SRR1527437_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415781
Sequences flagged as poor quality	0
Sequence length	52
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2418	0.5815561557646934	No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2013	0.48414910734256733	No Hit
ACCTGAGGGAGTCCTAGCCACATTATTGACAGTCTTGCATTTTGTTTTGTCT	1322	0.3179558469482732	No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1207	0.29029705542100287	No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	914	0.21982726483413145	No Hit
ACCTGAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	870	0.20924477068456712	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	857	0.2061181246858322	No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	822	0.19770023161231515	No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	752	0.18086444546528102	No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	731	0.17581370962117077	No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	721	0.1734085973144516	No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	707	0.1700414400850448	No Hit
ACCTGAGGGACTGAGATTCTGTTAGGGGGCGTTTACATTGAGAAGAATGACA	647	0.1556107662447298	No Hit
ACCTGAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG	623	0.14983849670860383	No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	616	0.1481549180939004	No Hit
ACCTGAGGGGGGGTTTTCTCAGAGTCCATTGCCGGCAATGGGCAAGCCTGGC	613	0.14743338440188467	No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	613	0.14743338440188467	No Hit
ACCTGAGGGAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCA	556	0.13372424425358542	No Hit
ACCTGAGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	532	0.12795197471745942	No Hit
ACCTGAGGGACTGTTTCTCCCCAAGGGAAGAGAGGCTAAGCCAGCCACCATG	525	0.12626839610275603	No Hit
ACCTGAGGGGTGGAGTGGATTCTGAAGAGCCCCTTTAGACTAGAGTGTTGAG	524	0.1260278848720841	No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	512	0.1231417501040211	No Hit
ACCTGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	503	0.12097714902797385	No Hit
ACCTGAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	495	0.11905305918259854	No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	494	0.11881254795192663	No Hit
ACCTGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	489	0.11760999179856704	No Hit
ACCTGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	489	0.11760999179856704	No Hit
ACCTGAGGGGGTTAGTTTTAGTGAAGCTTTTGCGTATTTCCTTCTATGGTTT	488	0.11736948056789512	No Hit
ACCTGAGGGATGGTGGCATATGCCTTTTTCTCCAGGACTCCGGAGGCAGAGT	481	0.1156859019531917	No Hit
ACCTGAGGGACCACTGTCAGTTGCAGGTTTCATTTCATTCTCATGGCTATCT	476	0.11448334579983212	No Hit
ACCTGAGGGAAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAA	471	0.11328078964647254	No Hit
ACCTGAGGCACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGAC	466	0.11207823349311295	No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	465	0.11183772226244104	No Hit
ACCTGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	461	0.11087567733975337	No Hit
ACCTGAGGGATCTGTCTGGGAGTCAGTGCCTATATCCTCACCGCCCCAGCCT	459	0.11039465487840953	No Hit
ACCTGAGGGGCCTCCAGCATTCCCAGGCCTGCGCACTCCTGCTCCTGCCTGT	450	0.10823005380236231	No Hit
ACCTGAGGGGGGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGG	446	0.10726800887967464	No Hit
ACCTGAGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCG	445	0.10702749764900273	No Hit
ACCTGAGGGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG	444	0.1067869864183308	No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	443	0.10654647518765889	No Hit
ACCTGAGGGAGGCTGTGGCTTCTTCGTTTGATCCTATAATCATACAGAGTAT	428	0.10293880672758014	No Hit
ACCTGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	426	0.10245778426623631	No Hit
ACCTGAGGGGGTGCCGGGATCTTCGGAGCCGCAACCGACCACACTTCCTTCT	423	0.10173625057422056	No Hit
ACCTGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	418	0.10053369442086099	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	6.3067826E-4	46.000004	21
AACCGTT	20	6.3067826E-4	46.000004	24
AGCGTAC	20	6.3067826E-4	46.000004	41
AACCGAC	55	1.8189894E-12	46.000004	33
CATATGC	55	1.8189894E-12	46.000004	17
TCACCGT	40	5.5970304E-9	46.000004	37
CTACTAG	20	6.3067826E-4	46.000004	14
CGAAAGT	20	6.3067826E-4	46.000004	27
CGAAAGC	20	6.3067826E-4	46.000004	14
CACGGAT	20	6.3067826E-4	46.000004	32
TAGACTG	20	6.3067826E-4	46.000004	44
CGACGAA	55	1.8189894E-12	46.000004	27
GAATCGC	20	6.3067826E-4	46.000004	17
GTTAGTA	20	6.3067826E-4	46.000004	31
TGTATGG	20	6.3067826E-4	46.000004	29
GTAGGTT	20	6.3067826E-4	46.000004	43
AAGCGTA	20	6.3067826E-4	46.000004	40
CACGAAT	20	6.3067826E-4	46.000004	45
TACGGGA	20	6.3067826E-4	46.000004	13
CGCGACA	20	6.3067826E-4	46.000004	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content