FastQCFastQC Report
Fri 17 Jun 2016
SRR1527435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3903
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCA2646.764027671022291No Hit
TGCGGAGGGCATGGGTTATATATATTATATATATGATACTGTATATTATT1664.253138611324622No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1112.8439661798616447No Hit
TGCGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAA1032.638995644376121No Hit
TGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTAC892.280297207276454No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA882.2546758903407635No Hit
TGCGGAGGGGGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTT882.2546758903407635No Hit
TGCGGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA862.2034332564693826No Hit
TGCGGAGGGACTGCAAGGGACGTTTAATGTACTCTCATTTATGATGTCAC771.972841404048168No Hit
TGCGGAGGGTGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTT731.870356136305406No Hit
TGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTA621.5885216500128105No Hit
TGCGGAGGAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACG481.2298232129131437No Hit
TGCGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC481.2298232129131437No Hit
TGCGGAGGGGGAAATAATGCTGAAAAGAATTGCAGTCAAAAAAAAAAAAA451.1529592621060722No Hit
TGCGGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC431.1017166282346913No Hit
TGCGGAGGGAGTGCTTGCGGAGGGGGGAATTCGGCCCGGAAACCCTGCGT380.9736100435562387No Hit
TGCGGAGGGGGGCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.8711247758134768No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.7173968741993338No Hit
TGCGGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA240.6149116064565718No Hit
TGCGGAGGGCAAGATAGATTGACCAAAAAAAAAAAAAAAAAAAAAAAAAA240.6149116064565718No Hit
TGCGGAGGGTCAACGCAGAGTGCAGTACTTGCGGAGGGTGCGGAGGGGGC230.5892902895208814No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.5636689725851909No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGT200.5124263387138099No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAAGAAAAAAAAAAAAAAAAA190.48680502177811935No Hit
TGCGGAGGGGGGGTTCTGAGGTGACTGAGAAAAATGTTTCCTTTGTGTGG180.4611837048424289No Hit
TGCGGAGGGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.4611837048424289No Hit
TGCGGAGGGGGCGATGAATGAACACTGCGGGCTTTGATGAAAAAAAAAAA170.4355623879067384No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGCTAAAAAAAAAAA150.3843197540353574No Hit
TGCGGAGGGAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAAA150.3843197540353574No Hit
TGCGGAGGGGCTAGGGATGTAATTTCATGTACTTTAATTCTTGCTAAGGA140.3586984370996669No Hit
TGCGGAGGGATTATAGCACTATAAAAAGCTACTTTACCACCTGGCATACA140.3586984370996669No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGCAAAAAAAAAAAA130.3330771201639764No Hit
TGCGGAGGGGGGTAGACCAAGATCCGAAAAAAAAAAAAAAAAAAAAAAAA130.3330771201639764No Hit
TGCGGAGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.3074558032282859No Hit
TGCGGAGGGCAACATTACTAGACGTACCGAAAAAAAAAAAAAAAAAAAAA120.3074558032282859No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAA120.3074558032282859No Hit
TGCGGAGGGGAGCGGTGGCCGAGTGGTCGAAGGCGCTCGCCTGGAAAAAA110.28183448629259544No Hit
TGCGGAGGGGCATGCGGAGGGGAGTACAGTGCTTGCGGAGGGGGAAAAAA110.28183448629259544No Hit
TGCGGAGGGAGTGGTATCAACTGCGGAGGGGGGGGTAAAAAAAAAAAAAA100.25621316935690497No Hit
TGCGGAGGGTAGCAGCACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.25621316935690497No Hit
TGCGGAGGGCTTGCGGAGGGGTGCAGTGCTTGCGGAGGGATAATACGCAA100.25621316935690497No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGCAAAAAAAAA100.25621316935690497No Hit
TGCGGAGGGAAGCAGTTGGTATCATACTTGCGGAGGGCAAAAAAAAAAAA100.25621316935690497No Hit
TGCGGAGGGCAGAGTGCAGTGCTTGCGGAGGGGGTAAAAAAAAAAAAAAA100.25621316935690497No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTCAAAAAAAAAAAAAA90.23059185242121444No Hit
TGCGGAGGGAAGCAGTGGTATCATTGCGGAGGGGGGCAAAAAAAAAAAAA90.23059185242121444No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAAA90.23059185242121444No Hit
TGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCT90.23059185242121444No Hit
TGCGGAGGGAAGCGTGGTATCCTTGCGGAGGGGCAAAAAAAAAAAAAAAA80.20497053548552396No Hit
TGCGGAGGGGAGTGCAGTGCTTGCGGAGGGAAAAAAAAAAAAAAAAAAAA80.20497053548552396No Hit
