##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586263
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.905412758437766	31.0	30.0	31.0	26.0	33.0
2	31.316243733614435	31.0	31.0	33.0	30.0	34.0
3	32.08855411308576	33.0	31.0	34.0	30.0	34.0
4	36.05498214964956	37.0	35.0	37.0	35.0	37.0
5	35.94090877302508	37.0	35.0	37.0	35.0	37.0
6	34.243433407873255	35.0	35.0	36.0	32.0	37.0
7	35.938415011692705	36.0	35.0	37.0	35.0	37.0
8	36.483295039939414	37.0	37.0	37.0	35.0	37.0
9	38.66150174921494	39.0	39.0	39.0	38.0	39.0
10	36.912058240073144	39.0	37.0	39.0	32.0	39.0
11	37.01083813919691	39.0	37.0	39.0	34.0	39.0
12	36.979041829349626	39.0	37.0	39.0	33.0	39.0
13	36.95971944332151	39.0	37.0	39.0	33.0	39.0
14	37.72655787590211	40.0	37.0	40.0	33.0	40.0
15	37.84282310157728	40.0	37.0	40.0	33.0	40.0
16	37.90972311061759	40.0	37.0	40.0	34.0	40.0
17	37.92670354431373	40.0	38.0	40.0	34.0	40.0
18	37.84291521040898	40.0	37.0	40.0	33.0	40.0
19	37.732057114298534	40.0	37.0	40.0	33.0	40.0
20	37.61262436824428	39.0	37.0	40.0	33.0	40.0
21	37.682115364605984	40.0	37.0	40.0	33.0	40.0
22	37.64028089782231	39.0	37.0	40.0	33.0	40.0
23	37.593869986678335	39.0	37.0	40.0	33.0	40.0
24	37.58052614611531	39.0	37.0	40.0	33.0	40.0
25	37.5320308462243	39.0	37.0	40.0	33.0	40.0
26	37.466043396905484	39.0	36.0	40.0	33.0	40.0
27	37.39985808417042	39.0	36.0	40.0	33.0	40.0
28	37.26160784494331	39.0	36.0	40.0	33.0	40.0
29	37.268567178894116	39.0	36.0	40.0	33.0	40.0
30	37.18354902151424	39.0	36.0	40.0	33.0	40.0
31	37.10828246026101	39.0	36.0	40.0	32.0	40.0
32	37.03733477978314	39.0	36.0	40.0	32.0	40.0
33	36.88202905521924	39.0	35.0	40.0	32.0	40.0
34	36.6406749189357	39.0	35.0	40.0	31.0	40.0
35	36.54812260026643	38.0	35.0	40.0	31.0	40.0
36	36.595106633029886	38.0	35.0	40.0	31.0	40.0
37	36.529054366385054	38.0	35.0	40.0	31.0	40.0
38	36.42566561423798	38.0	35.0	40.0	31.0	40.0
39	36.335695754294576	38.0	35.0	40.0	31.0	40.0
40	36.578736846773545	39.0	35.0	40.0	31.0	40.0
41	36.719446732950914	39.0	35.0	40.0	32.0	40.0
42	36.719700885097645	39.0	35.0	40.0	32.0	40.0
43	36.6685071375816	39.0	35.0	40.0	32.0	40.0
44	36.606236450193855	38.0	35.0	40.0	32.0	40.0
45	36.46828300609112	38.0	35.0	40.0	32.0	40.0
46	36.21882499833692	38.0	35.0	40.0	31.0	40.0
47	36.19727494315691	38.0	35.0	40.0	31.0	40.0
48	36.139208853364444	38.0	35.0	40.0	31.0	40.0
49	35.9798264601382	37.0	35.0	40.0	31.0	40.0
50	35.83138966641251	37.0	34.0	40.0	31.0	40.0
51	35.704972000620884	37.0	34.0	40.0	31.0	40.0
52	35.289057982509554	36.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	5.0
16	17.0
17	22.0
18	57.0
19	141.0
20	220.0
21	352.0
22	531.0
23	868.0
24	1248.0
25	1779.0
26	2418.0
27	3384.0
28	4616.0
29	6434.0
30	8682.0
31	12200.0
32	16484.0
33	24831.0
34	43543.0
35	44350.0
36	71298.0
37	116477.0
38	200417.0
39	25885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.49050170316053	0.3757699189612853	0.10626630027820277	0.027462077599984987
8	99.42039664792081	0.3851513740420255	0.16238445885208516	0.03206751918507564
9	98.27705313144442	0.9753301845758644	0.20434514884957777	0.5432715351301378
10	53.7313458294315	36.29940828604227	3.9550508901295154	6.014194994396712
11	35.27819425752606	23.469330317621957	23.136203376300397	18.116272048551586
12	28.254213552620584	18.196952562245954	27.0431529876523	26.505680897481167
