999419362822
38	27.430442521678707	25.414774836517317	22.780395404399517	24.374387237404456
39	28.161664620158582	23.987549615922784	22.83198644545342	25.018799318465213
40	26.71825770772057	23.376643155620282	23.403676099641146	26.501423037018
41	25.628563541696415	24.032096861703646	23.68733163901501	26.652007957584928
42	25.136259363964324	21.530026557011908	24.80082241069134	28.53289166833243
43	25.868242953825067	23.604138705655025	25.462177674976445	25.065440665543466
44	26.968788372026616	22.225458560590916	23.6926620786811	27.113090988701373
45	26.755951531073606	23.06481243515425	23.76024443873326	26.418991595038882
46	26.436505896798877	24.27977193333143	24.855078671578287	24.428643498291404
47	25.024510503821734	23.27517442911943	25.671207059025104	26.029108008033734
48	27.399792493598714	22.358719552243066	24.96435268473305	25.277135269425173
49	27.376186260791762	22.137506306100498	25.477217129748613	25.009090303359127
50	25.65026604605119	24.66527694489658	24.75551367352961	24.92894333552262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	1.0
10	2.0
11	119.5
12	237.0
13	674.5
14	1112.0
15	1188.5
16	1265.0
17	1062.0
18	859.0
19	865.5
20	872.0
21	983.5
22	1095.0
23	1180.5
24	1266.0
25	1369.5
26	1473.0
27	1671.5
28	1870.0
29	2348.5
30	2827.0
31	3593.0
32	4359.0
33	5525.0
34	6691.0
35	6895.5
36	7100.0
37	7775.0
38	8450.0
39	10192.5
40	11935.0
41	15205.0
42	18475.0
43	17811.5
44	17148.0
45	19851.0
46	22554.0
47	22675.5
48	22797.0
49	25242.5
50	27688.0
51	29582.5
52	31477.0
53	30909.0
54	30341.0
55	33707.5
56	37074.0
57	39710.0
58	42346.0
59	44834.0
60	47322.0
61	44938.5
62	42555.0
63	43480.5
64	44406.0
65	38056.0
66	31706.0
67	26545.5
68	21385.0
69	16610.0
70	11835.0
71	11022.0
72	10209.0
73	8293.0
74	6377.0
75	5507.0
76	4637.0
77	3450.5
78	2264.0
79	1548.0
80	832.0
81	612.5
82	393.0
83	216.5
84	40.0
85	22.5
86	5.0
87	3.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	525285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.985655406112873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.44328146119987	7.738656158085611
2	9.794475444494505	2.151974642337017
3	3.4519807299067686	1.1376681230189325
4	1.5284372508924549	0.6716353979268397
5	0.7936783003500503	0.4359538155477503
6	0.5285412262156448	0.3483823067477655
7	0.3899074619623609	0.2998372312173392
8	0.3032613593040585	0.2665219833043015
9	0.284199216719232	0.2809903195408207
>10	7.675111773472429	23.97479463529322
>50	3.2024399542508575	24.30699525019751
>100	1.537101861158285	32.06697316694746
>500	0.0537205836481475	3.8858905165767155
>1k	0.013863376425328387	2.433726453258707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2801	0.5332343394538203	No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2408	0.45841781128339854	No Hit
ACCTGAGGGAGTCCTAGCCACATTATTGACAGTCTTGCATTTTGTTTTGT	1632	0.31068848339472854	No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1575	0.2998372312173392	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1185	0.22559182158256946	No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1140	0.21702504354778832	No Hit
ACCTGAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT	1042	0.19836850471648726	No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1001	0.19056321806257553	No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	938	0.17856972881388197	No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	907	0.17266817061214387	No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	859	0.1635302740417107	No Hit
ACCTGAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG	827	0.15743834299475523	No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	820	0.15610573307823372	No Hit
ACCTGAGGGACTGAGATTCTGTTAGGGGGCGTTTACATTGAGAAGAATGA	797	0.15172715763823447	No Hit
ACCTGAGGGGGGGTTTTCTCAGAGTCCATTGCCGGCAATGGGCAAGCCTG	774	0.14734858219823524	No Hit
ACCTGAGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	771	0.14677746366258318	No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	749	0.1425892610678013	No Hit
ACCTGAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	676	0.12869204336693413	No Hit
ACCTGAGGGAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAAC	670	0.12754980629562998	No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	650	0.12374234939128283	No Hit
ACCTGAGGGACCACTGTCAGTTGCAGGTTTCATTTCATTCTCATGGCTAT	641	0.1220289937843266	No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	640	0.12183862093910924	No Hit
ACCTGAGGGGTGGAGTGGATTCTGAAGAGCCCCTTTAGACTAGAGTGTTG	639	0.1216482480938919	No Hit
ACCTGAGGGATGGTGGCATATGCCTTTTTCTCCAGGACTCCGGAGGCAGA	630	0.11993489248693567	No Hit
ACCTGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	618	0.11765041834432736	No Hit
ACCTGAGGCACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCG	613	0.11669855411824057	No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	591	0.11251035152345869	No Hit
ACCTGAGGGACTGTTTCTCCCCAAGGGAAGAGAGGCTAAGCCAGCCACCA	591	0.11251035152345869	No Hit
ACCTGAGGGGGTTAGTTTTAGTGAAGCTTTTGCGTATTTCCTTCTATGGT	589	0.11212960583302399	No Hit
ACCTGAGGGAGGCTGTGGCTTCTTCGTTTGATCCTATAATCATACAGAGT	586	0.11155848729737192	No Hit
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	568	0.10813177608345946	No Hit
ACCTGAGGGAAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG	563	0.10717991185737266	No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	563	0.10717991185737266	No Hit
ACCTGAGGGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGC	553	0.10527618340519908	No Hit
ACCTGAGGGGGTGCCGGGATCTTCGGAGCCGCAACCGACCACACTTCCTT	530	0.10089760796519985	No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	527	0.10032648942954778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.011803116403476208	0.0	0.0	0.0	0.0
7	0.011993489248693567	0.0	0.0	0.0	0.0
8	0.012183862093910924	0.0	0.0	0.0	0.0
9	0.01256460778434564	0.0	0.0	0.0	0.0
10	0.02950779100869052	0.0	0.0	0.0	0.0
11	0.04283389017390559	0.0	0.0	0.0	0.0
12	0.05406588804172973	0.0	0.0	0.0	0.0
13	0.06091931046955462	0.0	0.0	0.0	0.0
14	0.07272242687303083	0.0	0.0	0.0	0.0
15	0.08205069628868138	0.0	0.0	0.0	0.0
16	0.09023672863302779	0.0	0.0	0.0	0.0
17	0.09670940537041796	0.0	0.0	0.0	0.0
18	0.10375320064346022	0.0	0.0	0.0	0.0
19	0.11098736876171983	0.0	0.0	0.0	0.0
20	0.11955414679650095	0.0	0.0	0.0	0.0
21	0.12697868775997792	0.0	0.0	0.0	0.0
22	0.1315476360451945	0.0	0.0	0.0	0.0
23	0.13763956709214997	0.0	0.0	0.0	0.0
24	0.14468336236519222	0.0	0.0	0.0	0.0
25	0.1534405132451907	0.0	0.0	0.0	0.0
26	0.16181691843475446	0.0	0.0	0.0	0.0
27	0.1694318322434488	0.0	0.0	0.0	0.0
28	0.18104457580170766	0.0	0.0	0.0	0.0
29	0.19018247237214084	0.0	0.0	0.0	0.0
30	0.19741664049040045	0.0	0.0	0.0	0.0
31	0.20598341852518157	0.0	0.0	0.0	0.0
32	0.21778653492865777	0.0	0.0	0.0	0.0
33	0.2267340586538736	0.0	0.0	0.0	0.0
34	0.23472971815300264	0.0	0.0	0.0	0.0
35	0.24805581731821774	0.0	0.0	0.0	0.0
36	0.260430052257346	0.0	0.0	0.0	0.0
37	0.2695679488277792	0.0	0.0	0.0	0.0
38	0.2796577096242992	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	7.852572E-4	44.000004	30
TGATACC	20	7.852572E-4	44.000004	20
GTACCGA	20	7.852572E-4	44.000004	23
ACGCCGG	20	7.852572E-4	44.000004	27
TCACCGT	40	8.29823E-9	44.000004	37
TTAATCG	20	7.852572E-4	44.000004	25
CGAGTAT	20	7.852572E-4	44.000004	17
CGAGTAC	20	7.852572E-4	44.000004	34
ACGCATA	20	7.852572E-4	44.000004	12
CTATCGT	20	7.852572E-4	44.000004	33
GCGAAAA	20	7.852572E-4	44.000004	29
ATAATCG	20	7.852572E-4	44.000004	37
CGCATAG	20	7.852572E-4	44.000004	13
CGTCTAT	20	7.852572E-4	44.000004	17
CGATTGC	20	7.852572E-4	44.000004	43
CACGACG	20	7.852572E-4	44.000004	21
TGCGGAA	20	7.852572E-4	44.000004	38
TTATCGC	20	7.852572E-4	44.000004	39
CACCTAT	20	7.852572E-4	44.000004	33
AATGCGA	20	7.852572E-4	44.000004	16
>>END_MODULE