TGCGGAGGGCGCAGAGTGCATTGCTTGAGGGAAAAAAAAAAAAAAAAAAA80.20497053548552396No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGATAAAAAAAAAAA70.17934921854983346No Hit
TGCGGAGGGGGGTAGACCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGC70.17934921854983346Illumina Single End Adapter 2 (100% over 31bp)
TGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAAC70.17934921854983346No Hit
TGCGGAGGGATATGCGCGAATTCCTGTAGAACGAACACCAAAAAAAAAAA70.17934921854983346No Hit
TGCGGAGGGGTGGTATCAAGTGCAGTGCTTGCGGAGGGAAAAAAAAAAAA70.17934921854983346No Hit
TGCGGAGGGAGTGCTTACGGAGGGCATTCACGCAGATCGAAAAAAAAAAA70.17934921854983346No Hit
TGCGGAAGGCTGCTTCCCTCAAGAGTAACTATAACCTGCCCATGCACAAG70.17934921854983346No Hit
TGCGGAGGGTGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAA70.17934921854983346No Hit
TGCGGAGGGGTGTTGTTGTTGTGTTGTTGTTGTTGTTAAAAAAAAAAAAA70.17934921854983346No Hit
TGCGGAGGGAGTGCAGTACTTGCGGAGGGAAAAAAAAAAAAAAAAAAAAA70.17934921854983346No Hit
TGCGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA60.15372790161414296Illumina Single End Adapter 2 (100% over 34bp)
TGCGGAGGGGCGTCTTTACGGTGCTTAAAACAAAAAAAAAAAAAAAAAAA60.15372790161414296No Hit
TGCGGAGGGGTGGTATCAATGCTTGCGGAGGGAAAAAAAAAAAAAAAAAA60.15372790161414296No Hit
TGCGGAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.15372790161414296No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGCCAAAAAAAAAAAAAA60.15372790161414296No Hit
TGCGGAGGGGTATCAAGCAGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAA60.15372790161414296No Hit
TGCGGAGAGAAAAAAAAAAAAGAAAAGAAAAAAAAAAAAAAAAAAAAAAA50.12810658467845248No Hit
TGCGGAGGGAGTGGTATCAACTGCGGAGGGGAAAAAAAAAAAAAAAAAAA50.12810658467845248No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTGTTGTTAAAAAAAAAAAA50.12810658467845248No Hit
TGCGGAGGGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAAAAAAAAAAAAA50.12810658467845248No Hit
TGCGGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA50.12810658467845248Illumina Single End Adapter 2 (100% over 34bp)
TGCGGAAGGGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCCC50.12810658467845248Illumina Single End PCR Primer 1 (97% over 46bp)
TGCGGAGGGGACTCGGCGGGGATATGACTGCCTAACCCAAAAAAAAAAAA50.12810658467845248No Hit
TGCGGAGGGCGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAAAAAAAAAAA50.12810658467845248No Hit
TGCGGAGGGGCGCGAATTCCTGTAGAACGAACACTAAAAAAAAAAAAAAA50.12810658467845248No Hit
TGCGGAGGGAAGCGTGGTATCAACGGCGGGGGGAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGAGTGGTATCAACGCTGCGGAGGGGAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGAGACTCGGCGGGGATATGACTGCCTAACGCCAAAAAAAAAA40.10248526774276198No Hit
TGCGGACGGTTTCATAGTCGTACTCGAAGGGATTCTCTGTCCCCTTGGCA40.10248526774276198No Hit
TGCGGAGGGGTAGAATGAACACTAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGAAGCAGTGATATCAACGCAGAAGGGGGGAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGGTTGTTGTTTTGTTGTGTTGTTGTTGTTAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGAGTGGTATCAATGCGGGGGGAAAAAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGGGCCAGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG40.10248526774276198No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGGAGTGCAGTGCTTGCGGAAAAAAAAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGAGAAAAAAAAAAAGAAAAGAAAAAAAAAAAAAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGAGGGGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAAAA40.10248526774276198No Hit
TGCGGATGGGACCAATGACCAGCTATGCACTTGTCCGCTGCTGCAGAGCA40.10248526774276198No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTAAAAAAAAAAAAAAAAAAAAAAA40.10248526774276198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTT206.9297594E-444.011
AGGGGTT253.6779256E-544.06
TGATGCT206.9297594E-444.039
GTTGATG206.9297594E-444.037
AGGGGGA253.6779256E-544.06
GGTTGTT253.6779256E-544.09
TGGTTGA206.9297594E-444.035
AGGGGAC405.2532414E-944.06
AGGGGAA206.9297594E-444.06
ACTGTAT206.9297594E-444.038
TCCTTCG206.9297594E-444.017
GGTTGAT206.9297594E-444.036
GGGTTGT253.6779256E-544.08
GGGCATG206.9297594E-444.07
CAGTCGC351.0134136E-744.040
GGGTTAT206.9297594E-444.013
GGCATGG206.9297594E-444.08
CAGCAGT351.0134136E-744.037
GATACTG206.9297594E-444.035
CCAGCAG351.0134136E-744.036
Produced by FastQC (version 0.11.5)