13	24.064114569740884	17.112797498733503	30.18781673071642	28.635271200809193
14	23.793587519594446	19.954866672466114	30.690492151133537	25.561053656805903
15	25.113814107320437	20.09337106383995	29.571199274045952	25.22161555479367
16	30.596165884594456	20.79322761286317	24.81275468518395	23.79785181735842
17	30.058352650602206	20.24705635525353	23.250315984464308	26.444275009679956
18	28.214640869370918	21.382382991933653	25.118248976994966	25.284727161700467
19	29.270992711462263	22.569392917513127	22.703291867301875	25.456322503722735
20	27.560327020466925	22.850665998024777	25.133088733213594	24.455918248294708
21	29.499217927790088	21.199188759993383	24.704441521979042	24.597151790237486
22	28.195536815388316	19.755468109022743	25.97451996800071	26.074475107588235
23	27.44740841567692	21.79397301211231	25.637469872736297	25.121148699474467
24	27.46293045953778	22.11447763205251	24.636724473487153	25.78586743492255
25	26.473954522117204	24.60448638239152	23.273513764300322	25.64804533119095
26	27.36263417612914	23.959895132389388	24.455406532563032	24.22206415891844
27	25.718320958341224	21.33598743226163	24.597663505969162	28.348028103427986
28	27.998696830603333	22.571439780439835	23.32707334421582	26.102790044741013
29	28.143853526488964	22.133752257945666	23.381656355594675	26.340737859970698
30	28.177115049047952	20.116227699854843	25.067759691469526	26.63889755962768
31	29.992511893126462	22.501164153289565	23.362552301612073	24.143771651971896
32	31.44441999580393	22.83309709123721	22.091620996037616	23.630861916921244
33	29.815287678055753	21.367202091893912	23.402636700593423	25.414873529456916
34	27.60945173070789	23.87853233105279	24.999189783424843	23.51282615481448
35	28.088929371288994	24.58248260592942	25.314406674137718	22.01418134864387
36	27.03257752919765	25.873370825039277	22.031579683520878	25.0624719622422
37	29.01547598944501	24.962516822654678	22.077975243192903	23.944031944707408
38	27.616786322861923	25.315259533690508	22.676341505433566	24.391612638013996
39	28.031105493609527	23.830601624185732	22.87335206212911	25.264940820075633
40	26.658172185520833	23.218419037189793	23.433169072583464	26.69023970470591
41	25.617171815379784	23.898318672677622	23.615510444970944	26.86899906697165
42	25.246860197556387	21.175138120604576	24.74913136254548	28.82887031929356
43	25.88599314642063	23.467112882784686	25.539902739896597	25.10699123089808
44	26.918294349123173	22.031920827341995	23.75742627455596	27.292358548978875
45	26.775355088074804	22.912071885825984	23.806892128618042	26.505680897481167
46	26.624228375319607	24.27528259501282	24.71433469279146	24.38615433687611
47	25.211210668249574	23.15940115613641	25.523698408393503	26.105689767220515
48	27.472141342707967	22.05136602514571	25.136159027603654	25.34033360454267
49	27.579089930628403	21.82245852117565	25.63849330419965	24.959958243996294
50	25.783820571995847	24.30786182992957	24.76925884799143	25.139058750083155
51	23.31888589250899	22.699880429090697	26.195240020263945	27.785993658136366
52	24.846527923474618	22.85373629241484	25.90714406332994	26.392591720780604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	133.5
12	267.0
13	683.5
14	1154.5
15	1209.0
16	1002.5
17	796.0
18	903.5
19	1011.0
20	985.0
21	959.0
22	1104.5
23	1250.0
24	1341.5
25	1433.0
26	1614.5
27	1796.0
28	2135.5
29	2475.0
30	2877.5
31	3280.0
32	4568.0
33	5856.0
