FastQCFastQC Report
Fri 17 Jun 2016
SRR1527434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACA2358.324477506199079No Hit
TGCGGAGGGCATGGGTTATATATATTATATATATGATACTGTATATTATTAT1495.278072972015586No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA842.975557917109458No Hit
TGCGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAC842.975557917109458No Hit
TGCGGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA672.373361671980163No Hit
TGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACG632.2316684378320937No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA622.196245129295076No Hit
TGCGGAGGGACTGCAAGGGACGTTTAATGTACTCTCATTTATGATGTCACCT622.196245129295076No Hit
TGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACAT441.5586255756287637No Hit
TGCGGAGGGTGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTGC401.4169323414806942No Hit
TGCGGAGGGAGTGCTTGCGGAGGGGGGAATTCGGCCCGGAAACCCTGCGTCA401.4169323414806942No Hit
TGCGGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT321.1335458731845554No Hit
TGCGGAGGAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG301.0626992561105209No Hit
TGCGGAGGGGGAAATAATGCTGAAAAGAATTGCAGTCAAAAAAAAAAAAAAA301.0626992561105209No Hit
TGCGGAGGGAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAAAAA280.991852639036486No Hit
TGCGGAGGGTCAACGCAGAGTGCAGTACTTGCGGAGGGTGCGGAGGGGGCGA270.9564293304994688No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTG270.9564293304994688No Hit
TGCGGAGGGGGGCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.8855827134254338No Hit
TGCGGAGGGGGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTGG220.7793127878143818No Hit
TGCGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG220.7793127878143818No Hit
TGCGGAGGGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.6376195536663124No Hit
TGCGGAGGGGGGGTTCTGAGGTGACTGAGAAAAATGTTTCCTTTGTGTGGAA160.5667729365922777No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.5313496280552604No Hit
TGCGGAGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.4605030109812256No Hit
TGCGGAGGGGGGTAGACCAAGATCCGAAAAAAAAAAAAAAAAAAAAAAAAAA130.4605030109812256No Hit
TGCGGAGGGCAAGATAGATTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.4250797024442083No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAA120.4250797024442083No Hit
TGCGGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT120.4250797024442083No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.4250797024442083No Hit
TGCGGAGGGACCGCTTTTACGATTTCGTCAGTCTGCGCCTCGTTAGCCAGAG110.3896563939071909No Hit
TGCGGAGGGGGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGGGGGGCTTGG110.3896563939071909No Hit
TGCGGAGGGAAGCAGTGGTATCATTGCGGAGGGGGGCAAAAAAAAAAAAAAA110.3896563939071909No Hit
TGCGGAGGGCGACTGGACCACGTGGACACTGAGACTGAAAAAAAAAAAAAAA100.35423308537017356No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGCTAAAAAAAAAAAAA100.35423308537017356No Hit
TGCGGAGGGGTGGTATCAAGTGCAGTGCTTGCGGAGGGAAAAAAAAAAAAAA100.35423308537017356No Hit
TGCGGAGGGGAGTGCAGTGCTTGCGGAGGGAAAAAAAAAAAAAAAAAAAAAA100.35423308537017356No Hit
TGCGGAGGGCTTGCGGAGGGGTGCAGTGCTTGCGGAGGGATAATACGCAAAA100.35423308537017356No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAA100.35423308537017356No Hit
TGCGGAGGGAAGCAGTTGGTATCATACTTGCGGAGGGCAAAAAAAAAAAAAA100.35423308537017356No Hit
TGCGGATGCGCAGTTCCCGCATGGGGTTCTCCTTTTCCCCTTGATCTTGCGC90.3188097768331562No Hit
TGCGGAGGGTGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTTC90.3188097768331562No Hit
TGCGGAGGGGTATCAAGCAGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAAAA90.3188097768331562No Hit
TGCGGAGGGAGTGGTATCAATGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAA90.3188097768331562No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGATAAAAAAAAAAAAA80.28338646829613884No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTGTTGTTAAAAAAAAAAAAAA80.28338646829613884No Hit
TGCGGAGGGCAACATTACTAGACGTACCGAAAAAAAAAAAAAAAAAAAAAAA80.28338646829613884No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGCAAAAAAAAAAAAAA80.28338646829613884No Hit
TGCGGAGGGGCTAGGGATGTAATTTCATGTACTTTAATTCTTGCTAAGGAAG80.28338646829613884No Hit
TGCGGAGGGCAGAGTGCAGTGCTTGCGGAGGGGGTAAAAAAAAAAAAAAAAA70.2479631597591215No Hit
TGCGGAAGGGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCCCAA70.2479631597591215Illumina Single End PCR Primer 1 (97% over 46bp)
TGCGGAGGGAGACTCGGCGGTTATATGACTGCCTAACGCAAAAAAAAAAAAA70.2479631597591215No Hit
TGCGGAGGGATTATAGCACTATAAAAAGCTACTTTACCACCTGGCATACACC70.2479631597591215No Hit
TGCGGAGGGAAGCGTGGTATCCTTGCGGAGGGGCAAAAAAAAAAAAAAAAAA70.2479631597591215No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAA60.21253985122210414No Hit
TGCGGAGGGAGTGCAGTACTTGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAA60.21253985122210414No Hit
TGCGGAGGGCGCAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAA60.21253985122210414No Hit
