FastQCFastQC Report
Fri 17 Jun 2016
SRR1527436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586263
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC33680.5744861947624189No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG28270.482206791150047No Hit
ACCTGAGGGAGTCCTAGCCACATTATTGACAGTCTTGCATTTTGTTTTGTCT18840.32135747949299204No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT16780.28621966591785597No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT12780.21799090169429078No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC12500.21321488819864123No Hit
ACCTGAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG11820.20161599828063514No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT11140.19001710836262906No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC10540.17978279372909428No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC10260.17500678023344474No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC9960.16988962291667734No Hit
ACCTGAGGGACTGAGATTCTGTTAGGGGGCGTTTACATTGAGAAGAATGACA9660.16477246559990993No Hit
ACCTGAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG9130.15573215434028753No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT8930.15232071612910927No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC8780.1497621374707256No Hit
ACCTGAGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC8750.14925042173904887No Hit
ACCTGAGGGGGGGTTTTCTCAGAGTCCATTGCCGGCAATGGGCAAGCCTGGC8740.14907984982848996No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA7810.13321666214651104No Hit
ACCTGAGGGAGGCTGTGGCTTCTTCGTTTGATCCTATAATCATACAGAGTAT7710.1315109430409219No Hit
ACCTGAGGGAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCA7670.13082865539868627No Hit
ACCTGAGGCACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGAC7580.12929350820365604No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC7390.1260526419030367No Hit
ACCTGAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC7350.12537035426080104No Hit
ACCTGAGGGGTGGAGTGGATTCTGAAGAGCCCCTTTAGACTAGAGTGTTGAG7210.12298234751297626No Hit
ACCTGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT7170.12230005987074061No Hit
ACCTGAGGGATGGTGGCATATGCCTTTTTCTCCAGGACTCCGGAGGCAGAGT7050.12025319694403365No Hit
ACCTGAGGGACCACTGTCAGTTGCAGGTTTCATTTCATTCTCATGGCTATCT7020.1197414812123569No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC6770.11547718344838409No Hit
ACCTGAGGGGGTTAGTTTTAGTGAAGCTTTTGCGTATTTCCTTCTATGGTTT6570.11206574523720583No Hit
ACCTGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG6450.11001888231049886No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG6420.10950716657882215No Hit
ACCTGAGGGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG6400.1091660227577043No Hit
ACCTGAGGGGGTGCCGGGATCTTCGGAGCCGCAACCGACCACACTTCCTTCT6400.1091660227577043No Hit
ACCTGAGGGATCTGTCTGGGAGTCAGTGCCTATATCCTCACCGCCCCAGCCT6270.10694858792043845No Hit
ACCTGAGGGAAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAA6220.10609572836764386No Hit
ACCTGAGGGGCCTCCAGCATTCCCAGGCCTGCGCACTCCTGCTCCTGCCTGT6030.10285486206702453No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.10029628340864083No Hit
ACCTGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC5870.10012571149808192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTT700.046.00000433
CGACGGG351.0184158E-746.00000418
GTTAGTA351.0184158E-746.00000431
GATCATA351.0184158E-746.00000418
CTTATCG351.0184158E-746.00000413
GCGCTAA351.0184158E-746.00000430
TCGACGG351.0184158E-746.00000417
TATCGAC351.0184158E-746.00000415
CGCTTTA351.0184158E-746.00000425
GTTTGCG253.4150922E-546.044
AACCGTT301.8598312E-646.024
AACCGTA551.8189894E-1246.038
CGGCGTA206.308912E-446.040
AACCGGC206.308912E-446.030
TGATACC301.8598312E-646.020
TCGTTAC206.308912E-446.040
ACACGAA206.308912E-446.031
ATTAGTA253.4150922E-546.033
TCACCGT206.308912E-446.037
GTCGATG206.308912E-446.022
Produced by FastQC (version 0.11.5)