34	6630.5
35	7405.0
36	7136.0
37	6867.0
38	8606.0
39	12962.0
40	15579.0
41	17820.5
42	20062.0
43	19669.5
44	19277.0
45	20564.0
46	21851.0
47	24279.5
48	26708.0
49	27592.5
50	28477.0
51	30734.0
52	32991.0
53	33672.5
54	34354.0
55	36443.0
56	38532.0
57	40563.5
58	42595.0
59	48246.5
60	53898.0
61	50164.0
62	46430.0
63	46983.0
64	42298.0
65	37060.0
66	31626.5
67	26193.0
68	21592.5
69	16992.0
70	14008.5
71	11025.0
72	10463.0
73	9901.0
74	7734.5
75	5568.0
76	4153.0
77	2738.0
78	2077.0
79	1416.0
80	1100.5
81	785.0
82	489.5
83	194.0
84	109.0
85	24.0
86	18.0
87	12.0
88	9.5
89	6.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	586263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.916817196377735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.26031065881092	5.997478947161939
2	10.400566225418931	1.8547989554176199
3	3.906190221133981	1.044923524083901
4	1.757976891881552	0.6270223432145642
5	0.9430713903129544	0.4204597595277205
6	0.6006580457571352	0.32135747949299204
7	0.42275614048511745	0.26387474563463836
8	0.35006503940622846	0.24971727705824862
9	0.27928686203994185	0.22413149047441167
>10	7.8276838319687805	20.20117251131318
>50	4.005662254189303	24.922432423673335
>100	2.130997015839008	35.45968277036074
>500	0.09564618563011708	5.571049170764657
>1k	0.019129237126023414	2.841898601822049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3368	0.5744861947624189	No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2827	0.482206791150047	No Hit
ACCTGAGGGAGTCCTAGCCACATTATTGACAGTCTTGCATTTTGTTTTGTCT	1884	0.32135747949299204	No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1678	0.28621966591785597	No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1278	0.21799090169429078	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1250	0.21321488819864123	No Hit
ACCTGAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	1182	0.20161599828063514	No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1114	0.19001710836262906	No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1054	0.17978279372909428	No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1026	0.17500678023344474	No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	996	0.16988962291667734	No Hit
ACCTGAGGGACTGAGATTCTGTTAGGGGGCGTTTACATTGAGAAGAATGACA	966	0.16477246559990993	No Hit
ACCTGAGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG	913	0.15573215434028753	No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	893	0.15232071612910927	No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	878	0.1497621374707256	No Hit
ACCTGAGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	875	0.14925042173904887	No Hit
ACCTGAGGGGGGGTTTTCTCAGAGTCCATTGCCGGCAATGGGCAAGCCTGGC	874	0.14907984982848996	No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	781	0.13321666214651104	No Hit
ACCTGAGGGAGGCTGTGGCTTCTTCGTTTGATCCTATAATCATACAGAGTAT	771	0.1315109430409219	No Hit
ACCTGAGGGAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCA	767	0.13082865539868627	No Hit
ACCTGAGGCACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGAC	758	0.12929350820365604	No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	739	0.1260526419030367	No Hit
ACCTGAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	735	0.12537035426080104	No Hit
ACCTGAGGGGTGGAGTGGATTCTGAAGAGCCCCTTTAGACTAGAGTGTTGAG	721	0.12298234751297626	No Hit
ACCTGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	717	0.12230005987074061	No Hit
ACCTGAGGGATGGTGGCATATGCCTTTTTCTCCAGGACTCCGGAGGCAGAGT	705	0.12025319694403365	No Hit
ACCTGAGGGACCACTGTCAGTTGCAGGTTTCATTTCATTCTCATGGCTATCT	702	0.1197414812123569	No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	677	0.11547718344838409	No Hit
ACCTGAGGGGGTTAGTTTTAGTGAAGCTTTTGCGTATTTCCTTCTATGGTTT	657	0.11206574523720583	No Hit
ACCTGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	645	0.11001888231049886	No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	642	0.10950716657882215	No Hit
ACCTGAGGGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG	640	0.1091660227577043	No Hit
ACCTGAGGGGGTGCCGGGATCTTCGGAGCCGCAACCGACCACACTTCCTTCT	640	0.1091660227577043	No Hit
ACCTGAGGGATCTGTCTGGGAGTCAGTGCCTATATCCTCACCGCCCCAGCCT	627	0.10694858792043845	No Hit
ACCTGAGGGAAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAA	622	0.10609572836764386	No Hit
ACCTGAGGGGCCTCCAGCATTCCCAGGCCTGCGCACTCCTGCTCCTGCCTGT	603	0.10285486206702453	No Hit
ACCTGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	588	0.10029628340864083	No Hit
ACCTGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	587	0.10012571149808192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.00801687979626891	0.0	0.0	0.0	0.0
7	0.00801687979626891	0.0	0.0	0.0	0.0
8	0.008187451706827824	0.0	0.0	0.0	0.0
9	0.008699167438504561	0.0	0.0	0.0	0.0
10	0.01995691353539282	0.0	0.0	0.0	0.0
11	0.03377323829066477	0.0	0.0	0.0	0.0
12	0.04264297763972825	0.0	0.0	0.0	0.0
13	0.0486129945092902	0.0	0.0	0.0	0.0
14	0.059700168695619546	0.0	0.0	0.0	0.0
15	0.07061677097138998	0.0	0.0	0.0	0.0
16	0.07795136312542324	0.0	0.0	0.0	0.0
17	0.08426252381610301	0.0	0.0	0.0	0.0
18	0.08852682158007584	0.0	0.0	0.0	0.0
19	0.0946674103601967	0.0	0.0	0.0	0.0
20	0.10063742722975866	0.0	0.0	0.0	0.0
21	0.10575458454652605	0.0	0.0	0.0	0.0
22	0.10933659466826322	0.0	0.0	0.0	0.0
23	0.11615947109061973	0.0	0.0	0.0	0.0
24	0.12417635088688865	0.0	0.0	0.0	0.0
25	0.13031693966700952	0.0	0.0	0.0	0.0
26	0.1378221037316017	0.0	0.0	0.0	0.0
27	0.1434509767800458	0.0	0.0	0.0	0.0
28	0.15402643523469842	0.0	0.0	0.0	0.0
29	0.16084931165705493	0.0	0.0	0.0	0.0
30	0.16886619145332385	0.0	0.0	0.0	0.0
31	0.17671249933903385	0.0	0.0	0.0	0.0
32	0.18575281059865623	0.0	0.0	0.0	0.0
33	0.19206397128933603	0.0	0.0	0.0	0.0
34	0.20008085108560492	0.0	0.0	0.0	0.0
35	0.21116802527193426	0.0	0.0	0.0	0.0
36	0.22174348372658687	0.0	0.0	0.0	0.0
37	0.22941921970173795	0.0	0.0	0.0	0.0
38	0.23863010287191927	0.0	0.0	0.0	0.0
39	0.24630583884707033	0.0	0.0	0.0	0.0
40	0.25688129730172293	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTT	70	0.0	46.000004	33
CGACGGG	35	1.0184158E-7	46.000004	18
GTTAGTA	35	1.0184158E-7	46.000004	31
GATCATA	35	1.0184158E-7	46.000004	18
CTTATCG	35	1.0184158E-7	46.000004	13
GCGCTAA	35	1.0184158E-7	46.000004	30
TCGACGG	35	1.0184158E-7	46.000004	17
TATCGAC	35	1.0184158E-7	46.000004	15
CGCTTTA	35	1.0184158E-7	46.000004	25
GTTTGCG	25	3.4150922E-5	46.0	44
AACCGTT	30	1.8598312E-6	46.0	24
AACCGTA	55	1.8189894E-12	46.0	38
CGGCGTA	20	6.308912E-4	46.0	40
AACCGGC	20	6.308912E-4	46.0	30
TGATACC	30	1.8598312E-6	46.0	20
TCGTTAC	20	6.308912E-4	46.0	40
ACACGAA	20	6.308912E-4	46.0	31
ATTAGTA	25	3.4150922E-5	46.0	33
TCACCGT	20	6.308912E-4	46.0	37
GTCGATG	20	6.308912E-4	46.0	22
>>END_MODULE