TGCGGAGGGGTGGTATCAATGCTTGCGGAGGGAAAAAAAAAAAAAAAAAAAA60.21253985122210414No Hit
TGCGGAGGGGAGCGGTGGCCGAGTGGTCGAAGGCGCTCGCCTGGAAAAAAAA50.17711654268508678No Hit
TGCGGAGGGAGTGCTTACGGAGGGCATTCACGCAGATCGAAAAAAAAAAAAA50.17711654268508678No Hit
TGCGGAGGGAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAA50.17711654268508678No Hit
TGCGGAGGGCGAGTGCAGTGCTTGCGGGGGAAAAAAAAAAAAAAAAAAAAAA50.17711654268508678No Hit
TGCGGAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.17711654268508678No Hit
TGCGGAGGGGAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.17711654268508678No Hit
TGCGGAGGGGACTCGGCGGGGATATGACTGCCTAACCCAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTCAAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGATGCGCGAATCCCTGTAGAACGAACACTAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGCAGACTCGGCGGGGATATGACTGCAACGCAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGACATATCCCTCTTCCTGCAGAGTCTCCAGCAGTCGCCGCACA40.14169323414806942No Hit
TGCGGAGGGGCATGCGGAGGGGAGTACAGTGCTTGCGGAGGGGGAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGGCGATGAATGAACACTGCGGGCTTTGATGAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGGCCAGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGAAGCGTGGTATCAACGGCGGGGGGGAAAAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGAGTGGTATCAACGCTGCGGAGGGGAAAAAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGGAAATAATGCCGAAAAGAATTGCAGTCAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGACGCAGACTCGGCGGGGATATGACTGCCTAACGCAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGTGTGCAGTGCTTGCGGAGGGCAAAAAAAAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGAGGGGGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTGC40.14169323414806942No Hit
TGCGGAGGGTAGCAGCACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG40.14169323414806942No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGCCAAAAAAAAAAAAAAAA40.14169323414806942No Hit
TGCGGACGGTTTCATAGTCGTACTCGAAGGGATTCTCTGTCCCCTTGGCACT30.10626992561105207No Hit
TGCGGAGGGTGCGGAGGGCAACGCAGAATGCAGTGCTTGCGGAGGGGCTTGG30.10626992561105207No Hit
TGCGGAGGGGTGCTTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGAGTGGTATCAAGTGCAGTACTTGCGGGGGGAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGATGGGACCAATGACCAGCTATGCACTTGTCCGCTGCTGCAGAGCAGT30.10626992561105207No Hit
TGCGGAGGGCACGGGTTATATATATTATATATATGATACTGTATATTATTAT30.10626992561105207No Hit
TGCGGAGGGAGTGGTATCAACTGCGGAGGGGAAAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGAAGCAGTGATATCAACGCAGAAGGGGGGAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGAGTGCAGTGCTTGCGGGGGGTAGAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGGGTAGACCAAGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGAGCAGTGGTATCATGCGGAGGGGCCAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC30.10626992561105207No Hit
TGCGGAGGGGAGTGCAGTGCTTGCGGAAAAAAAAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGACTCGGCGGGGATATGACTGCCTAACCCCAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGTGTTGTTGTTGTTGTTGTTGTTGTTGTTGAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGGCCAGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGA30.10626992561105207No Hit
TGCGGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGAGACTCGGCGGGTATATGACTGCCTAACGCAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGATATGCGCGAATTCCTGTAGAACGAACACCAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGAGTGGTATCAACTGCGGAGGCGAAAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGATACGCAAAAAAAAAAATCCCGTGTGCTCCTCCCCCAGGCAGC30.10626992561105207No Hit
TGCGGAGGGTAGCAGCACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG30.10626992561105207No Hit
TGCGGAGGGGTGTTGTTGTTGTGTTGTTGTTGTTGTTAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGCAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGCTCAGAGTATCGATGAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGACTCGGCGGGTATATGACTGCCTAACCCAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGAGTGGTATCAACTGCGGAGGGGGGGGGAAAAAAAAAAAAAAAA30.10626992561105207No Hit
TGCGGAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC30.10626992561105207No Hit
TGCGGAGGGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTAAAAAAAAAAAAAA30.10626992561105207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTT205.289084E-446.011
AGGGGAC205.289084E-446.06
AGGGGAA205.289084E-446.06
ACTGTAT205.289084E-446.038
CAGTCGC205.289084E-446.040
GGGTTAT205.289084E-446.013
GGCATGG205.289084E-446.08
CAGCAGT205.289084E-446.037
TATATGA205.289084E-446.030
CCAGCAG205.289084E-446.036
GTCGCCG205.289084E-446.042
CCTGCAG205.289084E-446.024
TTATTAT205.289084E-446.046
CTCTTCC205.289084E-446.019
CTCCAGC205.289084E-446.034
GGACATA205.289084E-446.09
CCGCACA205.289084E-446.046
TCCAGCA205.289084E-446.035
GTATATT205.289084E-446.041
ATTATTA205.289084E-446.045